Labshake search
Citations for Beckman :
651 - 700 of 1121 citations for CTLA 4 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... brought up to 3 ml using sterile 20 mM Tris-HCl pH 7.5 and pelleted at 40,000g and 4°C for 60 minutes (TLA100.3 rotor, Optima Max-XP ultracentrifuge, Beckman Coulter, Inc.). Finally ...
-
bioRxiv - Cell Biology 2022Quote: ... 1 M sucrose) and centrifuged at 100,000 RPM for 60 minutes at 4°C in a TLA100.3 rotor (Beckman Coulter, 349490) with 3.5 mL polycarbonate tubes (Beckman Coulter ...
-
bioRxiv - Molecular Biology 2022Quote: ... The sucrose gradient was centrifuged at 24,400 rcf for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter), and nuclei at the bottom of the gradient were resuspended in PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... and concentrated by 20% sucrose gradient ultracentrifugation in an Optima XPN-100 ultracentrifuge at 26,000 rpm at 4°C for 2 hours in a SW32 Ti swinging bucket rotor (Beckman-Coulter). Viral particles were resuspended in PBS and stored at −80°C.
-
bioRxiv - Neuroscience 2020Quote: ... The V and VI fractions were diluted to a total volume of 12 mL with dfPBS and centrifuged at 140,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter), and each pellet were resuspend with 30 μl of dfPBS ...
-
bioRxiv - Plant Biology 2021Quote: ... The thylakoid membranes with different densities were analytically separated at 125,000 × g and 4°C for 1 h (SW 41 Ti, Beckman Coulter).
-
ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... UV-inactive SARS-CoV-2 was then partially-purified using a 20% sucrose cushion centrifuged at 175,000 x g for 3-4 hr using SW32Ti rotor (Beckman Coulter). The sample that has passed through the sucrose was collected and concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore) ...
-
bioRxiv - Molecular Biology 2020Quote: ... The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K, Beckman Coulter). The pellet was then resuspended in the required volume of PBS before being aliquoted and stored at −70°C.
-
bioRxiv - Microbiology 2020Quote: ... OBs were loaded on to a discontinuous 35–60% (w/w) sucrose gradient and centrifuged at 20000 rpm and 4°C for 1 hour in a SW41 rotor (Beckman). The band containing the OBs was removed ...
-
bioRxiv - Microbiology 2021Quote: ... The membrane fraction was prepared using ultracentrifugation (45,000 rpm, 4 °C, 1h; Optima-90 ultracentrifuge, Beckman-Coulter, Brea, CA, USA) and washed once in 200 mM ABC buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... The sucrose loaded heavy MLVs were centrifuged at 180,000 x g for 30 min at 4°C using Optima TL benchtop Ultracentrifuge (Beckman Coulter). The resulting pellets were buffer exchanged by resuspension into the assay buffer comprising 50 mM HEPES pH 7.5 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Insoluble material was removed by centrifugation (100,000 x g; 30 min; 4°C; 45 Ti rotor; Optima XPN-80; Beckman Coulter). The cell lysate supernatant was passed through a Ni+2-NTA resin column (Qiagen) ...
-
bioRxiv - Molecular Biology 2021Quote: ... The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g; 4°C; 30 min; 45Ti rotor; Optima XPN-80, Beckman Coulter). The membrane pellet was resuspended in solubilization buffer ...
-
bioRxiv - Molecular Biology 2021Quote: ... Ultra-centrifugation was performed (160,000 x g; 16 hours; 4°C; SW 32 Ti rotor; Optima XPN-80, Beckman Coulter) and 1mL fractions were collected and analyzed by BN-PAGE ...
-
bioRxiv - Microbiology 2021Quote: ... Concentrated supernatants were then subjected to ultracentrifugation at 160,000 x g for 2 hours at 4°C using a Beckman Ti 45 rotor (Beckman, Germany). Supernatants were completely removed and the pellet containing the crude MV fraction was resuspended in 400 μL of chilled PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... The gradient was subjected to high-speed centrifugation at 100,000 x g for 2 h at 4° C using a SW41Ti rotor (Beckman Coulter). 12 fractions of 1ml each were collected from the top of the gradient ...
-
bioRxiv - Neuroscience 2022Quote: ... Lentiviral supernatant was collected 48 hours after transfection and centrifuged for 2 hours by ultracentrifugation at 25,000 RPM at 4°C (Beckman Coulter). Pellets were resuspended in DPBS and frozen in -80°C until used ...
-
bioRxiv - Neuroscience 2022Quote: ... 10 mM Tris-HCl) and centrifugated at 24,000 rpm for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS ...
-
bioRxiv - Molecular Biology 2019Quote: ... followed by ultracentrifugation at 100,000 g for 2h in polyallomer tubes at 4 °C in a SW40 rotor (Beckman Coulter). Pelleted material was washed two times in filtered PBS at 100,000 g for 2h and re-suspended in PBS ...
-
bioRxiv - Biochemistry 2019Quote: ... 10,000 events were then immediately analyzed from 1 of the 4 150 µL aliquots using a CytoFLEX benchtop cytometer (Beckman). All subsequent chase time points were collected using this initial analysis as a reference ...
-
bioRxiv - Cell Biology 2019Quote: ... and spun down at 4° C for 10 minutes at 12096×g using an Avanti J-25 centrifuge (Beckman Coulter). The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads ...
-
bioRxiv - Biochemistry 2019Quote: ... The supernatant was then slowly mixed with an equal volume of 4 M ammonium sulphate (2 M final concentration) at 4°C for 1 hour and spun at 10 K rpm using a JA-17 rotor (Beckman). Pellets containing RAD51 were suspended in 25 ml of resuspension buffer (0.5 M KCl ...
-
bioRxiv - Molecular Biology 2019Quote: ... and then centrifuged for 150 min at 39,000 rpm and 4°C in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41Ti rotor without brake ...
-
bioRxiv - Biochemistry 2019Quote: ... The supernatant from 2,000 × g was transferred to 30mL conical tube and then centrifuged at 10,000 × g for 10 min at 4°C (# Avanti J-E JA25-50 Beckman Coulter). The supernatant from this spin was filtered through a 0.22-μm mesh filter (# SLGP033RS EMD Millipore ...
-
bioRxiv - Biochemistry 2019Quote: ... The V and VI fractions were then diluted separately to a total volume of 12mL with dfPBS and centrifuged at 100,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter). The final pellet from each fraction was resuspended in 30 μL of dfPBS ...
-
bioRxiv - Cell Biology 2020Quote: ... The supernatant of MNase treated chromatin were overlaid onto the sucrose gradient and spun at 32000 rpm (124,436 rcf) for 13 h at 4 °C using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80.
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM EGTA) and spun at 32000 rpm (124,436 rcf) and 4 °C for 13 h using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80 ...
-
bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 4°C for 3 h at 100,000 g by using Avanti J-30XP (Beckman, USA), and the fractions were collected using a Brandel Fractionation System (USA ...
-
bioRxiv - Microbiology 2021Quote: ... The treated cells were pelleted by centrifugation at 12,000×g at 4 °C for 20 min (Beckman Allegra X-15R) and the supernatant was discarded ...
-
bioRxiv - Molecular Biology 2021Quote: ... cell lysates were cleared by centrifugation at 4 °C for 5 minutes at 16000 x g and loaded onto ice-cold 15-50 % sucrose-CHX gradients and ultracentrifuged at 4 °C for 2 hours at 274 000 x g in a SW-41Ti rotor (Beckman). Gradients were fractionated and monitored at an absorbance of 254 nm with a fraction collector (BioComp Instruments ...
-
bioRxiv - Plant Biology 2020Quote: ... The insoluble cell debris was removed via centrifugation for 30 min at 4°C and 20,000 rpm (JA-25.50, Beckman Coulter®). The supernatant was incubated for 1 h with low agitation and with 1.2 ml Glutathione-sepharose (GE-Healthcare ...
-
bioRxiv - Plant Biology 2020Quote: ... the cells were harvested by centrifuging for 30 min at 4°C and 6,000 rpm (JA-10, Beckman Coulter®). The cells were resuspended in 1x PBS (pH 7.3 ...
-
bioRxiv - Immunology 2020Quote: ... Then the concentrated supernatant was ultracentrifuged at 110,000 g for 1 hour at 4°C (Beckman Coulter L100k, company name) to sediment the vesicular fraction into a pellet ...
-
bioRxiv - Neuroscience 2019Quote: ... The supernatant was layered onto a 20% sucrose cushion prepared in the same lysis buffer and then centrifuged at 186,000 x g for 2 h at 4 °C in a TLA-55 rotor ultracentrifuge (Beckman Coulter). RNA was isolated from the ribosome-enriched pellet using TRIzol LS reagent according to manufacturer’s instructions (Invitrogen).
-
bioRxiv - Molecular Biology 2020Quote: ... Centrifugation was conducted at 40,000 rpm for 2 hours at 4°C in a SW-41 Ti rotor (Beckman Coulter), and fractions were collected from the top of the gradient (#1 to #20) ...
-
bioRxiv - Genomics 2021Quote: ... The tubes were then balanced and centrifuged in a Beckman Coulter L7-65 Ultracentrifuge at 25,000rpm at 4°C for 2hr using a Beckman Coulter SW28 swing bucket rotor (Beckman Coulter). The supernatant was carefully removed via aspiration ...
-
bioRxiv - Genomics 2019Quote: ... 37°C for 30 min then cooled to 4°C before proceeding to a 2.2x Agencourt RNAClean XP cleanup (Beckman Coulter). The RNA was eluted in 11.5 μL nuclease-free water ...
-
bioRxiv - Biochemistry 2019Quote: ... Ribosomes were pelleted via centrifugation at 63,000 x g for 16-18 hours at 4°C using a Type 80 Ti rotor (Beckman Coulter). Ribosome pellets were dissolved in resuspension buffer (20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Biochemistry 2019Quote: ... Fractions containing 40S and 60S ribosomal subunits as identified via absorption at 254 nM were pelleted via centrifugation at 63,000 x g for 20 hours at 4°C using a Type 80 Ti rotor (Beckman Coulter). Ribosomal subunits were resuspended in storage buffer (30 mM HEPES-KOH pH 7.4 ...
-
bioRxiv - Biochemistry 2019Quote: ... The homogenate was centrifuged for 10 min at 10,000×g at 4°C in a Ti-70 rotor and Optima XPN-90 ultracentrifuge (Beckman Coulter) and the supernatant was removed ...
-
bioRxiv - Molecular Biology 2020Quote: ... by centrifuging at 63,000 x g for 18 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). Pellets were resuspended and these samples were analysed by mass spectrometry.
-
bioRxiv - Immunology 2021Quote: ... Ultracentrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for two hours at 4°C using a Beckman SW28 rotor (Beckman Coulter) was performed to pellet the viruses through the sucrose cushion while soluble egg proteins were removed ...
-
bioRxiv - Cell Biology 2020Quote: ... The ultracentrifugation (UC) steps were performed in refrigerated conditions (4°C) with rotors 50.4Ti or 70Ti (Beckman-Coulter, California, US).
-
bioRxiv - Biochemistry 2020Quote: ... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
-
bioRxiv - Physiology 2021Quote: ... the supernatant was ultracentrifuged at 100,000 g for 70 min at 4°C using an MLA-55 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples were ultracentrifuged at 120,000 × g for 90 minutes at 4 °C in an Optima XPN-80 ultracentrifuge (Beckman Coulter) using a SW55 Ti swinging-bucket rotor (Beckman Coulter) ...
-
bioRxiv - Biochemistry 2021Quote: ... and cellular debris was removed by centrifugation at 90 000 x g for 20 min at 4°C (45 Ti rotor, Beckman). Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor ...
-
bioRxiv - Cell Biology 2020Quote: ... This crude mitochondrial pellet was further purified on a discontinuous percoll gradient (buffer composition 15/23/40% sucrose) by centrifugation (40,000 x g, 1hr, 4°C) in an SW41 rotor (Beckman Coulter) and enriched mitochondria were collected from the 23-40% interface (adapted from [76]) ...
-
bioRxiv - Cell Biology 2020Quote: ... Tubes were centrifuged twice at 100,000 x g for 70 min at 4°C with a Beckman ultracentrifuge (32Ti rotor from Beckman Coulter). Supernatants were removed and the pellets resuspended in phosphate-buffered saline (PBS ...
-
bioRxiv - Microbiology 2020Quote: ... Microbial cells were collected from to 2-3 L of water samples by centrifugation at 12,000 × g and 4°C for 20 min in a high-speed centrifuge (Beckman, USA). Total genomic DNA was extracted using AxyPrep™ Genomic DNA Miniprep Kit (Axygen ...