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Citations for Beckman :
251 - 300 of 471 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 10 μl of each replicate amplicon were pooled and purified using 1V AmPure XP beads (Beckman Coulter) and used as a template for the second PCR ...
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bioRxiv - Cell Biology 2023Quote: ... 10% of the sorted cells were used to measure cell size using a Z2 Coulter counter (Beckman), and the rest of the cells were lysed in RIPA lysis buffer supplemented with protease inhibitors and phosphatase inhibitors ...
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bioRxiv - Microbiology 2024Quote: ... A 10–µl aliquot of each sample was then aspired on a CytoFLEX Flow Cytometer (Beckman Coulter) with 10 µl min−1 flow rate ...
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bioRxiv - Biochemistry 2024Quote: ... 10 mM Tris-HCl pH 7.5)] in an ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter) at 40,000 rpm (185,511 × g ...
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bioRxiv - Developmental Biology 2024Quote: ... Lentivirus containing > 10 kb length insert (inducible CRISPRi) was concentrated using AVANTI J-30I centrifuge (Beckman Coulter) with JS-24.38 swing rotor at 100,000g for two hours at 4°C and pellets were dissolved in 200 μL PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... The amplified DNA library was purified by mixing 10 µL AMPure XP beads (Beckman Coulter, Miami, FL, USA) according to the manufacturer’s protocol and eluted using 10 µL nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: ... 10 mM Tris-HCl in DEPC-treated water) in a thick-walled ultracentrifuge tube (Beckman Coulter, Cat # 355631) and spun at 107,000 RCF ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were broken by sonication and centrifuged at 8,000 rpm for 10 min with JA25.5 rotor (Beckman) to remove cell debris ...
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bioRxiv - Biochemistry 2020Quote: ... before being pelleted in a TLA120.1 rotor at 90,000 rpm for 10 min at 25°C (Beckman Coulter) (Subramanian et al. ...
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bioRxiv - Microbiology 2020Quote: ... Then 4 ml of the adjusted lipoprotein was layered under 8 ml of 0.9% NaCl and centrifuged at 49,000 rpm for 4 hours at 10°C (NVTi 65 rotor; Beckman). The HDL (lower band ...
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bioRxiv - Microbiology 2020Quote: ... sucrose cushions and subjected to ultracentrifugation (90,000 r.p.m. for 10 minutes) using an Optima TLX Ultracentrifuge (Beckman Coulter). Pelleted virions were resuspended in SDS-sample buffer and subjected to immunoblot analysis using an anti-SARS-CoV-2 spike mouse monoclonal antibody (1:1,000 ...
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bioRxiv - Biochemistry 2022Quote: ... The mixture was incubated on ice for 10 minutes then clarified at 90,000 rpm (TLA-100; Beckman Coulter), 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... cleared supernatants were loaded on a linear 10%–40% sucrose gradient and ultracentrifuged in a SW41Ti rotor (Beckman) for 1 hour and 30 min at 270,000 g at 4°C in a Beckman Optima LE-80K Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... The polymerized GMPCPP-microtubules were pelleted by 90,000 rpm for 10 min at 37°C (TLA120.1 Beckman Coulter) and suspended in warm (37°C ...
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bioRxiv - Genetics 2020Quote: ... The library was then amplified (10 cycles) and size selected using AMPure XP beads (Beckman Coulter, California, US) for fragments approximately 300bp in length ...
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bioRxiv - Genetics 2020Quote: ... Illumina modified adapters added with 10 new PCR cyles after purification with AMPure XP beads (Beckman-Coulter, USA) were ...
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bioRxiv - Cell Biology 2024Quote: ... Linear sucrose gradients of 10–50% were prepared in SW41 centrifugation tubes (Beckman Coulter, Ultra-Clear™ 344059), using the Gradient Station (BioComp) ...
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bioRxiv - Cancer Biology 2024Quote: ... whereas blood derived invitro expanded NK cells were pre-treated with anti-NKG2A (10 μg/ml) (Beckman Coulter) antibody for 1 hour ...
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bioRxiv - Systems Biology 2023Quote: ... Tubes were spun at 31,000 rpm for 3h at 10°C in an SW41 rotor (Beckman Coulter ultracentrifuge) and the resulting phage band had a buoyant density of 1.36 g/ml ...
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bioRxiv - Microbiology 2023Quote: ... The samples were finally centrifuged (5250 g, 10 min) in an Allegra X-15R centrifuge (Beckman Coulter, USA) using 96-well-filter plates (AcroPrep Advance 96-well 0.2 μm GHP 636 membrane filter plate ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Biophysics 2023Quote: ... transferred to 50 ml polypropylene tubes and centrifuged for 10 minutes at 22°C at 3000 xg (Beckman Coulter Allegra X-12R ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were harvested by two rounds of centrifugation (6,000 rpm, 10 min, 4°C, JLA-9.1000 rotor; Beckman), resuspended in Buffer A containing 50 mM Tris-HCl pH8.0 ...
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bioRxiv - Microbiology 2024Quote: ... 10 ml of viral stock was concentrated in 12 ml polypropylene tubes (14 x 89 mm, Beckman Coulter) by ultracentrifugation (Optima XPN-80 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected by centrifugation (8,000 rpm, 10 minutes at 4°C, JLA-10.500 rotor (Beckman Coulter)) ...
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bioRxiv - Cell Biology 2024Quote: ... 10 and 14 to be analyzed by flow cytometry on CytoFLEX V5-B5-R3 Flow Cytometer (Beckman Coulter). After a saturation step with Gamma Immune (Sigma Aldrich ...
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bioRxiv - Genomics 2021Quote: ... Libraries were amplified for a total of 8-10 cycles and DNA purified using AMPure XP beads (Beckman Coulter). The quality and concentration of ATAC-seq libraries were evaluated using the Bioanalyzer DNA High Sensitivity Assay (Agilent Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... Lentivirus containing > 10 kb length insert (inducible CRISPRi and CRISPRa) was concentrated using AVANTI J-30I centrifuge (Beckman Coulter) with JS-24.38 swing rotor at 72000g for two hours at 4 degree ...
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bioRxiv - Biochemistry 2020Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Cell Biology 2021Quote: ... with or without 5µM Gli for 15 minutes at room temperature in 1x BRB80 supplemented with 0.1 mg/mL BSA and then subjected to sedimentation by ultracentrifugation at 50,000 rpm for 10 minutes at 27°C in a TLA 120.1 rotor (Beckman Coulter). Pellets were subsequently resuspended and the amount of protein in each pellet and supernatant was analyzed by SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were collected by centrifugation (8 000 rpm for 10 min at 4 °C, JLA-16.25 Beckman Coulter rotor), pellets frozen in liquid nitrogen and stored at −80°C ...
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bioRxiv - Microbiology 2020Quote: ... and separated in potassium tartrate-glycerol gradient 10–35% for 2 hrs at 175,000 ×g (Beckman SW41 Ti rotor). Purified viral particles were suspended in 10 µg/ml BSA solution or 10 µg/ml CbAE-1 solution and incubated for 1 hr at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and 500 μl of a 10 ml resuspension of cells from each flask diluted with 19.5 ml Isoton II Diluent (Beckman). PD time ...
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bioRxiv - Microbiology 2020Quote: ... Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman), and supernatant transferred to a fresh tube.
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was then clarified 200 x g for 10 min at 4°C in an Allegra centrifuge (Beckman). Supernatant was transferred to a fresh tube and further clarified by centrifugation at 18,000 x g for 10 min at 4°C in a microfuge (Beckman) ...
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bioRxiv - Genetics 2022Quote: ... The lysate was centrifuged at 2000 rpm for 10 min at 4 °C in a JA-20 rotor (Beckman). The supernatant from the previous step was centrifuged in a Beckman Coulter high speed centrifuge equipped with a JA-20 rotor at 6000 rpm for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... equivalent amounts of ribosomes for each lysate was loaded onto each 10%-45% sucrose density gradient (BioComp Gradient Master machine) for ultracentrifugation at 33,000 rpm for 5 hours (Beckman Optima L80K Ultracentrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was mixed with GMPCPP-seeds from the prior step and then incubated at 37°C for 30 mins followed by centrifugation at 90,000 rpm for 10 min at 37°C (TLA120.1 Beckman Coulter). The microtubule pellets were rinsed twice with 100 μ! warm (37°C ...
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bioRxiv - Microbiology 2022Quote: ... phage suspension was layered on top of a 5–20 % sucrose (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) and centrifuged (Beckman Optima L-K90 ...
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bioRxiv - Microbiology 2022Quote: ... The light-scattering zone was collected and further purified in 20– 70 % sucrose gradient (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) by centrifugation (Beckman Optima L-K90 ...
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bioRxiv - Physiology 2022Quote: ... The dissected tissues were held in a glass-bottom dish (35 × 10 mm, IWAKI #3910-035) with an insect pin (φ0.10 mm, Ento Sphinx Insect Pins) and silicone grease (Beckman), and 20 μL of SDM was added to cover the tissue ...
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bioRxiv - Biochemistry 2022Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Systems Biology 2020Quote: ... the 500xg supernatant from enzymatic digestion of cardiovascular tissues underwent further ultracentrifugation at 10,000xg for 10 minutes at 4°C (Beckman Coulter ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 mM NH4Cl) and centrifuged for 16 h at 4°C with 18,000 rpm in an SW60Ti rotor (Beckman). Gradients were hand-fractionated and ribosome concentrations were determined by absorbance at 260 nm (A260 ...
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bioRxiv - Immunology 2021Quote: ... overlaid onto 5 ml of 10% sucrose in NTE (135 mM NaCl, 10mM TrisCl, ph 7.50, 1mM EDTA) in ultra-centrifuge tubes (Beckman) and centrifuged at 25,000 rpm for 90 minutes at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was loaded onto a 10-55% sucrose gradient followed by ultracentrifugation (Beckman Coulter Optima XPN-100 ultracentrifuge) at 32,500 × rpm at 4 °C for 80 minutes in the SW41 rotor ...
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bioRxiv - Cell Biology 2022Quote: ... milk was prevented from cooling down and was processed within 20 minutes after collection by 2 rounds of centrifugation at 3,000xg for 10 minutes at 23°C (Beckman Coulter Allegra X-12R ...
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bioRxiv - Molecular Biology 2023Quote: ... 72 μL of 10% sarkosyl was added to 650 μL of each sample in ultra-centrifuge tubes (Beckman Coulter) and samples were rotated end-over-end at room temperature for one hour ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Plant Biology 2023Quote: ... were added and incubated for 10 minutes at room temperature and purified with 0.4X AMPure XP beads (Beckman Coulter). Adapter Mix II (ONT ...