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Citations for Beckman :
1 - 50 of 1365 citations for 1 3 Nitro 10 11 dihydro 5H dibenzo b f azepin 5 yl ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and the cleared supernatant was loaded on a 3 to 30% iodixanol gradient (OptiPrep) and ultracentrifuged for 5h at 36,000 rpm in an SW60 rotor (Beckman Coulter). Fractions were collected manually through aspiration and RNA or proteins were isolated for further analysis.
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bioRxiv - Systems Biology 2020Quote: ... in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778), and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2 ...
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bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Linear 10–30% glycerol gradients were prepared in 11□mm□×□34□mm polyallomer centrifuge tubes (Beckman Coulter, Brea, CA) with HEM buffer [25 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2020Quote: ... and layered onto a continuous 10-50 w/w % sucrose gradient prepared in a 2.2 ml ultra-clear tube (11×34 mm, Beckman Coulter) using a two-step program in Gradient Master 108 machine ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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bioRxiv - Immunology 2023Quote: ... Measurements were performed in a Gallios 10/3 cytometer (Beckman Coulter), using the Kaluza for Gallios Acquisition software (Version 1.0 ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 and 5 and counted using a particle counter (Z1; Beckman Coulter, Inc.). Experiments were carried out in triplicate ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Immunology 2021Quote: ... Samples were measured on a Gallios (10 colors, 3 lasers, Beckman Coulter) flow cytometer using FACS Kaluza software v2.1.1 (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2020Quote: ... at 50.000 rpm and 11 °C (Beckman SW65 Ti Rotor). The virion band was extracted using an 18‐ gauge needle syringe ...
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bioRxiv - Cancer Biology 2022Quote: ... a 0.55× volume (11 µL) of SPRIselect (Beckman Coulter, B23317) was added to the purified library ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 (SAN4024, Sanplatec, Osaka, Japan) in a 10 ml tube (361707, Beckman Coulter). The 2K fraction was placed on the top or bottom of the media after dissolving the pellet in 500 µl of 8% or 48% iodixanol in 0.02 M HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
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bioRxiv - Cell Biology 2022Quote: ... and the pellet was resuspended with 5 ml buffer B and transferred into a 14 ml ultracentrifuge tube (331372, Beckman Coulter). 9 ml Buffer C (1.8 M sucrose ...
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bioRxiv - Cell Biology 2020Quote: Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Microbiology 2021Quote: ... liquid-captured bioaerosols (5 – 10 mL) were centrifuged for 10 minutes at 3000 × g (Allegra X-15R, Beckman Coulter) and resuspended in 200 μL of Middlebrook 7H9 medium supplemented with 100 μM DMN-tre ...
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bioRxiv - Biophysics 2020Quote: ... in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman, ref. 343788). Ribosome pellets were washed once and subsequently resuspended with ribosome storage buffer (30 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Biochemistry 2021Quote: ... 1 h 10 min (45Ti, Beckman), resuspended in lysis buffer and stored in aliquots at –80°C until use ...
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bioRxiv - Biophysics 2022Quote: ... Labeled G-actin was clarified to remove any remaining F-actin or precipitated FMAL by centrifugation (10 min, 90,000 rpm in a Beckman TLA-120.2 rotor at 4°C). The extent of labeling (typically around 70% ...
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bioRxiv - Biophysics 2024Quote: ... and LD_KMP-11_KO /KMP-11 complemented cultures using CytoFLEX Flow Sorter (Beckman Coulter) in a 15ml tube and MΦ infection was performed as described previously39 ...
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bioRxiv - Neuroscience 2022Quote: ... anti-CD90 (IM1839U / Beckman Coulter / F15-42-1-5), anti-CD146 (A07483 / Beckman Coulter / TEA1-34) ...
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bioRxiv - Microbiology 2022Quote: Chlorophyll (a+b) was extracted from 1 ml of algal or algal-bacterial pellets with ethanol (1 ml) and measured spectrophotometrically (Beckman Coulter, DU 800) (Wintermans and De Mots ...
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bioRxiv - Immunology 2020Quote: ... a lyse-wash protocol (Optilyse B, Beckman-Coulter) was performed ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 40 min at 20 000 g (8 °C, 11 300 RPM, Beckman JA-13.1) to run a 30 ml 20% Percoll purification (in 250 mM Sorbitol ...
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bioRxiv - Cell Biology 2023Quote: ... The PMF was loaded in two 11 × 60 mm centrifuge tubes (cat. 344062, Beckman) and then centrifuged on an SW 60 Ti rotor (Beckman ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (J33, Beckman Coulter; 1:10) or CD3-APC ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (UCHT1; Beckman Coulter; 1:10) were used to stain T-cells in co-culture assays ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Immunology 2021Quote: ... Purified cDNA was amplified for 11 cycles before being cleaned up using SPRIselect beads (Beckman). Samples were diluted 4:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5) in a 11 x 34 mm Ultra-Clear centrifuge tubes (Beckman Coulter: 347356). The sucrose density gradient was centrifuged at 50,000 rpm for 3 hours at 4°C in a TLS-55 swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... CD103 (APC, B-Ly7, eBioscience, or biotin, 2G5, Beckman Coulter ...
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bioRxiv - Immunology 2024Quote: ... and DuraClone IM B cell tubes (#B53318, Beckman Coulter) respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... Beckman Coulter TUBES UC 1 X 3 ½ (cat# NC9146666) was purchased from Beckman. Flotillin-1 antibody (cat# NBP2-75492 ...
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bioRxiv - Microbiology 2022Quote: ... phage suspension was layered on top of a 5–20 % sucrose (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) and centrifuged (Beckman Optima L-K90 ...
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bioRxiv - Microbiology 2022Quote: ... The light-scattering zone was collected and further purified in 20– 70 % sucrose gradient (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) by centrifugation (Beckman Optima L-K90 ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... followed by sequential topping with 2 mL each of 35, 25, and 10% sucrose (all prepared in 3 mM imidazole, pH 7.4) in SW40 tubes (344060, Beckman). The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman) ...
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bioRxiv - Molecular Biology 2024Quote: ... bottom layer was made by diluting OptiPrep™ solution with clarified HM or digesta sample (2.25 mL OptiPrep™ + 0.75 mL sample = 3 mL) into a 5 mL open-top thin wall ultra-clear tube (Beckman Coulter). A discontinuous gradient was made with 40% (w/v) ...
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bioRxiv - Pathology 2024Quote: ... with primers located in the U3 region of the 5’ and 3’ LTRs (Figure S1) and purified using “1X AmpureXP beads” (Beckman Coulter). ONT (Oxford nanopore technologies ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of the eluate was transferred into a Beckman 11-mm ultracentrifuge tube (Beckman Coulter) for ultracentrifugation in an Optima TLX ultracentrifuge (Beckman Coulter ...
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bioRxiv - Immunology 2024Quote: ... Purified cDNA was amplified for 11 cycles before being cleaned up using SPRIselect beads (Beckman Coulter). Samples were diluted 9:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were amplified for 11 PCR cycles and size selected using SPRI selected beads (Beckman Coulter).
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II ...
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bioRxiv - Immunology 2023Quote: ... and B cells were determined using Flow-Count fluorospheres (Beckman) and a Cytoflex flow cytometer (Beckman ...
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bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...