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Citations for Beckman :
351 - 400 of 2152 citations for 4 4 Fluoro 2 hydroxyphenyl methyl 2 6 bis 1 methylethyl 5 propyl 3 pyridinemethanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... at 4 °C in an Avanti J26 super centrifuge (Beckman Coulter). The supernatant containing soluble His-SUMO-GLT25D1/COLGALT1 protein was filtered through a MiniSart GF 0.8 μm filter (Sartorius) ...
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bioRxiv - Genetics 2024Quote: 2 mL AMPure XP beads (Beckman Coulter, A63882) were mixed thoroughly by vortexing ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... actins were cleared by highspeed centrifugation at 316,613 x g for 1 hour at 4°C in a TLA100 rotor (Beckman Coulter). Only the top 80% of the supernatant was collected to perform TIRF microscopy ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minutes 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). One aliquot of the eluted sample was resolved on SDS-PAGE ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The post nuclear supernatant was collected and separated on a stepwise sucrose density gradient and centrifuged at 100,000 g for 1 hour at 4°C in a swing-out rotor (Beckman coulter). The sucrose density gradients comprised 0.25 ...
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bioRxiv - Biochemistry 2022Quote: ... and the debris and membranes were removed by ultracentrifugation at 205,000 g for 1 hr at 4 °C (rotor 45 Ti, Beckman Coulter). The supernatants were applied to IMAC with Ni2+-NTA-agarose resin (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... The sucrose gradient was centrifuged at 24,400 rcf for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter), and nuclei at the bottom of the gradient were resuspended in PBS ...
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bioRxiv - Plant Biology 2021Quote: ... The thylakoid membranes with different densities were analytically separated at 125,000 × g and 4°C for 1 h (SW 41 Ti, Beckman Coulter).
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bioRxiv - Microbiology 2020Quote: ... OBs were loaded on to a discontinuous 35–60% (w/w) sucrose gradient and centrifuged at 20000 rpm and 4°C for 1 hour in a SW41 rotor (Beckman). The band containing the OBs was removed ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mM Tris-HCl) and centrifugated at 24,000 rpm for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS ...
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bioRxiv - Immunology 2020Quote: ... Then the concentrated supernatant was ultracentrifuged at 110,000 g for 1 hour at 4°C (Beckman Coulter L100k, company name) to sediment the vesicular fraction into a pellet ...
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bioRxiv - Microbiology 2022Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minute 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). For SDS-PAGE analyses ...
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bioRxiv - Molecular Biology 2023Quote: ... and ultracentrifuged in a sucrose cushion at 100,000 rpm at 4°C for 1 h with a TLA110 rotor (Beckman Coulter). The ribosome pellet was dissolved in TRIzol reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... Fractions 2–9 were diluted 1:10 in PBS and centrifuged at 20,000 ×g (bEVs) or 100,000 ×g (sEVs) at 4°C for 1 h with an MLS-55 rotor (Beckman Coulter). The bEV and sEV pellets were suspended in double 0.22-µm-filtered PBS and were either used directly for experiments or stored in aliquots at −80°C until subsequent BLI ...
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bioRxiv - Neuroscience 2023Quote: Monomeric wild-type α-syn was ultracentrifuged at 90,000 RCF for 1 hour at 4 °C to remove any amorphous aggregates (Beckman Coulter Optima Max-XP with MLA-130 rotor) ...
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Dopaminoceptive D1 and D2 neurons in ventral hippocampus arbitrate approach and avoidance in anxietybioRxiv - Neuroscience 2023Quote: ... 10 mM Tris-HCl) and centrifugated at 107,000 g for 1 hour at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS supplemented with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The final pellet containing the purified EV fraction was resuspended in 50 µl of 20 mM Tris-HCl pH 6.0 ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... loaded onto a discontinuous 0.75/1.0/2.33 M sucrose gradient and spin at 242,000 g for 1 h at 4 °C in a SW32Ti rotor (Beckman & Coutler). Growth cone fractions were collected at the interface load/0.75 M sucrose and non-growth cone fractions collected between 0.75/1.0 M sucrose and then analyzed by Western Blot.
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifugation at 100,000 rpm at 4°C for 1 h using TLA110 rotor (Beckman Coulter). RNAs in the ribosome pellet were isolated with TRIzol-LS (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The pellets from the tubes were pooled together in 500 µl of 20 mM Tris-HCl pH 6.0 buffer and the volume was brought up to 1,500 µl using 20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation for 1 h at 45,000 rpm at 4°C (Optima XPN-80 Ultracentrifuge with 70 Ti, Beckman Coulter). HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was ultracentrifuged at 80,000 × g for 1 h at 4°C using a type 50 Ti rotor ultracentrifuge (Beckman Coulter). The supernatant ...
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bioRxiv - Neuroscience 2024Quote: ... Digested samples were ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman Coulter) to concentrate the insoluble ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Plant Biology 2022Quote: ... in a 12.5 mL ultracentrifuge tube and centrifuged for 1 h at 4°C at 200,000 × g in a swinging bucket rotor (Sorval SW-41 Ti; Beckman Coulter), with moderate acceleration and deceleration ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris and unbroken cells were removed by centrifugation at 2,000 xg for 10 minutes and membranes were collected by ultracentrifugation at 186,000 xg for 1 hour at 4°C with a 45 Ti rotor (Beckman Coulter). The collected membrane was homogenized using a Dounce homogenizer in cold TBS buffer containing 1 mM PMSF ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was then transferred to polycarbonate tubes and centrifuged at 100,000 × g and 4°C for 1 h in a XL-90 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... This spin was repeated and the resulting supernatant was then centrifuged for 1 h at 75,000 g at 4°C (TLA-100.3 rotor or Type60 Ti rotor; Beckman Coulter, USA). The supernatant was aspirated and the membrane pellet gently resuspended in microsome buffer (10 mM HEPES/KOH pH 7.5. ...
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bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged for 1 h at 100,000 g and 4°C in a TLS 55 rotor (Beckman-Coulter) with the appropriate adapters (#358614) ...
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bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifuged at 100,000 rpm (543,000 × g) for 1 h at 4°C with a TLA110 rotor and an Optima MAX-TL ultracentrifuge (Beckman Coulter). Subsequently ...
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bioRxiv - Biophysics 2024Quote: ... the clarified lysate underwent a further centrifugation step at 205,000xg for 1 hour at 4 °C (60Ti rotor, Beckman Coulter). The membrane pellet was then resuspended in the resuspension buffer ...
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bioRxiv - Immunology 2022Quote: CD27+ IgM- B cells cross-binding to SARS-CoV-1 and SARS-CoV-2 RBD were sorted from SARS-CoV-2 vaccinated SARS convalescents with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Neuroscience 2021Quote: ... The gradient was centrifuged at 50,000xg for 21min at 4°C (Beckman Coulter Optima MAX XP ultracentrifuge with a TL-55 rotor) ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C for 30 min using a JLA 9.1000 centrifuge rotor (Beckman). E ...
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bioRxiv - Biochemistry 2020Quote: ... At OD600 1.2 the cells were pelleted at 4 °C (TLA10.500 (Beckman), 15 min at 5,000-8,000 rcf) ...
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bioRxiv - Immunology 2021Quote: ... Samples in Figure 4 were collected with a CytoFlex cytometer (Beckman Coulter). Collected cells were rinsed twice with PBS and stained with Zombie-UV viability dye (BioLegend) ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C for 30 minutes in an OptimaTM MAX Ultracentrifuge (Beckman, Germany). Supernatant was collected and stored at -80°C ...
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bioRxiv - Immunology 2023Quote: ... After centrifugation at 100,000xg for 16 hat 4°C (Beckman Ti50.2 rotor), the density of each fraction was measured ...
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bioRxiv - Cell Biology 2023Quote: ... for 20 hours at 4 °C in an ultracentrifuge (LE-80K, Beckman). The centrifuged medium was diluted with serum-free Opti-MEM supplemented with penicillin/streptomycin to a final concentration of 10% BGS and filtered using the Stericup-VP Sterile Vacuum Filtration System with a 0.1 µM PES filter (Merck) ...
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bioRxiv - Cell Biology 2024Quote: Cell volumes were measured using a Coulter counter (Multisizer 4, Beckman Coulter), which is based on the principle that a cell traversing an orifice filled with electrolyte will produce an impedance change proportional to the volume of the cell due to displacement of electrolyte ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C for 30 min using a JLA-9.1000 centrifuge rotor (Beckman). The pellet was resuspended in 500 mL hypotonic lysis buffer containing 10 mM Tris-HCl pH 7.8 ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C for 15 min using a JLA-9.1000 centrifuge rotor (Beckman). The pellet was resuspended in 70 mL buffer containing 50 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Final ultracentrifugation step at 100,000g/1hour/4°C (Beckman AirFuge CLS Ultracentrifuge) resulted in pellets which were resuspended in the same buffer (66) ...