Labshake search
Citations for Beckman :
1 - 50 of 51 citations for 8 Aminoguanine 13C2 15N since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... 8 °C (Beckman Coulter Optima MAX-XP ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 8 °C (Beckman Coulter Optima MAX-XP ...
-
bioRxiv - Cancer Biology 2024Quote: ... and spinning at 12,535 g for 8 s (Beckman E centrifuge ...
-
bioRxiv - Microbiology 2023Quote: ... using a Biomek NXP Span-8 pipetting robot (Beckman Coulter), and pooled and concentrated on PCR purification columns (Analytik Jena) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
-
bioRxiv - Molecular Biology 2020Quote: ... Test PCR was cleaned with 8 μl AMPure XP beads (Beckman A63881) and eluted with 2.5 μl 0.1x TE ...
-
bioRxiv - Molecular Biology 2021Quote: ... Test PCR was cleaned with 8 μl AMPure XP beads (Beckman A63881) and eluted with 2.5 μl 0.1x TE ...
-
bioRxiv - Cancer Biology 2023Quote: ... Test PCR was cleaned with 8 μl AMPure XP beads (Beckman A63881) and eluted with 2.5 μl 0.1x TE ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 40 min at 20 000 g (8 °C, 11 300 RPM, Beckman JA-13.1) to run a 30 ml 20% Percoll purification (in 250 mM Sorbitol ...
-
bioRxiv - Immunology 2020Quote: ... in a 8 PCR cycle reaction and purified with Agencourt AMPpure XP beads (Beckman Coulter) according to the manufacturers protocol ...
-
bioRxiv - Cancer Biology 2024Quote: ... and spinning at 12,535 g for 8 s (Beckman E centrifuge, Beckman Coulter, Fullerton, CA). The tube was placed briefly in liquid nitrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... 6-8 PCR reactions were pooled and purified with AMPure XP SPRI Reagent (Beckman Coulter), first at 0.6x and then at a 1x volumetric ratio ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and diluted 1:210 into 8 new wells using a Biomek-FX pipetting robot (Beckman-Coulter). This split-pool strategy was designed to mimic the evolution conditions while maintaining sufficient diversity for bulk fitness measurements ...
-
bioRxiv - Microbiology 2021Quote: ... and centrifuged for 2 h at 64,000 g in A27-8×50 mL rotors (Beckman Coulter) to sediment virus particles ...
-
bioRxiv - Cancer Biology 2024Quote: ... Caspase 3 and 8 activity (EGFP+ cells) was analyzed using the CytoFLEX Flow Cytometer (Beckman Coulter) and Flowjo v10 BD (Becton Dickinson ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... 20 min at 20 000 g to pellet mitochondria (8 °C, 11 300 RPM, Beckman JA-13.1), 40 min at 20 000 g (8 °C ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and further centrifuged 20 min at 20 000 g (8 °C, 11 300 RPM, Beckman JA-13.1) to pellet mitochondria after Percoll removal by dilution with 40 mL mitochondrial wash buffer (250 mM Sorbitol ...
-
bioRxiv - Bioengineering 2021Quote: ... The cDNA was then subjected to 8-14 cycles of amplification before clean-up using SPRIselect Reagent (Beckman). Total cDNA yield was calculated following quality assessment (High Sensitivity D5000 ScreenTape ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... A brief centrifugation up to 4000 g (8 °C, manual stop when reaching 6000 RPM, Beckman JA-12) was used to separate chloroplast and mitochondrial fractions in pellet and supernatant respectively ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and washed in 5 volumes of chloroplast wash buffer (8 °C, manual stop when reaching 3000 RPM, Beckman JA-20 ...
-
bioRxiv - Immunology 2024Quote: ... as previously described.8 Cellularity was calculated using the Z2 Coulter Particle and Size Analyzer (Beckman Coulter, USA). For studies sorting rare populations of cells in the thymus ...
-
bioRxiv - Cell Biology 2020Quote: ... and MTs were pelleted by centrifugation at 126,000 xg for 8 minutes at 30°C in TLA100 (Beckman Coulter). Supernatant was discarded ...
-
bioRxiv - Genomics 2021Quote: ... Libraries were amplified for a total of 8-10 cycles and DNA purified using AMPure XP beads (Beckman Coulter). The quality and concentration of ATAC-seq libraries were evaluated using the Bioanalyzer DNA High Sensitivity Assay (Agilent Technologies ...
-
bioRxiv - Biochemistry 2020Quote: ... Cells were collected by centrifugation (8 000 rpm for 10 min at 4 °C, JLA-16.25 Beckman Coulter rotor), pellets frozen in liquid nitrogen and stored at −80°C ...
-
bioRxiv - Systems Biology 2021Quote: ... we pooled all 8 reactions for each sample and purified the PCR product using AMPure XP beads (Beckman Coulter). The second PCR was used to link specific indexes to each sample so we can multiplex several samples in a single sequencing run.
-
bioRxiv - Genomics 2024Quote: ... Cell concentration was measured daily (particle size 5-8 um) using a Coulter counter (Coulter Z2, Beckman Coulter GmbH), in all experimental tanks ...
-
bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 22x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 50 μL ...
-
bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 50x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl, 0.01% Tween20) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 5 μL ...
-
bioRxiv - Genomics 2021Quote: ... The combined 48 µl pools in the 8-well strip were incubated with 58 µL AMPure XP beads (Beckman Coulter) (1.2x bead to sample ratio ...
-
bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to an ultracentrifuge tube and overlayed with layers of Lysis Buffer A with 12% Ficoll and Buffer B with 8% Ficoll (AAB2209518, Fisher) and centrifuged at 28,500 rpm for 45 min (Beckman 42.1). Afterwards ...
-
bioRxiv - Neuroscience 2020Quote: ... Groups of 8 reactions were pooled to yield ∼250 μL and purified with Ampure XP Beads (0.6x ratio; Beckman Coulter), washed twice with 75% Ethanol ...
-
bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
-
bioRxiv - Genetics 2023Quote: ... Libraries were pooled up to 95-plex in equimolar amounts on a Biomek NX-8 liquid handling platform (Beckman Coulter), quantified using a High Sensitivity DNA Kit on a 2100 Bioanalyzer (Agilent) ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Populations were transferred twice a week by diluting 15% of the culture into 800 µL of fresh medium using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was prepared using Takara Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat.No.:634890) using 8 cycles of amplification and purified using Agencourt AMPure XP beads (Beckman Coulter, A63880). 1 ng of each sample cDNA was used for library preparation ...
-
bioRxiv - Biochemistry 2020Quote: ... To that was dispensed TMPRSS2 (150 nL) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give total reaction volume of 5 µL ...
-
bioRxiv - Systems Biology 2020Quote: We used two independent robot platforms with a similar basic configuration of pipettor systems (Biomek NXP automated workstations with Multichannel-96 and Span-8 pipetting model, Beckman Coulter). Additional instruments were integrated with the original workstations including incubators (Incubator Shaker DWP ...
-
bioRxiv - Neuroscience 2020Quote: ... samples with different indexes but of the same source (somata, dendrites or empty cuts) were pooled together in groups of 8 and purified using AMPure XP beads (Beckman Coulter). Libraries were further indexed ...
-
bioRxiv - Genomics 2022Quote: ... 1 µg of whole-genome amplified gDNA was sheared into ∼8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). Oxford Nanopore Technology (ONT ...
-
bioRxiv - Genomics 2022Quote: ... The non-WGA sample was used to prepare an ONT library by shearing the DNA into ~8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). The ONT library was then prepared with the Ligation Sequencing Kit (SQK-LSK109 ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatant was split into 8 fractions and exosomes were pelleted in two runs of ultracentrifugation (Optima LE-80K, Beckman Coulter) at 125,000 g for 45 min at 4 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
-
bioRxiv - Plant Biology 2024Quote: ... The index PCR was stopped after 8–10 cycles and the library DNA was purified using AMPure XP (A63880, Beckman Coulter). The library was sequenced on an Illumina NovaSeq 6000 instrument (150-bp paired-ends ...
-
bioRxiv - Microbiology 2020Quote: ... unstimulated PBMC cells (BenTech, Seattle, WA) were pelleted by centrifugation 228 x g for 8 min in an Allegra centrifuge (Beckman, Brea, CA). Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech ...
-
bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
-
bioRxiv - Neuroscience 2021Quote: ... 100 mL of each well in the 8-well strip was purified with 60 mL Ampure XP beads (0.6x ratio; Beckman Coulter, Brea, CA), washed twice with 75% ethanol ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Mitochondria were purified using a series of differential centrifugations: 20 min at 1500 g to pellet residual chloroplasts (8 °C, 3500 RPM, Beckman JA-12), 20 min at 20 000 g to pellet mitochondria (8 °C ...
-
bioRxiv - Immunology 2021Quote: ... was loaded onto the top of 6–8 M GuHCl gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 2.75 h 15°C ...
-
bioRxiv - Biochemistry 2024Quote: ... Purified nucleosome arrays were subsequently isolated on 5-40% w/v sucrose gradients in 0.5X TE by centrifuging the gradients at 39,000 rpm and 4°C for 8 h in a SW41 Ti rotor (Beckman Coulter, Brea, CA). After centrifugation ...