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Citations for Beckman :
301 - 350 of 582 citations for 8 Bromo 5 6 difluoro 2 methylquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... subsequently overlaid onto 5 mL of 20% sucrose and ultracentrifuged at 24,000 rpm for 2.5 hrs (Beckman XPN80, SW32.Ti). The viral pellet was resuspended in PBS.
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bioRxiv - Physiology 2019Quote: ... Agencourt AMPure XP PCR purification kit (5 ml Beckman Coulter Part No. A63880; 60 ml Beckman Coulter Part No. A63881) was used ...
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Proteome of the secondary plastid of Euglena gracilis reveals metabolic quirks and colourful historybioRxiv - Evolutionary Biology 2019Quote: ... The DNase-treated lysate (5 ml) was loaded on top of a sucrose discontinuous density gradient in ultracentrifuge tubes (Cat. No. 344058, Beckman). The gradient was prepared by layering decreasingly dense sucrose solutions upon one another from 2.0 ...
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bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
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bioRxiv - Immunology 2021Quote: ... 1×106 thawed PBMCs were centrifuged (750×g for 5 minutes at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrated chromatin samples were loaded on 11 mL gradients of 5-25% sucrose in TE buffer and centrifuged in a preparative ultracentrifuge with a SW41 Ti rotor (Beckman) for 11 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were centrifuged at 1500 g for 5 min in an F2402 rotor in a GS15R table-top centrifuge (Beckman) and resuspend with water to wash the cells ...
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bioRxiv - Neuroscience 2022Quote: ... trypsin-dissociated cells on 96-well plates were centrifuged at 1,000g for 5 min at 4°C using a swinging-bucket rotor for microplates (Beckman S6096) in an Allegra X-30R centrifuge (Beckman) ...
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bioRxiv - Genetics 2023Quote: ... Libraries were amplified using 5-7 cycles of PCR using KAPA HiFi DNA polymerase and purified with 1.1X SPRI (Beckman Coulter). Nucleosome profiles were manually inspected using a Tapestation with the D1000 Screen Tape system (Agilent) ...
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bioRxiv - Genomics 2023Quote: ... The suspension was incubated on ice for 5 min and centrifuged at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). The pellet was re-centrifuged for 5 min and both supernatants were combined into a new tube ...
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bioRxiv - Biophysics 2023Quote: ... Each 300μl dialysate was mixed with 300μl of 80% (w/v) Nycodenz dissolved in reconstitution buffer and then divided equally into two 5 x 41 mm ultracentrifuge tubes (Beckman). Then ...
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bioRxiv - Cell Biology 2024Quote: ... The step gradients were centrifuged for 5 h at 132.000 x g at 4 °C (SW60 Beckman and Coulter, # 335649). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were then loaded onto a 5-50% sucrose density gradient and centrifuged in an SW41 Ti rotor (Beckman) at 36,000 rpm for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysed cells were centrifuged and the viscous supernatant was immediately loaded on a 5-24% (w/v) sucrose density gradient and centrifuged in SW 32 Ti rotor (Beckman) overnight ...
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bioRxiv - Microbiology 2023Quote: Human bladder cell line PD07i was cultured at 37°C with 5% CO2 to confluency as previously described and counted using a Coulter Counter (Beckman) to a minimum of 1×105 cells/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Microbiology 2023Quote: ... Sample analysis was performed by mixing 5 µl of fluorescent vesicles with 20 µl of Flow-Count Fluorospheres (Beckman Coulter) in a final volume of 200 µl of PBS to determine absolute counts ...
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bioRxiv - Genetics 2021Quote: ... and samples were evaluated 2 minutes later with a Cytomics FC500 flow cytometer (Beckman Coulter, Brea, CA, USA) AO was excited with a 488 nm argon laser ...
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bioRxiv - Molecular Biology 2021Quote: ... Gradients were centrifuged at 36000 RPM for 2 hours at 4°C in a SW41 rotor (Beckman Coulter) and then fractions were collected using a gradient collector (Teledyne ISCO gradient station ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were purified by using 1.3X Agencourt AMPure XP beads (2 or 3 cycles; Beckman Coulter) and evaluated by using an Agilent High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: ... concentrated for 2 h by ultracentrifugation in a swing-out rotor SW-32Ti (Beckman Coulter, Brea, US-CA) at 26000 g ...
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bioRxiv - Microbiology 2020Quote: ... Purification was done by ultracentrifugation for 2 h at 154,000xg at 4°C in a SW41 rotor (Beckman) using a two-step sucrose gradient (20%/50% w/v in HNE) ...
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bioRxiv - Physiology 2019Quote: ... plasma samples were deproteinized with ZnSO4 and Ba(OH)2 and counted using a Liquid Scintillation Counter (Beckman Coulter LS6500 Multi-purpose Scintillation Counter) ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was ultracentrifuged at 47,000 g for 2 hours at 4 °C using the JA25.50 rotor (Beckman). The virus was resuspended overnight in 180 μL D10 medium at 4 °C ...
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bioRxiv - Microbiology 2019Quote: ... and adding this to 3 ml sterile inoculum water (Beckman Coulter, Inc. Brea, CA, cat no B1015-2) and 840 μl phenol red solution ...
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bioRxiv - Cell Biology 2021Quote: ... 4 °C for 2 h in an Optima L-100XP ultracentrifuge (with SW 55 Ti rotor, Beckman Coulter). Fractionation was done manually by collecting every 200 μl volume from the top till the bottom of each centrifuge tube ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were then concentrated by centrifugation for 2 hours at 20,000 rcf (Beckman Coulter, Optima L-100K) in round bottom polypropylene tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Molecular Biology 2023Quote: ... Digested extracts were then loaded on 1 M sucrose cushions 116 and centrifuged at 70,000 rpm for 2 hours at 4°C in a TLA100.4 rotor (Beckman). Ribosome pellets were resuspended in 300 µl of TRIzol reagent (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Immunology 2023Quote: ... CD27 and BA.1 or BA.2 were sorted using a MoFlo Astrios EQ Cell Sorter (Beckman Coulter). FACS data were collected by Summit 6.0 (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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bioRxiv - Neuroscience 2021Quote: ... 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge; Beckman Coulter, Fullerton, CA) for 30 min ...
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bioRxiv - Biophysics 2021Quote: ... transferred to 15 mL conical tube and centrifuged at 233 x g for 5 min at 22 °C using a swinging bucket rotor (Beckman Coulter). Pelleted cells were resuspended and washed once with 1x PBS (-CaCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... OptiPrep top to bottom density gradients 5 % to 40 % were performed as described66 in 12mL open top polyallomer tubes (Beckman Coulter). 1mL fractions were collected from the top ...
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bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
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bioRxiv - Plant Biology 2022Quote: ... diluted four times with 5 mM HEPES-KOH pH = 7.5 and centrifuged at 21,5000 rpm for 20 min (Beckman Coulter JA 25.50).
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bioRxiv - Neuroscience 2020Quote: ... A sucrose density gradient was prepared with 5 ml of both 0.8 M and 1.2 M sucrose in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). P2 homogenate was mixed with 1 ml of homogenizing buffer ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were resuspended in 5 ml SM buffer and purified via CsCl density gradient ultracentrifugation (Optima XPN-80 ultracentrifuge; Beckman Coulter) at 19,200xg for 18 h at 10°C ...
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bioRxiv - Cell Biology 2019Quote: ... with 5% fetal bovine serum (FBS) and measured on flow cytometry with well-established compensated matrix (CytoFLEX, Beckman Coulter, Pasedena, CA). Analyses were performed using CytExpert Software (Beckman Coulter).
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bioRxiv - Molecular Biology 2020Quote: ... Samples were brought to a final volume of 300 μl if necessary with lysis buffer and loaded on a non-linear sucrose gradient prepared by layering different percentage of sucrose solutions (5%, 34% and 55%) into an Open-Top Thinwall Ultra-Clear Tube (Beckman, 344059). A 5x gradient buffer (100 mM HEPES pH 7.6 ...
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bioRxiv - Biochemistry 2020Quote: ... cells were centrifuged at 4 ℃ for 5 minutes at 300g and suspended in HBSS and analyzed with a flow cytometer (CytoFLEX, Beckman Coulter). Mean fluorescence intensity was calculated for each group.
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... Insoluble proteins and cell debris were removed by centrifugation (13,000 rpm, 30 minutes at 5°C, JA-30.50Ti rotor, Beckman, Palo Alto, CA). The soluble CuMVTT CP protein in clarified lysate was pelleted using saturated ammonium sulfate (1:1 ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... 1.6 ml of 35% (w/v) sucrose and 5% (w/v) sucrose in MBS were layered in a 5.1 ml ultracentrifuge tube (Beckman Coulter, #326819) to form a discontinuous sucrose gradient ...
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bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were prepared using freeze-thaw method42 in Thinwall Ultra-Clear ultracentrifuge tubes (5 ml, 13×51 mm; Beckman-Coulter). To create the 50% sucrose cushion ...
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bioRxiv - Microbiology 2022Quote: The purified AdgI/AdgH complex sample was diluted to 0.5 mg/ml using the sample buffer containing 25 mM Tris-HCl pH 7.5 and 150 mM NaCl and subjected to the sedimentation velocity measurements using an Optima XL-I analytical ultracentrifuge (Beckman-Coulter). Data was analyzed with the SEDFIT and SEDPHAT programs33,34.