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Citations for Beckman :
151 - 200 of 396 citations for Adenovirus Type 5 Hexon Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Microbiology 2023Quote: ... Archaella were precipitated from the supernatant at 142400 g using Beckman Coulter Opima XE-90 ultacentrifuge and the fixed-angle titanium rotor Type 45 Ti (Beckman Coulter). The tafi remained in the supernatant and could be precipitated either during many hours of high-speed centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... The UC step was an EV depletion step where the FBS was ultracentrifuged at 118,000 × g (Type 45 Ti rotor, 38,800 rpm, Beckman Coulter, Brea, CA) for 18 h at 4°C followed by a filtration step (0.22 μm) ...
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bioRxiv - Immunology 2024Quote: ... The gradient section containing assembled encapsulins was removed via aspiration and nanocontainers were isolated in a final ultracentrifugation step in a Type 70Ti rotor (Beckman Coulter) spinning at 68,000 rpm for 2 hours at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter, USA). The supernatant was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter, USA). The supernatant was discarded ...
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bioRxiv - Molecular Biology 2024Quote: Density gradient ultracentrifugation using a SW Type 55 Ti swinging bucket rotor in LM-70 ultracentrifuge (Beckman Coulter, Brea, CA, USA) was achieved using OptiPrepTM iodixanol solution (60% iodixanol ...
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bioRxiv - Microbiology 2021Quote: ... and 1.3 mL of 5% (v/v) glycerol in TNE buffer) in 5 mL thin-walled Ultraclear centrifuge tubes (Beckman Coulter). Viral nucleocapsids were pelleted by centrifugation at 23,000 rpm in an SW 55 Ti rotor for 1 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
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bioRxiv - Immunology 2021Quote: ... The collected media were filtered with a 0.45-μm filter and ultracentrifuged at 21,000 rpm using a Type 45 Ti rotor (Beckman Coulter, Brea, CA, USA), and the pellets were resuspended in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Neuroscience 2023Quote: ... Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter, 337922). Titer was calculated using digital droplet PCR (ddPCR ...
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bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
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bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...
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bioRxiv - Synthetic Biology 2019Quote: ... prior to CsCl density gradient ultracentrifugation (1.5 M CsCl) in Optima™ L90K centrifuge (rotor type: 70.1 Ti, tubes: 16 * 76 mm, Beckman Coulter, Brea, California, USA) for 65 h at 48,400 rpm and 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Nuclei used to isolate each cell type were analyzed and sorted using a MoFlo Astrios EQ cell sorter (Beckman Coulter, Brea, CA, USA). Nuclei pellets were centrifuged for 10 min at 1500g and processed immediately for gDNA extraction using a QIAamp DNA micro kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was centrifuged at 4°C for 1 hour and 40 minutes at 182,753 x g (38,000 rpm in a Beckman Coulter Type 42.2 Ti rotor).
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bioRxiv - Microbiology 2020Quote: EV-depleted medium was obtained by overnight ultracentrifugation of DMEM supplemented with 20% FBS at 100,000xg in a Type 45 Ti rotor (Beckman Coulter, K-factor 1042.2). After ultracentrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... supernatants were centrifuged at 100 000 x g for 70 min in ultracentrifugation using type 70 Ti rotor (Oprima XL-80K, Beckman, Brea, CA, USA) to pellet the EVs ...
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bioRxiv - Bioengineering 2023Quote: ... The diluted sample was then centrifuged using a Beckman ultracentrifuge (Optima L90K) with two cycles of 1h10 at 110,000 x g at 4°C (rotor type 70Ti, Beckman Floor Ultracentrifuge Optima L90K). Between them ...
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bioRxiv - Cell Biology 2024Quote: ... and centrifuged at 16,500 × g (14,500 rpm) at 4°C for 20 min using a Type Ti 45 fixed-angle rotor (Beckman Coulter, k-factor 1.275) to isolate large EVs ...
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bioRxiv - Physiology 2019Quote: ... Agencourt AMPure XP PCR purification kit (5 ml Beckman Coulter Part No ...
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bioRxiv - Cell Biology 2022Quote: ... we used a CytoFLEX 5 flow cytometer (Beckman Coulter) and the CytExpert version 2.3 software ...
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bioRxiv - Neuroscience 2022Quote: ... anti-CD90 (IM1839U / Beckman Coulter / F15-42-1-5), anti-CD146 (A07483 / Beckman Coulter / TEA1-34) ...
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bioRxiv - Immunology 2023Quote: ... KIR2DL1/S1 (Beckman Coulter, clone EB6B: PE-Cy5-5) was performed at room temperature for 30 minutes in PBS (w/o Ca or Mg ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of complete Neurobasal medium were added and centrifuged for 5 min at 335 x g (Beckman Coulter Allegra X-12R centrifuge). The complete Neurobasal medium consisted of ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 minutes at 4°C with TLA100 rotor (Beckman). 100 µg cell lysate was brought to a final volume of 250 µl BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2) and spun in an SW41Ti rotor (Beckman) for 20 hours at 30,900 rpm ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were sedimented in the SW40Ti rotor (Beckman Coulter) at 100,000 × g for 17 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: Supernatant containing proteins and membrane were ultracentrifuged at 200,000xg (Beckman Coulter Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... proteins and liposomes were mixed in polycarbonate tubes (Beckman Coulter) and incubated for 30 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... briefly mixed and equilibrated for ∼30 min (Albumax equilibration was conducted in a 5% CO2 incubator to maintain pH 7.4) after which they were subjected to ultracentrifugation at 37°C using a sealed rotor (Beckman Rotor type 42.2 Ti; 223,000 x g) for 4.2 h to separate proteins ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes (TEMP) and labeled with Viakrome 808 (Beckman Coulter) and fluorochrome-labeled antibodies against CD4 ...
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bioRxiv - Immunology 2022Quote: ... anti-NKG2A-APC (clone Z199; 5 μL; cat. # A60797) from Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant was transferred into a 5 mL polypropylene tube (Beckman Coulter), and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Molecular Biology 2019Quote: ... 500 mM sucrose) in a 5 × 44 mm micro centrifuge tube (Beckman Coulter) and sedimented by centrifugation (12000 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... Crosslinking reactions were loaded on 600µl 5∼35% glycerol gradients (Beckman 344090 tubes) in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors) ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were centrifugated at 1000 g for 5 min at 4°C (Beckman Coulter Microfuge 22R ...
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted at 200 x g 5 min in Allegra centrifuge (Beckman) and then resuspended in complete RPMI ...
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bioRxiv - Bioengineering 2019Quote: ... 5′ cDNAs were purified using the Agencourt AMPure XP Kit (Beckman Coulter Genomics) and analysed by capillary electrophoresis before sequencing the single-end libraries using the Illumina NextSeq 500 system and a MID 150 Kit with 75 bp read length.
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bioRxiv - Cancer Biology 2020Quote: ... 3 and 5 and counted using a particle counter (Z1; Beckman Coulter, Inc.). Experiments were carried out in triplicate ...
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bioRxiv - Cell Biology 2023Quote: ... and then spun for 5 minutes in a chilled airfuge (Beckman Coulter, 340401) at around 80,000 rpm ...
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bioRxiv - Microbiology 2024Quote: ... gradient (25 – 39 % OptiPrep, 309600 x g, 5 h, SW60Ti rotor (Beckman Coulter). Purification of VLPs was realized accordingly ...
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bioRxiv - Molecular Biology 2024Quote: ... Discontinuous sucrose gradient was prepared in 5 ml ultracentrifuge tubes (Beckman Coulter, C14279) with 2 ml of 1.2 M sucrose solution (1.2 M sucrose ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein complexes were then fractionated by centrifugation in an SW 41Ti rotor (Beckman) at 38,000 rpm ...