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Citations for Beckman :
301 - 350 of 1615 citations for 2' 5' Dichloro 3 3 4 dimethylphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 4°C at 31,000 rpm for 2 h and 30 min using an SW 32 Ti rotor(Beckman Coulter, 369694). The gradients were fractionated using a Teledyne ISCO Density Gradient Fractionation System and 12 fractions were collected ...
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bioRxiv - Immunology 2021Quote: ... Samples were spun at 76,650 x g for 2 hours at 4° C inside a SW 32i rotor using an Optima L-90K Ultracentrifuge (Beckman Coulter). Purified virus was quantified using a bicinchoninic acid assay (BCA ...
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bioRxiv - Genomics 2021Quote: ... The samples were then centrifuged at 28,600 rpm for 2 h at 4°C (Beckman Optima XE-90; SW32 Ti rotor). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
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bioRxiv - Neuroscience 2022Quote: ... layers F1/2 were removed before transferring the combined F3/4 layer into a clean 25mL centrifuge tube (Beckman Coulter, #355618) and diluting in GM buffer (at least 1:4 ...
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bioRxiv - Cell Biology 2024Quote: ... to a volume of ∼12 mL with PBS and separated by ultracentrifugation at 160.000 × g for 2 h (4 °C) in a SW Ti41 rotor (Beckman Coulter #331362). Top-layer LDs were collected by aspiration using a pre-wetted 200 µL pipette tip so that maximal LDs were collected in minimal buffer volume ...
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bioRxiv - Molecular Biology 2024Quote: ... lysed by sonication and ultracentrifuged at 26K rpm at 4 °C for 2 h (using optima XPNs Ultra Centrifuge, Beckman Coulter). The clarified lysate was loaded onto pre-equilibrated Ni-NTA column ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 26K RPM at 4 °C for 2 h (using optima XPNs Ultra Centrifuge, Beckman Coulter). Cell-free extract so prepared was loaded onto 1 ml pre-equilibrated Ni-NTA column ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was subjected to ultracentrifugation at 26K rpm at 4 °C for 2 h (using optima XPNs Ultra Centrifuge, Beckman Coulter). Cell lysate was loaded onto 1 ml pre-equilibrated Ni-NTA column ...
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bioRxiv - Microbiology 2024Quote: ... Particles from the filtrate were concentrated by ultracentrifugation at 100,000 x g for 2 h at 4°C using an SW32Ti rotor in an Optima LE-80K centrifuge (Beckman Coulter). The pellets from different centrifuge tubes were pooled and resuspended in 1 mL of 1X PBS and pelleted again by ultracentrifugation at 165,700 x g for 2 h at 4°C in a TLA-55 rotor (Beckman Coulter) ...
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bioRxiv - Genomics 2024Quote: ... We then pelleted this concentrate by centrifugation in a swinging bucket centrifuge (25,000g, 2 hours, 4°, Beckman Coulter SW 32 Ti) and resuspended it in cold PBS + 4% glucose ...
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bioRxiv - Biophysics 2024Quote: ... SV experiments were performed at 20°C by UV intensity detection using a 4-hole An-60 Ti rotor and standard 2-channel 12-mm Epon charcoal-filled centerpieces (Beckman Coulter). Samples were run at 26,000 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were ultracentrifuged at 120,000 to 140,000 x g (SW-28 rotor) for 2 hours at 4°C to pellet EVs on an OptimaTM L-100 XP ultracentrifuge (Beckman Coulter). The supernatant was removed ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM Mg(OAc)2) and centrifuged for 20 min at 100,000 x g in a 70Ti rotor (Beckman Coulter). The resulting membrane pellet was resuspended in 1 ml rough microsome (RM ...
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bioRxiv - Genomics 2021Quote: ... The spores were washed twice with sterile ddH2O and pelleted at 3700g for 5 min at 4 °C in an Allegra X-12R centrifuge (Beckman, Brea, CA, USA). Spores were resuspended in sterile ddH2O and kept at 4°C until experiments.
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bioRxiv - Microbiology 2022Quote: ... then clarified by centrifugation at 2000 rpm at 4°C for 5 min in a refrigerated tabletop centrifuge (Beckman Coulter, Indianapolis IN, USA) to remove large debris ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was collected and treated with polyethyleneimine to a final concentration of 0.03% incubated on ice for 5 minutes and subsequently centrifuged at 10,000 rpm at 4°C for 10 minutes (Beckman coulter, JA-25.50 rotor). The supernatant was collected and powdered ammonium sulfate (approx 15 g ...
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bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 130,000 × g for 2 h at 4°C in a TLA100.3 rotor (Beckman Optima TLX table top centrifuge). Following centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... The post nuclear supernatant was transferred to an ultracentrifuge tube and centrifuged for 2 h at 245,000 g at 4 °C in a Beckman L90 Ultracentrifuge (Beckman Coulter, Mississauga, Canada) to pellet remaining membrane.
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bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Cat no UZ-PA-38.5-1) at 100,000 g at 4 °C for 2 h in a swinging-bucket rotor (Beckman Coulter; SW28 or SW32).
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bioRxiv - Immunology 2022Quote: ... Ultracentrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2 hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, CA, USA) was performed to pellet the viruses through the sucrose cushion while soluble egg proteins were removed ...
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...
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bioRxiv - Plant Biology 2023Quote: ... 700 μl of obtained extract was applied to a chilled sucrose density gradient and centrifuged at 4 °C for 2 hours 45 minutes at 151,000 ×g using the SW 41 Ti rotor (Beckman Culture Life Sciences). After centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were loaded onto the sucrose gradients and centrifuged at 40’000 rpm for 2 h at 4°C using the SW 41 Ti rotor (Beckman Coulter, Cat# 331362). Subsequently ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Microbiology 2024Quote: ... EMVs were obtained by ultracentrifugation from 3 mL of filtrate at 100,000 × g (average centrifugal force) and 4 °C for 2 h with 50Ti rotor and Optimax centrifuge (Beckman Coulter, Brea, CA, United States). The supernatant after removing EMVs was collected as PVF ...
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bioRxiv - Molecular Biology 2022Quote: ... 4°C (Beckman Coulter). The pellet containing lentiviral particles was suspended in the residual medium ...
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bioRxiv - Genomics 2020Quote: ... 4°C (Beckman Coulter). Supernatant was loaded onto 5 ml HisTrap FF columns (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... 4°C (Beckman Coulter). Clarified lysates were poured several times over 0.5-1 ml Streptactin resin (IBA ...
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bioRxiv - Cancer Biology 2023Quote: ... 4°C (Beckman Coulter). The pellet containing lentiviral particles was suspended in the residual medium ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Microbiology 2024Quote: ... Virus was collected 48 hours post-transfection and pelleted over a 10% sucrose cushion at 25,000 RPM for 2 hours at 4 °C using a SW32Ti rotor (Beckman Coulter Life Sciences, Brea, CA, USA). Pellets were resuspended in DMEM (Gibco ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... A Multisizer 4 (Beckman Coulter) machine was used ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (SW55Ti rotor, Beckman). The pellets were resuspended in 25% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (VTi65.1 rotor, Beckman) with the maximum acceleration and minimum deceleration allowing for isopycnic separation of subcellular particles and membranes ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C (SW41 rotor, Beckman). The supernatant was concentrated with a 100-kDa molecular weight cutoff concentrator (Merck Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... 4-laser Gallios (Beckman Coulter) flow cytometer ...
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bioRxiv - Physiology 2020Quote: ... one bird per pen in Experiment 2) and centrifuged for 5 min at 17013 × g using a Beckman microfuge (Model E 348720, Beckman Instruments, INC, Palo Alto, CA). Then the viscosity of ileal supernatant was measured using a Brookfield cone-plate digital viscometer (Model LVDV-Ⅲ ...