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Citations for Beckman :
301 - 350 of 1366 citations for 1 5 Bromo 1 2 Trimethylsilyl Ethoxy Methyl 1H Pyrrolo 2 3 B Pyridin 3 Yl Ethanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: 3 L of over-expressed cells were harvested immediately by centrifugation (Beckman Coulter Avanti J-20 XPI centrifuge, Beckman JLA-8.100 rotor) at 6,000 x g for 10 min at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Ribosome Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Ribosome Buffer containing 100 μg/mL cycloheximide in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Microbiology 2020Quote: ... the 100,000xg centrifugation was performed for 1h at 4°C using a SW41 TI swinging bucket rotor (Beckman Coulter). The pellet was then resuspended in 50 μL or 1500 μL of D-MEM for subsequent analysis.
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bioRxiv - Cell Biology 2020Quote: ... Fractions were diluted and washed in PBS for 1h at 100,000 x g in a TLA 110 rotor (Beckman). Fractions were resuspended in 35 ul of PBS ...
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bioRxiv - Biophysics 2021Quote: ... 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
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bioRxiv - Developmental Biology 2023Quote: ... the supernatant was centrifuged in a TLA100.4 fixed angle rotor at 100000 g for 1h at 37°C in an Optima TL ultracentrifuge (Beckman). The supernatant containing globular actin was collected ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: ... CD103 (APC, B-Ly7, eBioscience, or biotin, 2G5, Beckman Coulter ...
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bioRxiv - Immunology 2024Quote: ... and DuraClone IM B cell tubes (#B53318, Beckman Coulter) respectively ...
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bioRxiv - Cell Biology 2022Quote: ... The product from PCR-2 was purified using 1x volume of AMPure XP beads (Beckman Coulter) and eluted in 15 μL of IDTE buffer (IDT DNA) ...
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bioRxiv - Cell Biology 2021Quote: ... After centrifugation (285, 000 g for 2 h at 4 °C using a Beckman SW40Ti rotor), polysome profiles were recorded using an Äktaprime plus chromatography system (GE Healthcare) ...
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bioRxiv - Bioengineering 2021Quote: ... The supernatant was then ultracentrifuged for 2 hours using Optima™ L-80XP Ultracentrifuge (Beckman Coulter) at 100,000 x g at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The solution was centrifuged at 36,000 rpm for 2 h in a SW41Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 4°C for 2 h (SW 32 Ti swinging-bucket rotor, Optima LE-80K Beckman Coulter) using a 25% w/v sucrose cushion ...
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bioRxiv - Genetics 2021Quote: ... We purified the final libraries with 2 rounds of 0.8X volume of SPRISelect (Beckman Coulter, B23318).
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bioRxiv - Microbiology 2021Quote: ... and centrifuged for 2 h at 64,000 g in A27-8×50 mL rotors (Beckman Coulter) to sediment virus particles ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volume (2 ml i.e 1600 µg) of cell extracts were loaded in ultracentrifuge tubes (Beckman). Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman) ...
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bioRxiv - Bioengineering 2023Quote: ... in 2%BSA and 2mM EDTA and sorted using FACS sorter (Beckman Coulter, MoFlo Astrios Zellsortierer). For gating ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 2% BSB-PFA and analysed by Flow Cytometry on BD Celesta instrument (Beckman Coulter) using FlowJoTM software ...
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bioRxiv - Immunology 2023Quote: Cells were washed with 2% FACs and data was acquired using CytoFLEX S (Beckman Coulter, USA) and analyzed via FlowJo v10 software (Oregon ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 35,000 rpm for 2 hrs 40 min using a SW41Ti rotor (Beckman). Gradients were fractionated using a Brandel (UV ...
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bioRxiv - Genetics 2024Quote: ... The filtered medium was centrifuged at 19400 rpm and 17 °C for 2 h (Beckman Coulter). The pellet was suspended in 250 µL HBSS (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... EVs were pelleted by ultracentrifugation at 55,000 × rpm for 2 hours using the TLA120.4 rotor (Beckman). The EV pellet was resuspended in 1XPBS and frozen at −80 °C until further analysis.
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bioRxiv - Cancer Biology 2024Quote: ... then trypsinized and resuspended in PBS with 2% FBS for quantification using a flow cytometer (Beckman). JC-1 monomers and aggregates were detected at PE and FITC channels ...
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bioRxiv - Biophysics 2022Quote: ... The fractions containing monomeric peptide were pooled and centrifuged at 386000 g for 3 h in an Optima TLX ultracentrifuge equipped with a TLA 120.1 rotor (Beckman Coulter, Atlanta, GA, USA). Orthophosphoric acid or sodium phosphate were added to a final concentration of 20 mM to adjust the pH to 2.8 or 7.4 ...
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bioRxiv - Biochemistry 2021Quote: The absorbance spectra of recombinant DosS and DosT (3 µM) were determined at room temperature using quartz cuvettes with rubber stoppers in a DU800 spectrophotometer (Beckman Coulter, Fullerton, CA). The reduction of recombinant DosS was achieved via addition of sodium dithionite (DTH ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
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bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of the cultures were fixed in 70% ethanol to analyze cell mass using the Multisizer 3 Coulter counter (Beckman Coulter, Brea, CA). The remaining cultures were further incubated for 4 h with 0.3 mg/mL rifampicin and 0.01 mg/mL cephalexin to allow run-out replication of chromosomal DNA ...
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bioRxiv - Microbiology 2024Quote: The virus solutions were centrifuged at 106800 × g for 3 hours at 4 °C using a SW32Ti rotor (Beckman Coulter, Fullerton, CA, USA). The virus pellets were suspended in phosphate-buffered saline (PBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was washed with PBS and ultracentrifuged at 100,000 × g 1h at 4°C to obtain small vesicles (TL-100 Ultracentrifuge, Beckman Coulter). The final pellet was resuspended in PBS for subsequent experiments ...
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bioRxiv - Biochemistry 2020Quote: ... the crude mitochondria were undergone a sucrose gradient in SEM buffer for 1h at 139,400 x g with an SW40 Ti rotor (Beckman Coulter). Mitochondrial band was pooled and stored at −80 °C.
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bioRxiv - Microbiology 2021Quote: ... The membrane fraction was prepared using ultracentrifugation (45,000 rpm, 4 °C, 1h; Optima-90 ultracentrifuge, Beckman-Coulter, Brea, CA, USA) and washed once in 200 mM ABC buffer ...
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bioRxiv - Microbiology 2021Quote: ... The resting supernatants were filtered through 0.45µm syringe filters and ultracentrifuged at 100,000 x g for 1h at 4°C (SW41 Ti swinging-bucket rotor, Beckman Coulter). The supernatant was discarded and the pellet suspended in 0.22 µm-pore filtered or 0.02 µm-pore filtered (for Flow Cytometry analysis ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter, 369650; Optima XE, Beckman Coulter). Phagosome-containing beads float at the interface between the 10% and 25% layers ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Cancer Biology 2020Quote: ... CD14 BV605 (1:100) and CD11b PC5 (1:100, Beckman Coulter). Cells were analyzed on the FACSCelesta (BD).
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bioRxiv - Systems Biology 2022Quote: ... and purification with Ampure XP beads (1:1 ratio, Beckman Coulter) produced a purified cDNA library ...
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bioRxiv - Genomics 2020Quote: ... cleaned up with 1:1 AMPureXP beads (Beckman Coulter, Brea, CA). ...
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bioRxiv - Plant Biology 2020Quote: ... purified with the double AMPure XP cleanup (1:1) (Beckman Coulter), and checked with a High sensitivity DNA chip (Agilent technologies) ...
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bioRxiv - Biochemistry 2023Quote: ... Colorimetric changes was measured 3 min after mixing the conditioned media with DTNB solution at 450 nm of absorbance (DTX880, Beckman Coulter, Indianapolis, IN, USA). The value from DTNB only was substracted as noise ...
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bioRxiv - Bioengineering 2024Quote: ... using C4F10-saturated PBS until the subnatant was clear after which MBs were counted using a Coulter Counter Multisizer 3 (50 µm aperture tube, Beckman Coulter, Mijdrecht, the Netherlands). Next ...
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bioRxiv - Immunology 2023Quote: ... and B cells were determined using Flow-Count fluorospheres (Beckman) and a Cytoflex flow cytometer (Beckman ...
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bioRxiv - Neuroscience 2020Quote: ... and were centrifuged at 82,500 x g for 2 h at 4 °C (Beckman SW28 swing rotor). The band between 1.0 and 1.2 M sucrose was collected as the synaptosome fraction and diluted with 80 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Pathology 2021Quote: ... BALF (10 μl) was applied to glass microscopy slides using a cytocentrifuge (StatSpin CytoFuge 2, Beckman Coulter). Slides were fixed with 10% neutral buffered formalin ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in 67 mM HEPES-KOH pH 7.2) after 2 h of ultracentrifugation (4 °C, 5000 RPM, Beckman SW41 ...
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bioRxiv - Biochemistry 2023Quote: ... The cell culture microplate was centrifuged at 300g for 2 min (Beckman Coulter Allegra X-22R Centrifuge) to bring the cells to the well bottom ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated for 2 hr at 60°C and purified by Ampure XP beads (Beckman #A63881). Libraries were PCR amplified in a 30 µL reaction containing 14 µL circularized library ...