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Citations for Beckman :
301 - 350 of 1848 citations for 1 Piperidineacetamide 4 2 benzothiazolyl N 4 4 morpholinyl phenyl 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Neuroscience 2023Quote: Monomeric wild-type α-syn was ultracentrifuged at 90,000 RCF for 1 hour at 4 °C to remove any amorphous aggregates (Beckman Coulter Optima Max-XP with MLA-130 rotor) ...
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bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged for 1 h at 100,000 g and 4°C in a TLS 55 rotor (Beckman-Coulter) with the appropriate adapters (#358614) ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifuged at 100,000 rpm (543,000 × g) for 1 h at 4°C with a TLA110 rotor and an Optima MAX-TL ultracentrifuge (Beckman Coulter). Subsequently ...
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bioRxiv - Biophysics 2024Quote: ... the clarified lysate underwent a further centrifugation step at 205,000xg for 1 hour at 4 °C (60Ti rotor, Beckman Coulter). The membrane pellet was then resuspended in the resuspension buffer ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.4) and centrifuged at 182,000g for 1.5 h at 4°C (Beckman SW40). Then the lipid droplets on the top were collected and washed for further analysis.
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bioRxiv - Systems Biology 2020Quote: ... pelleted at 3,709 xg for 4 min (Allegra X-30R, C0650 adaptor, Beckman Coulter), and resuspended in 50 mL of ice-cold deionized water (MilliQ Biocel A10 ...
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bioRxiv - Cell Biology 2021Quote: ... at 4°C using the SW 55 Ti Swinging Bucket rotor (Beckman Coulter, 342194).
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bioRxiv - Molecular Biology 2022Quote: ... After centrifugation at 6000 g for 15 min at 4 °C (JA14 rotor-Beckman), the cell pellet is resuspended with cold lysis buffer (1mg/ml lysozyme ...
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bioRxiv - Cell Biology 2021Quote: ... After centrifugation (55,000 rpm, 80 min, 4°C) in a TLS55 rotor (Beckman Coulter), fractions were collected manually for precipitation with trichloroacetic acid (TCA ...
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bioRxiv - Cell Biology 2021Quote: ... After centrifugation (38,000 rpm, 210 min, 4°C) in an SW41 rotor (Beckman Coulter), RNA content was measured at A254 while fractions were collected using a Foxy Jr ...
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bioRxiv - Biochemistry 2020Quote: ... and spun at 90,000 rpm for 10 min at 4°C (TLA120.1 Beckman Coulter). After incubation at 37°C for 2 mins ...
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 4°C for 30min at 5,000xg and subsequently at 10,000xg (Beckman Coulter Optima XPN-80 with a SW60 rotor) ...
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bioRxiv - Neuroscience 2024Quote: ... for 20 min at 4°C using the pre-cooled 70.1 rotor (Beckman, 342184). Pellets were kept on ice ...
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bioRxiv - Cell Biology 2024Quote: ... and 10,000 g (30 min, 4 °C, SW28 rotor; Optima XPN Ultracentrifuge, Beckman Coulter) to pellet large EVs52 ...
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bioRxiv - Cancer Biology 2024Quote: ... 10,000 × g for 30 min) at 4°C (Beckman Coulter Avanti J-E Centrifuge); filtered through a 0.22 μm PES filter (Genesee) ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was centrifuged at 100,000 × g for 18 h at 4°C (Beckman-Coulter XPN-80 Ultracentrifuge ...
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bioRxiv - Biochemistry 2024Quote: ... and cleared by centrifugation at 4°C (18500 rpm, JA-25.50 Beckman, 20 min,). The clarified extract was purified on a 4 ml His60 Ni-IDA Superflow resin (Ozyme ...
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bioRxiv - Biophysics 2024Quote: Cells were harvested (Beckman F10 BCL, 20 min at 6000 rpm and 4 °C), resuspend in PBS pH 7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by centrifugation at 36,000 rpm for 3 hours at 4°C (Beckman Coulter). RNA was then extracted from the monosomes fraction using Trizol (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Microbiology 2024Quote: ... EMVs were obtained by ultracentrifugation from 3 mL of filtrate at 100,000 × g (average centrifugal force) and 4 °C for 2 h with 50Ti rotor and Optimax centrifuge (Beckman Coulter, Brea, CA, United States). The supernatant after removing EMVs was collected as PVF ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
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bioRxiv - Cell Biology 2024Quote: ... plasma samples were thawed on ice and subjected to ultracentrifugation at 100,000 g for 1 hr or 18 hr at 4 °C (TLA-55 rotor; Optima MAX-MP Tabletop Ultracentrifuge, Beckman Coulter). Pellets were washed once in 1.0 ml PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Bioengineering 2023Quote: ... The suspension was then ultracentrifuged (58,000 × g, 1 hr., 4 °C) in an Optima MAX-XP (Beckman Coulter, Indianapolis, IN, USA) to separate residual catalyst ...
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
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Sense codon-misassociated eRF1 elicits widespread ribosome stalling and induction of quality controlbioRxiv - Molecular Biology 2024Quote: ... The mixture was layered over 900 μl of 1 M sucrose cushion 46 and ultracentrifuged at 100,000 rpm ( 543,000 × g) at 4°C for 1 h using a TLA-110 rotor and an Optima MAX-TL Ultracentrifuge (both Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered through 0.45-μm syringe filters and ultracentrifuged at 100,000 ×g for 1 h at 4 °C (MLA-50 fixed angle rotor, Beckman Coulter, Germany). The supernatant was discarded ...
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bioRxiv - Cell Biology 2020Quote: ... and ultracentrifuged with a 70 Ti rotor (Beckman Coulter; 50,000 rpm, 75 min, 4°C). Supernatants after ultracentrifugation were mixed with COSMOGEL GST-Accept beads (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2021Quote: ... at 40000 rpm for 30 min at 4°C with a Ti45 rotor (Beckman, USA). Mpro-containing supernatant was applied to an immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Neuroscience 2021Quote: ... S1 was centrifuged at 30,000 rpm for 30 min at 4°C (Beckman S4180 rotor). The obtained pellet (P2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 4 mM magnesium acetate) were prepared in 25 × 89 mm polyallomer tubes (Beckman Coulter) using a Gradient Master (BioComp) ...
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bioRxiv - Genomics 2020Quote: ... and separated by ultracentrifugation at 4°C for 3 hours (35300 rpm, Beckman, SW41 rotor) through a 5-50% sucrose gradient (40 mM Tris-HCl pH 8.4 ...
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bioRxiv - Biochemistry 2020Quote: ... the filtered cell culture fluid was ultracentrifuged (25k rpm, 4 °C, SW55Ti Rotor, Beckman Coulter) through a 20% sucrose cushion prepared in TNE buffer (10 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... and concentrated by ultracentrifugation (100,000 × g, 90 min 4 °C, SW41, Beckman Coulter, Brea, CA). The pellet was resuspended in reducing Laemmli SDS-PAGE sample buffer ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation (Beckman Avanti, 45 min, 40,000 x g, 4 °C) and supernatants were diluted 3-fold in 20 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2020Quote: Particle size distribution was determined using a Coulter Multisizer 4 (Beckman Coulter, Inc., Miami, FL). Particles were resuspended in double distilled water and added dropwise to 100 ml of ISOTON II solution until the coincidence of the particles was between 8% and 10% ...
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bioRxiv - Cell Biology 2020Quote: ... and ultracentrifuged with a 70 Ti rotor (Beckman Coulter; 50,000 rpm, 75 min, 4°C). The supernatants were mixed with COSMOGEL GST-Accept beads (Nacalai Tesque ...
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bioRxiv - Bioengineering 2022Quote: ... samples (10 mL) were first centrifuged at 7000Xg at 4°C for 30 minutes (Beckman Coulter Avanti J-E Series ...
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bioRxiv - Biochemistry 2021Quote: ... The bacterial culture was centrifuged at 4,000 × g for 15 min at 4°C (Beckman) and the cell pellet was resuspended in 30-40 mL of lysis buffer (50 mM HEPES pH 8.0 ...