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Citations for Beckman :
301 - 350 of 1136 citations for 1 Butylpyrrolidin 1 ium 3 yl 2 cyclopentyl 2 hydroxy 2 phenylacetatechloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
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bioRxiv - Microbiology 2024Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each compound was dissolved in DMSO at a concentration of 2 mM and distributed into an Echo Qualified 384-Well Polypropylene Microplates (Beckman Coulter, 001-14615). Using an Echo 655 Liquid Handler ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
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bioRxiv - Microbiology 2024Quote: ... EMVs were obtained by ultracentrifugation from 3 mL of filtrate at 100,000 × g (average centrifugal force) and 4 °C for 2 h with 50Ti rotor and Optimax centrifuge (Beckman Coulter, Brea, CA, United States). The supernatant after removing EMVs was collected as PVF ...
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bioRxiv - Cancer Biology 2020Quote: ... Filtered media containing lentiviral particles were centrifuged at 24000rpm at 20°C for 2 hrs in 90Ti rotor in ultra-centrifuge (Beckman Coulter Optima TM L-100K). Viral particle pellet was obtained after discarding supernatant post centrifugation and suspended in 100 μl OptiMEM media for overnight at 4°C.The collected lentivirus suspension was used for transfection or stored for later use at −80°C ...
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bioRxiv - Physiology 2020Quote: ... one bird per pen in Experiment 2) and centrifuged for 5 min at 17013 × g using a Beckman microfuge (Model E 348720, Beckman Instruments, INC, Palo Alto, CA). Then the viscosity of ileal supernatant was measured using a Brookfield cone-plate digital viscometer (Model LVDV-Ⅲ ...
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bioRxiv - Microbiology 2024Quote: ... Viruses were collected 48 hours post-transfection and pelleted in DPBS over a 10% sucrose cushion at 25,000 RPM for 2 hours using a SW32Ti rotor (Beckman Coulter Life Sciences, Brea, CA, USA). Virus pellet was then resuspended in labeling buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2024Quote: ... Virus was collected 48 hours post-transfection and pelleted over a 10% sucrose cushion at 25,000 RPM for 2 hours at 4 °C using a SW32Ti rotor (Beckman Coulter Life Sciences, Brea, CA, USA). Pellets were resuspended in DMEM (Gibco ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... the genomic DNA was cleaned up with AMPure XP beads at 3:1 ratio (Beckman Coulter A63880) and eluted in the 10 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).
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bioRxiv - Cell Biology 2020Quote: ... containing the phosphatases and proteases inhibitors and centrifuged at 100,000xg for 1 h through a 3 ml 1.4 M sucrose cushion using a TL100 centrifuge (Beckman). The crude heavy nuclear fraction and purified nuclei were resuspended in buffer A and extracted with 1% Triton X-100 for 5 min at 4°C (cNM ...
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bioRxiv - Systems Biology 2024Quote: The stained 1 in 3 cell solution was assessed by flow cytometry (CytoFlex S Flow Cytometer, Beckman Coulter) and the number of singlet (based on FSC-H vs FSC-A) ...
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bioRxiv - Plant Biology 2024Quote: ... Cultures containing 1-3 x 106 cells/mL were collected at 1,670 xg (JLA-10.500 rotor, Beckman Coulter). Cells were washed once (D ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were separated through ultracentrifugation for 1 hr at 200,000 g (spin 3 at 49,000 r.p.m. in Beckman TLS-55 rotor). Top layers of supernatants were removed ...
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bioRxiv - Neuroscience 2024Quote: ... at a ratio of 1:2.3 before layering on 3 ml of SCB in a 12 ml Thinwall Ultra-clear centrifuge tubes (Beckman Coulter). Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Cell Biology 2020Quote: ... A 1:1 Ampure XP (Beckman Coulter) PCR purification step was performed followed by assessment of size distribution using an Agilent high-sensitivity DNA chip (Agilent technologies ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then enriched in DNA sizes between 200 and 600 bp (corresponding to 1 to 3 nucleosomes) using AMPure XP magnetic beads (Beckman-Coulter), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
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bioRxiv - Immunology 2024Quote: ... PD-1 (PE-Cy5.5, 1/320, Beckman Coulter). Staining was performed in 1X PBS with 5% FBS and incubated in the dark for 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... PD-1 (PE-Cy5.5, 1/320, Beckman Coulter). Staining was performed in 1X PBS with 5% FBS and incubated in the dark for 30 minutes ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Cancer Biology 2020Quote: ... CD14 BV605 (1:100) and CD11b PC5 (1:100, Beckman Coulter). Cells were analyzed on the FACSCelesta (BD).
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bioRxiv - Systems Biology 2022Quote: ... and purification with Ampure XP beads (1:1 ratio, Beckman Coulter) produced a purified cDNA library ...
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bioRxiv - Genomics 2020Quote: ... cleaned up with 1:1 AMPureXP beads (Beckman Coulter, Brea, CA). ...
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bioRxiv - Plant Biology 2020Quote: ... purified with the double AMPure XP cleanup (1:1) (Beckman Coulter), and checked with a High sensitivity DNA chip (Agilent technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... was removed using right-side size selection with AMPure XP beads at a 1:1 and 7:1 bead:sample ratio (Beckman Coulter) described previously26 ...
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bioRxiv - Systems Biology 2024Quote: ... After cleanup with AMPure XP Reagent (Beckman Coulter, 1:1 ratio beads:PCR), iPCR amplification was carried out with KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using 1:1 (vol: vol) AMPure XL beads (Beckman Coulter). cDNA prepared as above was end-prepped using NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB ...
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bioRxiv - Genomics 2021Quote: ... then re-purified with SPRI beads with 1:1 ratio (Beckman Coulter, B23317), and eluted in 25 µl of water (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... HIV-1 p24 was stained with Anti-HIV-1 p24-PE (KC57) (Beckman Coulter) for 30 min on ice and washed again 3 times before measuring on the BD FACSCelesta™.
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bioRxiv - Microbiology 2021Quote: ... using anti-HIV-1 p24-PE antibody (#6604667, 1:1000 dilution) purchased from Beckman Coulter ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was purified using a 1:1 volumetric ratio of Ampure Beads (Beckman Coulter) and eluted in 20 ml of water ...
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bioRxiv - Genomics 2023Quote: ... The samples were further purified using Ampure XP (1:1) (A63882, Beckman Coulter, US) and diluted in 10 µl elution buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was purified using a 1:1 volumetric ratio of Ampure Beads (Beckman Coulter) and eluted in 20 ml of water ...
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bioRxiv - Cancer Biology 2023Quote: ... 7AAD (1:100; Beckman Coulter) was used for dead cell exclusion ...
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bioRxiv - Cancer Biology 2023Quote: ... 7AAD (1:100; Beckman Coulter) was used ...
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bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1) ...
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bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1).