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Citations for Beckman :
351 - 394 of 394 citations for Lipoteichoic Acid LTA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The amplified DNA was purified using a Qiagen MinElute PCR Purification Kit and primer dimers (<100 bp) were removed using AMPure beads (Beckman Coulter). ATAC-Seq was performed with two biological repeats to ensure the robustness of the data sets ...
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bioRxiv - Microbiology 2021Quote: ... The first and second PCR reaction products were purified using AMPure XP PCR product cleanup and size selection kit (Beckman Coulter), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The library preparation was completed by ligation of adapters from the SQK-LSK109 kit (Oxford Nanopore Technologies) and cleaned up with AMPure XP beads (Beckman Coulter) before preparation for sequencing ...
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bioRxiv - Microbiology 2020Quote: Library preparation was performed using Illumina NexteraXT™ DNA Sample Prep Kit per manufacturer’s protocol followed by AMPure XP (Beckman Coulter) purification ...
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bioRxiv - Cancer Biology 2020Quote: ... 200ng of total RNA was first treated to remove the rRNA and then purified using the Agencourt RNA Clean XP Kit (Beckman Coulter) prior to analysis with the Agilent RNA 6000 Pico Chip to confirm rRNA removal ...
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bioRxiv - Immunology 2022Quote: ... The presence or absence of fecal blood in fresh pellets was determined by macroscopic observation or by applying wet fecal samples to detection tabs from a Hemoccult blood testing kit (Beckman Coulter).
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bioRxiv - Cancer Biology 2022Quote: ... using MiSeq V2 300 cycle reagent kit after the amplified library was purified with AMPure XP beads (Beckman Coulter, CA, USA) (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
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bioRxiv - Microbiology 2022Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The PCR amplicons of the samples were then pooled after a purification and concentration equalization process with the AMPureXP Kit (Beckman Coulter). The libraries were processed in an Illumina MiSeq v2 (500 cycles and paired-end).
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bioRxiv - Cell Biology 2023Quote: ... RNA was processed using a NEBNext Ultra II Directional RNA library Prep Kit for Illumina paired with a Poly(A) mRNA magnetic isolation module and AMPure XP SPRIselect beads (Beckman Coulter). Libraries were sequenced using a NextSeq 500 High Output sequencer (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were purified using Qiagen MinElute PCR purification kit and size-selected (∼30-280 bp) using Ampure XP beads (Beckman Coulter). Reporter plasmid libraries were made by cloning amplified ATAC fragments into AgeI-HF- and SalI-HF-linearized pSTARR-seq plasmid using InFusion HD cloning kit (Takara ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products from thew first PCR were individually purified using 0.6X volumes of AMPure XP magnetic beads (AMPure XP beads kit / Beckman coulter, USA), followed by a second PCR reaction to attach the dual indices (S5 and N7 ...
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bioRxiv - Immunology 2023Quote: ... Final PCR reaction was then purified with a MinElute PCR Purification Kit followed by size-selection (150bp-1000bp using Ampure XP beads, Beckman Coulter). Sequencing was performed using a MGISEQ-2000 (BGI Tech Solutions) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10–20 ng/μl using a high-fidelity DNA polymerase and purified with the Agencourt AMPure XP Kit (Beckman Coulter Genomics). The cDNA samples were pooled based on the ratios according to the RNA concentrations of the input samples ...
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bioRxiv - Biochemistry 2019Quote: ... low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) using commercially-available kits (Beckman-Coulter, CITY, STATE, USA). Serum insulin concentration was determined using an electrochemiluminescence-based assay (Immulite 2000 ...
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bioRxiv - Microbiology 2019Quote: ... The cDNA pool was size fractionated in the size range of 200 to 600 bp using a differential cleanup with the Agencourt AMPure kit (Beckman Coulter Genomics). Aliquots of the cDNA pools were analyzed by capillary electrophoresis (Shimadzu MultiNA microchip) ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were purified with the kit from Agencourt AMPuer XPsystem (Cat. No. A63880, Beckman Coulter, South San Francisco, CA, USA), and the purified products were used with the second PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... The pulverized tissue was solubilized in pre-heated lysis buffer AL and RNA was extracted using the RNAdvance Tissue Kit (Beckman Coulter, Germany) in combination with a KingFisher Flex Purification System (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained both for extracellular and intracellular markers using the PerFix-nc kit following the manufacturer’s protocol (Beckman Coulter, Indianapolis, USA). The following antibodies were used during the staining protocol ...
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bioRxiv - Immunology 2022Quote: The stimulated PBMCs were washed twice with PBS pH7.2 buffer and then permeabilized using the PerFix-nc® reagent kit (Beckman Coulter, USA). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The V4 region of 16S rRNA gene was amplified using the universal primer set 515F and 806R.27 PCR products were cleaned up with an AMPure XP kit (Beckman Coulter, CA), followed by electrophoresis on 0.7% agarose gel and 1.15% Synergel (Diversified Biotech ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... we used an AV-FITC/PI apoptosis detection kit to detect programmed cell death using flow cytometry (Cytoflex, Beckman Coulter, CA, USA), as previously described (49 ...
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bioRxiv - Cell Biology 2023Quote: Myf5High and Myf5Low MuSCs were isolated from a pool of 4 male and 4 female mice and repeated in two independent experiments and total RNA was extracted using Agencourt RNAdvance tissue Kit (Beckman Coulter, #A32646) with a quality score of RQN 9.3 to 10 ...
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bioRxiv - Microbiology 2023Quote: ... rRNA was depleted using the NEBNext® rRNA Depletion kit (Bacteria) and purified from the enzymatic reactions using RNAClean XP magnetic beads (Beckman Coulter) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The linearized backbone was extracted from the gel using the Zymogen Gel DNA recovery Kit (#D4001) followed by additional clean up using 1x SPRI bead selection (Beckman Coulter, #B23318) and eluted in TE buffer ...
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bioRxiv - Microbiology 2024Quote: ... The resulting cDNAs were PCR-amplified to about 10-20ng/µl using a high-fidelity DNA polymerase and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). Pooled libraries were sequenced on an Illumina NextSeq 500 platform at the Core Unit SysMed at the University of Würzburg for bacterial in vitro samples and on an Illumina Novaseq at the genome analytics core facility of the Helmholtz Centre for Infection Research (HZI ...
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bioRxiv - Cancer Biology 2024Quote: ... Apoptotic cells were analyzed with the Ebioscience™ Annexin V Apoptosis Detection Kit APC according to the kit instructions (Cat. 88-8007-74, Thermo Fisher Scientific. Samples were read through the CytoFLEX S (Beckman Coulter) instrument using the APC laser to detect the Annexin-V+ cells ...
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bioRxiv - Molecular Biology 2020Quote: ... entire ChIPed DNA or 300ng of the input DNA were used to construct libraries using NEBNext® Ultra™ II DNA Library Prep kit with AMPure XP magnetic beads (Beckman Coulter). Library quality and quantity were estimated with Bioanalyzer and Qubit assays ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from fresh frozen tissue or cell lines using the Agencourt DNAdvance Beckman Coulter kit (#A48705, Beckman Coulter, Brea, CA, USA), fragmented and submitted to a series of enzymatic steps including End-repair and Adenylation ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were checked on agarose gel for successful amplification and purified with an Agencourt AMPure XP kit (Beckman Coulter Inc., Brea, CA, USA) using the supplier’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were then pooled for each of the 16S rRNA and fungal ITS1 regions after a purification/equalization process with the AMPureXP Kit (Beckman Coulter, Inc., Brea). Primer dimers ...
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bioRxiv - Cell Biology 2023Quote: ... were collected from microplates and washed twice in PBS and then resuspended in 50 μl of detergent (DNA-Prep Reagent Kit; Beckman Coulter, Brea, CA) containing 10 ng/ml propidium iodide (DNA-Prep Reagent Kit ...
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bioRxiv - Genomics 2024Quote: ... All DNA samples were cleaned three times using Agencourt AMPure beads prior to starting the 1D library kit (Beckman Coulter, Inc., CA, USA). Genomes were assembled with Canu v1.6 with a minimum read length of 5 kbp (45) ...
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bioRxiv - Immunology 2023Quote: ... GEMs were broken and complementary DNA (cDNA) was cleaned up with DynaBeads MyOne Silane Beads (10x Genomics, No. 2000048) and SPRIselect Reagent Kit (Beckman Coulter, No. B23318). Full-length ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We prepared sequencing libraries using the New England Biolabs Ultra II kit with the only modification being the use of Ampure XP beads for cleanup and size selection (Beckman Coulter, Brea, CA, USA). Following adapter ligation a single size selection was conducted at 0.65X ...
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bioRxiv - Evolutionary Biology 2021Quote: ... one half was purified using silica columns (MinElute PCR Purification Kit) and the other using 3x SPRI beads (Beckman Coulter™ Agencourt AMPure XP). Indexing PCR ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from all TP53-KO minor cells treated in F10 matrigel assays using the Agencourt DNAdvance Genomic DNA Isolation Kit (Beckman Coulter, Cat. No. A48705), and the TP53 locus was amplified using the following primers ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were collected and RNA was isolated using the Biomek FxP and the RNAdvance Tissue Isolation kit (Beckman Coulter, USA, p/n A32646). The resulting RNA was quantified using the Nandrop 8000 (Nanodrop ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Microbiology 2019Quote: ... 4th and 8th week using assay kits (BioSino Bio-Technology & Science Inc., Beijing, China) and the AU480 Chemistry analyzer (Beckman Coulter Inc., California, USA).
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bioRxiv - Microbiology 2021Quote: ... Any amplicon contaminated with traces of non-specific fragments was further purified using the Agencourt AMPure™ PCR purification kit (Beckman Coulter, Beverly, MA, USA). The concentration of each PCR amplicon was determined using the Quant-iT™ PicoGreen kit (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... The DNA was end-prepped with the NEBNext FFPE Repair and Ultra II End Prep Kit and purified using Agencourt AMPure XP (Beckman Coulter Inc., Brea, CA, USA). Then the adapter ligation was performed using NEBNext Quick T4 DNA Ligase ...