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Citations for Beckman :
1 - 50 of 1314 citations for 2 6 Di 1 tert butyl 1 1 diphenylsilyl oxy 9 10 dihydroanthracene 9 10 dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The ratio of Ca2+-bound Indo-1 versus Ca2+-free Indo-1 was analyzed using FlowJo 9 software (Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... 1 h 10 min (45Ti, Beckman), resuspended in lysis buffer and stored in aliquots at –80°C until use ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded onto 10-45% CHX supplemented sucrose gradients in 9/16 x 3.5 PA tubes (Beckman Coulter, #331372) and spun at 35,000 x g in SW41 for 3 hours at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were loaded onto 10-45% CHX supplemented sucrose gradients in 9/16 x 3.5 PA tubes (Beckman Coulter, #331372) and spun at 35,000 x g in an SW41 rotor for 3 hours at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (J33, Beckman Coulter; 1:10) or CD3-APC ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (UCHT1; Beckman Coulter; 1:10) were used to stain T-cells in co-culture assays ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Molecular Biology 2020Quote: ... diluted in 9 mL of 10 mM Tris-HCl buffer and centrifuged for 45 min at 45.000 rpm (Beckman 70.1 Ti Rotor). Pellets were suspended in the same buffer and analyzed by SDS-PAGE using 12.5% polyacrylamide gel ...
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bioRxiv - Cancer Biology 2021Quote: ... Dead cells were excluded using the viability dye 7AAD (1:10; Beckman Coulter, A07704). Compensation was performed using mouse IgG CompBeads (BD Biosciences ...
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bioRxiv - Cancer Biology 2021Quote: ... They were then diluted 1:10 in Coulter Isoton II diluent (Beckman Coulter, 8546719) and cell diameters determined using a particle sizing and counting analyser (Multisizer 4 Coulter counter ...
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bioRxiv - Cell Biology 2021Quote: ... About 9 ml interface that came from 3×SW28 tubes (25×89 mm, Beckman Coulter) was loaded in a SW41 tube (14×89 mm ...
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bioRxiv - Genomics 2022Quote: ... We cleaned the reaction using 10 μl (1 x ratio) AMPure XP beads (Beckman Coulter) and eluted the reaction in 20 μl UltraPure DNase/RNase free ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
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bioRxiv - Genomics 2020Quote: PCR products for each sample were pooled by targeted gene in equimolar ratios then each pool was 1:10 diluted and subsequently cleaned with 1 volume of AMPure XP (Beckman Coulter, Brea, California, USA) before quantification ...
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bioRxiv - Immunology 2021Quote: ... Flow cytometry was performed on CyAN ADP LX 9-colour flow cytometer (Beckman Coulter, Pasadena, CA).
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bioRxiv - Immunology 2021Quote: Flow cytometry was performed on CyAN ADP LX 9-colour flow cytometer (Beckman Coulter, Pasadena, CA) or LSRII (Becton Dickinson) ...
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bioRxiv - Microbiology 2021Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 μL of sterile water ...
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bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
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bioRxiv - Biochemistry 2021Quote: ... using 1-10 ng DNA and libraries were size selected with AMPure XP beads (Beckman Coulter). Illumina 50 bp single-end read sequencing was performed on a NextSeq 500 or NovaSeq 6000 ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... PCR amplicons for pools 1 and 2 were combined for each sample and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30μL of sterile water ...
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bioRxiv - Biochemistry 2021Quote: ... 9 s relapse) and centrifuged twice at 11,000 x g for 20 min at 4°C (Beckman) to pellet cell debris ...
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bioRxiv - Molecular Biology 2022Quote: ... Purification of cDNA was performed manually using 9 µl of AMPure XP bead suspension (A63880, Beckman Coulter) per well according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mL samples were supplemented with 500 uL of Optiprep to yield a 20% iodixanol solution and underlayered beneath 1.5 mL layers of 9% and 12% Optiprep/PBS solutions in a 4.9 mL OptiSeal tube (Beckman-Coulter, 362185) using a disposable glass cannula ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Illumina 16S Metagenomic Sequencing Library Preparation Protocol with the following changes: 10 μL of Amplicon product was purified using a 1:0.8 ratio of XP beads (Beckman Coulter), with beads resuspended in a final volume of 10 μL of nuclease-free water ...
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bioRxiv - Molecular Biology 2020Quote: ... The library was amplified with 9 PCR cycles and cleaned with 0.9x AMPure® XP beads (A63881, Beckman Coulter, CA, USA) to enrich for DNA fragments shorter than 50nt ...
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bioRxiv - Immunology 2023Quote: Koff rates were determined as dissociation of reversible pMHC-Streptamers upon addition of D-Biotin in a flow cytometry-based assay (CyAn ADP Lx 9 color flow cytometer, Beckman Coulter) as described previously (44) ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Synthetic Biology 2021Quote: ... The complete NanoTrap was purified using rate-zonal centrifugation33 through a 1545% glycerol gradient in 1×TE + 10 mM MgCl2 in an SW 55 rotor (Beckman Coulter). Fractions were collected after a 1 hr centrifugation at 50 k rpm ...
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Microbiology 2023Quote: ... 1 μl or 10 μL of dilution were added to the 384-well plates using automated dispenser BioMek FX (Beckman Coulter). Extracellular bacteria were then eliminated by adding antibiotic to the media 1h after infection ...
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Plant Biology 2024Quote: ... The pre-concentrated culture was centrifuged (10000 g, 10 min) in 1-L buckets (Avanti J-265 XP, Beckman Coulter, USA). The biomass reached a concentration of approximately 40 % (dry weight - DW ...
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bioRxiv - Biochemistry 2024Quote: ... Digested pellets were loaded onto a 10-50% sucrose gradient (sucrose dissolved in 1× lysis buffer) and ultracentrifuged using a SW41 Ti rotor (Beckman Coulter) at 40,000 rpm (273,620 × g ...
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Inhibition of Ribosome Biogenesis in vivo Causes p53-Dependent Death and p53-Independent DysfunctionbioRxiv - Cell Biology 2024Quote: ... 1 mL of the lysates was loaded onto a 10%-50% sucrose gradient in a 14 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a Beckman Optima LE-80K Ultracentrifuge Floor Centrifuge at 261,000 × g for 135 minutes at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... EV–containing fractions were pooled (1.10–1.18 g/mL) and diluted by adding 9 ml PBS to SW40 tubes (Beckman–Coulter, Fullerton, CA, USA). EVs were pelleted at 200,000xg for 60 min at 4°C (Beckman–Coulter Optima™ L–90K or Optima™ XPN–80 centrifuge ...
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bioRxiv - Cell Biology 2020Quote: ... A 1:1 Ampure XP (Beckman Coulter) PCR purification step was performed followed by assessment of size distribution using an Agilent high-sensitivity DNA chip (Agilent technologies ...
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were permeabilized for intracellular staining via resuspension in 0.5% Triton X-100 in PBS for 10 min and were subsequently resuspended in a 1:500 dilution of anti-p24-FITC KC57 antibody (Beckman Coulter #6604665) in 1% BSA in PBS for 1 h at room temperature in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
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bioRxiv - Genetics 2024Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean Ampure XP beads (Beckman Coulter, A63987).
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometry was performed using a FACSort or FACScalibur flow cytometer (Becton Dickinson, Mountain View, CA) and a CyAN ADP LX 9-colour flow cytometer (Beckman Coulter, Pasadena, CA). Data were analyzed using FlowJo software (Tree Star Inc ...
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bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
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bioRxiv - Immunology 2024Quote: ... PD-1 (PE-Cy5.5, 1/320, Beckman Coulter). Staining was performed in 1X PBS with 5% FBS and incubated in the dark for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... PD-1 (PE-Cy5.5, 1/320, Beckman Coulter). Staining was performed in 1X PBS with 5% FBS and incubated in the dark for 30 minutes ...
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bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
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bioRxiv - Cancer Biology 2020Quote: ... CD14 BV605 (1:100) and CD11b PC5 (1:100, Beckman Coulter). Cells were analyzed on the FACSCelesta (BD).
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bioRxiv - Systems Biology 2022Quote: ... and purification with Ampure XP beads (1:1 ratio, Beckman Coulter) produced a purified cDNA library ...