Labshake search
Citations for Beckman :
51 - 100 of 155 citations for Argininosuccinic Acid Barium Salt 2H2O Arginine 13C6 99%; 15N4 99% 90%+ Cp since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... The resulting supernatant was then centrifuged at 100,000 x g for 90 minutes using the 70Ti rotor (Beckman). The pellet was washed with PBS followed by another centrifugation at 100,000 × g for 90 minutes ...
-
bioRxiv - Immunology 2022Quote: ... filtered through a 0.22-μm filter and concentrated by ultracentrifugation at 20,000 rpm (Beckman Coulter Optima XE-90) for 2 hours at 4°C ...
-
bioRxiv - Genetics 2022Quote: ... were then spun at 19,000rpm/90 minutes/4°C using an ultracentrifuge and a SW28 hanging rota (Beckman). All supernatant was removed and each viral pellet was resuspended with 300 µl of 1% Bovine Serum Albumin (Tocris Bioscience ...
-
bioRxiv - Microbiology 2020Quote: ... The DNA from these PCR reactions was purified using 90 uL Agencourt AMPure XP beads (Beckman Coulter A63880) before being pooled in equimolar ratios and sequenced using an Illumina MiSeq Paired-end 250-cycle kit.
-
bioRxiv - Microbiology 2019Quote: ... EVs were precipitated at 100,000 × g for 90 min at 4 °C (50.2Ti rotor, Beckman Coulter, Brea, CA) and suspended in PBS ...
-
bioRxiv - Biochemistry 2021Quote: ... The virus-containing filtrate was then further purified using an ultracentrifuge (Optima XPN-90 Ultracentrifuge, Beckman Coulter, USA) with a cycle of 1,000 rpm (116 g ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor ...
-
bioRxiv - Neuroscience 2022Quote: ... were then spun at 19,000rpm/90 minutes/4°C using an ultracentrifuge and a SW28 hanging rota (Beckman). All supernatant was removed and each viral pellet was resuspended with 300 µl of 1% bovine serum albumin (Tocris Bioscience ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatants were filtered through 450nm pore size filters and concentrated by ultracentrifugation (Beckman SW32Ti, 25,000 rpm, 90 min), resuspend in OptiMEM on a platform shaker for at least 60 min at 4°C and followed by pipetting up and down 20 times ...
-
bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
-
bioRxiv - Genomics 2020Quote: ... Salt or Proteinase K treated samples were purified using 1.8X volumes of AMPure XP (Beckman Coulter) beads prior to PCR amplification ...
-
bioRxiv - Genomics 2020Quote: ... Salt or Proteinase K treated samples were purified using 1.8X volumes of AMPure XP (Beckman Coulter) beads prior to PCR amplification ...
-
bioRxiv - Immunology 2021Quote: ... Lysates were centrifuged at 200,000 xg for 90 min in an Optima XPN-80 ultracentrifuge (Beckman Coulter, IN, USA) and filtered supernatants were loaded on immunoaffinity columns ...
-
bioRxiv - Microbiology 2020Quote: ... supernatants were harvested and filtered through a 0.45μm filter and centrifuged at 27,000 rpm for 3h at 4°C with a SW41 rotor and Optima L-90 centrifuge (Beckman). Pellets were resuspended in PBS prior to use for western blot analysis.
-
bioRxiv - Microbiology 2022Quote: ... sonicated using a sonicator (Smurt, Microtec) and ultracentrifuged at 100,000 x g for 60 minutes at 4°C (Optima XE-90, Beckman). Protein concentration of the bacterial membrane suspension was determined with Bio-Rad protein assay (BioRad).
-
bioRxiv - Microbiology 2019Quote: ... Virions present in the supernatant were pelleted by ultracentrifugation (22 krm, 90 min, 4°C, Beckman-Coulter rotor SW28) on a 5-mL cushion of 30% sucrose ...
-
bioRxiv - Biochemistry 2021Quote: ... Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor, Beckman).
-
bioRxiv - Microbiology 2020Quote: ... Particles from media were concentrated by ultracentrifugation through a 20% (wt/wt) sucrose cushion (90 min at 27,000 rpm in a Beckman SW32 rotor ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor, Beckman Avanati J-25, Beckman Coulter). The resulting pellets were washed again and resuspended in 500 μl potassium phosphate buffer ...
-
bioRxiv - Microbiology 2022Quote: ... Glycoproteins in the supernatants were pelleted by centrifugation at 100,000×g at 4°C for 90 min using a TLA-55 rotor (Beckman). The resultant pellets ...
-
bioRxiv - Molecular Biology 2022Quote: ... The post-nuclear supernatant was then centrifuged at 32,500 RPM (∼128,000xg) for 30 min in an Optima XE-90 ultracentrifuge (Beckman Coulter). The supernatant (cytosol fraction ...
-
bioRxiv - Microbiology 2023Quote: ... Then 90 ul of the mix was transferred to a fresh tube and cleaned with SPRIselect beads (Beckman Coulter) using a two-sided (1.2x-0.5X ...
-
bioRxiv - Neuroscience 2023Quote: ... Then AAVs were purified by ultracentrifugation (OptimaXE-90, Type 70 Ti rotor with 32.4mL OptiSealTM tubes, Beckman Coulter, USA). with 58400 rpm for 145 minutes in 18°C with Iodixanol density gradient solutions (15% ...
-
bioRxiv - Cell Biology 2024Quote: ... EVs were collected in a refrigerated ultracentrifuge at 100,000-110,000 xg for 90 min (Optima XPN-100 ultracentrifuge using a SW28 or SW32Ti rotor, Beckman Coulter). EVs were subject to 2 washes in 50 mM HEPES pH 7.4 containing 150 mM NaCl with recovery at 100,000 xg for 90 min ...
-
bioRxiv - Biochemistry 2024Quote: ... Cells debris was removed by low-speed centrifugation for 30 minutes and the supernatant were further subjected to high-speed centrifugation at 150,000 g for 90 minutes using a Ti-45 rotor (Beckman), and the membrane component was collected ...
-
bioRxiv - Immunology 2021Quote: ... the culture medium containing the lentiviral particles was collected and ultracentrifuged at 25,000 rpm for 90 min at 4 °C using a Beckman SW28 ultracentrifuge rotor (Beckman Coulter) for the obtention of concentrated virus ...
-
bioRxiv - Molecular Biology 2020Quote: ... This supernatant was ultracentrifuged at 100,000×g for 90 min in a SW-28Ti Rotor Swinging Bucket rotor (Beckman Coulter) and resuspended in PBS ...
-
bioRxiv - Microbiology 2021Quote: ... Archaella were pelleted from the supernatant by ultracentrifugation (60,000 × g, 90 min, 4 °C; Beckman Optima LE-80K, rotor 70Ti), resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g ...
-
bioRxiv - Microbiology 2022Quote: Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 minutes at 4°C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 x 175 cm² flasks ...
-
bioRxiv - Molecular Biology 2020Quote: ... The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K, Beckman Coulter). The pellet was then resuspended in the required volume of PBS before being aliquoted and stored at −70°C.
-
bioRxiv - Microbiology 2021Quote: ... The membrane fraction was prepared using ultracentrifugation (45,000 rpm, 4 °C, 1h; Optima-90 ultracentrifuge, Beckman-Coulter, Brea, CA, USA) and washed once in 200 mM ABC buffer ...
-
bioRxiv - Cell Biology 2022Quote: ... EVs were collected by ultracentrifugation at 20,000g for 60 min using Optima XL-90 Ultracentrifuge and 90Ti rotor (Beckman Coulter).
-
bioRxiv - Microbiology 2020Quote: ... The cell lysate was collected and centrifuged at 100,000 x g for 1 h in an ultracentrifuge (Model XL-90, Beckman Coulter). The supernatant was collected ...
-
bioRxiv - Biochemistry 2019Quote: ... The homogenate was centrifuged for 10 min at 10,000×g at 4°C in a Ti-70 rotor and Optima XPN-90 ultracentrifuge (Beckman Coulter) and the supernatant was removed ...
-
bioRxiv - Biochemistry 2021Quote: ... Samples were ultracentrifuged at 120,000 × g for 90 minutes at 4 °C in an Optima XPN-80 ultracentrifuge (Beckman Coulter) using a SW55 Ti swinging-bucket rotor (Beckman Coulter) ...
-
bioRxiv - Biochemistry 2021Quote: ... and cellular debris was removed by centrifugation at 90 000 x g for 20 min at 4°C (45 Ti rotor, Beckman). Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor ...
-
bioRxiv - Microbiology 2023Quote: ... The filtrate was ultracentrifuged at 100,000 ×g at 4 °C for 2 h using an Optima XE-90 (Beckman Coulter) to collect EMVs ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was then transferred to polycarbonate tubes and centrifuged at 100,000 × g and 4°C for 1 h in a XL-90 ultracentrifuge (Beckman Coulter) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Virus particles were collected by centrifugation at 32,000rpm for 2 hours at 4°C using the Optima XE-90 ultracentrifuge (Beckman Coulter). Virus was aliquoted and stored at -80°C until further use ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The gradients were centrifuged at 154,300× g for 18 hours at 18°C (SW41Ti rotor, Optima XE-90 Ultracentrifuge [Beckman Coulter]). After centrifugation ...
-
bioRxiv - Cell Biology 2023Quote: ... Small EVs were isolated by ultracentrifugation at 120,000 x g and 4°C for 120 min using Optima XE-90 equipment with a SW32Ti rotor (Beckman Coulter). Supernatants were discarded using a pipette ...
-
bioRxiv - Cell Biology 2023Quote: ... the fifth virus was freeze-thawed three times and ultracentrifuged at 23 k rpm at 4 °C for 1.5 hours in the Super-centrifuge Optima XE-90 Ultracentrifuge (Beckman Coulter) using Ultra-Clear Centrifuge Tubes (Beckman Coulter) ...
-
bioRxiv - Biochemistry 2021Quote: ... The UV-Visible spectrum of each sample was recorded for both tannic acid and gallic acid for both pH’s using a DU730 UV-Visible Spectrometer (Beckman Coulter, Pasadena, CA). The spectra were recorded from 250-400 nm with 0.2 nm data intervals ...
-
bioRxiv - Neuroscience 2021Quote: ... This was incubated for 1 hour at room temperature while rotating before centrifugation at 100,000 x g for 90 minutes at 4°C using the Optima™ MAX-XP Ultracentrifuge (Beckman Coulter). The supernatant was then collected (sarkosyl-soluble fraction) ...
-
bioRxiv - Neuroscience 2021Quote: ... The reaction volume was increased to 50 μl and the tagmentated DNA was purified using 90 μl of Ampure XP beads (Beckman Coulter) and an elution volume of 40 μl of H2O ...
-
bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
-
bioRxiv - Biochemistry 2022Quote: ... sucrose gradient and the RCII complexes were separated by ultracentrifugation (40,000×g,15 h) using an Optima XPN-90 Ultracentrifuge (Beckman Coulter, USA). The separated RCII/PSI fraction was collected and then washed repeatedly using the glycerol- and DM-free elution buffer and concentrated to 0.2 mg Chl/ml which corresponded to approximately 0.6 mg protein/ml.
-
bioRxiv - Physiology 2019Quote: ... MBS was added to the homogenate until a volume of 2.5 mL was reached then mixed with 2.5 mL of 90% sucrose in MBS and 4mL of this mixture was added to an ultracentrifuge tube (Beckman Coulter 344061). A sucrose gradient was generated by adding 4 mL 35% sucrose followed by 4mL of 5% sucrose ...
-
bioRxiv - Microbiology 2019Quote: ... saliva EVs were pelleted by ultracentrifugation at 100,000 × g for 90 min at 4 °C (Optima™ Max-XP, Beckman Coulter). The EVs were washed in 2 ml PBS and pelleted again at 100,000 × g for 90 min and suspended in PBS ...
-
bioRxiv - Immunology 2020Quote: ... and the boundary layers between the two sucrose layers were harvested after ultracentrifugation (90 min, 51,000 rpm, 18 °C, Optima™ L90K centrifuge, rotor type: 70.1 Ti, tubes: 16 * 76 mm, Beckman Coulter, USA). These fractions were subsequently mixed with 6 M CsCl (AppliChem ...