Labshake search
Citations for Beckman :
101 - 150 of 901 citations for Breast Cancer Type 1 Susceptibility Protein BRCA1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 56,000 rpm in a Type 70 Ti rotor (Beckman Coulter) at 4°C for 1 hour ...
-
bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with the following p24 antibodies: HIV-1 p24 clone 28B7-APC (Medimabs) and HIV-1 p24 clone KC57-PE (Beckman Coulter). Acquisition was performed on the Fortessa LSR flow cytometer (BD Bioscience ...
-
bioRxiv - Immunology 2020Quote: ... HIV-1 p24-PE antibody (KC57-RD1, #6604667) for intracellular staining was purchased from Beckman Coulter ...
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation (60 minutes, 40,000 RPM) in a Type 45 Ti rotor (Beckman Coulter), filtered and loaded onto a HisTrap HP column (GE Healthcare) ...
-
bioRxiv - Cell Biology 2021Quote: ... supernatants were spun for 70 minutes at 200,000 × g and 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
-
bioRxiv - Plant Biology 2022Quote: ... 330,000 x g / 60,000 RPM for 4.5 hours using a TY 70.1Ti rotor (Type 70.1 Ti Rotor, Beckman Coulter, USA) loaded into an Optima XE-100 Ultracentrifuge (Beckman Coulter ...
-
bioRxiv - Neuroscience 2023Quote: ... Nuclei for the cell type of origin were sorted either on a MoFlo Astrio EQ sorter (Beckman Coulter), BD FACSAria II (Becton-Dickinson) ...
-
bioRxiv - Biochemistry 2023Quote: ... Membrane fractions were isolated by ultracentrifugation at 40,000 x g in a type 45 Ti rotor (Beckman Coulter). Membrane fractions were further homogenised and washed twice with high-salt buffer (10 mM HEPES pH 7.5 ...
-
bioRxiv - Neuroscience 2023Quote: RNA was extracted from wild-type mouse livers using the Agencourt RNAdvance Tissue kit (Beckman Coulter, Brea, CA), and cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
-
bioRxiv - Microbiology 2023Quote: ... Not solubilised protein was removed by a second pelleting at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The solubilized and denatured protein was loaded on a 5 ml HisTrap HP column (Cytiva) ...
-
bioRxiv - Immunology 2021Quote: ... following by staining for 15 minutes at room temperature with a 1:100 dilution of a fluorescent anti-HIV-1 GAG antibody (KC57-FITC, Beckman Coulter reference 6604665) in permeabilization buffer ...
-
bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation (12,000x□g for 15□min at 4°C) and membranes were then pelleted at 45,000 rpm in a Type 45 Ti rotor (Beckman) for 3□h at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by ultracentrifugation at 200000 g for 120 minutes (Type 70 Ti rotor, Optima L-80 XP, Beckman Coulter). The pellet was washed in PBS and collected by ultracentrifugation at 200000 g for 120 minutes [17] ...
-
bioRxiv - Cell Biology 2022Quote: ... Sonicate was supplied with 0.5 mM PMSF and clarified by centrifugation at 40,000 rcf for 60 min in a Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 15 ml Ni-NTA agarose (Qiagen ...
-
bioRxiv - Microbiology 2019Quote: ... Conditioned media was then centrifuged at 36,000 × g for 3 hours at 4ºC (Type 45 Ti rotor, Beckman Coulter). OMV-containing pellets were resuspended in 2 mL ASW.
-
bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 1.5 mL of anti-Flag M2 affinity gel (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2020Quote: ... supernatants were centrifuged at 100,000 × g for 190 min in ultracentrifugation using type 70 Ti rotor (Oprima XL-80K, Beckman) to pellet the EVs (Fig ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were lysed via sonication (Branson Digital Sonifier) and clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman) at 4°C ...
-
bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
-
bioRxiv - Cell Biology 2021Quote: ... by three passes through an EmulsiFlex-C5 (Avestin, Inc.) and then clarified by centrifugation at 40,000 rpm in a Type 45ti rotor (Beckman) for 1.5 hours at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 1.5 mL of anti-Flag M2 affinity gel (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 ml of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by 12,000 rpm for 30 min using a fixed angle type 70 Ti rotor (Beckman Coulter, Brea, CA) and polycarbonate aluminum bottles with cap assembly (Beckman Coulter) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Whole-cell lysates were spun at 35k rpm for 45 min in a Type 45 Ti rotor (Beckman Coulter), and the supernatant was decanted and filtered through a 0.45 μm cellulose acetate membrane ...
-
CM1-driven assembly and activation of Yeast γ-Tubulin Small Complex underlies microtubule nucleationbioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by ultracentrifugation at 40,000 x g for 30 min in a Type 45Ti rotor (Beckman-Coulter). Xrcc4-Spc110164-207 was then purified by NiNTA affinity chromatography followed by addition of 3C protease overnight at 4° C to cleave the 6His tag ...
-
bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 0.9% NaCl and spun at 110,000 × g for 2 hours at 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL glutathione-sepharose (Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
-
bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells were lysed using a microfluidizer and cell lysate was clarified by centrifugation at 45,000 rpm (235,000 rcf) for 60 minutes using a Type 45 Ti rotor (Beckman-Coulter). The fusion protein was captured using GST affinity chromatography ...
-
bioRxiv - Neuroscience 2023Quote: ... Fractions were washed by diluting with 7 mL of PBS and ultracentrifuged at 100,000 x g (Type 70.1 Ti rotor, Beckman Coulter) for 2 h ...
-
bioRxiv - Neuroscience 2023Quote: ... Then AAVs were purified by ultracentrifugation (OptimaXE-90, Type 70 Ti rotor with 32.4mL OptiSealTM tubes, Beckman Coulter, USA). with 58400 rpm for 145 minutes in 18°C with Iodixanol density gradient solutions (15% ...
-
bioRxiv - Neuroscience 2024Quote: ... Ultracentrifugation was performed for 2.5 h at 18 °C at 350,000 × g in a type 70 Ti rotor (Beckman Coulter). The interface between the 60 and 40% gradient was extracted along with the 40% layer ...
-
bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 minutes in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 ml of IgG Sepharose 6 Fast Flow beads (Cytiva ...
-
bioRxiv - Molecular Biology 2023Quote: Proteins were collected by centrifugation (Beckman-Coulter JA-14 rotor ...
-
bioRxiv - Microbiology 2021Quote: ... 50 μL of each cell sample was mixed with 1 μL of KC57-FITC antibody that identifies the HIV-1 Gag and p24 (Beckman Coulter Life Sciences, USA) and incubated at 4°C for 20 min ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 s off at 30 % amplitude for 10 min and cell debris was removed by centrifugation at 130,000 ×g for 45 min with a Type 70 Ti Rotor (Beckman Coulter). The supernatant was filtered through a 0.22 μm filter (Sigma ...
-
bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by centrifugation at 37,000 rpm for 40 min in a Type 45 Ti fixed-angle rotor (Beckman Coulter). Pre-equilibrated Ni2+-nitrilotriacetic acid (NTA ...
-
bioRxiv - Cell Biology 2021Quote: ... The cell lysate was clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman Coulter) at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... the sample was centrifuged for 16 h at 4°C and 100,000 rcf using a type 70 Ti rotor (Beckman Coulter). The pellet was gently washed with 500 μl of storage buffer (lysis buffer C + 10% (v/v ...
-
bioRxiv - Molecular Biology 2022Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
-
bioRxiv - Microbiology 2022Quote: Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 minutes at 4°C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 x 175 cm² flasks ...