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Citations for Beckman :
1 - 50 of 434 citations for 5 Methylsulfamoylmethyl 1H indole 3 carboxylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 and 5 and counted using a particle counter (Z1; Beckman Coulter, Inc.). Experiments were carried out in triplicate ...
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bioRxiv - Biochemistry 2023Quote: ... followed by centrifugation at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14) in order to remove cell debris ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Cell Biology 2020Quote: ... Both subfractions were centrifuged in a Ti50 rotor at 225,000g for 1h (Beckman Type 70.1Ti rotor) at 4C ...
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bioRxiv - Biochemistry 2023Quote: ... followed by lysate centrifugation at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14). The supernatant was incubated for 45min ...
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bioRxiv - Biochemistry 2023Quote: ... the lysate was centrifuged at ∼18000g for 1h at 4°C (Beckman Coulter centrifuge, JA-20) and the supernatant incubated for 45min ...
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bioRxiv - Biochemistry 2022Quote: ... Insoluble material was removed by ultracentrifugation at 158,000 ×g for 1h (Beckman L-90K, Ti70 rotor), and membranes were filtered with a 0.45 μm syringe filter and applied to a 0.5 mL anti-FLAG M2 affinity gel in a column (Millipore Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was then centrifuged at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14) and the supernatant incubated for 1h ...
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bioRxiv - Neuroscience 2022Quote: ... Insoluble material was removed by ultracentrifugation (1h, 4°C, 40,000 rpm, Ti70 rotor, Beckman Coulter Cat#337922). The fragments were immobilized onto glutathione Sepharose (Millipore/Cytiva Cat#17-5132-02 ...
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bioRxiv - Biochemistry 2023Quote: ... After ultracentrifugation for 1h at 258,488 x g at 4°C using a TLS-55 rotor (Beckman), the fractions were analysed by SDS-PAGE and the positive fractions were concentrated ...
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bioRxiv - Microbiology 2020Quote: ... 1mM dithiothreitol and 100μg/ml cycloheximide) and spun for 1h at 100K RPM using TLA-110 rotor (Beckman) at 4c ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by spinning at 3,000xg followed by 20,000xg for 10min each and supernatant were loaded on a 30% sucrose cushion and spun for 1h at 31,200 r.p.m in SW60 rotor (Beckman Coulter). The non-polysomal supernatant was removed and polysomes collected below the sucrose layer was diluted with the hypotonic buffer [10mM HEPES pH 7.8 ...
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bioRxiv - Microbiology 2020Quote: ... 1mM dithiothreitol and 100μg/ml cycloheximide) and spun for 1h at 100K RPM using TLA-110 rotor (Beckman) at 4c ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Microbiology 2020Quote: ... the 100,000xg centrifugation was performed for 1h at 4°C using a SW41 TI swinging bucket rotor (Beckman Coulter). The pellet was then resuspended in 50 μL or 1500 μL of D-MEM for subsequent analysis.
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bioRxiv - Cell Biology 2020Quote: ... Fractions were diluted and washed in PBS for 1h at 100,000 x g in a TLA 110 rotor (Beckman). Fractions were resuspended in 35 ul of PBS ...
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bioRxiv - Biophysics 2021Quote: ... 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
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bioRxiv - Developmental Biology 2023Quote: ... the supernatant was centrifuged in a TLA100.4 fixed angle rotor at 100000 g for 1h at 37°C in an Optima TL ultracentrifuge (Beckman). The supernatant containing globular actin was collected ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was washed with PBS and ultracentrifuged at 100,000 × g 1h at 4°C to obtain small vesicles (TL-100 Ultracentrifuge, Beckman Coulter). The final pellet was resuspended in PBS for subsequent experiments ...
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bioRxiv - Biochemistry 2020Quote: ... the crude mitochondria were undergone a sucrose gradient in SEM buffer for 1h at 139,400 x g with an SW40 Ti rotor (Beckman Coulter). Mitochondrial band was pooled and stored at −80 °C.
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bioRxiv - Microbiology 2021Quote: ... The membrane fraction was prepared using ultracentrifugation (45,000 rpm, 4 °C, 1h; Optima-90 ultracentrifuge, Beckman-Coulter, Brea, CA, USA) and washed once in 200 mM ABC buffer ...
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bioRxiv - Microbiology 2021Quote: ... The resting supernatants were filtered through 0.45µm syringe filters and ultracentrifuged at 100,000 x g for 1h at 4°C (SW41 Ti swinging-bucket rotor, Beckman Coulter). The supernatant was discarded and the pellet suspended in 0.22 µm-pore filtered or 0.02 µm-pore filtered (for Flow Cytometry analysis ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter, 369650; Optima XE, Beckman Coulter). Phagosome-containing beads float at the interface between the 10% and 25% layers ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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bioRxiv - Molecular Biology 2024Quote: ... bottom layer was made by diluting OptiPrep™ solution with clarified HM or digesta sample (2.25 mL OptiPrep™ + 0.75 mL sample = 3 mL) into a 5 mL open-top thin wall ultra-clear tube (Beckman Coulter). A discontinuous gradient was made with 40% (w/v) ...
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bioRxiv - Pathology 2024Quote: ... with primers located in the U3 region of the 5’ and 3’ LTRs (Figure S1) and purified using “1X AmpureXP beads” (Beckman Coulter). ONT (Oxford nanopore technologies ...
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bioRxiv - Microbiology 2022Quote: ... and the viral particles were concentrated by ultracentrifugation for 1h at 30,000 rpm at 4°C in an SW40 rotor (Beckman, Fullerton, CA). Viral pellets were resuspended in TNC buffer (10 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatants were collected and subjected to high-speed centrifugation at 100,000 × g for 1h (TLS-55 rotor, OptimaTMMAX Ultracentrifuge; Beckman Coulter, Germany). The supernatants (S100 ...
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bioRxiv - Plant Biology 2023Quote: ... The disrupted cells were subjected to ultracentrifugation at 4°C and 235000 g for 1h (Beckman Optima™ L-80 XP, rotor Ti45). The resulting supernatant was loaded onto an Äkta purifier system (GE HealthCare ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and supernatants were ultracentrifuged at 37 000 rpm (~100 000 g) and 10°C for 1h (Beckman Optima LE-80 K; rotor 70.1.Ti). Pellets were resuspended in 100μL of buffer (10 mM Tris-HCL ...
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bioRxiv - Biophysics 2021Quote: ... Following incubation (37 °C, 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
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bioRxiv - Biochemistry 2021Quote: ... The UV-Visible spectrum of each sample was recorded for both tannic acid and gallic acid for both pH’s using a DU730 UV-Visible Spectrometer (Beckman Coulter, Pasadena, CA). The spectra were recorded from 250-400 nm with 0.2 nm data intervals ...
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bioRxiv - Immunology 2024Quote: ... After purification of nucleic acids with SPRIselect (Beckman Coulter) and subsequent treatment with RNase inhibitor consisting of DW (Nacalai ...
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bioRxiv - Genomics 2022Quote: ... Nucleic acid purification was performed using SPRI Select (BECKMAN COULTER), nucleic acid concentration was determined by Qubit High Sensitivity DNA assay (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... After purification of nucleic acids by Agencourt RNA Clean XP (Beckman Coulter) and subsequent treatment with DNase I ...
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bioRxiv - Immunology 2020Quote: ... KIR2DL2/3/S2 (GL183, PE/Cy5.5, Beckman Coulter,), NKG2C (134591 ...
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bioRxiv - Systems Biology 2023Quote: ... enumerated using a Multisizer 3 Coulter Counter (Beckman,), and then seeded at the desired density in 6-well tissue culture plates (TPP ...
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bioRxiv - Genetics 2022Quote: ... 5 mL (Beckman Coulter, B23317) using a DynaMag™-2 Magnet magnetic stand (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
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bioRxiv - Molecular Biology 2021Quote: ... triglycerides and non-esterified fatty acids using a serum analyzer (AU480 Chemistry analyzer Beckman Coulter). Plasma levels of leptin and insulin were assessed using ELISAs used according to the manufacturer’s instructions (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... nucleic acids were extracted from sorted single cell using RNAClean XP SPRI beads (Beckman Coulter), and RNA was hybridized first using RT primer (/5BiosG/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TVN ...
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bioRxiv - Developmental Biology 2020Quote: ... a CytoFLEX 3 laser 13 colour flow cytometer (Beckman Coulter) was used ...