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Citations for Beckman :
151 - 200 of 905 citations for ADAM Metallopeptidase With Thrombospondin Type 1 Motif 6 ADAMTS6 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... by centrifuging at 63,000 x g for 18 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). Pellets were resuspended and these samples were analysed by mass spectrometry.
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bioRxiv - Neuroscience 2021Quote: ... 40% and 60% iodixanol gradient and was spun at 184,000 x g (50,000 rpm in a Beckman Type 70Ti rotor) for 3 hours 20 minutes at 10°C ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant from this step was then subjected to ultracentrifugation at 200,000g for 35 min (Beckman Type 45 Ti rotor) to collect microsomes ...
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bioRxiv - Microbiology 2020Quote: ... Whole-cell lysates were then spun at 35,000 rpm for 45 min in a Type 45 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were spun for 24 h at 4°C at 50000 rpm in a Type 70 Ti rotor (Beckman Coulter) through a 40 % (w/v ...
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bioRxiv - Immunology 2020Quote: ... all CCMV CP preparations were purified two times using an ultracentrifuge and 30% sucrose cushion – first with additional 0.5% Triton X-100 and the second time without Triton X-100 (4 h, 50 000 rmp, 4°C; Type 70Ti rotor, Beckman) and the pellet was then dissolved in buffer A ...
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bioRxiv - Bioengineering 2022Quote: ... the conditioned media was subjected to an additional centrifugation step of 118,000 × g for 2 h in a Type 70 Ti ultracentrifuge rotor (Beckman Coulter). The resulting EV pellet was resuspended in 1X PBS and placed into a Nanosep 300 kDa MWCO spin column (Pall ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were cleared by centrifugation at 50,000 × g for 40 min in a Type 45 Ti fixed-angle rotor (Beckman Coulter). Pre-equilibrated Pierce High Capacity Streptavidin agarose resin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Lysates were cleared by centrifugation at 50,000 × g for 40 min in a Type 45 Ti fixed-angle rotor (Beckman Coulter). Preequilibrated Ni2+-nitrilotriacetic acid (NTA ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Ribosomes were pelleted by centrifugation at 63,000g for 16–19 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). For all ultracentrifugation steps indicated here ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... were pelleted by centrifugation at 63,000g for 20 hrs at 4°C in a Type 80 Ti rotor (Beckman Coulter) as described above ...
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bioRxiv - Microbiology 2022Quote: ... The sample-containing tube was spun at 347,000 × g and 20°C for 3 hours using Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 45 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: Sedimentation of high molecular weight species before aggregation was carried out using a Type 50.2 Ti fixed-angle rotor in an Optima Ultracentrifuge (Beckman Coulter). Samples were centrifuged at 40,000 rpm (average 146,000 g) ...
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bioRxiv - Neuroscience 2023Quote: ... the crude lysate was subjected to iodixanol gradient (15%, 25%, 40% and 60%) ultracentrifugation using a Type 70Ti rotor (Beckman) at 490000 × g ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 40,000 rpm for 35 min at 4°C (Type 45 Ti Rotor, Beckman Coulter). The resulting supernatant was mixed with 1ml pre-washed IgG Sepharose 6 FastFlow beads (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted extract was then centrifuged at 31,200 rpm for 30 minutes at 4°C in a Type 70 Ti rotor (Beckman Coulter). The supernatant was filtered through a 0.45 µm syringe filter (Millex-GP PES membrane (Millipore SLHP033RS ...
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bioRxiv - Biochemistry 2023Quote: ... by dounce homogenization (20 strokes).The lysate was clarified by centrifugation at 56,000 rpm in a Type 70 Ti rotor (Beckman Coulter) at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 60 min at 4°C (XPN-100 ultracentrifuge, Type 45 Ti rotor, Beckman, USA). The supernatant was filtered through a 0.45 μm filter (Pall ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
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bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
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bioRxiv - Bioengineering 2024Quote: ... The sample tubes were then centrifuged at 347,000 × g and 20°C for 3 hours using a Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Plant Biology 2022Quote: Protoplasts from wild type pCOBL9:GFP and cas-c1 pCOBL9:GFP transgenic plants were selected in a sorter MoFlo Astrios EQ (Beckman-Coulter), with a 70 μm nozzle at a rate of 5,000 to 10,000 events per second and a fluid pressure of 60 psi ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were filled into 38 mL open-top polypropylene tubes and subjected to DUC using a SW Type 32 Ti swinging bucket rotor (Beckman Coulter) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Neuroscience 2021Quote: ... The cleared supernatant was subsequently centrifuged at 40,000 rpm for 70 min to pellet the EVs using the type 70 Ti rotor in an Optima XPN-80 ultracentrifuge (Beckman Coulter). The supernatant resulting from this high-speed centrifugation is the EV-free ACM ...
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bioRxiv - Immunology 2020Quote: ... and the boundary layers between the two sucrose layers were harvested after ultracentrifugation (90 min, 51,000 rpm, 18 °C, Optima™ L90K centrifuge, rotor type: 70.1 Ti, tubes: 16 * 76 mm, Beckman Coulter, USA). These fractions were subsequently mixed with 6 M CsCl (AppliChem ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Microbiology 2023Quote: ... Archaella were precipitated from the supernatant at 142400 g using Beckman Coulter Opima XE-90 ultacentrifuge and the fixed-angle titanium rotor Type 45 Ti (Beckman Coulter). The tafi remained in the supernatant and could be precipitated either during many hours of high-speed centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... The UC step was an EV depletion step where the FBS was ultracentrifuged at 118,000 × g (Type 45 Ti rotor, 38,800 rpm, Beckman Coulter, Brea, CA) for 18 h at 4°C followed by a filtration step (0.22 μm) ...
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bioRxiv - Immunology 2024Quote: ... The gradient section containing assembled encapsulins was removed via aspiration and nanocontainers were isolated in a final ultracentrifugation step in a Type 70Ti rotor (Beckman Coulter) spinning at 68,000 rpm for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Density gradient ultracentrifugation using a SW Type 55 Ti swinging bucket rotor in LM-70 ultracentrifuge (Beckman Coulter, Brea, CA, USA) was achieved using OptiPrepTM iodixanol solution (60% iodixanol ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes were distributed into 6 ultra-clear thin wall tubes (Beckman Coulter Cat. 344058) and ultra-centrifuged at 50,000 G for 2 hours at 160C in a Beckman Coulter Optima XE ultracentrifuge to precipitate lentiviral particles ...
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bioRxiv - Cell Biology 2024Quote: ... 6-8 PCR reactions were pooled and purified with AMPure XP SPRI Reagent (Beckman Coulter), first at 0.6x and then at a 1x volumetric ratio ...
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bioRxiv - Microbiology 2022Quote: ... a fluorescent labeling was first made with anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution). Then ...
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bioRxiv - Immunology 2021Quote: ... The collected media were filtered with a 0.45-μm filter and ultracentrifuged at 21,000 rpm using a Type 45 Ti rotor (Beckman Coulter, Brea, CA, USA), and the pellets were resuspended in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Neuroscience 2023Quote: ... Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter, 337922). Titer was calculated using digital droplet PCR (ddPCR ...
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bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
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bioRxiv - Cell Biology 2022Quote: ... Dounced cells were centrifuged at 228 g (1,000 rpm, GH-3.8 rotor; Beckman Coulter GS-6) for 5 min at 4°C to pellet nuclei and other fragments ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with the fluorescently labeled anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution).
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bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
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bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...
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bioRxiv - Synthetic Biology 2019Quote: ... prior to CsCl density gradient ultracentrifugation (1.5 M CsCl) in Optima™ L90K centrifuge (rotor type: 70.1 Ti, tubes: 16 * 76 mm, Beckman Coulter, Brea, California, USA) for 65 h at 48,400 rpm and 4 °C ...
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bioRxiv - Genomics 2021Quote: ... Nuclei used to isolate each cell type were analyzed and sorted using a MoFlo Astrios EQ cell sorter (Beckman Coulter, Brea, CA, USA). Nuclei pellets were centrifuged for 10 min at 1500g and processed immediately for gDNA extraction using a QIAamp DNA micro kit (Qiagen ...