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Citations for Beckman :
351 - 400 of 467 citations for 7 9 Diazaspiro 4.5 decane 6 8 10 trione 7 ethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... for 30 min at 37°C and for 10 min at 80°C followed by a 1.2X AMPure XP beads (Beckman Coulter) cleanup ...
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bioRxiv - Microbiology 2022Quote: ... was added before ultracentrifugation at 20,000 × g for 2 hours at 10°C in an Optima L-90K Ultracentrifuge with the SW32Ti rotor (Beckman Coulter). Supernatants were carefully discarded by aspiration and the 6 phage pellets were resuspended with 0.1 ml of TM buffer (50 mM Tris-HCl ...
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bioRxiv - Genomics 2019Quote: ... Resulting cDNA was purified by a 10 min incubation with 144 µl (1.8X) Agencourt AMPure XP beads (A63880, Beckman Coulter), 2x 200 µl washes with 80% ethanol for 30 sec each ...
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bioRxiv - Genomics 2019Quote: ... Resulting DNA was purified by a 10 min incubation with 70 µl (0.7X) Agencourt AMPure XP beads (A63880, Beckman Coulter), 2x 200 µl washes with 80% ethanol for 30 sec each ...
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bioRxiv - Microbiology 2020Quote: VLPs were collected after removal of cells from the enrichment culture (7,000 rpm, 20 min, Sorvall 1500 rotor) and concentrated by ultracentrifugation (40,000 rpm, 2h, 10°C, Beckman SW41 rotor). The particles were resuspended in buffer A ...
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bioRxiv - Cell Biology 2019Quote: ... and spun down at 4° C for 10 minutes at 12096×g using an Avanti J-25 centrifuge (Beckman Coulter). The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was then slowly mixed with an equal volume of 4 M ammonium sulphate (2 M final concentration) at 4°C for 1 hour and spun at 10 K rpm using a JA-17 rotor (Beckman). Pellets containing RAD51 were suspended in 25 ml of resuspension buffer (0.5 M KCl ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant from 2,000 × g was transferred to 30mL conical tube and then centrifuged at 10,000 × g for 10 min at 4°C (# Avanti J-E JA25-50 Beckman Coulter). The supernatant from this spin was filtered through a 0.22-μm mesh filter (# SLGP033RS EMD Millipore ...
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bioRxiv - Genomics 2019Quote: ... A pool of seven gDNA extractions from fragment section 3_10-10 was re-purified with AMPure XP Beads (Agencourt, Beckman Coulter) and eluted in 50ul of water ...
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bioRxiv - Plant Biology 2020Quote: ... the cells were harvested by centrifuging for 30 min at 4°C and 6,000 rpm (JA-10, Beckman Coulter®). The cells were resuspended in 1x PBS (pH 7.3 ...
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bioRxiv - Bioengineering 2020Quote: ... microbubbles rested for 10 min before the suspension was mixed and diluted to 0.2% v/v in Isoton (Beckman-Coulter). For each vial ...
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bioRxiv - Biochemistry 2019Quote: ... The homogenate was centrifuged for 10 min at 10,000×g at 4°C in a Ti-70 rotor and Optima XPN-90 ultracentrifuge (Beckman Coulter) and the supernatant was removed ...
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bioRxiv - Microbiology 2020Quote: ... diluted to 20 mL with PBS and pelleted through a cushion of 10% sucrose in PBS to further clean and concentrate the sample (SW32 Beckman centrifuge tube at 25,000 rpm at 4°C for 2 h) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The pellet was resuspended in 60% Percoll in STE and loaded on the bottom of six 10-35% Percoll gradient in STE in polycarbonate tubes for SW28 rotor (Beckman). Gradients were centrifuged for 1 hour at 24000 rpm ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Genomics 2022Quote: ... Lysates were fractionated on a 10% to 60% sucrose gradient using the SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter) at 36,000 rpm for 3 h and 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... step gradients were made by superposing sucrose solutions of different concentration (50%, 40%, 30%, 20%, 10%) in an ultra-clear polycarbonate tube (Beckman), and a linear gradient was allowed to form overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was dounced for 10 strokes on ice and cleared via centrifugation at 80K rpm for 10 minutes at 4°C using a TLA 110 rotor (Beckman) in a tabletop Beckm an ultracentrifuge ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was cleared by two rounds of centrifugation at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter) followed by centrifugation at 31,900 ×g (10 min ...
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bioRxiv - Genomics 2023Quote: ... Precipitated ribosomes were pelleted at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). To remove residual supernatant the pellet was re-centrifuged for 5 min at 20,000 ×g ...
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bioRxiv - Genomics 2023Quote: ... The suspension was incubated on ice for 5 min and centrifuged at 20,000 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). The pellet was re-centrifuged for 5 min and both supernatants were combined into a new tube ...
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bioRxiv - Cancer Biology 2023Quote: ... The ribosomes with equal A260 in each group were loaded onto a sucrose gradient (10–50%) buffer prepared using a gradient station and centrifuged in an SW41Ti rotor (Beckman) at 4°C for 4 hours at 28,000 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... The density adjusted FBS was subjected to ultracentrifugation for 25 h at 310,500 x g at 10°C in a 70Ti rotor (Beckman Coulter) before dialysis ...
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bioRxiv - Cancer Biology 2023Quote: ... 7,500 GFP+ and 7,500 RFP+ single live cells were sorted into each well of a 24 well culture plate containing low pH DMEM with 10% FBS using a MoFlo Astrios cell sorter with Summit v6.3 software (Beckman Coulter). Sorting was performed at 25psi using a 100um nozzle ...
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bioRxiv - Immunology 2023Quote: ... at 950 rpm for 10 min at 20°C and centrifuged at 15,000 rpm (Beckman Coulter Microfuge® 22R refrigerated microcentrifuge, Beckman Coulter Australia Pty Ltd ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The whole cell homogenate was layered on top of 2 ml shelf of 0.8 M sucrose in HB and centrifuged at 2000 rpm for 10 min at 4°C in a JS5.2 rotor (Beckman Coulter) to yield a low speed nuclear pellet and a postnuclear supernatant ...
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bioRxiv - Cancer Biology 2023Quote: Cell death was evaluated 48 hrs after ionizing radiation (10 Gy) by using a CytoFLEX S (Beckman Coulter, Milan, Italy) instrument ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Illumina 16S Metagenomic Sequencing Library Preparation Protocol with the following changes: 10 μL of Amplicon product was purified using a 1:0.8 ratio of XP beads (Beckman Coulter), with beads resuspended in a final volume of 10 μL of nuclease-free water ...
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bioRxiv - Molecular Biology 2024Quote: ... the mixture was spun at 13,894 rpm (16,000 rcf) at 4 ºC for 10 min using the SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). The beads that accumulated at the bottom of the tube were resuspended in 200 µL of EM buffer A ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were pelleted at 280’000 x g for 10 min at 25 °C in an Optima XP centrifuge with a TLA-100 rotor (Beckman-Coulter). Supernatant and pellet fractions were separated on a 10 % SDS-PAGE gel and proteins were visualized by Coomassie Blue staining ...
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bioRxiv - Molecular Biology 2021Quote: ... About 1,5 mg of protein was loaded on a 10–50% sucrose gradient and centrifuged for 2h45min at 39 000 rpm in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41 rotor (Beckman) ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast cells were stained with 5ug\ml propidium iodide for 10 minutes and samples were examined in CytoFlex flow cytometer (Beckman Coulter) using PE channel ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged through a 10–35% sucrose gradient for 19 h at 16,000 rpm in a SW28 rotor (Beckman Coulter, Inc.). Fractions containing the 70S peaks were pooled and kept at −80℃ for further use.
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bioRxiv - Bioengineering 2022Quote: ... The 60-70 mL were then divided equally into two 50-mL centrifuge tubes and spun down outside the anaerobic chamber at 4 °C and 3500 rpm for 10 min using an Allegra 64R ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Bioengineering 2022Quote: ... Each 250 mL culture was divided equally into six 50-mL centrifuge tubes and spun down at 4 °C and 3500 rpm for 10 min using an Allegra 64R centrifuge (Beckman Coulter). The supernatant was discarded and 6 mL of ice-cold 10% glycerol was used to wash and combine the six cell pellets into one suspension ...
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bioRxiv - Genetics 2020Quote: ... The homogenates were incubated on ice for 15–20 min and then centrifuged at 3000xg for 10 min at 4°C in a GPR Data Settop centrifuge (Beckman, Inc.). The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042 ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was overlaid with decreasing concentrations of Nycodenz (30%, 20%, 10% and 0%) and centrifuged in a SW55 rotor (Beckman Coulter) 2h 30 min at 55000 rpm and 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Counting was performed twice for each sample (10 min per sample) with a ß-scintillation counter Beckman LS6000TA (Beckman Coulter, USA). Roots of two plants identically grown and processed but not exposed to the incubation solution were used to determine background counts ...
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bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10-20 ng/μl using a high-fidelity DNA polymerase followed by purification using the Agencourt AMPure XP kit (Beckman Coulter). The cDNA samples were pooled with ratios according to the RNA concentrations of the input samples and a size range of 200-550 bp was eluted from a preparative agarose gel ...
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bioRxiv - Cell Biology 2022Quote: ... which was then slowly poured on top of 10 ml cushion solution (2.05M sucrose with protease inhibitors, DTT and PMSF) in the ultracentrifugation tube (Beckman polyallomer #326823). After a 45-min spin at 24,600 rpm in pre-chilled SW32Ti rotor at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Putative LSC-enriched populations were isolated from freshly expanded MLL-AF9 mouse leukemia cells by sorting c-Kithigh (top 10%) cells on a MoFlo XDP cell sorter (Beckman Coulter). Sorted cells were plated in triplicate in semi-solid methylcellulose medium (Methocult M3434 ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... cryopreserved PBMC were recovered overnight in RPMI1640 supplemented with 10% FBS and washed in cold phosphate buffer (PBS) before suspended in cold PBS and sorted on Moflo Astrios (Beckman Coulter). The excitation wavelength for the generation of side-scatter (488/6-nm filter ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate was incubated under rotation for 30 min at 4 °C and cleared for 10 min in an MLA-80 rotor (Beckman Coulter) at 11,500 xg and 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... The assembled complexes were incubated for 5 min at 30 °C and for 10 min on ice before they were loaded on a 30% (w/v) sucrose cushion in TLA-100 tubes (Beckman Coulter). The sucrose cushions were centrifuged for 2 h at 390,880 xg and 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The equivalent of 200 µl of cell extract with an OD260 of 12 was loaded onto a 10–50% sucrose gradient and centrifuged in a SW41 rotor (Beckman Coulter) at 39,000 rpm for 2.45 h at 4°C ...
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bioRxiv - Synthetic Biology 2021Quote: ... The complete NanoTrap was purified using rate-zonal centrifugation33 through a 1545% glycerol gradient in 1×TE + 10 mM MgCl2 in an SW 55 rotor (Beckman Coulter). Fractions were collected after a 1 hr centrifugation at 50 k rpm ...