Labshake search
Citations for Beckman :
201 - 250 of 885 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Microbiology 2023Quote: ... The tube was centrifuged for 3 hours at 150’000 x g (4°C) using a SW 32.1 Ti swinging bucket (369651, Beckman Coulter). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01% Sodium Azide in PBS and measured by recording 3 × 104 events using CytoFLEX and Gallios cytometers (Beckman Coulter). The resulting data were analysed using FlowJo v10 (TreeStar) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 20nM of gemcitabine and cells were counted 3 days later using the Z1 Coulter (Beckman).
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bioRxiv - Bioengineering 2023Quote: ... MB concentration and number- and volume-weighted size distributions were measured using a Multisizer 3 Coulter Counter (Beckman Coulter). MB concentration (𝑐i ...
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bioRxiv - Neuroscience 2023Quote: ... the liposomes were isolated by centrifugation for 3 h 40 mins at 55,000 rpm at 4°C in a SW 60 rotor (SW 60 Ti, Beckman). 20 µl were harvested from the top of the gradient and mixed with 8 µl of 4x Laemmli buffer (final 62.5 mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... and centrifuged for 3 h at 260,000 g using an SW 55 Ti swinging-bucket rotor (Beckman Coulter, USA). The final supernatant was discarded ...
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bioRxiv - Microbiology 2023Quote: ... and RNA were purified using 3 volume of absolute isopropanol and 1.8 volume of Agencourt RNAClean XP beads (Beckman). RNA was eluted with 6 µL of water ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose density gradients (5%–45% w/v) were freshly made in SW41 ultracentrifuge tubes (Beckman) using a Gradient Master (BioComp Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 mM MgCl2) and centrifuged at 45,000 rpm (SW 55 Ti rotor, Beckman Coulter, Inc.) (adapted from Liang et al ...
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bioRxiv - Immunology 2019Quote: ... 5 µl of cDNA per cell was cleaned using Agencourt AMPure XP beads (Beckman Coulter) at a 1.0x ratio on a Hamilton STAR liquid handler (Hamilton Robotics) ...
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bioRxiv - Microbiology 2021Quote: ... Product were PCR amplified by 5 cycles and cleaned up using SPRI beads (Beckman Coulter) and subjected to paired-end 300bp sequencing on Illumina Miseq ...
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bioRxiv - Biochemistry 2023Quote: ... Ultracentrifugation was carried out for 5 hours at 133,000xg on a SW60-Ti (Beckman Coulter) rotor to resolve each gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... After 5-7 days cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... pH 7.4) and loaded into a centrifuge tube (Beckman Cat. No. 344057, 5 ml capacity). One milliliter of 20% sucrose in 20 mM HEPES pH 7.4 ...
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bioRxiv - Genomics 2024Quote: ... After 5 days cells were harvested for genomic DNA purification using Agencourt DNAdvance (Beckman Coulter). Transfection of human primary hepatocytes were performed using Lipofectamine MessengerMAX Transfection Reagent (Thermo Fisher) ...
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bioRxiv - Biophysics 2024Quote: ... the microtubules were centrifuged for 5 min at 22,000 RPM (TLA100.0, Beckman Optima MAX-XP) at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The BKE (ErmR) library was arrayed in 384-well plates using a Biomek FX liquid handling robot (Beckman Coulter) and stored as glycerol stock ...
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bioRxiv - Genomics 2019Quote: ... Preparation of sort plates and all downstream pipetting steps were performed on a Biomek i7 Automated Workstation (Beckman Coulter). After addition of 1 µL 0.2% SDS ...
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bioRxiv - Genomics 2020Quote: ... Preparation of sort plates and all downstream pipetting steps were performed on a Biomek i7 Automated Workstation (Beckman Coulter). After addition of 1 μL 0.2% SDS ...
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bioRxiv - Microbiology 2019Quote: ... Selected hits were dispensed into 384 well Echo plates using the Biomek FX automated liquid handling workstation (Beckman Coulter); two-fold serial dilutions of each compound in DMSO was performed using an Echo 550 liquid handler (Labcyte).
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bioRxiv - Microbiology 2019Quote: ... was added into 20μl cell lysate and luciferase activity was measured using a DTX800 Multimode plate reader (Beckman Coulter). The remainder of the cell lysate was collected for western blots and GAPDH immunostaining ...
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bioRxiv - Genomics 2021Quote: ... Preparation of sort plates and all downstream pipetting steps were performed on a Biomek i7 Automated Workstation (Beckman Coulter). After addition of 1 µL 0.2% SDS ...
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bioRxiv - Immunology 2021Quote: ... Optical density (OD) at 620 nm was determined using a DTX 880 multimode plate reader (Beckman Coulter, Brea, CA). Mouse bone marrow-derived macrophages were cultured in DMEM supplemented with 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2020Quote: ... we evenly pooled all wells of any given plate and cleaned up the library using SPRI beads (Beckman Coulter) using a dual purification of 0.8× beads followed by 0.8× or 0.7× beads ...
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bioRxiv - Cancer Biology 2020Quote: ... Live single CTCs were sorted directly into 96-well GravityTRAP™ ULA Plates using aMoFlo cell sorter (Beckman Coulter). An inverted microscope was used to manually confim that there was 1 CTC per well ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP-positive cells were single-cell sorted into 96-well plates using a MoFlo Astrios Cell Sortor (Beckman Coulter) and cultured for another 2~3 weeks to allow for clonal expansion ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 nL of compounds in DMSO were pinned into the plates using a Biomek FX Automated Workstation (Beckman Coulter). After 72 hours ...
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bioRxiv - Genomics 2021Quote: ... plates of 96 single cell libraries purified using a 1.2x volume of AMPure XP beads (Beckman Coulter, catalog # A63881). All libraries were then quantified using the Qubit dsDNA HS kit (Life Technologies) ...
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bioRxiv - Genomics 2019Quote: ... Preparation of sort plates and all downstream pipetting steps were performed on a Biomek i7 Automated Workstation (Beckman Coulter). After addition of 1 µL 0.2% SDS ...
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bioRxiv - Genetics 2021Quote: ... Preparation of sort plates and all downstream pipetting steps were performed on a Biomek i7 Automated Workstation (Beckman Coulter). After addition of 1 µL 0.2% SDS ...
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bioRxiv - Biophysics 2023Quote: ... the compound library was reformatted into 384 well Echo compatible plates using the Biomek FX (Beckman Coulter, Miami, FL) and 5 nL of either compound (columns 3-22 and 27-46 ...
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bioRxiv - Biochemistry 2024Quote: ... and mid controls dispensed in identical sets across the plate using the Echo 550 dispenser (Beckman Coulter, Brea, CA) for compounds/DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... A volume of 0.4 μL of ligand was added to cell plates using a BiomekFX Lab Automation Workstation (Beckman) and a previously calibrated pin tool ...
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bioRxiv - Cell Biology 2024Quote: ... mTurquoisehigh cells were sorted into 96-well plates at 1 cell/well using a MoFlo Cell Sorter (Beckman Coulter). For cells transfected with sgRNA/Puro plasmids ...
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bioRxiv - Molecular Biology 2020Quote: ... Debris was clarified by centrifuging at 18,000 ×g followed by fractionation on a discontinuous sucrose gradient (10-50 %) by ultracentrifugation at 39,000 rpm for 3 h in an SW41-Ti rotor (Beckman coulter). Monosome fractions were collected by pumping of Fluorinert FC-40 and using a fraction collector by measuring UV trace ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Genomics 2019Quote: ... Samples were size selected using a 0.5X Ampure XP cleanup to remove large fragments followed by 3 consecutive 1.5X Ampure XP (Beckman Coulter # A63880) bead cleanup followed by a final 1.2X Ampure XP cleanup to completely remove all residual primers and resuspended in a final volume of 20 µL EB using the manufacturer’s recommended protocol.
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bioRxiv - Cell Biology 2019Quote: ... Monosomes were isolated by sucrose gradients (10-50%) and centrifuged at 40,000 rpm for 3 hours in a SW41 rotor (Beckman Coulter). The extracted RNA was size-selected from 15% denaturing PAGE gels ...
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bioRxiv - Cancer Biology 2020Quote: The barcode split of the cDNA amplification reaction (from 10x Single Cell 3’ v3 Step 2.2) was purified via 1.2x SPRI Select (Beckman Coulter #B23317). cDNA products were eluted in 40 µL of EB ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants (3 ml) were loaded on the top of step gradients preformed in polyallomer tubes (14 by 89 mm, Beckman), containing 2 ml each of 1.82 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.3 M, 2.5 M) as indicated in the method (Vella et al., 2017) in ultra-clear SW40Ti tubes (Beckman Coulter). The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... was overlaid on 25% sucrose in PBS and centrifuged at 26,000 rpm for 3 h at 4C using SW28 rotor (Beckman Coulter). Pellets were dissolved in PBS overnight at 4C and 1/3 of used for RNA was extraction using Macherey Nagel Virus RNA isolation kit without carrier RNA according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are visualized on Cytoflex S, 3 lasers (488 nm, 405 nm and 638 nm) and 13 detectors (Beckman Coulter). Live cells were gated based on side scatter and forward scatter ...
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bioRxiv - Cell Biology 2021Quote: ... The post-nuclear supernatants were ultracentrifuged using SW41Ti rotor at 100,000 g for 3 h at 4 °C on Optima L-90K (Beckman Coulter). The fraction between 0.4–0.5 M sucrose was collected ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... UV-inactive SARS-CoV-2 was then partially-purified using a 20% sucrose cushion centrifuged at 175,000 x g for 3-4 hr using SW32Ti rotor (Beckman Coulter). The sample that has passed through the sucrose was collected and concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... and the cleared supernatant was loaded on a 3 to 30% iodixanol gradient (OptiPrep) and ultracentrifuged for 5h at 36,000 rpm in an SW60 rotor (Beckman Coulter). Fractions were collected manually through aspiration and RNA or proteins were isolated for further analysis.
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bioRxiv - Microbiology 2020Quote: ... The filtered supernatant was then spun through a 20% sucrose cushion at 48,000 rpm for 3 hours in a SW55Ti rotor (Beckman Coulter). The SVP pellets were resuspended in a 4X non-reducing buffer (Alfa Aesar ...