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Citations for Beckman :
51 - 100 of 112 citations for 6 Amino m ethylphenol hydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Each pool of samples was then purified using 79 μL beads buffer (20% PEG-8000 in 2.5 M NaCL, final concentrations) and 50 μL Ampure XP Beads (Beckman Coulter, A63881), and eluted in 7 μL NF H2O.
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bioRxiv - Systems Biology 2019Quote: ... were loaded on top of an 8 ml sucrose cushion (1.75 M sucrose in PEB) and centrifuged (18h, 90,000 g) using a Beckman Ti70 rotor (Beckman Coulter, Brea, USA). The resulting pellet was resuspended in 0.5 ml suspension buffer (200 mM Tris ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant were loaded on top of a discontinuous 0.75/1.0/2.33 M sucrose density gradient and centrifuge at 242,000 x g for 60 min at 4°C (Beckman SWTi41 swing rotor). The growth cone depleted fraction was collected between 0.75 M and 1.0 M sucrose ...
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bioRxiv - Cell Biology 2023Quote: ... ice-cold 5 mL 2.5 M sucrose was added to the bottom of an open-top plastic tube (Beckman-Coulter 344058), carefully topped up with ice-cold 5 mL 1.875 M sucrose ...
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bioRxiv - Molecular Biology 2022Quote: ... 6 mM β-mercaptoethanol) and spun at 43,000 rpm (214,000 × g) in a Type 45Ti rotor (Beckman) for 19 hours at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... cesium chloride continuous density gradient in resuspension buffer and centrifuged at 34,000 m for 5 hours at 20 °C using an SW41 Ti rotor (Beckman Coulter, Brea, CA). The virus particles were observed in the lower of two pale white bands in the gradient ...
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bioRxiv - Biochemistry 2019Quote: ... pH 7.4 containing 0.25 M sucrose and centrifuged at 53,000 rpm (150,000 g) in an Optima TLX ultracentrifuge (Beckman Coulter Inc., Brea, CA, USA) with a TLA100.3 rotor for 90 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... pH 7.4 containing 0.25 M sucrose and centrifuged at 53,000 rpm (150,000 g) in an Optima TLX ultracentrifuge (Beckman Coulter Inc., Brea, CA, USA) with a TLA100.3 rotor for 90 min at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Soil pH and EC were 8.3 and 12 dS m−1 that were measured by a pH meter (Beckman 45 Modal, US) and EC meter (VWR Conductivity Meter DIG2052 ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge equipped with a 50.2 Ti rotor (Beckman Coulter, Indianapolis, IN). Next ...
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bioRxiv - Neuroscience 2022Quote: ... was loaded onto a sucrose gradient made up of 5 ml 1.2 M sucrose on the bottom and 5 ml of 0.8 M sucrose at the top in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were loaded onto a 1 M sucrose cushion and then pelleted by ultracentrifugation by using a TLA100.3 rotor (Beckman Coulter, cat#349490) for 1 hour at 100,000 rpm at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... 200 μL of supernatant was loaded onto sucrose gradients (10–50% sucrose in 0.6 M KP buffer) prepared in tubes (14 × 89 mm, Open-Top Thinwall Ultraclear tubes [Beckman Coulter, CA, USA]) using a Gradient Master (Beckman Coulter) ...
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bioRxiv - Bioengineering 2023Quote: ... The supernatant was then transferred into polycarbonate tubes and centrifuged at 20,000 ×g for 45 min at 4°C to pellet down m/lEVs using an Optima XE-90 ultracentrifuge fitted with a 50.2 Ti rotor (Beckman Coulter, Indianapolis, IN). Following that ...
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bioRxiv - Synthetic Biology 2019Quote: ... prior to CsCl density gradient ultracentrifugation (1.5 M CsCl) in Optima™ L90K centrifuge (rotor type: 70.1 Ti, tubes: 16 * 76 mm, Beckman Coulter, Brea, California, USA) for 65 h at 48,400 rpm and 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 M sucrose in HK was added to the reaction to a final concentration of 1 M (“Input” sample) and transferred to 7 × 20 mm polycarbonate centrifuge tubes (Beckman Coulter cat #343775). HK buffer with 0.75 M sucrose was gently laid on top of the reaction mixture followed by a layer of HK ...
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bioRxiv - Neuroscience 2021Quote: ... KG, Staufen, Germany) (position 6, 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge ...
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bioRxiv - Microbiology 2021Quote: ... lysates were placed on top of a 20-50% sucrose gradient and sedimented at 35,000 rpm (218,000x g) for 6 hours at 4°C on SW40Ti rotor (Beckman). For flotation ...
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bioRxiv - Molecular Biology 2019Quote: ... and centrifuged for 6 hours at 55,000 rpm at 4°C on a TLS-55 rotor (Beckman Coulter) in a TL-100 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Physiology 2019Quote: ... Samples were then centrifuged at 100,000 xG at 4 °C for 20 hours in a swinging bucket rotor (Beckman L8-M Ultracentrifuge, SW28 Rotor).
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bioRxiv - Immunology 2021Quote: Cells were analyzed using 4-to 6-color direct immunofluorescence staining on a Cytoflex LX or S (Beckman Coulter) flow cytometer ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were further amplified 4–6 PCR cycles as described [43] and purified using AMPure XP beads (Beckman Coulter). ATAC-seq libraries were subsequently sequenced 2 x 75 bp on an Illumina NextSeq500.
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bioRxiv - Microbiology 2020Quote: ... filtered through 45 μm-pore size filters and concentrated onto a 20% sucrose cushion by ultracentrifugation (24,000 rpm, 2h, 6°C) using a SW32 rotor (Beckman). HIV-1-based lentiviral particles were produced by co-transfecting 293T cells with packaging (psPAX2) ...
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bioRxiv - Genomics 2020Quote: ... eluted in 6 μl TE and size-selected using a 0.9X volume of Ampure XP Beads (Beckman Coulter A63882) to remove excess oligos.
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bioRxiv - Immunology 2021Quote: ... Soluble CuMV-CP-containing protein solution was separated from the cellular proteins by ultracentrifugation in a sucrose gradient (20%-60% sucrose; ultracentrifugation at 25,000 rpm for 6 hours at 5°C [SW28 rotor, Beckman]). After dialysis CuMVTT-containing gradient fractions ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was spun at 45,000 rpm (~200,000 x g) for 6 hours at 4 °C in a SW60Ti rotor (Beckman Coulter). Successive 0.4 mL fractions were collected by pipetting and their densities measured ...
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bioRxiv - Biochemistry 2024Quote: ... NaCl was then added to 1 M and the lysed solution was centrifuged at 20,000 g at 1°C for 45 minutes (Beckman Coulter J2-21, JA 20 rotor). Tween-20 was added to the supernatant to 0.1% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... The bacterial cells were recovered by centrifugation (in a JLA 81000 rotor, Beckman-Coulter, at 6 Krpm for 15 min) and disrupted by sonication (VibraCell 75042 sonicator ...
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bioRxiv - Microbiology 2020Quote: ... for 5 minutes using a FIBERLite F10BCI-6×500y rotor (Piramoon Technologies) in an Avanti JXN-30 centrifuge (Beckman Coulter). The clarified cell lysate was further centrifuged in a JS-24.38 rotor (Beckman Coulter ...
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bioRxiv - Biophysics 2022Quote: SpeMreBs with a volume of 200 μL were polymerised for 1 to 6 h and centrifuged (100,000 rpm, 23°C, 120 min) in a TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 200 μL of water ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged at 41399 g and 6 °C and for 1 hour (Beckman Avanti J-25 centrifuge) to obtain the cytoplasmic protein extract.
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bioRxiv - Developmental Biology 2022Quote: ... Supernatant was then filtered using 0.22 µm filter unit and divided into 6 Ultra-Clear tubs (Beckman Coulter – Ref.344058). Tubes were ultra-centrifuged at 31000 G for 1h30 at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (6 mg/ml) and floated for 2h at 55 000 rpm in a TLS-55 rotor (Beckman) at 25°C through a sucrose gradient ...
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bioRxiv - Microbiology 2022Quote: ... for 90 min or for the 6-well format at 44,000 rpm using a Beckman TLA-55 fixed angle-rotor (Beckman Coulter) for 45 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded into 6-well plates at a concentration of 4×105 cells per well and counted cumulatively with a Coulter counter (Beckman) every three days for two weeks ...
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bioRxiv - Biophysics 2023Quote: SpeMreBs in buffer S polymerized at room temperature with a volume of 200 μL were polymerized for 1–6 h and centrifuged (100,000 rpm at 23 °C for 120 min) in a TLA-100 rotor (Beckman Coulter) (2) ...
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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bioRxiv - Biochemistry 2024Quote: ... The indicated cell lines were seeded into 6 well plates and then trypsinized and cell number counted using a Z Coulter Counter (Beckman). Approximately 200 cells were seeded into 60 mm dishes and exposed 25 ...
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bioRxiv - Plant Biology 2024Quote: ... The mixture was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... The suspension was then centrifuged for 20 min at 9,700 g at 6°C using an Avanti J-E centrifuge and a JLA-16.250 rotor (Beckman Coulter, USA). The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... EVs were then placed in the bottom 36% layer of a 6-step gradient in a 13.2 ml open top polyallomer tube (331372, Beckman Coulter). A reference gradient was prepared in parallel and OptiPrep absorbance was measured at 340 nm to estimate the fraction density ...
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bioRxiv - Biochemistry 2024Quote: ... The cells were sonicated for 6-8 cycles of 20 secs and centrifuged at 40.000 rpm in MLA 80 rotor (Beckman coulter) for 45 min ...
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bioRxiv - Neuroscience 2020Quote: ... The pH-insensitivity of the Ag/AgCl pellet was confirmed via measurements against a double junction Ag/AgCl reference electrode (Beckman Coulter, 3.5 M KCl, Item #A57189). We found the pH sensitivity of our custom-made EIROF deposited μRC electrodes to be 70 mV/log-decade at body temperature of 37°C (Fig ...
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bioRxiv - Microbiology 2020Quote: ... were added into cells (1×106 cells/sample in 100 μL) and incubated for 60 min at 2-6°C and analyzed using flow cytometry (CytoFLEX, Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown for 4-6 days and counted using an Incucyte (Essen Biosciences) or a Z2 Coulter Counter (Beckman Coulter). Small molecule dose-response curves were printed using an D300e Digital Dispenser (HP Specialty Printing Systems ...
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bioRxiv - Cell Biology 2022Quote: ... Resulting libraries were then pooled in sets of 6 (approximately 36,000 beads per pool) and products purified by Agencourt AMPure XP beads (Beckman Coulter, A63881) with a 0.6x volume wash followed by a 0.8x volume wash ...
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bioRxiv - Microbiology 2022Quote: ... The recovered supernatant of both induced and uninduced cultures was transferred in 6 polyallomer (36 ml each) centrifuge tubes (25×89 mm, Beckman Coulter) to which 1 ml of Protease Inhibitor Cocktail (Sigma Aldrich ...