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Citations for Beckman :
101 - 142 of 142 citations for 8 16 Pyranthrenedione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Virus was concentrated from cell-free culture supernatant by high-speed ultracentrifugation through a 30% sucrose cushion at 80,000xg for 16 hrs in a SW32 rotor (k-factor 321) (Beckman Coulter, Brea, CA).
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bioRxiv - Biophysics 2022Quote: ... A 5 to 30% linear sucrose gradient was formed above the homogenate and centrifuged at 40,100 rpm and 4° C for 16 hours in a SW55 rotor using an OptimaTM LE-80K Ultracentrifuge (Beckman Coulter, Pasadena, CA). Fourteen 360 μl fractions were collected from the top and analyzed by SDS–PAGE/Western blot with an equal loading volume ...
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bioRxiv - Biochemistry 2020Quote: ... which was then centrifuged at 160,000×g at 4°C for 16 h in a Beckman MLS–50 rotor (Beckman–Coulter, CA, USA), and 24 fractions were collected ...
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bioRxiv - Microbiology 2021Quote: The 18S rRNA gene and 16S rRNA gene amplicons were purified with Agencourt AMPure XP beads (Beckman Coulter, Inc., Brea, CA, USA) following Illumina’s library preparation protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 20 min at 20 000 g to pellet mitochondria (8 °C, 11 300 RPM, Beckman JA-13.1), 40 min at 20 000 g (8 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and further centrifuged 20 min at 20 000 g (8 °C, 11 300 RPM, Beckman JA-13.1) to pellet mitochondria after Percoll removal by dilution with 40 mL mitochondrial wash buffer (250 mM Sorbitol ...
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bioRxiv - Synthetic Biology 2019Quote: ... prior to CsCl density gradient ultracentrifugation (1.5 M CsCl) in Optima™ L90K centrifuge (rotor type: 70.1 Ti, tubes: 16 * 76 mm, Beckman Coulter, Brea, California, USA) for 65 h at 48,400 rpm and 4 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts were prepared by incubating cells with lysis buffer for 15 min on ice followed by centrifugation for 15 min at 16,162 rcf (Beckman Coulter, Microfuge® 16). Extract concentration was quantified by bicinchoninic acid assay (Pierce #23225 ...
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bioRxiv - Cancer Biology 2021Quote: ... previously centrifuged for 16 h at 120,000 g to remove bovine exosomes using 40.1Ti rotor (L-80XP; Beckman Coulter®, Brea, CA, USA).) ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were then pooled for each of the 16S rRNA and fungal ITS1 regions after a purification/equalization process with the AMPureXP Kit (Beckman Coulter, Inc., Brea). Primer dimers ...
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bioRxiv - Biophysics 2023Quote: ... Virions were banded by density gradient centrifugation at 152,000 x g for 16 h at 4°C in a SW41 rotor (Beckman Coulter SRL, Milan, Italy). The gradient was then fractionated ...
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bioRxiv - Bioengineering 2021Quote: ... The cDNA was then subjected to 8-14 cycles of amplification before clean-up using SPRIselect Reagent (Beckman). Total cDNA yield was calculated following quality assessment (High Sensitivity D5000 ScreenTape ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A brief centrifugation up to 4000 g (8 °C, manual stop when reaching 6000 RPM, Beckman JA-12) was used to separate chloroplast and mitochondrial fractions in pellet and supernatant respectively ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and washed in 5 volumes of chloroplast wash buffer (8 °C, manual stop when reaching 3000 RPM, Beckman JA-20 ...
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bioRxiv - Molecular Biology 2024Quote: ... placed at the bottom of the tube and centrifuged at 5,000x g for 15 min at 16 °C in a swinging bucket rotor (Beckman Coulter, JS-5.3 or JS-7.5). The coverslips were recovered and fixed with pre-chilled methanol (–20 °C ...
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bioRxiv - Cell Biology 2020Quote: ... and MTs were pelleted by centrifugation at 126,000 xg for 8 minutes at 30°C in TLA100 (Beckman Coulter). Supernatant was discarded ...
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bioRxiv - Genomics 2021Quote: ... Libraries were amplified for a total of 8-10 cycles and DNA purified using AMPure XP beads (Beckman Coulter). The quality and concentration of ATAC-seq libraries were evaluated using the Bioanalyzer DNA High Sensitivity Assay (Agilent Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were collected by centrifugation (8 000 rpm for 10 min at 4 °C, JLA-16.25 Beckman Coulter rotor), pellets frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Systems Biology 2021Quote: ... we pooled all 8 reactions for each sample and purified the PCR product using AMPure XP beads (Beckman Coulter). The second PCR was used to link specific indexes to each sample so we can multiplex several samples in a single sequencing run.
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bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 22x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 50 μL ...
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bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 50x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl, 0.01% Tween20) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 5 μL ...
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bioRxiv - Genomics 2021Quote: ... The combined 48 µl pools in the 8-well strip were incubated with 58 µL AMPure XP beads (Beckman Coulter) (1.2x bead to sample ratio ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to an ultracentrifuge tube and overlayed with layers of Lysis Buffer A with 12% Ficoll and Buffer B with 8% Ficoll (AAB2209518, Fisher) and centrifuged at 28,500 rpm for 45 min (Beckman 42.1). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... Groups of 8 reactions were pooled to yield ∼250 μL and purified with Ampure XP Beads (0.6x ratio; Beckman Coulter), washed twice with 75% Ethanol ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Genetics 2023Quote: ... Libraries were pooled up to 95-plex in equimolar amounts on a Biomek NX-8 liquid handling platform (Beckman Coulter), quantified using a High Sensitivity DNA Kit on a 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were sonicated for 6-8 cycles of 20 secs and centrifuged at 40.000 rpm in MLA 80 rotor (Beckman coulter) for 45 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Populations were transferred twice a week by diluting 15% of the culture into 800 µL of fresh medium using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was prepared using Takara Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat.No.:634890) using 8 cycles of amplification and purified using Agencourt AMPure XP beads (Beckman Coulter, A63880). 1 ng of each sample cDNA was used for library preparation ...
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bioRxiv - Biochemistry 2020Quote: ... To that was dispensed TMPRSS2 (150 nL) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give total reaction volume of 5 µL ...
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bioRxiv - Systems Biology 2020Quote: We used two independent robot platforms with a similar basic configuration of pipettor systems (Biomek NXP automated workstations with Multichannel-96 and Span-8 pipetting model, Beckman Coulter). Additional instruments were integrated with the original workstations including incubators (Incubator Shaker DWP ...
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bioRxiv - Neuroscience 2020Quote: ... samples with different indexes but of the same source (somata, dendrites or empty cuts) were pooled together in groups of 8 and purified using AMPure XP beads (Beckman Coulter). Libraries were further indexed ...
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bioRxiv - Genomics 2022Quote: ... 1 µg of whole-genome amplified gDNA was sheared into ∼8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). Oxford Nanopore Technology (ONT ...
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bioRxiv - Genomics 2022Quote: ... The non-WGA sample was used to prepare an ONT library by shearing the DNA into ~8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). The ONT library was then prepared with the Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was split into 8 fractions and exosomes were pelleted in two runs of ultracentrifugation (Optima LE-80K, Beckman Coulter) at 125,000 g for 45 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... unstimulated PBMC cells (BenTech, Seattle, WA) were pelleted by centrifugation 228 x g for 8 min in an Allegra centrifuge (Beckman, Brea, CA). Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mL of each well in the 8-well strip was purified with 60 mL Ampure XP beads (0.6x ratio; Beckman Coulter, Brea, CA), washed twice with 75% ethanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Mitochondria were purified using a series of differential centrifugations: 20 min at 1500 g to pellet residual chloroplasts (8 °C, 3500 RPM, Beckman JA-12), 20 min at 20 000 g to pellet mitochondria (8 °C ...
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bioRxiv - Immunology 2021Quote: ... was loaded onto the top of 6–8 M GuHCl gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 2.75 h 15°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and serial dilutions were performed for each antibiotic using an automated culture system [34] consisting of a Biomek®NX span-8 laboratory automation workstation (Beckman Coulter, Brea, California, USA) in a clean booth connected to a microplate reader (FilterMax F5 ...