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Citations for Beckman :
1 - 50 of 431 citations for 3 3 17 17 Bis ethylenedioxy 19 hydroxyandrost 5 ene 19 d2 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... After a 19 h centrifugation at 70,000g (SW32.1 rotor, Beckman), the gradients were fractionated ...
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bioRxiv - Neuroscience 2022Quote: ... 5 ml of this mix was transferred to a 17-ml ultracentrifuge tube (Beckman) and overlayed with the following sucrose solutions ...
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bioRxiv - Cell Biology 2023Quote: ... After centrifugation at 13,000 rpm (Beckman, JA-17) for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... Tie2-PE and CD34-FITC antibodies (eBioscience 17-5821-81, 17-1171, 12-5987, 25-0411, 11-0341-82) by flow cytometry (Beckman Coulter CyAn ADP) or confocal microscopy using Confocal Microscope Zeiss LSM 880 with Airyscan.
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bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
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bioRxiv - Microbiology 2020Quote: ... the virus particles were pelleted by ultracentrifugation in a Beckman type 19 rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... After centrifugation at 235,000g for 19 h at 4 °C in a 45Ti rotor (Beckman), the supernatant was dialyzed against buffer A without spermine ...
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bioRxiv - Immunology 2021Quote: ... Samples from COVID-19 patients and controls were assessed on a Cytoflex LX cytometer (Beckman Coulter), verified through daily calibration with CytoFLEX Daily QC Fluorospheres (Beckman Coulter) ...
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bioRxiv - Biophysics 2022Quote: ... and the crude extract was centrifuged at 39,000 xg (JA-17 rotor, Beckman-Coulter) for 30 min at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... and the crude extract was centrifuged at 17000 g (JA-17 rotor, Beckman-Coulter) for 30 min at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Immunology 2024Quote: ... Insoluble cellular debris was removed by centrifugation in a JA-17 rotor (Beckman Coulter) at 14,000 rpm for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 50%) in a 17 mL ultracentrifuge tube (Ultra-Clear, 16×102 mm, 344061, Beckman Coulter), and leaving overnight to allow for merging of the layers into a continuous gradient ...
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bioRxiv - Genetics 2024Quote: ... The filtered medium was centrifuged at 19400 rpm and 17 °C for 2 h (Beckman Coulter). The pellet was suspended in 250 µL HBSS (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 minutes at 4°C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 x 175 cm² flasks ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Ribosomes were pelleted by centrifugation at 63,000g for 16–19 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). For all ultracentrifugation steps indicated here ...
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bioRxiv - Microbiology 2022Quote: ... at 20 KPSI at 4°C and then centrifuged at 19 000 RPM in a JA-25.50 rotor (Beckman Coulter) for 30 minutes at 4°C ...
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bioRxiv - Biochemistry 2020Quote: The cell lysates obtained after sonication were centrifuged (16000 rpm, JA-17, 45 min, 4 °C, Beckman) to remove unlysed cells and other high molecular weight debris ...
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bioRxiv - Biochemistry 2020Quote: ... 20 mM Tris:HCl pH 7.5) in a zonal rotor (Ti 15, Beckman, 17 hours at 21,000 rpm). The peak containing pure 70S ribosomes was pelleted by centrifugation (20 hours at 35,000 rpm) ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged through a 10–35% sucrose gradient for 19 h at 16,000 rpm in a SW28 rotor (Beckman Coulter, Inc.). Fractions containing the 70S peaks were pooled and kept at −80℃ for further use.
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 49,100g for 16 to 17 hrs at 4°C in an SW32Ti rotor (Beckman). Gradients were fractionated using a Brandel (UV ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 and 5 and counted using a particle counter (Z1; Beckman Coulter, Inc.). Experiments were carried out in triplicate ...
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bioRxiv - Biophysics 2020Quote: ... 150 μl of the sample was ultracentrifuged at 22°C and 205000 × g for 19 min (TLA 100.3, Beckman Coulter Inc., Fullerton, California). The experiments were performed in HEPES buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 17 cycles were used for cDNA pre-amplification followed by cDNA clean-up with SPRIselect reagent (B23318, Beckman Coulter) and cDNA quantification ...
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bioRxiv - Microbiology 2020Quote: ... The gradient was centrifuged for 17 h at 4°C and 100,000 rcf (23,700 rpm) using an SW 40 Ti rotor (Beckman Coulter), followed by manual fractionation into 20 590 μl fractions and measurement of the A260 nm of each fraction ...
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bioRxiv - Cell Biology 2022Quote: ... 200 μL of extract was loaded on the 11 mL 5–20% sucrose gradient in the XB buffer and subjected to ultracentrifugation for 17 h at 197,000 ×g in the swinging bucket rotor SW41 Ti (Beckman). 0.5 mL fractions were collected and concentrated by using trichloroacetic acid precipitation with insulin as a carrier ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: CsCl linear gradients were prepared by overlaying 8.5 mL of 1.46 g/cm3 CsCl solution with 8.5 mL of 1.2 g/cm3 CsCl solution in 17 mL Ultra-Clear tubes (Beckman Coulter), which were then spun at a 45-degree angle and a speed of 20 rpm for 13.5 minutes using Gradient Master (BioComp).
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bioRxiv - Systems Biology 2023Quote: ... Gradients were centrifuged at 4°C for 17 h at 100,000 g using an L7 ultracentrifuge with an SW28 swing out rotor (Beckman Coulter). Fractions (F ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter; OptimaTM L-90K, Beckman Coulter). After the ultracentrifugation ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting solution was then centrifuged again at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was decanted ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was then decanted and discarded ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixture was applied onto the top of the gradient solution and was centrifuged at 41,000 rpm at 4 °C for 17 hrs using an SW41 Ti rotor (Beckman Coulter). The gradient was fractionated using a Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Microbiology 2024Quote: ... and fractionated by centrifugation at 32 000 rpm (130 000 × g) for 17 h using an SW-41 rotor (Beckman). The gradient was divided into 20 fractions and proteins from each fraction were resolved on SDS-PAGE and detected by western blotting.
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bioRxiv - Microbiology 2024Quote: ... 1 mM MgCl2) and fractionated by centrifugation at 130 000 × g for 17 h using an SW-41 rotor (Beckman). The gradient was divided into 20 fractions and proteins from each fraction were resolved on SDS-PAGE gels and detected by western blotting.
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bioRxiv - Molecular Biology 2021Quote: ... mtEFG1 was subsequently removed from the mitoribosomes via a 10 – 40 % (w/v) sucrose gradient in 1x MB (17 h, 22500 rpm, SW 32 Ti rotor (Beckman Coulter), 4 °C) ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (16) ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (18) ...
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bioRxiv - Microbiology 2024Quote: ... The top agar was then scraped off the LB agar and centrifuged for 26,000 x g at 4°C for 1 hour (Beckman Coulter JA-17). The supernatant was then removed ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants were collected and purified through 15-45% (w/v) sucrose density gradients by ultracentrifugation at 151,000 x g (using 17 mL tubes in a Beckman SW32.1 Ti rotor) for 3 h at 10°C21 ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Immunology 2020Quote: ... KIR2DL2/3/S2 (GL183, PE/Cy5.5, Beckman Coulter,), NKG2C (134591 ...
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bioRxiv - Systems Biology 2023Quote: ... enumerated using a Multisizer 3 Coulter Counter (Beckman,), and then seeded at the desired density in 6-well tissue culture plates (TPP ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...