Labshake search
Citations for Beckman :
301 - 350 of 1085 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
-
bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
-
bioRxiv - Bioengineering 2022Quote: ... Supernatant (33 mL) was placed on a 3 mL 25% (w/v) sucrose pad in SW28 tube (Beckman, Brea, CA, USA) and centrifuged at 24,000 rpm ...
-
bioRxiv - Cancer Biology 2023Quote: Counting of CB CD34+ cells after 3 days of ex vivo co-culture was accurately obtained with flow-count fluorosphere beads (Beckman Coulter), in combination with the following antibodies ...
-
bioRxiv - Microbiology 2024Quote: ... and resolved by ultracentrifugation at 36,000 rpm for 3 h at 4°C with the fastest braking in Optima XPN-80 Ultracentrifuge equipped with SW-41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 mL/fraction ...
-
bioRxiv - Immunology 2024Quote: ... Fluorescence signals and TruCount beads were acquired on a 3-laser CytoFLEX using Cytexpert software 2.3 (Beckman Coulter, Brea, CA, USA). Instrument quality control and standardization were performed daily using CytoFLEX Daily QC Fluorospheres (Beckman Coulter #B53230).
-
bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with chilled phosphate-buffered saline (PBS) and analyzed by flow cytometry (CytoFLex, Beckman Coulter Inc., Brea, CA, USA)[39 ...
-
bioRxiv - Cell Biology 2021Quote: ... The seeds were sedimented by centrifugation at 90,000 rpm for 5 min at 25 °C (Beckman Coulter). The microtubule pellet was resuspended in warm BRB80 buffer and microtubule seeds were stored in the dark at room temperature.
-
bioRxiv - Biochemistry 2022Quote: ... RNA was purified by denaturing gel electrophoresis (5% polyacrylamide) and by AMPure XP magnetic beads (Beckman Coulter). The purified RNA was buffer exchanged three times into RNase-free water using 30 kDa cutoff Amicon centrifugal filters (Millipore) ...
-
bioRxiv - Immunology 2024Quote: ... mApple-positive cells were sorted 5 days after infection on a Moflo XDP flow cytometer (Beckman Coulter) to obtain mApple-Mem-expressing Nalm6 cell lines.
-
bioRxiv - Cell Biology 2024Quote: ... The sample was transferred to a 250 µL ultracentrifuge tube (5 x 20 mm Beckman-Coulter, #342630) and a 50 µL of 10% sucrose cushion was placed below the sample at the bottom of the tube ...
-
bioRxiv - Microbiology 2024Quote: ... 5 mM MgSO4 and 2.1 M sucrose) and centrifuged at 23000 rpm (Beckman Ultracentrifuge, TLA-100.3 Rotor) for 5 min to remove small mycelial pieces and remaining intact cells ...
-
bioRxiv - Biochemistry 2024Quote: ... samples were ultracentrifuged at 100,000 g for 5 minutes at 25°C (Beckman TL100, rotor TL-A100). Supernatants were recovered and the hexapeptide concentrations of were determined by UV absorption as previously described ...
-
bioRxiv - Molecular Biology 2020Quote: ... MgCl2 5mM and Tris-Cl 50mM pH 7.4) was added into 3-mL polycarbonate tube for an ultracentrifuge TL100.3 rotor (Beckman Coulter, Brea, CA, USA). The KCl-adjusted ribosome-containing solution was carefully added above the sucrose cushion and tubes were balanced by weight using buffer B (sucrose 250mM ...
-
bioRxiv - Molecular Biology 2021Quote: ... Produced amplicons of 3-144 µm eukaryotes and Imitervirales were purified with the magnetic beads (Agencourt AMPure XP beads, Beckman Coulter, Inc.). Finial DNA production was dissolved in 27.5 µL ultrapure water ...
-
bioRxiv - Plant Biology 2021Quote: ... sucrose was transferred to the bottom of the tube using a Pasteur pipette and centrifuged at 27,000 rpm for 3 hours at 4° C using an SW28 Ti rotor (Beckman Coulter, Brea, CA). Each pellet containing the virus particles was resuspended in 1 mL resuspension buffer overnight with gentle shaking at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... supplemented with 100 μg/ml cycloheximide) and centrifuged for 3 hours at 35,000 rpm in an SW41 rotor in a Beckman L7 ultracentrifuge (Beckman Coulter, Krefeld, Germany). The gradients were simultaneously fractionated on a Density Gradient Fractionation System (#621140007 ...
-
bioRxiv - Biochemistry 2022Quote: ... Average fluorescence intensity of DCF from 3×105 cells was recorded and data acquisition and analysis were carried out using Summit 5.2 software (Beckman-Coulter, Inc. USA). Morphology of algal cells was examined and photographed with a differential interference contrast microscopy (DIC ...
-
bioRxiv - Cell Biology 2022Quote: ... The gradient was formed by sequentially adding each 3 ml layer to a 14 × 89 mm ultra-clear centrifuge tube (Beckman Coulter, Inc.), starting with the 40% solution and ending with the 5% solution ...
-
bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 16000 ×g for 3 hours at 4 °C in an Avanti J-25 centrifuge (Beckman Coulter Life Sciences, USA) with a JA-25.50 fixed-angle aluminum rotor (Beckman Coulter Life Sciences ...
-
bioRxiv - Molecular Biology 2021Quote: ... sucrose gradients prepared with lysis buffer without Triton X100 and centrifuged at 32,000 rpm for 3 hrs at 4 °C in an SW41Ti rotor (Beckman Coulter, Cat:331362). The sucrose gradients were fractionated using BR-188 Density Gradient Fractionation System with 60% (m/v ...
-
bioRxiv - Zoology 2021Quote: ... Food concentration was maintained at 1500 μm3 mL−1 of phytoplankton cells at the outlet of the tank – ad libitum (Rico-Villa et al., 2009) – and controlled twice daily using an electronic particle counter (Multisizer 3, Beckman Coulter, USA) equipped with a 100 μm aperture tube ...
-
bioRxiv - Neuroscience 2022Quote: ... and a 200-μl aliquot of the final supernatant was added to 3 mL of scintillation fluid and the radioactivity was measured by a liquid scintillation counter (Beckman LS 6000SC). Blank values were obtained by performing the reaction in the absence of tissue homogenate and in the presence of boiled homogenate ...
-
bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
-
bioRxiv - Cell Biology 2024Quote: ... conditioned media were subjected to 10 min at 200g for 3 cycles (G146D centrifuge, Gelec®) and 20 min at 2000g (Allegra 64R Centrifuge, Beckman Coulter) to remove cellular debris and apoptotic bodies respectively ...
-
bioRxiv - Biochemistry 2024Quote: 3 L of over-expressed cells were harvested immediately by centrifugation (Beckman Coulter Avanti J-20 XPI centrifuge, Beckman JLA-8.100 rotor) at 6,000 x g for 10 min at 4 °C ...
-
bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Ribosome Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Ribosome Buffer containing 100 μg/mL cycloheximide in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
-
bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
-
bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min. at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
-
An anti-amyloidogenic treatment to specifically block the consolidation of traumatic events in mousebioRxiv - Animal Behavior and Cognition 2020Quote: ... the rest of the volume was centrifuged for 5 minutes at 4,000 rpm (Microfugue 18 centrifuge, Beckman Coulter) to obtain the cell debris fraction (P1 ...
-
bioRxiv - Neuroscience 2021Quote: ... KG, Staufen, Germany) (position 6, 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge ...
-
bioRxiv - Biochemistry 2021Quote: ... The labeled peptides were further purified by C-18 (5 μm, 4.6 mm × 150 mm, Beckman Coulter Inc.) reverse phase-high performance liquid chromatography ...
-
bioRxiv - Biochemistry 2020Quote: ... After 20 minutes the cells were collected by centrifugation (8,000 rpm, 5 minutes, JLA-16.25 Beckman Coulter rotor), dissolved in 0.5 mL of HEPES:Polymix buffer (5 mM Mg(OAc)2 ...
-
bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min. at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 mM Mg(OAc)2 and 150 mM KCl) and centrifuged at 116,000 xg for 5 h at 4°C in a TL100.3 rotor (Beckman). The ribosome pellet was resuspended by orbital shaking (1 h at 4°C ...
-
bioRxiv - Genetics 2021Quote: ... Reactions were then pooled in a 5 ml tube and purified using a 0.7x SPRIselect (Beckman Coulter B23318) cleanup and eluted in 450μL of TE ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... and washed in 5 volumes of chloroplast wash buffer (8 °C, manual stop when reaching 3000 RPM, Beckman JA-20 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Dialyzed nucleosomes were purified in a 5-30% w/v sucrose gradient with a SW41 Ti (Beckman Coulter) rotor in an Optima L-90K ultracentrifuge (Beckman Coulter ...
-
bioRxiv - Cell Biology 2024Quote: ... 600 µL of the lysate were transferred to an ultracentrifuge tube (5 x 41 mm, Beckman-Coulter, #344090) and centrifuged in an MLS-50 rotor (Beckman-Coulter ...
-
bioRxiv - Genomics 2024Quote: ... by centrifuging the chip facing downwards at 3,220 g for 5 min at 4 °C and purified with 1.5 × Ampure XP beads (Beckman), followed by DNA QC and trace checking.
-
bioRxiv - Genetics 2024Quote: ... followed by inactivation at 85° for 5 min and cDNA purification with AMPure XP beads (Beckman Coulter, A63880) at a 1:1.1 sample-to-bead ratio ...
-
bioRxiv - Biophysics 2022Quote: ... The fractions containing monomeric peptide were pooled and centrifuged at 386000 g for 3 h in an Optima TLX ultracentrifuge equipped with a TLA 120.1 rotor (Beckman Coulter, Atlanta, GA, USA). Orthophosphoric acid or sodium phosphate were added to a final concentration of 20 mM to adjust the pH to 2.8 or 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: The absorbance spectra of recombinant DosS and DosT (3 µM) were determined at room temperature using quartz cuvettes with rubber stoppers in a DU800 spectrophotometer (Beckman Coulter, Fullerton, CA). The reduction of recombinant DosS was achieved via addition of sodium dithionite (DTH ...
-
bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
-
bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
-
bioRxiv - Microbiology 2024Quote: ... aliquots of the cultures were fixed in 70% ethanol to analyze cell mass using the Multisizer 3 Coulter counter (Beckman Coulter, Brea, CA). The remaining cultures were further incubated for 4 h with 0.3 mg/mL rifampicin and 0.01 mg/mL cephalexin to allow run-out replication of chromosomal DNA ...
-
bioRxiv - Microbiology 2024Quote: The virus solutions were centrifuged at 106800 × g for 3 hours at 4 °C using a SW32Ti rotor (Beckman Coulter, Fullerton, CA, USA). The virus pellets were suspended in phosphate-buffered saline (PBS) ...
-
bioRxiv - Cancer Biology 2020Quote: ... CD14 BV605 (1:100) and CD11b PC5 (1:100, Beckman Coulter). Cells were analyzed on the FACSCelesta (BD).
-
bioRxiv - Systems Biology 2022Quote: ... and purification with Ampure XP beads (1:1 ratio, Beckman Coulter) produced a purified cDNA library ...
-
bioRxiv - Genomics 2020Quote: ... cleaned up with 1:1 AMPureXP beads (Beckman Coulter, Brea, CA). ...