Labshake search
Citations for Beckman :
51 - 100 of 1311 citations for 6 HEPTENE 2 4 DIOL 98% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
-
bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
-
bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
-
bioRxiv - Microbiology 2020Quote: ... Excess dye was removed by ultracentrifugation of the virus through a 10% sucrose cushion for 2 h at 107,000 × g and 4°C using an SW41 rotor (Beckman Coulter). Labeled virus pellets were resuspended at a viral protein concentration of 1 mg/ml ...
-
bioRxiv - Molecular Biology 2019Quote: ... for 2 h at 4°C and then further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56) for 18 h at 4°C ...
-
bioRxiv - Biophysics 2021Quote: ... The unbound cyt c was separated from liposome-bound cyt c by ultracentrifugation at 435,000 g for 2 hours at 4 °C in an Optima TLX ultracentrifuge with a TLA-100 rotor (Beckman Coulter). Immediately after the ultracentrifugation ...
-
bioRxiv - Genomics 2021Quote: ... lentiviral supernatant was ultracentrifuged at 23,000 RPM at 4 °C for 2 hours in an SW 32 Ti swinging bucket rotor (Beckman Coulter). After centrifugation ...
-
bioRxiv - Microbiology 2022Quote: Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 minutes at 4°C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 x 175 cm² flasks ...
-
bioRxiv - Cell Biology 2022Quote: ... and concentrated by 20% sucrose gradient ultracentrifugation in an Optima XPN-100 ultracentrifuge at 26,000 rpm at 4°C for 2 hours in a SW32 Ti swinging bucket rotor (Beckman-Coulter). Viral particles were resuspended in PBS and stored at −80°C.
-
bioRxiv - Microbiology 2021Quote: ... Concentrated supernatants were then subjected to ultracentrifugation at 160,000 x g for 2 hours at 4°C using a Beckman Ti 45 rotor (Beckman, Germany). Supernatants were completely removed and the pellet containing the crude MV fraction was resuspended in 400 μL of chilled PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... The gradient was subjected to high-speed centrifugation at 100,000 x g for 2 h at 4° C using a SW41Ti rotor (Beckman Coulter). 12 fractions of 1ml each were collected from the top of the gradient ...
-
bioRxiv - Neuroscience 2022Quote: ... Lentiviral supernatant was collected 48 hours after transfection and centrifuged for 2 hours by ultracentrifugation at 25,000 RPM at 4°C (Beckman Coulter). Pellets were resuspended in DPBS and frozen in -80°C until used ...
-
bioRxiv - Molecular Biology 2021Quote: ... cell lysates were cleared by centrifugation at 4 °C for 5 minutes at 16000 x g and loaded onto ice-cold 15-50 % sucrose-CHX gradients and ultracentrifuged at 4 °C for 2 hours at 274 000 x g in a SW-41Ti rotor (Beckman). Gradients were fractionated and monitored at an absorbance of 254 nm with a fraction collector (BioComp Instruments ...
-
bioRxiv - Neuroscience 2019Quote: ... The supernatant was layered onto a 20% sucrose cushion prepared in the same lysis buffer and then centrifuged at 186,000 x g for 2 h at 4 °C in a TLA-55 rotor ultracentrifuge (Beckman Coulter). RNA was isolated from the ribosome-enriched pellet using TRIzol LS reagent according to manufacturer’s instructions (Invitrogen).
-
bioRxiv - Molecular Biology 2020Quote: ... Centrifugation was conducted at 40,000 rpm for 2 hours at 4°C in a SW-41 Ti rotor (Beckman Coulter), and fractions were collected from the top of the gradient (#1 to #20) ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
-
bioRxiv - Microbiology 2022Quote: ... clarified using 0.2 µm filter and pelleted at 54,000 rpm (180,000 ×g) for 2 hours at 4 °C in a TLA-55 rotor in an Optima Max-XP tabletop ultracentrifuge (Beckman). The supernatant was removed ...
-
bioRxiv - Immunology 2022Quote: ... eVLPs were purified by ultracentrifugation at 50,000 rpm (135,000 x g) for 2 hours at 4°C using a TLA100.3 rotor and a OptimaTM TLX ultracentrifuge (Beckman Coulter) on a 20% w/v sucrose cushion ...
-
bioRxiv - Microbiology 2023Quote: ... The filtrate was ultracentrifuged at 100,000 ×g at 4 °C for 2 h using an Optima XE-90 (Beckman Coulter) to collect EMVs ...
-
bioRxiv - Cell Biology 2023Quote: ... Each fraction was subjected to a washing step in ice-cold PBS at 100,000 × g at 4°C for 2 h using a SW28Ti rotor (Beckman Coulter). The pelleted EVs were resuspended in ice-cold PBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22,000 rpm for 2 hours at 4°C with OptimaTM L-90K Ultracentrifuge (Beckman Coulter) and stored at -80°C (never refrozen).
-
bioRxiv - Plant Biology 2023Quote: ... The resulting solution was then centrifuged again at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was decanted ...
-
bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was then decanted and discarded ...
-
bioRxiv - Cancer Biology 2023Quote: ... Virus particles were collected by centrifugation at 32,000rpm for 2 hours at 4°C using the Optima XE-90 ultracentrifuge (Beckman Coulter). Virus was aliquoted and stored at -80°C until further use ...
-
bioRxiv - Cancer Biology 2023Quote: ... The gradients were ultracentrifuged at 35,000 rpm for 2 hrs at 4°C using an SW41 Ti rotor (Beckman Coulter). The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO ...
-
bioRxiv - Immunology 2023Quote: ... and purified and concentrated by ultracentrifugation on a 20% sucrose cushion (24,000 rpm and 4°C for 2 hours with a SW32Ti or SW28 rotor (Beckman Coulter)) ...
-
bioRxiv - Developmental Biology 2023Quote: ... The resulting supernatant was subjected to ultracentrifugation at 120,000 × g at 4°C for 2 h (Optima Max TL, Beckman Coulter). Pellets were resuspended in 1 ml cold PBS and centrifuged at 120,000 × g at 4°C for 2 h ...
-
bioRxiv - Cell Biology 2024Quote: ... Gradients were centrifuged at 36,000 rpm for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Following centrifugation ...
-
bioRxiv - Cell Biology 2024Quote: ... The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose) and centrifuged at 190,000 g for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Extracts were incubated with pre-washed anti-Flag beads (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: ... The samples were sedimented by velocity centrifugation at 36,000 x rpm for 2 h at 4°C using SW40Ti rotor in Optima L-80XP ultracentrifuge (Beckman Coulter). Absorbance at 254 nm was measured from lower to higher sucrose gradients using an ISCO gradient fractionation system and the optical density at 254 nm was continuously recorded with a Foxy JR Fractionator (Teledyne ISCO).
-
bioRxiv - Neuroscience 2022Quote: ... 6 colors cytometer Gallios (Beckman Coulter®). Gating strategy was determined based on the specific staining of markers for microglial population18.
-
bioRxiv - Evolutionary Biology 2021Quote: ... using a Beckman Coulter TLA100 rotor at 70,000 rpm at 4 C for 2 hours in a 13 mm x 51 mm polycarbonate ultracentrifuge tube (Beckman Coulter 349622). The sucrose solution was removed from the tube ...
-
Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 4°C at 31,000 rpm for 2 h and 30 min using an SW 32 Ti rotor(Beckman Coulter, 369694). The gradients were fractionated using a Teledyne ISCO Density Gradient Fractionation System and 12 fractions were collected ...
-
bioRxiv - Cancer Biology 2019Quote: ... Gradients were ultra-centrifuged at 221,600 × g for 2 hours at 4°C (SW-41Ti rotor, 331362, Beckman Coulter, Brea, CA). Gradients were fractionated (Piston Fractionator ...
-
bioRxiv - Immunology 2021Quote: ... Samples were spun at 76,650 x g for 2 hours at 4° C inside a SW 32i rotor using an Optima L-90K Ultracentrifuge (Beckman Coulter). Purified virus was quantified using a bicinchoninic acid assay (BCA ...
-
bioRxiv - Genomics 2021Quote: ... The samples were then centrifuged at 28,600 rpm for 2 h at 4°C (Beckman Optima XE-90; SW32 Ti rotor). After centrifugation ...
-
bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
-
bioRxiv - Neuroscience 2022Quote: ... layers F1/2 were removed before transferring the combined F3/4 layer into a clean 25mL centrifuge tube (Beckman Coulter, #355618) and diluting in GM buffer (at least 1:4 ...
-
bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
-
bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
-
bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
-
bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
-
bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
-
bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 130,000 × g for 2 h at 4°C in a TLA100.3 rotor (Beckman Optima TLX table top centrifuge). Following centrifugation ...
-
bioRxiv - Neuroscience 2021Quote: ... The post nuclear supernatant was transferred to an ultracentrifuge tube and centrifuged for 2 h at 245,000 g at 4 °C in a Beckman L90 Ultracentrifuge (Beckman Coulter, Mississauga, Canada) to pellet remaining membrane.
-
bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
-
Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Cat no UZ-PA-38.5-1) at 100,000 g at 4 °C for 2 h in a swinging-bucket rotor (Beckman Coulter; SW28 or SW32).
-
bioRxiv - Immunology 2022Quote: ... Ultracentrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2 hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, CA, USA) was performed to pellet the viruses through the sucrose cushion while soluble egg proteins were removed ...
-
bioRxiv - Microbiology 2019Quote: ... and viruses were pelleted via ultracentrifugation for 2 hours at 125,000 x g at 4°C using an SW55-Ti rotor (Beckman Coulter, Indianapolis, IN). Following centrifugation ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...