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Citations for Beckman :
401 - 450 of 1814 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Bone marrow cell numbers and viability were determined using an automated cell counter (ViCell Blu, Beckman Coulter). Spleen cell suspensions were generated by crushing whole spleen and filtering through a 70 μM filter ...
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bioRxiv - Cancer Biology 2021Quote: ... Total cell counts were determined from purified mononuclear cell suspensions using a DxH500 Hematology Analyzer (Beckman Coulter). Purified cell suspensions were stained for 30 - 40 minutes at 4°C in the dark with a master mix of the respective antibodies followed by a washing step in PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell numbers were counted using either a Hemocytometer or the ViCell Cell Viability Analyzer (Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... Cells were sorted on a MoFlo Astrios high speed cell sorting device (Beckman-Coulter, Brea, CA, USA), and only DAPI-negative cells ...
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bioRxiv - Cell Biology 2020Quote: ... Resistant cells were then single cell sorted into 96-well plates using a MoFlo Astrios (Beckman Coulter) to begin clonal cell lines.
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bioRxiv - Immunology 2020Quote: Single cell RNA was purified from individual B cells using magnetic beads (Beckman Counter, RNAClean XP, A63987) and used for cDNA synthesis by reverse transcription (SuperScript III Reverse Transcriptase ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-positive C20 microglia were sorted as single cells using the MoFlo XDP Cell Sorter (Beckman-Coulter). Successful ATM KO clones were identified by fragment analysis using capillary electrophoresis (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... cell numbers and cell volumes were determined using Z2 Coulter particle count and size analyzer (Beckman Coulter) to calculate total cell volume for each sample ...
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bioRxiv - Immunology 2022Quote: ... Cell numbers of each cell subset were evaluated by using Flow-Count Fluorospheres (Beckman Coulter, Brea, CA) and presented as numbers per tissue after exclusion of doublet and dead cells.
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bioRxiv - Cell Biology 2023Quote: ... and cells with the same fluorescence intensities were collected by a cell sorter (MoFlo AstriosEQ; Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... 10% of the sorted cells were used to measure cell size using a Z2 Coulter counter (Beckman), and the rest of the cells were lysed in RIPA lysis buffer supplemented with protease inhibitors and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2023Quote: ... Multiple-organelle marker co-expressing cells were isolated using a cell sorter (MoFlo Astrios EQ, Beckman Coulter).
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bioRxiv - Neuroscience 2023Quote: ... cells were dissociated and sorted using the C.SIGHT clonal dispenser (Cytena) or the MoFlo cell sorter (Beckman) in 96-well plates ...
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bioRxiv - Plant Biology 2021Quote: ... Cell density and mean cell diameter were measured using a Coulter Counter (Multisizer 3, Beckman Counter, Brea, CA). For the data in Figure 1A ...
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bioRxiv - Cell Biology 2020Quote: ... and cell size and cell density were measured using the Multisizer 4 Coulter Counter (Beckman-Coulter, Mississauga, ON) or collected for whole cell lysis at time points indicated in the figures ...
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bioRxiv - Cancer Biology 2022Quote: ... transduced cells were selected using 1μg/mL puromycin (LabForce) or sorted on an Astrios cell sorter (Beckman Coulter) dependent on the vector.
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bioRxiv - Developmental Biology 2022Quote: ... CD31+TdTomato+ cells underwent FACS-based isolation from single cell suspension using a MoFlo Astrios EQ (Beckman Coulter) flow cytometer with antibody staining for CD31-PECy7 (25-311-82 ...
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bioRxiv - Cancer Biology 2019Quote: ... GFP-negative cancer cells were sorted using the MoFlo XDP cell sorter with a 100μm nozzle (Beckman Coulter) as part of the University of Colorado Cancer Center Flow Cytometry Shared Resource ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Then the cells were sorted into empty 96-well plates using MoFlo Astrios EQ cell sorter (Beckman Coulter). Gates were set mainly based on Mitotracker intensity and the dye was detected by a 488 nm and 640 nm laser for excitation ...
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bioRxiv - Immunology 2020Quote: ... Unstimulated cells and pokeweed mitogen-stimulated cells were additionally stained with AF750 anti-CD19 (Beckman Coulter, clone J3119). Samples were incubated 15 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: Cells were analysed by flow cytometry for GFP expression or cell surface staining using Gallios (Beckman Coulter, UK), Attune (Thermo ...
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bioRxiv - Immunology 2023Quote: ... White blood cell counts were determined from full blood with an automatic cell counter (DxH 500, Beckman Coulter). For bioluminescent imaging ...
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bioRxiv - Plant Biology 2023Quote: ... Cell density and mean cell volume were measured using a Coulter Counter (Multisizer 3, Beckman Counter, Brea, CA). For 35°C with photoautotrophic medium ...
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bioRxiv - Systems Biology 2023Quote: ... and then taking cell density measurements (cells/mL) with a coulter counter (Beckman Coulter Z2 Particle Counter #6605700) every 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Excess dye was removed by ultracentrifugation of the virus through a 10% sucrose cushion for 2 h at 107,000 × g and 4°C using an SW41 rotor (Beckman Coulter). Labeled virus pellets were resuspended at a viral protein concentration of 1 mg/ml ...
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bioRxiv - Genomics 2020Quote: ... RAD library quality was inspected on a 2% agarose gel before undergoing a final AMPure XP (Beckman Coulter, Indianapolis, IN) purification and quantification on a Qubit 2.0 Fluorometer (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... The crude nuclei were stained with 4,6-diamidino-2-phenylindole and loaded into a flow cytometer (Beckman Coulter MoFlo XDP). Nuclei were purified by flow sorting and washed in accordance with (70) ...
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bioRxiv - Molecular Biology 2019Quote: ... for 2 h at 4°C and then further purified using CsCl equilibrium gradient ultracentrifugation at 35,000 rpm (Beckman SW56) for 18 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... Sucrose gradient ultracentrifugation was performed for 2 hours and 45 minutes at 35,000 rpm in a SW 41 Ti rotor (Beckman-Coulter) at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... with 2 ml of 20% sucrose in dPBS cushion and ultracentrifugated at 20,000 RPM (Beckman Coulter SW 32 Ti rotor) at 4°C for 2 hours ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ug/ml cycloheximide) and macromolecular complexes were separated by velocity gradient ultracentrifugation (SW41Ti – Beckman, 39,000 rpm, 2 hr, 4oC). Fractions were collected using Biocomp Gradient Station and pooled to obtain separate monosomal and polysomal fractions ...
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bioRxiv - Biophysics 2021Quote: ... The unbound cyt c was separated from liposome-bound cyt c by ultracentrifugation at 435,000 g for 2 hours at 4 °C in an Optima TLX ultracentrifuge with a TLA-100 rotor (Beckman Coulter). Immediately after the ultracentrifugation ...
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bioRxiv - Cell Biology 2021Quote: ... Collected FT were then subjected to ultracentrifugation for 2 hours at 200 000g using the TLA 45 rotor (Beckman Coulter) and resuspended in 20μL of Laemmli 1X (BioRad) ...
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bioRxiv - Biochemistry 2021Quote: ... Equal parts of 30% and 10% iodixanol gradient solutions were layered on the mitochondrial fraction and then samples were centrifuged at 50,000 x g for 2 h (Beckman SW41TI). The mitochondrial layer was collected ...
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bioRxiv - Cell Biology 2021Quote: Microtubules were sedimented onto poly-L-lysine coated coverslips by centrifuging through 20% glycerol BRB80 cushion at 4700 rpm for 2 h in S6096 rotor (Beckman). Coverslips were subsequently fixed with 20°C methanol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The collected supernatants were centrifuged at 300,000 x g for 2 hr with Optima MAX-XP ultracentrifuge with SW 55 Ti Swinging-Bucket Rotor (Beckman Coulter). The pellets consisting of the cell membrane fractions were resuspended into 10 mL of the same glycan containing media and incubated in the anaerobic chamber at 37°C for 16 hr ...
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bioRxiv - Microbiology 2021Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 μL of sterile water ...
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bioRxiv - Genomics 2021Quote: ... lentiviral supernatant was ultracentrifuged at 23,000 RPM at 4 °C for 2 hours in an SW 32 Ti swinging bucket rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and centrifuged at 100,000 × g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Microbiology 2022Quote: Extracellular HSV-1 or HSV-2 particles were harvested by sedimentation at 12,000 rpm for 90 minutes at 4°C (Type 19 rotor, Beckman-Coulter) from the medium of BHK-21 cells (40 x 175 cm² flasks ...
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bioRxiv - Cell Biology 2022Quote: ... and concentrated by 20% sucrose gradient ultracentrifugation in an Optima XPN-100 ultracentrifuge at 26,000 rpm at 4°C for 2 hours in a SW32 Ti swinging bucket rotor (Beckman-Coulter). Viral particles were resuspended in PBS and stored at −80°C.
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bioRxiv - Developmental Biology 2020Quote: ... Virus-containing supernatant was collected for two consecutive days and concentrated by ultracentrifugation for 2 hours at 19,400 rpm using an Optima L-80 XP Ultracentrifuge (Beckman Coulter).
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bioRxiv - Cancer Biology 2020Quote: ... samples were suspended in FACS buffer (PBS, 20 mM EDTA pH 8.0, 2% FCS) and acquired using a CyAn ADP9 flow cytometer (Beckman Coulter), FACS LSRII Fortessa or FACSymphony (both BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... pspAX.2 and oligonucleotides were incubated for 48 h and GFP expression level was analyzed by CytoFLEX flow cytometer (Beckman).
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bioRxiv - Microbiology 2021Quote: ... Concentrated supernatants were then subjected to ultracentrifugation at 160,000 x g for 2 hours at 4°C using a Beckman Ti 45 rotor (Beckman, Germany). Supernatants were completely removed and the pellet containing the crude MV fraction was resuspended in 400 μL of chilled PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The gradient was subjected to high-speed centrifugation at 100,000 x g for 2 h at 4° C using a SW41Ti rotor (Beckman Coulter). 12 fractions of 1ml each were collected from the top of the gradient ...
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bioRxiv - Neuroscience 2022Quote: ... Lentiviral supernatant was collected 48 hours after transfection and centrifuged for 2 hours by ultracentrifugation at 25,000 RPM at 4°C (Beckman Coulter). Pellets were resuspended in DPBS and frozen in -80°C until used ...
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bioRxiv - Neuroscience 2022Quote: ... new medium was added for another round of production and supernatant was filtered (0.45 μm filter) and centrifuged for 2 hours at 19400 rpm (SW28 Beckman rotor). The pellets were suspended in phosphate buffer saline (PBS) ...
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bioRxiv - Microbiology 2022Quote: ... was added before ultracentrifugation at 20,000 × g for 2 hours at 10°C in an Optima L-90K Ultracentrifuge with the SW32Ti rotor (Beckman Coulter). Supernatants were carefully discarded by aspiration and the 6 phage pellets were resuspended with 0.1 ml of TM buffer (50 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2021Quote: Reactions were assembled in a final volume of 2 μL using a Labcyte Echo® 550 (Beckman Coulter, Brea, CA) via a two-step strategy ...