Labshake search
Citations for Beckman :
401 - 450 of 641 citations for Anti Cathepsin S Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... resuspended with DPBS containing 2 % FBS, and median fluorescent intensities of TMRM (Ex 561 nm, Em 584) were measured with CytoFlex S flow cytometer (Beckman Coulter). For each studied cell line four independent experiments with three technical repeats were performed as described above ...
-
bioRxiv - Synthetic Biology 2023Quote: EGFP transfected cells and HEK-293-loxP-GFP-RFP cells before and after PLP coincubation were measured with a CytoFLEX S cytometer (Beckman Coulter) using 488 nm laser with a 525/50 filter for measuring EGFP expression and a 561 nm laser with a 610/20 filter for measuring RFP ...
-
bioRxiv - Microbiology 2024Quote: ... Stained cells were then washed once with PBS and ROS levels were analyzed using a CytoFLEX S flow cytometer (Beckman Coulter) with lasers at wavelengths of 405 nm (violet) ...
-
bioRxiv - Cancer Biology 2024Quote: ... CRC cells were then cocultured at a 1:1 ratio with BMDCs for 4 hours and analyzed for uptake of CRC DNA by flow cytometry on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
-
bioRxiv - Genetics 2024Quote: ... and EGFP fluorescence was measured by FACS on either a SA3800 Spectral Cell Analyzer (Sony Biotechnology) or a CytoFLEX S Flow Cytometer (Beckman Coulter). Typically ...
-
bioRxiv - Microbiology 2024Quote: ... cultures were diluted 1:20 in filtered saline solution (NaCl 0.9%) and fluorescent intensity was measured by flow cytometry using a CytoFLEX-S cytometer (Beckman Coulter, USA). The measurement of each biological replicate is the result of the mean of the fluorescence intensity of 30 000 events per sample ...
-
bioRxiv - Developmental Biology 2022Quote: ... The samples were resuspended in 200 μl and stained on ice 30 minutes with APC conjugated anti-CD56 antibody (Beckman Coulter) and subsequently washed ...
-
bioRxiv - Cancer Biology 2019Quote: Cells were stained for 30 min (minutes) with the anti-human monoclonal antibody CD11b-PE (IM2581U, Beckman Coulter, Brea, CA, USA), CD14-FITC (555397 ...
-
bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
-
bioRxiv - Microbiology 2020Quote: ... Duplicates of samples without EdU were analysed by FACS and stained in permeabilized samples with 0.05% Saponin using anti-Gag antibody conjugated to Rhodamine (Beckman Coulter, #6604667) diluted 1:500 in PBS-BSA 0.5% ...
-
bioRxiv - Immunology 2022Quote: ... and the expression of the Gag- p24 viral protein was detected with a PE-anti-p24 antibody (clone KC57 RD1; Beckman Coulter). Cell viability was determined using an aqua viability dye for flow cytometry (LIVE/DEAD® Fixable Aqua Dead Cell Stain kit ...
-
bioRxiv - Immunology 2022Quote: ... cells were seeded into pre-coated wells with anti-TCR pan-γδ clone IMMU510 antibody (5ug/ml) (Beckman Coulter, Krefeld, Germany) and IL-2 (100IU/ml) ...
-
bioRxiv - Pathology 2023Quote: ... the proportion of HIV-1-infected cells was determined by intracellular staining of the viral protein Gag-p24 with a Mouse anti-p24-FITC antibody (clone KC57, Beckman Coulter).
-
bioRxiv - Microbiology 2021Quote: ... 1.3 µM final concentration of SYTOX™ Green was added to each sample prior to loading into a CytoFLEX S Flow Cytometer (Beckman Coulter). 100,000 gated events were recorded for each condition ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were washed twice with PBS/1% BSA and resuspended in 500 µl PBS/1% BSA for analysis on a CytoFLEX S flow cytometer (Beckman Coulter, Australia). FITC was excited using a 488 nm laser and emission read at 525 nm using a 525/40 band-pass filter with a gain setting of 300 ...
-
bioRxiv - Microbiology 2021Quote: ... cells were diluted 100-fold in PBS and green fluorescence (488 nm) and were measured using a cytoFLEX S (Beckman Coulter, USA). Data were analyzed using CytExpert (Beckman Coulter ...
-
bioRxiv - Cell Biology 2022Quote: ... pelleted and then re-suspended in ice-cold 0.2% FCS in DPBS for flow cytometry analysis using CytoFLEX S (Beckman Coulter, Bucks, UK). Cells were excited at 488 nm and the emission fluorescence was collected at 535 nm ...
-
bioRxiv - Molecular Biology 2019Quote: ... Stained cells were washed twice in 500ul cold differentiation media and assayed on a 4 laser CytoFLEX S Flow Cytometer (Beckman Coulter, B75408). Data was processed in FlowJo V10 ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Green versus white cells were distinguished using single cell fluorescence estimated using a CytoFLEX S flow cytometer (Beckman Coulter Inc., CA, USA) by measuring the intensity of chlorophyll Fluorescence (excitation 488nm ...
-
bioRxiv - Cancer Biology 2022Quote: ... The rate of cell apoptosis was performed by Cyto-FLEX S Flow Cytometer and was analyzed using FlowJo 7.6 software (Beckman Coulter, CA, USA).
-
bioRxiv - Cancer Biology 2024Quote: ... Apoptotic cells were analyzed with the Ebioscience™ Annexin V Apoptosis Detection Kit APC according to the kit instructions (Cat. 88-8007-74, Thermo Fisher Scientific. Samples were read through the CytoFLEX S (Beckman Coulter) instrument using the APC laser to detect the Annexin-V+ cells ...
-
Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... anti-CD105 and anti-HLA-ABC antibodies (suppl. Table 1) and analysed by flow cytometry (Cytoflex; Software Cytexpert 2.3, Beckman-Coulter, Krefeld, Germany). Upon passage 3 ...
-
bioRxiv - Bioengineering 2021Quote: The following antibodies were used for flow cytometry staining in a 96-well format: PE-labelled anti-human CD4 (Beckman Coulter, #A07752), APC-labelled anti-human CD8 (Beckman Coulter ...
-
bioRxiv - Microbiology 2023Quote: ... Cell pellets were permeabilized for intracellular staining via resuspension in 0.5% Triton X-100 in PBS for 10 min and were subsequently resuspended in a 1:500 dilution of anti-p24-FITC KC57 antibody (Beckman Coulter #6604665) in 1% BSA in PBS for 1 h at room temperature in the dark ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Frequencies of competitors were determined by passing mixed population samples from the start (F0) and end (F1) of the experiment through a Cytoflex S flow cytometer (Beckman Coulter, Indianapolis, Indiana). Approximately 50,000 cells per sample were gated by forward and side scatter and competitors were differentiated through comparison of fluorescence by excitation at 561 nm and emission through a 610/20 BP nm filter ...
-
bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
-
bioRxiv - Cell Biology 2022Quote: ... re-dissolved in the different media and run on a CytoFLEX S flow cytometer with the CytExpert 2.4 software (Beckman coulter, Indianapolis, IN, USA). The results were analyzed in FlowJo v ...
-
bioRxiv - Cell Biology 2023Quote: ... The stained cells were resuspended in PBS and analyzed using CytoFLEX S or Galios® flow cytometers (Beckman-Coulter Corp., Hialeah, FL, USA). Data analyses were performed using FlowJo version 10.
-
bioRxiv - Immunology 2021Quote: ... following by staining for 15 minutes at room temperature with a 1:100 dilution of a fluorescent anti-HIV-1 GAG antibody (KC57-FITC, Beckman Coulter reference 6604665) in permeabilization buffer ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
-
bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
-
bioRxiv - Molecular Biology 2020Quote: Binding to CD16 was studied by a competitive assay with an anti-CD16-PE/3G8 antibody conjugated to phycoerythrin (Beckman Coulter, Brea, CA, USA) and unlabelled test and control antibodies using Jurkat-CD16 cells.
-
bioRxiv - Cancer Biology 2020Quote: ... BSA-coated ultracentrifugation tubes prefilled a with 10 ml sucrose buffer cushion were carefully overlaid with the dissolved nuclei pellet and centrifuged for 60 minutes at 13,000 g (Beckman, Brea, CA, Avanti S-25, JA25.50 rotor). The supernatant was discarded and the isolated nuclei were dissolved in 1 ml nuclei buffer (0.44 M sucrose ...
-
bioRxiv - Immunology 2019Quote: Purified antibodies (BioLegend, BD, Beckman Coulter) were conjugated to stable metal isotopes using commercially available polymeric chelators (“X8,” Fluidigim ...
-
bioRxiv - Cell Biology 2021Quote: ... and PAR1 antibodies (Beckman Coulter, #IM2584) and developed by chemiluminescence.
-
bioRxiv - Immunology 2020Quote: ... recombinant protein and CD14 antibody (Beckman) were used at 20 μg/ml ...
-
bioRxiv - Biophysics 2023Quote: ... 4b4 antibody was purchased from Beckman Coulter.
-
bioRxiv - Immunology 2023Quote: ... VersaComp Antibody Capture Kit (Beckman Coulter) was used to set compensation to correct for spectral overlap ...
-
bioRxiv - Immunology 2020Quote: ... and with Cyto-stat tetrachrome (FITC anti-CD45, PECy5 anti-CD3, PE anti-CD4, and ECD anti-CD8, Beckman Coulter, catalog number 660713). Unstimulated cells and pokeweed mitogen-stimulated cells were additionally stained with AF750 anti-CD19 (Beckman Coulter ...
-
bioRxiv - Cell Biology 2023Quote: ... 4B4 integrin inhibiting antibody (Beckman Coulter, 41116015), vinculin (Sigma ...
-
bioRxiv - Immunology 2023Quote: ... The following antibodies were purchased from Beckman Coulter ...
-
bioRxiv - Cancer Biology 2023Quote: ... anti-CD45RA (Beckman), anti-CD3 (BD Bioscience) ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in p24 primary antibody solution (1:100 dilution of p24-FITC antibody clone KC57 (Beckman Coulter) diluted in perm/wash buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibodies were as follows: PECAM1 (IM2409, Beckman Coulter), MCAM (A07483 ...
-
bioRxiv - Immunology 2020Quote: ... PE anti-CD300a (E59.126) and PE anti-2B4 (clone C1.7) from Beckman Coulter ...
-
bioRxiv - Immunology 2023Quote: ... cells were washed with 1% FBS in PBS antibody staining buffer and stained with the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
-
bioRxiv - Biophysics 2020Quote: ... with a rotor Anti-60 and anti-50 (Beckman Coulter, Palo Alto, USA) and double-sector cells of optical path length 12 and 3 mm equipped of Sapphire windows (Nanolytics ...
-
bioRxiv - Bioengineering 2021Quote: ... CD45-APC-A750 antibodies (Beckman Coulter, Brea, California, USA), or corresponding isotype controls in matched concentration ...
-
bioRxiv - Microbiology 2022Quote: ... and stained with α-p24 KC57 antibody (Beckman Coulter) for an additional 45 min at room temperature ...