Labshake search
Citations for Beckman :
351 - 400 of 454 citations for Fumarase from porcine heart since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... Eluates from gel extraction were purified using a 1.8x bead cleanup with AMPure XP beads (Beckman Coulter cat. no. A63881). Adapter-ligated DNA libraries were cloned into purified pSTARR-seq_fly using a 2:1 molar ratio of insert (size determined via TapeStation ...
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bioRxiv - Genetics 2024Quote: Pieces of caudal fin sampled from those 315 fish were sent to Gentyane genotyping platform (INRAE, Clermont-Ferrand, France) for genotyping after DNA extraction using the DNA advance kit from Beckman Coulter following manufacturer instructions.
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bioRxiv - Cancer Biology 2021Quote: ... genomic DNA from the LuCaP PDXs was sheared using a Covaris Sonicator E220 and size selection performed with AMPure XP beads (Beckman Coulter) to retain 150-250 bp DNA fragments ...
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bioRxiv - Cell Biology 2020Quote: Interstitial macrophages (CD45+CD64+Siglec-F−CD11bhigh) or alveolar macrophages (CD45+CD64+Siglec-F+CD11blow) were isolated from C57BL/6 by MOFLO system (Beckman Coulter). Isolated macrophages were cultured for 24 hrs in RPMI-1640 medium containing 10% FBS and 50μM 2-mercaptoethanol with or without GM-CSF (10 ng/ml).
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bioRxiv - Biophysics 2022Quote: ... From each sample 100 µl lysate were transferred to a special tube for ultra-centrifugation (Beckman Coulter, 1.5 ml tube, 257448). The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80 ...
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bioRxiv - Genomics 2020Quote: The ∼400 bp amplicon product produced using both Pool-1 and Pool-2 from the same clinical sample was combined and purified using a 1X concentration of Agencourt AMPure XP beads (Beckman Coulter); the final product was eluted in 30 µl of elution buffer (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... the resin was spun down by centrifugation and nucleic acids were purified from supernatant with AMPure XP magnetic beads (Beckman Coulter). As a control ...
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bioRxiv - Genomics 2020Quote: ... Equal quantities of the four PCR products from each mosquito pool were mixed and purified using Agencourt AMPure XP (Beckman Coulter).
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bioRxiv - Cancer Biology 2020Quote: Samples collected from bone marrow and peripheral blood were deposited onto lysine coated slides using StatSpin CytoFuge (Beckman Coulter, Indianapolis, IN). Briefly ...
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bioRxiv - Plant Biology 2021Quote: ... and 3′ RNA-seq libraries were prepared robotically from 500 ng total RNA in 96-well plates on an NXp liquid handler (Beckman Coulter) using QuantSeq FWD kits (Lexogen ...
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bioRxiv - Microbiology 2021Quote: ... PCR products from mouse samples were gel purified whereas PCR products from ferret samples were purified with paramagnetic beads using the AMPure XP for PCR Purification kit (Beckman Coulter). Purified PCR products were used in a second PCR reaction for incorporating sample-specific 5’-end indexes and additional Illumina sequencing adapters (Supplementary Table 2) ...
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bioRxiv - Cell Biology 2020Quote: ... with densities ranging from 1.10 to 1.15 were pooled and washed in PBS (Beckman Ti50, 3 h at 42 000 rpm).
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bioRxiv - Cancer Biology 2022Quote: ... vehicle control or compounds from the small molecule library (Supplementary Table 4) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were then incubated in an IncuCyte Zoom (Sartorius ...
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bioRxiv - Molecular Biology 2022Quote: ... the products from the same library were pooled and fragments ranging in size from 300-500 bp were selected with AMPure XP beads (Beckman Coulter). The size-selected libraries were sequenced to produce paired-end Hi-C reads on the Illumina HiSeq 2500 platform with the V4 of 125 cycles.
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bioRxiv - Microbiology 2020Quote: ... and mean corpuscular hemoglobin concentrations were analyzed from blood collected in tubes containing EDTA using a laser-based hematologic analyzer (Beckman Coulter). Serum samples were tested for concentrations of alanine aminotransferase (ALT) ...
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bioRxiv - Neuroscience 2021Quote: ... Labeling was done with anti-HLA-DR-PB and anti-CD16-FITC (Duraclone pre-coated tubes or separate antibodies, all from Beckman-Coulter) and anti-CD14-PEvio770 (Miltenyi ...
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bioRxiv - Neuroscience 2021Quote: ... 50 μL of PCR product from the pre-amplification step were purified using 50 μL of Ampure XP beads (Beckman Coulter), resuspended ...
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bioRxiv - Immunology 2020Quote: ... erythrocytes in 2 μl blood (collected from the tail tip) were counted using a Z2 Coulter Particle count and size analyser (Beckman Coulter). We determined the healthy range in uninfected C57Bl/6J mice housed in our facility to be 8.8 - 10.5*109 erythrocytes*ml−1 ...
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bioRxiv - Genomics 2020Quote: ... The library preparation was completed by ligation of adapters from the SQK-LSK109 kit (Oxford Nanopore Technologies) and cleaned up with AMPure XP beads (Beckman Coulter) before preparation for sequencing ...
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bioRxiv - Immunology 2021Quote: ... Amplified cDNA was purified and separated from amplified HTOs using a 0.6x size selection via SPRIselect magnetic bead (Beckman Coulter #22667) and a 1:10 dilution of the resulting cDNA was run on a Fragment Analyzer (Agilent Technologies #5067-4626 ...
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bioRxiv - Genomics 2021Quote: ... antibody-oligo derived cDNA fragments were separated from mRNA derived cDNA through SPRI-based size selection by incubating cDNA amplification product in 0.6x SPRIselect (Beckman Coulter B23319) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... CX3CR1-GFP and GFAP-CFP cells from adult male and female mice were submitted to FACS immediately after dissociation using a MoFlo Astrios EQ (Beckman Coulter) based on their fluorophore (GFP and CFP) ...
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bioRxiv - Systems Biology 2023Quote: ... all reactions from the same sample were pooled and purified with Agencourt AMPure XP SPRI beads according to the manufacturer protocol (Beckman Coulter). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal optical density units from cleared cell lysates were loaded onto the gradient and centrifuged in a SW40 rotor (Beckman Coulter) for 5 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... The sediment from the smaller core was dried for grain size measurement using a LS13320 Particle Size Analyzer (Beckman-Coulter, USA).
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bioRxiv - Microbiology 2023Quote: ... Fluorescent LEVs and SEVs from the corresponding pellets were analysed by flow cytometry (FC) on a Gallios Flow Cytometer (Beckman Coulter) using an optimised procedure ...
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bioRxiv - Immunology 2024Quote: Individual CD24hi or CD24low Vγ1.1+ γδ T cells were isolated from Id3+/+ and Id3-/- mice by flow cytometry and deposited in wells of glass slides (Beckman-Coulter). Vγ1.1 and Vδ6.3 subunits were then amplified by RT-PCR using nested primers in single cells ...
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bioRxiv - Microbiology 2023Quote: ... RBD expression and ACE2 signal were recorded by using an automated acquisition from 96 well plates by CytoFLEX Flow Cytometer (Beckman Coulter), background binding signals were subtracted ...
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bioRxiv - Cell Biology 2023Quote: The genotypes of the chimeric mice were confirmed using genomic DNA extracted from GFP-negative fibroblasts obtained using FACS (MoFlo XDP, Beckman Coulter). MEFs were isolated from the entire fetus and head tissues on embryonic days (E ...
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bioRxiv - Biophysics 2023Quote: ... Plasmids were transferred from 96-well plates into 384-well plates using a Biomek FX Automated Workstation (Beckman Coulter, model A31843). Each plasmid was pipetted into 4 consecutive wells within the 384-well plate ...
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bioRxiv - Biophysics 2023Quote: ... and subsequently removed from the substrate and rinsed with PBS before measuring using a flow cytometer (CytoFLEX S flow cytometer, Beckman Coulter).
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bioRxiv - Immunology 2022Quote: ... The presence or absence of fecal blood in fresh pellets was determined by macroscopic observation or by applying wet fecal samples to detection tabs from a Hemoccult blood testing kit (Beckman Coulter).
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bioRxiv - Cell Biology 2022Quote: ... A 12 ml 2 % step Optiprep gradient in LB ranging from 32 % to 10 % was prepared beforehand in Ultra-Clear tubes (Beckman Coulter). Samples were spun for 16 hours at 100000 g at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Amplified cDNA was purified and separated from amplified HTOs using a 0.6x size selection via SPRIselect magnetic bead (Beckman Coulter #22667) and a 1:10 dilution of the resulting cDNA was run on a Fragment Analyzer (Agilent Technologies #5067-4626 ...
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bioRxiv - Immunology 2022Quote: CD27+ IgM- B cells cross-binding to SARS-CoV-1 and SARS-CoV-2 RBD were sorted from SARS-CoV-2 vaccinated SARS convalescents with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... MULTI-seq barcode fragments were separated from endogenous cDNA fragments during the first round of size selection using SPRIselect beads (Beckman coulter). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... The muscle ECs (CD31+CD45-DAPI-) from single cell suspension of total muscle cells were obtained by FACS sorting using MoFlo Astrios (Beckman Coulter) or also isolated using mouse CD31 microbeads (130-097-418 ...
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bioRxiv - Genomics 2022Quote: ... The sample collected from the BluePippin instrument was cleaned-up and concentrated using an equal volume of AMPure XP Beads (Beckman Coulter), with the DNA eluted in in 60 μL DNAse and RNAse free water ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Circulating serum estradiol (E2) from all behaviorally characterized rats was quantified using radioimmunoassay (RIA) according to the manufacturer’s protocol (Beckman Coulter, DSL4800). The RIA was conducted in a single cohort and samples were analyzed in duplicate ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted from fin clips and embryos using the Agencourt DNAdvance magnetic bead-based purification system (Beckman Coulter, Brea, CA) in a 96-well plate format ...
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bioRxiv - Microbiology 2024Quote: ... We used the same gating strategy to identify and collect active cells from each sample with FACS on a MoFlo Astrios Cell Sorter (Beckman Cloutier). To collect viable cells with FACS ...
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bioRxiv - Genomics 2024Quote: ... The PCR products from each reaction were combined then purified using an equal volume of Ampure XP SPRI Reagent (Cat# A63881, Beckman Coulter) as per the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... was obtained from total peripheral EDTA blood samples by extraction of white blood cells with a Biomek FX or i7system (Beckman Coulter) using the NucleoMag® Blood 3 ml Kit (Machery-Nagel ...
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bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Vesicles were collected from cell-free supernatant culture by ultracentrifugation at 100000 × g (centrifuge Optima XPN-100 Beckman Coulter and rotor SW28) at 4 °C for 1.5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuoles were separated from free dye by centrifugation (100,000 x g, 25 min, 4°C, SW-60 Ti rotor, Beckman L8-80M Ultracentrifuge) and recovered from the 0%-4% interface ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus from the cell supernatant was collected 24h later by ultracentrifugation with a Sw28 rotor (Beckman Coulter; 22,000 rpm, 2h, 4°C). Finally ...
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bioRxiv - Microbiology 2021Quote: ... PCR products from two wells per sample were pooled and purified with AMPure XP beads according to the manufacturer’s protocol (Beckman Coulter, #MSPP-A63880). Samples were sequenced on a NextSeq550 sequencer (Illumina) ...
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bioRxiv - Genomics 2021Quote: Sequencing library amplified from the second round of PCR were size-selected and purified with a magnetic bead-based SPRIselect reagent (Beckman Coulter, B23318), and subjected to HiSeq4000 Illumina NGS platform using a single read (SR75 ...
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bioRxiv - Biochemistry 2022Quote: ... Average fluorescence intensity of DCF from 3×105 cells was recorded and data acquisition and analysis were carried out using Summit 5.2 software (Beckman-Coulter, Inc. USA). Morphology of algal cells was examined and photographed with a differential interference contrast microscopy (DIC ...