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Citations for Beckman :
251 - 280 of 280 citations for Zika Virus Lysate PRVABC59 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The amount of radioactivity in the cell lysate was measured using a liquid scintillation counter (Beckman Coulter, Brea, CA, USA) and protein concentration was determined via the Lowry method54 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were cleared by centrifugation for 30 min at 4°C at 18,000 rpm (SS-34 rotor; Beckman-Coulter, USA).
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bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... Genetic material was pulled down and purified by mixing the lysate in each well with 2.2x volumes of Agencourt RNAClean XP SPRI beads (Beckman Coulter) and washing 3x with 75 μL of 80% ethanol ...
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bioRxiv - Neuroscience 2023Quote: ... the crude lysate was subjected to iodixanol gradient (15%, 25%, 40% and 60%) ultracentrifugation using a Type 70Ti rotor (Beckman) at 490000 × g ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 40,000 rpm for 35 min at 4°C (Type 45 Ti Rotor, Beckman Coulter). The resulting supernatant was mixed with 1ml pre-washed IgG Sepharose 6 FastFlow beads (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... Then this clear cell lysate was loaded on top the gradient and ultracentrifugation was done for 5hrs at 133,000g on SW60Ti (Beckman Coulter) rotor to separate each gradient ...
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bioRxiv - Biochemistry 2023Quote: ... by dounce homogenization (20 strokes).The lysate was clarified by centrifugation at 56,000 rpm in a Type 70 Ti rotor (Beckman Coulter) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed by sonication and lysate was cleared by spinning down in a Ti70 at 65K rpm (4ºC) for 30 min (Beckman Coulter). Cleared lysate was briefly incubated (approximately 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were then loaded onto a 5-50% sucrose density gradient and centrifuged in an SW41 Ti rotor (Beckman) at 36,000 rpm for 2 h at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... From each sample 100 µl lysate were transferred to a special tube for ultra-centrifugation (Beckman Coulter, 1.5 ml tube, 257448). The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80 ...
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bioRxiv - Microbiology 2021Quote: ... The remaining lysate was layered on top of a 30% (w/w) sucrose cushion and centrifuged using a Beckman SW41 rotor (Beckman Coulter) at 32,000rpm and 4°C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal optical density units from cleared cell lysates were loaded onto the gradient and centrifuged in a SW40 rotor (Beckman Coulter) for 5 hr ...
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bioRxiv - Biochemistry 2023Quote: ... and the lysates were centrifuged at 100,000 ×g for 30 min in a Beckman 70.1Ti rotor (Beckman Coulter, CA, USA, #342184). The supernatant was loaded onto a DEAE Sepharose Fast Flow column (Cytiva ...
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bioRxiv - Microbiology 2023Quote: 50 mL of freshly prepared phage lysate (> 108 pfu/mL) was ultracentrifuged (Optima XE-100 ultracentrifuge, Beckman Coulter, Brea, CA, USA) at 109 ...
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bioRxiv - Biochemistry 2023Quote: ... The brains were homogenised using an Ultra-Turrax® blender and the lysates cleared by centrifugation at 112,000 ×g in TLA-55 fixed-angle rotor (Beckman Coulter) for 30 min at 4°C (i.e ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of the cell lysate in each well were transferred to a Proxi plate by a Beckman NX (Beckman Coulter). The plates were then promptly de-ionized (to prevent ESI spray instability ...
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bioRxiv - Biophysics 2020Quote: ... The lysates were clarified by centrifugation (16,000 rpm for 1 h at 4°C, rotor JA 25.50, Beckman Coulter, Brea, California, USA). The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad ...
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bioRxiv - Biochemistry 2020Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 g for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was then applied on a continuous 7-47% sucrose gradient (mol weight/volume) in ultra clear tubes (Beckman & Coulter, #331372) and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter ...
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bioRxiv - Neuroscience 2020Quote: ... The lysate was cleared by centrifugation at 30,000 g for 30 minutes in a JA 25.50 rotor (Beckman Coulter, Brea, CA, USA). Streptomycin sulfate was added to the lysate at 10 mg/ml to precipitate DNA ...
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bioRxiv - Biochemistry 2021Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... The lysate was cleared by centrifugation at 30,000 g for 30 minutes in an JA 25.50 rotor (Beckman Coulter, Brea, CA, USA). The His-tagged target protein was purified by Ni-NTA gravity-flow chromatography (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and the resultant lysate was transferred into SS34 tubes for further centrifugation at 18,000 rpm (JA-25.50 rotor, Beckman Coulter Avanti J-E) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifugation at 5500 rpm for 20 min at 4°C (AllegraTM 21R Centrifuge, Beckman Coulter, CA, USA). The sample was then loaded into a HisTrap HP 5 mL column (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... Gradients were then chilled on ice for 30 minutes prior to loading lysate and centrifugation at 40,000 rpm on a TLS-55 swinging bucket rotor (Beckman Coulter, Brea, CA) for 16 hours at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... the cell lysates are loaded to the gradients of iodixanol (15%, 25%, 40% and 60%) in the OptiSeal tubes (Beckman Coulter, Indianapolis, IN). Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... for 10 m at 4°C and pelleted the lysates at 50,000×g for 1 h at 4°C using an Optima MAX-XP ultracentrifuge (Beckman Coulter, Brea, CA, USA). We resuspended cell fractions in 500 μL water and stored the samples at 4°C until analysis ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were collected and RNA was isolated using the Biomek FxP and the RNAdvance Tissue Isolation kit (Beckman Coulter, USA, p/n A32646). The resulting RNA was quantified using the Nandrop 8000 (Nanodrop ...
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bioRxiv - Biochemistry 2019Quote: ... Resuspended cells were lysed by French Press (8,000 psi, 4°C, 2 passages) and lysates were centrifuged (Beckman 60Ti rotor, 30 min, 50,000 rpm, 4°C). Pellets were resuspended in 100 ml of buffer B (PBS [140 mM NaCl ...