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Citations for Beckman :
401 - 450 of 1034 citations for 1 3 Fluorophenyl 5 oxopyrrolidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1).
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bioRxiv - Neuroscience 2021Quote: ... Before FACsorting we added the 5 ul of the cellular death dye Draq7TM (Viability dye, Far-red DNA intercalating agent, Beckman Coulter, #B25595). The suspension was sorted on an Astrios II cell sorter (Beckam Coulter) ...
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bioRxiv - Plant Biology 2021Quote: ... cesium chloride continuous density gradient in resuspension buffer and centrifuged at 34,000 m for 5 hours at 20 °C using an SW41 Ti rotor (Beckman Coulter, Brea, CA). The virus particles were observed in the lower of two pale white bands in the gradient ...
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bioRxiv - Immunology 2021Quote: ... were loaded onto 5-25% (w/v) linear sucrose gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 3 h 15°C ...
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bioRxiv - Genomics 2021Quote: ... with 5 PCR reactions per column then pooled together and cleaned up a second time with 1.6X SPRI beads (Beckman Coulter, Brea, California). The CHEQ-seq vector containing a random 17 bp BC upstream of the synthetic intron was linearised via inverse PCR with primers “CHEQseq_lin_A_For” and “CHEQseq_lin_A_Rev” or “CHEQseq_lin_B_For” and “CHEQseq_lin_B_Rev” ...
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bioRxiv - Neuroscience 2022Quote: ... was loaded onto a sucrose gradient made up of 5 ml 1.2 M sucrose on the bottom and 5 ml of 0.8 M sucrose at the top in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor ...
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bioRxiv - Cell Biology 2024Quote: ... and cells were isolated by centrifugation at 500 g for 5 min at 4°C followed by being treated with a red blood lysis buffer (Beckman Coulter, USA). After washing with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with 5 μg/ml PI (ST1569-10mg, Beyotime) and immediately analyzed using the CytoFLEX flow cytometer (Beckman Coulter, USA). Tumor cells were distinguished based on GFP positivity or CD45 negativity ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned media were centrifuged at 1000 rpm for 5 min to remove cell debris and then either added to Ultra-Clear Quick-Seal ultracentrifuge tubes (Beckman Coulter 246.344322), 100 kDa molecular weight cut-off centrifugal filters (MWCO ...
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bioRxiv - Plant Biology 2024Quote: ... The cell lysate was centrifuged at about 16000 g for 10 min at 5 °C (Avanti J-265 XP, Beckman Coulter, USA). The starch pellet was recovered ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 nL/well of library compounds were transferred to the columns 5-48 of each plate by using Echo 525 acoustic dispenser (Beckman Coulter, CA). The microplates were incubated at room temperature for 10 minutes and then loaded on the FLIPR Penta High-Throughput Cellular Screening System (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... and reactions were cleaned with Agencourt AMPure XP beads (1:1 volume; Beckman Coulter, A63881) retaining first-strand cDNA ...
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bioRxiv - Cancer Biology 2020Quote: ... The libraries were purified in a 1:1 volumetric ratio of Ampure beads (Beckman Coulter), Pippin size selection was performed between 200-1000bp (Sage Science) ...
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bioRxiv - Cell Biology 2021Quote: 1 mL samples of culture were diluted 1:100 with Isoton II Diluent (Beckman Coulter) and measured on a Beckman Multisizer 3 Coulter Counter to produce a histogram of the population’s cell volumes ...
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bioRxiv - Cancer Biology 2020Quote: ... Excess adapter was removed using a 1:1 volumetric ratio of Ampure beads (Beckman Coulter). Bisulfite conversion was performed using the EZ-DNA methylation kit (Zymo ...
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bioRxiv - Neuroscience 2023Quote: ... The scWGA products were then cleaned with AMPure XP beads (Beckman Coulter; 1:1 ratio).
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bioRxiv - Genomics 2021Quote: ... The spores were washed twice with sterile ddH2O and pelleted at 3700g for 5 min at 4 °C in an Allegra X-12R centrifuge (Beckman, Brea, CA, USA). Spores were resuspended in sterile ddH2O and kept at 4°C until experiments.
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bioRxiv - Biophysics 2021Quote: ... Different ratios of LUVs and cyt c (with the protein concentration kept at 5 μM) were incubated in the sample buffer at room temperature for 15 min in ultracentrifugal tubes (Beckman Coulter, Indianapolis, IN). The effective PG/cyt c molar ratio (R ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected and centrifuged for 5 min at 1200 rpm at 20°C (Allegra™ X-12R Centrifuge, Beckman Coulter, the Netherlands). Cells were then resuspended in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were pelleted and washed twice before resuspension in 500μL of cold PBS for analysis using flow cytometry (CytoFLEX 5, Beckman Coulter, Mississauga, ON, Canada) wherein mean fluorescence intensity of the gated cells (10,000 events ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We pooled 1.5 µl of each indexed library (including blanks and museum controls) and performed size selection with AMPure XP beads (Beckman Coulter, IN, USA) for fragments of approximately 100-500 bp in length ...
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bioRxiv - Microbiology 2022Quote: ... then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter, Indianapolis IN, USA) to remove large debris ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was collected and treated with polyethyleneimine to a final concentration of 0.03% incubated on ice for 5 minutes and subsequently centrifuged at 10,000 rpm at 4°C for 10 minutes (Beckman coulter, JA-25.50 rotor). The supernatant was collected and powdered ammonium sulfate (approx 15 g ...
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bioRxiv - Cell Biology 2024Quote: Lung nonparenchymal cells isolated from WT mice with and without LPS treatment (n=5/group) were stained as previously described (18) and analyzed using a CytoFlex (Beckman Coulter, Brea, CA). Dead cells were excluded using Live/Dead Near IR (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: 1 mL samples of culture were diluted 1:100 diluted with Isoton II Diluent (Beckman Coulter) and used to determine cell concentration of the culture using a Beckman Multisizer 3 Coulter Counter ...
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bioRxiv - Genomics 2022Quote: ... The DNA fragments were purified by Ampure XP beads (volume ratio 1:1, Beckman Coulter, #A63881) and elute the DNA fragments in 27 µL water ...
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bioRxiv - Cancer Biology 2023Quote: ... Post-PCR clean-up was performed using 1:1 volume Ampure XP beads (Beckman Coulter A63880). Samples were dissolved in 10 mM Tris-HCl (pH 8 ...
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bioRxiv - Microbiology 2024Quote: ... DNA was purified using a 1:1 ratio of sample to AMPure XP Beads (Beckman Coulter), and eluted into EB buffer ...
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bioRxiv - Cell Biology 2024Quote: ... 1:20 human FITC-BRIC6 (IBGRL Research) and 1:20 human APC-anti-CD49d (Beckman Coulter). For viability analyses ...
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bioRxiv - Biochemistry 2021Quote: ... 1 h 10 min (45Ti, Beckman), resuspended in lysis buffer and stored in aliquots at –80°C until use ...
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bioRxiv - Bioengineering 2020Quote: ... 1X 1 μm SPRI beads (Beckman) in 1X PBS buffer with 0.5% Tween20 were injected into the thin-chip ...
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bioRxiv - Cancer Biology 2020Quote: ... GlyA PE (1:100, Beckman Coulter), CD71 BV650 (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: ... GlyA PE (1:100, Beckman Coulter), CD71 BV650 (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: ... CD4 PC5 (1:100, Beckman Coulter) and CD56 BV605 (1:100) ...
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bioRxiv - Cancer Biology 2020Quote: ... GlyA PE (1:100, Beckman Coulter), CD71 BV650 (1:100) ...
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bioRxiv - Microbiology 2022Quote: ... or 1:300 KC57-RD1 (Beckman Coulter 6604667 in BD Perm/Wash Buffer for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... CD56 (NKH-1-RD1, Beckman Coulter), ERK1/2 (T202/Y204 ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
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bioRxiv - Immunology 2024Quote: ... Unsupervised clustering of reduced clinical data was performed by FlowSOM (hierarchical consensus clustering; 25 clusters, 5 metaclusters, 10 iterations).81 All analyses were performed using Cytobank (Beckman Coulter Inc., Brea, CA).
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bioRxiv - Cell Biology 2023Quote: ... The filters were washed with 5 ml of cold wash buffer and then counted using a liquid scintillation counter (Beckman Coulter LS6500 scintillation counter).
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bioRxiv - Bioengineering 2024Quote: ... The DNase I reaction was inactivated with 5 mM EDTA and then subsequently cleaned up with 1X RNA SPRI beads (Beckman Coulter cat. no. A63987). 500 ng of RNA per sample was reverse transcribed using an iScript cDNA Synthesis kit (Bio-Rad cat ...
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bioRxiv - Molecular Biology 2020Quote: ... All amplified cDNA was purified using a 1:1 ratio of AMPure XP beads (Beckman Coulter, USA), and resuspended in 25 ml ...
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bioRxiv - Genomics 2021Quote: ... and short fragments were removed by mixing 1:1 by volume with AMPureXP magnetic beads (Beckman Coulter) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and 1 mM dithiothreitol) and spun for 1 h at 100,000 rpm using TLA-110 rotor (Beckman) at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... Samples were purified with a 1:1 ratio of AMPure XP SPRI beads (Beckman Coulter – cat#A63880) and resuspended in 51μl EB ...
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bioRxiv - Developmental Biology 2023Quote: ... Pre-amplified cDNA was purified with SPRI AMPure XP beads (Beckman, sample to beads ratio 1:1) and eluted in 15 μl Buffer EB (QIAGEN) ...
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bioRxiv - Plant Biology 2020Quote: ... The constructed libraries were size-selected using 1:1 volume of AMPure XP beads (Beckman Coulter, Brea CA). Size and quality of libraries were examined using Bioanalyzer 2100 (Agilent ...
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bioRxiv - Microbiology 2020Quote: Amplified cDNA was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 48μL of water ...
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bioRxiv - Microbiology 2020Quote: Amplified cDNA was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 48μL of water ...