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Citations for Beckman :
101 - 150 of 280 citations for Adenovirus Type 40 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 53,000 RPM for 40 min at 4°C using a Ti70 rotor (Beckman). The supernatant was discarded and the pellet was transferred to a 2 mL tube in 50 μL PBS per g initial grey matter and resuspended by sonication using a Hielscher S26D11X10 Vial-Tweeter-Sonotrode in 0.5 s pulses at 100% amplitude for a total accumulated power of 200 W ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation (60 minutes, 40,000 RPM) in a Type 45 Ti rotor (Beckman Coulter), filtered and loaded onto a HisTrap HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... The solution was centrifuged for 1 h at 4°C and 45,000 rpm (Type 45 Ti rotor, Beckman) and the supernatant was bound to 2 mL (CV ...
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bioRxiv - Biophysics 2019Quote: ... and the lysate was clarified by centrifugation for 1 h at 192,838 g in a Type 50.2 Ti rotor (Beckman) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... supernatants were spun for 70 minutes at 200,000 × g and 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Plant Biology 2022Quote: ... 330,000 x g / 60,000 RPM for 4.5 hours using a TY 70.1Ti rotor (Type 70.1 Ti Rotor, Beckman Coulter, USA) loaded into an Optima XE-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei for the cell type of origin were sorted either on a MoFlo Astrio EQ sorter (Beckman Coulter), BD FACSAria II (Becton-Dickinson) ...
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bioRxiv - Biochemistry 2023Quote: ... Membrane fractions were isolated by ultracentrifugation at 40,000 x g in a type 45 Ti rotor (Beckman Coulter). Membrane fractions were further homogenised and washed twice with high-salt buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from wild-type mouse livers using the Agencourt RNAdvance Tissue kit (Beckman Coulter, Brea, CA), and cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Biophysics 2020Quote: ... The cell lysate was centrifuged at 18000 rpm for 40 minutes using a floor centrifuge (Beckman, USA). The supernatant ...
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bioRxiv - Cancer Biology 2021Quote: ... The gradients were centrifuged for 2.5 hours at 37,000 rpm (223 000g) (SW 40 TI Beckman rotor) and then placed on an Automated Density Fractionation System to collect fractions ...
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bioRxiv - Biophysics 2021Quote: ... 1,5mL of each dilution (60%, 40% and 20% iodixanol) were successively layered in a SW55Ti tube (Beckman) and the pellet of VLPs obtained after ultracentrifugation on sucrose cushion was loaded on the top ...
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bioRxiv - Biochemistry 2019Quote: ... 40 µL of functional complexes was spun in tabletop ultracentrifuge (Beckman TLX, TLA 120.2 rotor, 60,000 rpm) at 25°C for 30 min ...
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bioRxiv - Neuroscience 2023Quote: ... containing 0.25 M sucrose and 40% Optiprep (OP) and placed in ultra-clear centrifuge tubes (Beckman, 344059). Discontinuous OP gradients were prepared by layering 2 mL of 60 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... but 2.5 mL for 5%) and ultracentrifuged at 100,000 x g (SW 40 Ti rotor, Beckman Coulter) for 16 h using ultra-clear tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 3 h at 40 000 rpm in a SW41swing out rotor (Beckman Coulter). The separated gradient was then fractionated by upward displacement with 55% sucrose solution using a gradient fractionator (Brandel Inc. ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation (12,000x□g for 15□min at 4°C) and membranes were then pelleted at 45,000 rpm in a Type 45 Ti rotor (Beckman) for 3□h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by ultracentrifugation at 200000 g for 120 minutes (Type 70 Ti rotor, Optima L-80 XP, Beckman Coulter). The pellet was washed in PBS and collected by ultracentrifugation at 200000 g for 120 minutes [17] ...
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bioRxiv - Cell Biology 2022Quote: ... Sonicate was supplied with 0.5 mM PMSF and clarified by centrifugation at 40,000 rcf for 60 min in a Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 15 ml Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Conditioned media was then centrifuged at 36,000 × g for 3 hours at 4ºC (Type 45 Ti rotor, Beckman Coulter). OMV-containing pellets were resuspended in 2 mL ASW.
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bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 1.5 mL of anti-Flag M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... supernatants were centrifuged at 100,000 × g for 190 min in ultracentrifugation using type 70 Ti rotor (Oprima XL-80K, Beckman) to pellet the EVs (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed via sonication (Branson Digital Sonifier) and clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman) at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Cell Biology 2021Quote: ... by three passes through an EmulsiFlex-C5 (Avestin, Inc.) and then clarified by centrifugation at 40,000 rpm in a Type 45ti rotor (Beckman) for 1.5 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 1.5 mL of anti-Flag M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 ml of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 ml of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 12,000 rpm for 30 min using a fixed angle type 70 Ti rotor (Beckman Coulter, Brea, CA) and polycarbonate aluminum bottles with cap assembly (Beckman Coulter) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Whole-cell lysates were spun at 35k rpm for 45 min in a Type 45 Ti rotor (Beckman Coulter), and the supernatant was decanted and filtered through a 0.45 μm cellulose acetate membrane ...
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CM1-driven assembly and activation of Yeast γ-Tubulin Small Complex underlies microtubule nucleationbioRxiv - Biochemistry 2021Quote: ... Lysate was cleared by ultracentrifugation at 40,000 x g for 30 min in a Type 45Ti rotor (Beckman-Coulter). Xrcc4-Spc110164-207 was then purified by NiNTA affinity chromatography followed by addition of 3C protease overnight at 4° C to cleave the 6His tag ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 0.9% NaCl and spun at 110,000 × g for 2 hours at 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL glutathione-sepharose (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was subjected to a second round of ultracentrifugation at 100,000 x g for 1 hour at 4°C in a Type 45Ti rotor (Beckman) to pellet cell membranes ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were lysed using a microfluidizer and cell lysate was clarified by centrifugation at 45,000 rpm (235,000 rcf) for 60 minutes using a Type 45 Ti rotor (Beckman-Coulter). The fusion protein was captured using GST affinity chromatography ...
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bioRxiv - Neuroscience 2023Quote: ... Fractions were washed by diluting with 7 mL of PBS and ultracentrifuged at 100,000 x g (Type 70.1 Ti rotor, Beckman Coulter) for 2 h ...
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bioRxiv - Neuroscience 2023Quote: ... Then AAVs were purified by ultracentrifugation (OptimaXE-90, Type 70 Ti rotor with 32.4mL OptiSealTM tubes, Beckman Coulter, USA). with 58400 rpm for 145 minutes in 18°C with Iodixanol density gradient solutions (15% ...
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bioRxiv - Neuroscience 2024Quote: ... Ultracentrifugation was performed for 2.5 h at 18 °C at 350,000 × g in a type 70 Ti rotor (Beckman Coulter). The interface between the 60 and 40% gradient was extracted along with the 40% layer ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 minutes in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 ml of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2022Quote: ... cleared supernatants were loaded on a linear 10%–40% sucrose gradient and ultracentrifuged in a SW41Ti rotor (Beckman) for 1 hour and 30 min at 270,000 g at 4°C in a Beckman Optima LE-80K Ultracentrifuge ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were ultracentrifuged at 95,000 rpm at 4°C for 1 h and 40 min using a TLA100.2 rotor (Beckman). Supernatants were saved if needed for further analysis ...
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bioRxiv - Cell Biology 2022Quote: ... passed through a 40 μm strainer and subjected to analysis on a CytoFlex S or Gallios (Beckman Coulter). Analysis was performed using Kaluza software (Beckman Coulter) ...