Labshake search
Citations for Beckman :
251 - 300 of 1911 citations for 4 Methoxy 2 2 3 4 5 5 Hexacb Unlabeled 50 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Hettich) at 4°C and then centrifuged at 40000 rpm for 4h30 at 4°C using SW41Ti rotor (Beckman). The supernatant was then UV inactivated at a wavelength of 254 nm for 4 min (3 times ...
-
bioRxiv - Microbiology 2023Quote: ... The lysates containing matured PsVs were loaded on an OptiPrep gradient that had been stabilized at least 1 h and centrifuged at 50k x g for 3.5-4 h at 4°C in a SW-55Ti rotor (Beckman). Fractions were collected in siliconized tubes and subjected to SDS-PAGE followed by Coomassie blue staining to assess the abundance of L1 and L2 proteins ...
-
bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
-
bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
-
bioRxiv - Plant Biology 2020Quote: ... Double size selection was carried out by adding 50 μL of a 1:4 (0.5X) mixture of AMPure XP Beads (Beckman Coulter, Brea, USA) to PEG buffer (20% PEG w/v ...
-
bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
-
bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
-
bioRxiv - Cell Biology 2022Quote: ... and the pellet was resuspended with 5 ml buffer B and transferred into a 14 ml ultracentrifuge tube (331372, Beckman Coulter). 9 ml Buffer C (1.8 M sucrose ...
-
bioRxiv - Physiology 2019Quote: ... Lysates (≈11 ml) were centrifuged at 26,000xg for 30 min at 4°C in a SW41Ti rotor (Beckman). The floating lipid droplet layers were harvested with a glass pipette and adjusted to 25% sucrose and 100 mM sodium carbonate ...
-
bioRxiv - Biophysics 2019Quote: ... Total volume was centrifuged at 10000 xg for 30 minutes at 4°C at 10000 xg for 30 minutes at 4 °C (Beckman Coulter Optima L-80 XP Ultracentrifuge ...
-
bioRxiv - Biochemistry 2020Quote: ... which were centrifuged at 50,000rpm for 4 hours at 4°C using a TLS55 rotor and an Optima MAX-XP Ultracentrifuge (Beckman Coulter). The sample was physically fractionated into 10 fractions of 85 uL each ...
-
bioRxiv - Molecular Biology 2022Quote: ... 800 µl of EDTA-treated lysate was layered on top of the sucrose gradient and ultracentrifuged at 35000 rpm for 4 hours at 4 °C in a SW41Ti rotor (Beckman). After centrifugation ...
-
bioRxiv - Neuroscience 2023Quote: ... the samples were filtered with a membrane pore size of 0.2 μm and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
-
bioRxiv - Plant Biology 2021Quote: ... sucrose was transferred to the bottom of the tube using a Pasteur pipette and centrifuged at 27,000 rpm for 3 hours at 4° C using an SW28 Ti rotor (Beckman Coulter, Brea, CA). Each pellet containing the virus particles was resuspended in 1 mL resuspension buffer overnight with gentle shaking at 4 °C ...
-
bioRxiv - Microbiology 2019Quote: ... The filtrate was then re-centrifuged at 125000 × g at 4° C for 3 hours (Optima™ L-XP ultracentrifuge, Beckman, USA). The obtained pellet was suspended in 300 µl sterile phosphate-buffered saline (PBS) ...
-
bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 16000 ×g for 3 hours at 4 °C in an Avanti J-25 centrifuge (Beckman Coulter Life Sciences, USA) with a JA-25.50 fixed-angle aluminum rotor (Beckman Coulter Life Sciences ...
-
bioRxiv - Molecular Biology 2021Quote: ... sucrose gradients prepared with lysis buffer without Triton X100 and centrifuged at 32,000 rpm for 3 hrs at 4 °C in an SW41Ti rotor (Beckman Coulter, Cat:331362). The sucrose gradients were fractionated using BR-188 Density Gradient Fractionation System with 60% (m/v ...
-
bioRxiv - Cell Biology 2020Quote: ... 4°C in a TLA 100.3 rotor (Beckman). P13 ...
-
bioRxiv - Cell Biology 2022Quote: ... 4°C (Type 45 Ti rotor, Beckman Coulter) for 30 min to collect supernatant ...
-
bioRxiv - Biochemistry 2020Quote: ... 4 °C (Beckman Coulter Optima LE-80K Ultracentrifuge). The clarified lysate was placed in a beaker on a stir plate at 4 °C and concentrated ammonium sulfate (5 mM HEPES pH 7.5 ...
-
bioRxiv - Neuroscience 2019Quote: ... 4°C (Optima™ MAX Ultracentrifuge, Beckman Coulter) to sediment amorphous aggregates ...
-
bioRxiv - Neuroscience 2020Quote: ... for 4 h with SW28 rotor (Beckman Coulter). The concentrated vector was stored at −80 °C until use ...
-
bioRxiv - Biochemistry 2022Quote: ... 4 °C on a Sorvall WX90 ultracentrifuge (Beckman) with SW55Ti rotor ...
-
bioRxiv - Molecular Biology 2022Quote: ... 4°C in SW-41Ti rotor (Beckman Coulter). Subsequently ...
-
bioRxiv - Plant Biology 2021Quote: ... 4 °C for 18 h (Beckman SW41 rotor). The sugar solution contains 0.65 M sucrose ...
-
bioRxiv - Biochemistry 2022Quote: ... 4°C using a SW41Ti rotor (Beckman Coulter). A BioComp fractionator was used to collect fractions 1 – 16 ...
-
bioRxiv - Neuroscience 2022Quote: ... at 4°C in a Beckman ultracentrifuge (Beckman). After centrifugation ...
-
bioRxiv - Systems Biology 2022Quote: A particle size analyzer (Multisizer 4, Beckman Coulter) was used to count the number of cells ...
-
bioRxiv - Neuroscience 2023Quote: ... but 2.5 mL for 5%) and ultracentrifuged at 100,000 x g (SW 40 Ti rotor, Beckman Coulter) for 16 h using ultra-clear tubes (Beckman Coulter ...
-
bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
-
bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
-
bioRxiv - Microbiology 2021Quote: ... the SBV samples were purified via sucrose density gradient centrifugation (10–50% range) at 32,500 rpm for 4 h with an SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter Inc., Brea, CA). The SBV band that corresponded to the 40% sucrose gradient was harvested and then centrifuged at 25,000 rpm for 12 h ...
-
bioRxiv - Cancer Biology 2022Quote: ... the sample was centrifuged at 268,000 ×g at 4°C for 38 min with an MLA-50 rotor (Beckman Coulter, Brea, CA, USA). The top layer and subsequent 10 fractions (fractions 1–10 ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 ml of 50% and 3 ml of 40% sucrose) in Ultra-Clear Beckman tubes and centrifuged at 26000 rpm (SW32Ti rotor, Beckman Coulter) for 1 h at 4°C ...
-
bioRxiv - Immunology 2021Quote: ... were incubated with monoclonal antibodies at 4°C for 30 min and then fixed with 4% paraformaldehyde before analysis on a Cytoflex cytometer (Beckman Coulter). Data were analyzed by Flow Jo software v.10.7 (BD Biosciences) ...
-
bioRxiv - Cell Biology 2021Quote: ... and loaded onto of a step-wise sucrose gradient as described by the manufacturer and subjected to ultracentrifugation at 150,000 xg for 4 h at 4 °C using SW50.1 rotor (Beckman Coulter, Mississauga, ON). Fractions were then collected and subject to denaturation with Laemmli buffer until further use.
-
bioRxiv - Cell Biology 2022Quote: ... Equal volume (2 ml i.e 1600 µg) of cell extracts were loaded in ultracentrifuge tubes (Beckman). Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman) ...
-
bioRxiv - Microbiology 2020Quote: ... of 1.5 mL and then centrifuged 45 min at 100,000g at 4°C in a TLA-100 ultracentrifuge (Beckman Coulter). The supernatant contains the soluble fraction and the pellet corresponds to the total membranes ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.5 mM DTT, 100 μg/ml cycloheximide) and centrifuged (160,000 g, 120 min, 4°C) in an SW41Ti rotor (Beckman). After centrifugation the sediment in the tube went through a UV detector at the wavelength of 254nm and simultaneously the required fractions (>=5 ribosomes ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... The filtered supernatant from one 400 ml batch was then pelleted (1.5 hour at 24 000 rpm at 4 °C with Beckman Coulter L-90K centrifuge and 45 Ti rotor ...
-
bioRxiv - Neuroscience 2023Quote: ... The tube was topped up to 23 mL with PBS and centrifuged at 59,300 RPM for 30 min at 4°C using a Ti70 rotor (Beckman). The floating lipid layer and supernatant were discarded and the pellet was transferred to a 2 mL tube in 50 μL PBS per g initial grey matter ...
-
bioRxiv - Molecular Biology 2023Quote: ... Pellets were pooled in 1 ml PBS and centrifuged at 100,000 X g for 1,5 h at 4°C in 1,5 ml tubes in a Ti-45 rotor (Beckman Coulter) using adapters (#11004 ...
-
bioRxiv - Molecular Biology 2022Quote: Procyclic form cell lines for depletion of either TbPH1 or TbKifX2A were induced at a density of 2×106 cells/ml with 1 µg/ml doxycycline and growth monitored daily with a Z2 Coulter Counter Analyzer (Beckman Coulter). Cells were diluted back to the initial cell density every day ...
-
bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
-
bioRxiv - Biochemistry 2024Quote: ... 50 mM Tris-HCl pH 7.4) and ultracentrifuged at 250 000 x g for 2 h (Beckman Optima TL 100 Ultracentrifuge). After ultracentrifugation ...
-
bioRxiv - Physiology 2021Quote: ... sEV were further isolated from lEV-depleted supernatants following a 100,000 xg ultracentrifugation step for 1 hour at 4°C (rotor MLA-50, Beckman Coulter Optima MAX-XP Ultracentrifuge) and two washes in NaCl before resuspension in NaCl.
-
bioRxiv - Genetics 2021Quote: ... Reactions were then pooled in a 5 ml tube and purified using a 0.7x SPRIselect (Beckman Coulter B23318) cleanup and eluted in 450μL of TE ...
-
bioRxiv - Molecular Biology 2019Quote: ... and centrifuged at 30,000 rpm for 4 h at 4°C using an SW-40 rotor in the Optima L-90K Ultracentrifuge (Beckman Coulter, USA). To identify resultant density gradient fractions containing virus particles ...
-
bioRxiv - Biophysics 2024Quote: ... The gradients were then centrifuged at 187,000 × g for 4 h at 4°C in a SW 41 Ti rotor (Beckman Coulter, Brea, CA). Bands corresponding to purified virus particles (∼1.36 g/mL ...