Labshake search
Citations for Beckman :
451 - 500 of 885 citations for 3' 3' Cyclic GAMP cGAMP ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... Resuspended pellets were then subjected to an iodixanol density gradient in 5-mL Ultra-Clear centrifuge tubes (Beckman Coulter). The tubes were first filled with a two-layer gradient ...
-
bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (5 mg/ml) and floated for 2h at 55,000 rpm in a TLS-55 rotor (Beckman) at 25 °C through a sucrose gradient ...
-
bioRxiv - Cell Biology 2022Quote: ... The stock solution of aggregate-depleted BSA (5% w/v) was prepared by overnight centrifugation at 100,000 x g (SW28 rotor Beckman Coulter ...
-
bioRxiv - Cell Biology 2022Quote: ... and 3.5 mL of 5% iodixanol in a 16.8 mL open top polyallomer tube (Beckman Coulter, Fullerton, California, USA). One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter ...
-
bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed by centrifugation at 1,300 g for 5 min (Beckman Centrifuge Avanti® J-26-XP). Afterwards ...
-
bioRxiv - Neuroscience 2023Quote: ... after which the cells were harvested by centrifugation at 4,000 rpm for 30 min at 5°C using the JLA 8.1000 rotor (Beckman Coulter). The harvested pellets were stored at −20°C until use ...
-
bioRxiv - Microbiology 2022Quote: ... the stained cells were detached by 5 mM of EDTA/PBS and analyzed with a CytoFLEX Flow Cytometer (Beckman). 10,000 events in a gated live cell population (based on SSC/FSC ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cell lysates were subjected to two rounds of centrifugation at 18000 rpm for 5 and 30 mins in a JA-25.50 rotor (Beckman). Protein levels were normalized for coimmunoprecipitation and immunoblot analysis using a Bradford Assay ...
-
bioRxiv - Cancer Biology 2023Quote: ... Percentage of cells with each DNA content (2N, 3N, 4N, 5-8N) were estimated using CytExpert software (Beckman Coulter). For DNA content analysis using sorted cells ...
-
bioRxiv - Microbiology 2024Quote: ... respectively) were gently layered on top of each other in ultracentrifuge tubes (Ultra-Clear 5 mL Tube, Beckman Coulter) and a continuous gradient was generated using a gradient maker (Gradient Master ...
-
bioRxiv - Biophysics 2024Quote: ... Each seed mix was clarified using an ultra-centrifuge (Beckman Optima MAX-XP, 90,000 RPM, 4°C, 5 min) then snap-frozen in small aliquots and stored at -80°C ...
-
bioRxiv - Microbiology 2024Quote: ... The phage was purified by isopycnic centrifugation in a CsCl discontinuous gradient for 5 h at 32,000 rpm in a SW41 rotor (Beckman) at 20°C ...
-
bioRxiv - Cancer Biology 2019Quote: ... A quantity of 10 μl of lentiviral particles was added to the appropriate wells and the cells and lentiviral particles containing plates were immediately centrifuged at 32°C for 90 minutes at 2500 rpm speed in a centrifuge (Beckman Allegra X-12R). After spinoculation ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 20 μL reactions were prepared by dispensing 1 μL of each 10 μM reverse primer into the wells of a 96-well PCR plate using the Echo liquid handler (Beckman Coulter, Brea, CA). A mastermix consisting of polymerase premix ...
-
bioRxiv - Biochemistry 2021Quote: ... was arrayed in a minimum of a triplicate across a 10 kDa MWCO 96-well microdialysis plate (SWISSCI Diaplate™) and sealed with aluminum foil seals (Beckman Coulter 538619) to create the protein chambers ...
-
bioRxiv - Cell Biology 2021Quote: ... The fluorescence generated by the reduction of resazurin to resorufin by live cells was measured using a Beckman coulter DTX 880 multimode plate reader (Beckman Coulter, Brea, California) (570/600 nm).
-
bioRxiv - Pathology 2022Quote: ... Emission from Renilla Luciferase and Citrine was measured simultaneously at 465 nm and 535 nm in a plate-reader (Beckman coulter, Villepinte, France). EPAC1 activation decreases BRET signal (465 nm/535 nm ratio).
-
bioRxiv - Evolutionary Biology 2023Quote: ... Five thousand cells from each of the 300 samples were analyzed in 96-well plates in a CytoFLEX Platform flow cytometer (Beckman Coulter, California, USA) at the Feldan Therapeutics facility (Quebec ...
-
bioRxiv - Pathology 2020Quote: ... 200 μl were added to LBF lysis buffer (supplied with the kit) and viral RNA was extracted using RNAdvance Viral Kit (Beckman Coulter) and further processed on the Biomek i7 Automated Workstation (Beckman Coulter) ...
-
bioRxiv - Immunology 2022Quote: ... and total RNA was isolated using Isospin cell and tissue RNA kit (Nippon Gene) or an RNAdvance v2 kit (Beckman Coulter) according to manufacturer instructions and quantified with an RNA HS Assay Kit (Thermo Fisher ...
-
bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2020Quote: ... Four mL of each density (3.5 mL for the 5% density) was stacked into an open polyallomer tube (337986, Beckman Coulter) using a Tecan freedom EVO ...
-
bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... 5 × 105 additional cells were immediately processed for far red signal analysis by flow cytometry (Gallios, Beckman Coulter, Brea, USA) (D0) ...
-
bioRxiv - Genomics 2019Quote: ... DNA samples were loaded onto 5-20% sucrose gradients and fractionated according to size by centrifugation (SW-40Ti rotor; Beckman Coulter Optima L-100 XP ...
-
bioRxiv - Microbiology 2019Quote: ... Then 0.8 mL of Cold 60% sucrose buffer A was added to RNAse-free 5 mL Ultraclean tubes for ultracentrifugation in a SW55Ti (Beckman). The ribosome-containing supernatant was used to fill these tubes and an ultracentrifugation step was performed for 16 hs at 150.000g ...
-
bioRxiv - Microbiology 2020Quote: ... for 5 minutes using a FIBERLite F10BCI-6×500y rotor (Piramoon Technologies) in an Avanti JXN-30 centrifuge (Beckman Coulter). The clarified cell lysate was further centrifuged in a JS-24.38 rotor (Beckman Coulter ...
-
bioRxiv - Microbiology 2020Quote: ... At the end of each selection cells were pelleted from the media by centrifugation 200 x g for 5 min in an Allegra centrifuge (Beckman) and supernatant was transferred for storage at −80C ...
-
bioRxiv - Microbiology 2020Quote: MT-2 cells were collected and washed three times by pelleting at 200 x g for 5 min at 4°C in an Allegra centrifuge (Beckman) and resuspending cells in ice-cold PBS ...
-
bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
-
bioRxiv - Cell Biology 2022Quote: ... brought up to 3 ml using sterile 20 mM Tris-HCl pH 7.5 and pelleted at 40,000g and 4°C for 60 minutes (TLA100.3 rotor, Optima Max-XP ultracentrifuge, Beckman Coulter, Inc.). Finally ...
-
bioRxiv - Immunology 2022Quote: ... 1.5 μl of PI was also added and samples were run on the Gallios™ Flow Cytometer (Beckman Coulter, Inc). Data was analysed offline using Kaluza® Flow Analysis software version 2.1a (Beckman Coulter Inc. ...
-
bioRxiv - Molecular Biology 2020Quote: ... The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K, Beckman Coulter). The pellet was then resuspended in the required volume of PBS before being aliquoted and stored at −70°C.
-
bioRxiv - Immunology 2021Quote: ... subsequently overlaid onto 5 mL of 20% sucrose and ultracentrifuged at 24,000 rpm for 2.5 hrs (Beckman XPN80, SW32.Ti). The viral pellet was resuspended in PBS.
-
Proteome of the secondary plastid of Euglena gracilis reveals metabolic quirks and colourful historybioRxiv - Evolutionary Biology 2019Quote: ... The DNase-treated lysate (5 ml) was loaded on top of a sucrose discontinuous density gradient in ultracentrifuge tubes (Cat. No. 344058, Beckman). The gradient was prepared by layering decreasingly dense sucrose solutions upon one another from 2.0 ...
-
bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
-
bioRxiv - Immunology 2021Quote: ... 1×106 thawed PBMCs were centrifuged (750×g for 5 minutes at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
-
bioRxiv - Molecular Biology 2022Quote: ... Concentrated chromatin samples were loaded on 11 mL gradients of 5-25% sucrose in TE buffer and centrifuged in a preparative ultracentrifuge with a SW41 Ti rotor (Beckman) for 11 hrs ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were centrifuged at 1500 g for 5 min in an F2402 rotor in a GS15R table-top centrifuge (Beckman) and resuspend with water to wash the cells ...
-
bioRxiv - Genetics 2023Quote: ... Libraries were amplified using 5-7 cycles of PCR using KAPA HiFi DNA polymerase and purified with 1.1X SPRI (Beckman Coulter). Nucleosome profiles were manually inspected using a Tapestation with the D1000 Screen Tape system (Agilent) ...
-
bioRxiv - Genomics 2023Quote: ... The suspension was incubated on ice for 5 min and centrifuged at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). The pellet was re-centrifuged for 5 min and both supernatants were combined into a new tube ...
-
bioRxiv - Cell Biology 2023Quote: ... 5 mM Mg(OAc)2) and centrifuged for 20 min at 100,000 x g in a 70Ti rotor (Beckman Coulter). The resulting membrane pellet was resuspended in 1 ml rough microsome (RM ...
-
bioRxiv - Biophysics 2023Quote: ... Each 300μl dialysate was mixed with 300μl of 80% (w/v) Nycodenz dissolved in reconstitution buffer and then divided equally into two 5 x 41 mm ultracentrifuge tubes (Beckman). Then ...
-
bioRxiv - Cell Biology 2024Quote: ... The step gradients were centrifuged for 5 h at 132.000 x g at 4 °C (SW60 Beckman and Coulter, # 335649). Subsequently ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysates were then loaded onto a 5-50% sucrose density gradient and centrifuged in an SW41 Ti rotor (Beckman) at 36,000 rpm for 2 h at 4 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Lysed cells were centrifuged and the viscous supernatant was immediately loaded on a 5-24% (w/v) sucrose density gradient and centrifuged in SW 32 Ti rotor (Beckman) overnight ...
-
bioRxiv - Microbiology 2023Quote: Human bladder cell line PD07i was cultured at 37°C with 5% CO2 to confluency as previously described and counted using a Coulter Counter (Beckman) to a minimum of 1×105 cells/mL ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... Sample analysis was performed by mixing 5 µl of fluorescent vesicles with 20 µl of Flow-Count Fluorospheres (Beckman Coulter) in a final volume of 200 µl of PBS to determine absolute counts ...
-
bioRxiv - Cancer Biology 2023Quote: ... we pooled the contents of all the wells of a 384-well plate together and purified the resulting library using AMPure XP beads (Beckman Coulter, cat. no. A63881) at 1.8 v/v ratio ...