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Citations for Beckman :
101 - 150 of 467 citations for 7 9 Diazaspiro 4.5 decane 6 8 10 trione 7 ethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 6 mM β-mercaptoethanol) and spun at 43,000 rpm (214,000 × g) in a Type 45Ti rotor (Beckman) for 19 hours at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 1 h 10 min (45Ti, Beckman), resuspended in lysis buffer and stored in aliquots at –80°C until use ...
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bioRxiv - Biophysics 2020Quote: ... Cells were harvested by centrifugation for 10 min at 6000 × g (Beckman J-10; Beckman Instruments, Inc.), and the pellets were stored at −20 °C until further use.
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bioRxiv - Biophysics 2020Quote: ... Cells were harvested by centrifugation for 10 min at 6000 × g (Beckman J-10; Beckman Instruments, Inc.), and the pellets were stored at −20 °C until further use.
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation for 10 min at 5000 × g (Beckman J-10; Beckman Instruments,Inc.), and the pellets were stored at -20 °C until further use ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation for 10 min at 5000 × g (Beckman J-10; Beckman Instruments,Inc.), and the pellets were stored at -20 °C until further use ...
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bioRxiv - Bioengineering 2023Quote: ... by adding 10 μL of the PBMC solution to 10 mL of Isoton II diluent (Beckman Coulter) in a 20 mL cuvette ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Populations were transferred twice a week by diluting 15% of the culture into 800 µL of fresh medium using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was prepared using Takara Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat.No.:634890) using 8 cycles of amplification and purified using Agencourt AMPure XP beads (Beckman Coulter, A63880). 1 ng of each sample cDNA was used for library preparation ...
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bioRxiv - Biochemistry 2020Quote: ... To that was dispensed TMPRSS2 (150 nL) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give total reaction volume of 5 µL ...
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bioRxiv - Systems Biology 2020Quote: We used two independent robot platforms with a similar basic configuration of pipettor systems (Biomek NXP automated workstations with Multichannel-96 and Span-8 pipetting model, Beckman Coulter). Additional instruments were integrated with the original workstations including incubators (Incubator Shaker DWP ...
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bioRxiv - Neuroscience 2020Quote: ... samples with different indexes but of the same source (somata, dendrites or empty cuts) were pooled together in groups of 8 and purified using AMPure XP beads (Beckman Coulter). Libraries were further indexed ...
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bioRxiv - Genomics 2022Quote: ... 1 µg of whole-genome amplified gDNA was sheared into ∼8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). Oxford Nanopore Technology (ONT ...
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bioRxiv - Genomics 2022Quote: ... The non-WGA sample was used to prepare an ONT library by shearing the DNA into ~8 kb fragments followed by purification and size-selection using AMPure XP beads (Beckman Coulter). The ONT library was then prepared with the Ligation Sequencing Kit (SQK-LSK109 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was split into 8 fractions and exosomes were pelleted in two runs of ultracentrifugation (Optima LE-80K, Beckman Coulter) at 125,000 g for 45 min at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... KG, Staufen, Germany) (position 6, 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge ...
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bioRxiv - Microbiology 2021Quote: ... lysates were placed on top of a 20-50% sucrose gradient and sedimented at 35,000 rpm (218,000x g) for 6 hours at 4°C on SW40Ti rotor (Beckman). For flotation ...
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bioRxiv - Molecular Biology 2019Quote: ... and centrifuged for 6 hours at 55,000 rpm at 4°C on a TLS-55 rotor (Beckman Coulter) in a TL-100 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Neuroscience 2022Quote: ... 10-color Gallios flow cytometer (Beckman-Coulter).
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bioRxiv - Microbiology 2020Quote: ... unstimulated PBMC cells (BenTech, Seattle, WA) were pelleted by centrifugation 228 x g for 8 min in an Allegra centrifuge (Beckman, Brea, CA). Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mL of each well in the 8-well strip was purified with 60 mL Ampure XP beads (0.6x ratio; Beckman Coulter, Brea, CA), washed twice with 75% ethanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Mitochondria were purified using a series of differential centrifugations: 20 min at 1500 g to pellet residual chloroplasts (8 °C, 3500 RPM, Beckman JA-12), 20 min at 20 000 g to pellet mitochondria (8 °C ...
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bioRxiv - Immunology 2021Quote: Cells were analyzed using 4-to 6-color direct immunofluorescence staining on a Cytoflex LX or S (Beckman Coulter) flow cytometer ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were further amplified 4–6 PCR cycles as described [43] and purified using AMPure XP beads (Beckman Coulter). ATAC-seq libraries were subsequently sequenced 2 x 75 bp on an Illumina NextSeq500.
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bioRxiv - Microbiology 2020Quote: ... filtered through 45 μm-pore size filters and concentrated onto a 20% sucrose cushion by ultracentrifugation (24,000 rpm, 2h, 6°C) using a SW32 rotor (Beckman). HIV-1-based lentiviral particles were produced by co-transfecting 293T cells with packaging (psPAX2) ...
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bioRxiv - Genomics 2020Quote: ... eluted in 6 μl TE and size-selected using a 0.9X volume of Ampure XP Beads (Beckman Coulter A63882) to remove excess oligos.
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bioRxiv - Immunology 2021Quote: ... Soluble CuMV-CP-containing protein solution was separated from the cellular proteins by ultracentrifugation in a sucrose gradient (20%-60% sucrose; ultracentrifugation at 25,000 rpm for 6 hours at 5°C [SW28 rotor, Beckman]). After dialysis CuMVTT-containing gradient fractions ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was spun at 45,000 rpm (~200,000 x g) for 6 hours at 4 °C in a SW60Ti rotor (Beckman Coulter). Successive 0.4 mL fractions were collected by pipetting and their densities measured ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was centrifuged at 14,000 g for 10 min at 10°C and then loaded in an Optima analytical centrifuge (Beckman) in an An-50Ti rotor pre-equilibrated at 20°C ...
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bioRxiv - Microbiology 2021Quote: ... liquid-captured bioaerosols (5 – 10 mL) were centrifuged for 10 minutes at 3000 × g (Allegra X-15R, Beckman Coulter) and resuspended in 200 μL of Middlebrook 7H9 medium supplemented with 100 μM DMN-tre ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was cleared at 14,000g for 10 min and loaded on a linear 10-40% (w/v) glycerol gradient formed in SW32.1Ti ultracentrifugation tubes (Beckman Coulter) from the light (50 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2019Quote: ... then centrifuged at 5000 x g and 10°C for 10 minutes (Avanti□ J-E Centrifuge, Beckman Coulter, Indianapolis, IN). After disposing the supernatant ...
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bioRxiv - Developmental Biology 2023Quote: ... The top 1 mL was loaded onto a 52% isotonic Percoll solution and centrifuged at 27,000 × g for 10 min at 10°C (Optima-XPN-80, Beckman Coulter). Following centrifugation ...
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bioRxiv - Microbiology 2019Quote: ... The bacterial cells were recovered by centrifugation (in a JLA 81000 rotor, Beckman-Coulter, at 6 Krpm for 15 min) and disrupted by sonication (VibraCell 75042 sonicator ...
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bioRxiv - Microbiology 2020Quote: ... for 5 minutes using a FIBERLite F10BCI-6×500y rotor (Piramoon Technologies) in an Avanti JXN-30 centrifuge (Beckman Coulter). The clarified cell lysate was further centrifuged in a JS-24.38 rotor (Beckman Coulter ...
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bioRxiv - Biophysics 2022Quote: SpeMreBs with a volume of 200 μL were polymerised for 1 to 6 h and centrifuged (100,000 rpm, 23°C, 120 min) in a TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 200 μL of water ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged at 41399 g and 6 °C and for 1 hour (Beckman Avanti J-25 centrifuge) to obtain the cytoplasmic protein extract.
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bioRxiv - Developmental Biology 2022Quote: ... Supernatant was then filtered using 0.22 µm filter unit and divided into 6 Ultra-Clear tubs (Beckman Coulter – Ref.344058). Tubes were ultra-centrifuged at 31000 G for 1h30 at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (6 mg/ml) and floated for 2h at 55 000 rpm in a TLS-55 rotor (Beckman) at 25°C through a sucrose gradient ...
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bioRxiv - Microbiology 2022Quote: ... for 90 min or for the 6-well format at 44,000 rpm using a Beckman TLA-55 fixed angle-rotor (Beckman Coulter) for 45 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded into 6-well plates at a concentration of 4×105 cells per well and counted cumulatively with a Coulter counter (Beckman) every three days for two weeks ...
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bioRxiv - Biophysics 2023Quote: SpeMreBs in buffer S polymerized at room temperature with a volume of 200 μL were polymerized for 1–6 h and centrifuged (100,000 rpm at 23 °C for 120 min) in a TLA-100 rotor (Beckman Coulter) (2) ...
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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bioRxiv - Biochemistry 2024Quote: ... The indicated cell lines were seeded into 6 well plates and then trypsinized and cell number counted using a Z Coulter Counter (Beckman). Approximately 200 cells were seeded into 60 mm dishes and exposed 25 ...
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bioRxiv - Plant Biology 2024Quote: ... The mixture was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... The suspension was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... EVs were then placed in the bottom 36% layer of a 6-step gradient in a 13.2 ml open top polyallomer tube (331372, Beckman Coulter). A reference gradient was prepared in parallel and OptiPrep absorbance was measured at 340 nm to estimate the fraction density ...
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bioRxiv - Microbiology 2020Quote: 10-50% sucrose gradient tubes (Beckman ULTRA CLEAR) were prepared ...