Labshake search
Citations for Beckman :
201 - 250 of 259 citations for L lactate dehydrogenase B chain LDH B Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... 50 μL of the amplified ATAC-seq library was subjected to double-sided size selection using magnetic beads (AMPure XP, Beckman Coulter). DNA bound to the beads was washed twice with 80% ethanol and then eluted in 20 μL H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting complexes were either used for structural analysis or resolved on a 15 mL 10-40% sucrose density gradient centrifuged at 35000 rpm for 135 minutes using SW41 rotor on an Optima L-90K ultracentrifuge (Beckman Coulter), and the resolved particles were fractionated as previously described (59) ...
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bioRxiv - Physiology 2023Quote: ... was ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 × g for 70 min at 4°C (Optima L-100 XP Ultracentrifuge, 90 Ti Fixed-Angle Titanium Rotor; Beckman Coulter). The final EV pellet was suspended in 50 µl of PBS without Ca2+ and Mg2+ (Lonza ...
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Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in SynechocystisbioRxiv - Synthetic Biology 2023Quote: ... Ultracentrifugation was performed in a SW40 Ti swinging-bucket rotor at 130000 × g for 16 h at 20 °C (Optima L-80 XP, Beckman Coulter).
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bioRxiv - Bioengineering 2023Quote: ... Samples were ultracentrifuged at 100,000×g for 70 min at 4°C using the Beckman OptimaTM L-80 XP ultracentrifuge (Beckman Coulter, CA). The supernatant was discarded ...
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... The pre-concentrated culture was centrifuged (10000 g, 10 min) in 1-L buckets (Avanti J-265 XP, Beckman Coulter, USA). The biomass reached a concentration of approximately 40 % (dry weight - DW ...
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bioRxiv - Microbiology 2021Quote: ... the percentages of anti-human IgG postive cells in GFP positive cells were then analyzed using flow cytometer (Cytoflex S, Beckman). LIVE/DEAD fixable aqua (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... PCR products with no hashtag libraries (spleen, mouse lung, and human lung cells) were combined and purified twice with 0.65× AmPure XP beads (Beckman Coulter) and eluted with 21 μL of nuclease-free water ...
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bioRxiv - Cancer Biology 2020Quote: ... The virally-transduced human CD34+ were sorted for GFP reporter expresssion on a MoFlo high-speed cell sorter (Beckman-Coulter) located at the New York Blood Center ...
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bioRxiv - Immunology 2021Quote: ... as assessed by flow cytometry with Korman orange-conjugated mouse anti-human CD19 monoclonal antibody (mAb) (Beckman Coulter, CA, USA). The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza ...
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bioRxiv - Immunology 2023Quote: Human TCR Vβ repertoire analysis was performed using antibodies against the most frequent TCR Vβ variants (covering about 70% normal human TCR Vβ repertoire, as reported by Beckman Coulter “Beta Mark TCR Vbeta Repertoire Kit” (cat.nr. ...
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bioRxiv - Immunology 2024Quote: Flow cytometry mAbs included directly-labeled mouse anti-human CD4-PE-Cy5 (clone 13B8.2) and TCR-αβ–PE-Cy5 (clone IP26A) from Beckman Coulter ...
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bioRxiv - Molecular Biology 2019Quote: ... and centrifuged at 30,000 rpm for 4 h at 4°C using an SW-40 rotor in the Optima L-90K Ultracentrifuge (Beckman Coulter, USA). To identify resultant density gradient fractions containing virus particles ...
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bioRxiv - Molecular Biology 2019Quote: ... was clarified by centrifugation at 10,000 rpm for 30 min at 4 °C using an SW-28 rotor in an Optima L-90K Ultracentrifuge (Beckman Coulter, USA) and was then ultracentrifuged at 25,000 rpm for 6 h at 4 °C using the same rotor ...
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bioRxiv - Cancer Biology 2020Quote: ... CM was then sequentially ultra-centrifuged at 100,000 x g at 4 ° C for 2 hrs in a Beckman Coulter Optima L-100 XP Ultracentrifuge using an SW41-Ti rotor and polycarbonate centrifugation tubes (Beckman Coulter; #331372). The EV pellet ...
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bioRxiv - Biophysics 2021Quote: ... then the supernatant was ultracentrifuged at 100000×g for 20 min at 4 °C using an Optima L-100K ultracentrifuge (Beckman Coulter, USA) equipped with a SW 40 Ti swinging bucket rotor ...
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bioRxiv - Bioengineering 2021Quote: ... the culture medium was replaced with a similar volume of DMEM supplemented with 0.5% exosome-free FBS [the supernatants were centrifuged at 100,000 × g for 12 h (Beckman Coulter, Optima L-100XP) using normal FBS] ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was then centrifuged at 4° C in the SW32Ti rotor with a speed of 30,000 rpm for 1 h to pellet the membrane fraction using an Optima L-90K Ultracentrifuge (Beckman Instruments, Brea, CA). The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace ...
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bioRxiv - Microbiology 2019Quote: ... The filtrate was then re-centrifuged at 125000 × g at 4° C for 3 hours (Optima™ L-XP ultracentrifuge, Beckman, USA). The obtained pellet was suspended in 300 µl sterile phosphate-buffered saline (PBS) ...
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bioRxiv - Biochemistry 2022Quote: ... 000 g at 4°C for 30 min in a Beckman Coulter Optima L- 100 XP ultracentrifuge using 14 mm × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Ultra-pure mitochondria were harvested at the interphase between 19% and 40% and washed three times with 1xM buffer ...
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bioRxiv - Biophysics 2020Quote: ... cell lysate was centrifuged by using The Beckman Optima™ L-80 XP Ultracentrifuge at 40000 rpm for 30 minutes at 4°C by using Ti45 rotor (Beckman, USA). After ultracentrifugation the pellet which contains membranes and insoluble debris was discarded and clear supernatant applied to nickel affinity chromatography by using a Ni-NTA agarose resin (QIAGEN ...
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bioRxiv - Immunology 2021Quote: ... were loaded onto 5-25% (w/v) linear sucrose gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 3 h 15°C ...
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bioRxiv - Immunology 2021Quote: ... was loaded onto the top of 6–8 M GuHCl gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 2.75 h 15°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour with Beckman Optima™ L-80XP Ultracentrifuge equipped with Ti45 rotor (Beckman, USA). The pellet containing membranes and cell debris was discarded ...
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Isolation and characterization of a novel phage of Vibrio parahaemolyticus with biocontrol potentialbioRxiv - Microbiology 2022Quote: ... for purification and centrifuged at 200,000 × g at 4°C for 24 h using an Optima L-100 XP ultracentrifuge (Beckman Coulter, CA, USA). The purified phage particles were dialyzed through 30-kDa superfilters (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were centrifuged at 250000 g for 60 minutes in a swinging bucket rotor of the ultracentrifuge (Beckman Coulter Optima L-60). Then ...
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bioRxiv - Physiology 2023Quote: ... via centrifugation in a SW41 rotor at 42,000 g at 4 °C for 1 hour in a Beckman Coulter Optima L-100 XP ultracentrifuge using 14 × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Mitochondria were harvested at the interphase between 19 and 40% Percoll ...
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bioRxiv - Genetics 2024Quote: We separate the resulting product into long (“L”) and short (“S”) molecular libraries from the same PCR reactions by using SPRIselect beads (Beckman Coulter, #B23319). The next step involves further preparing libraries for long-read sequencing ...
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bioRxiv - Biochemistry 2024Quote: 3 L of over-expressed cells were harvested immediately by centrifugation (Beckman Coulter Avanti J-20 XPI centrifuge, Beckman JLA-8.100 rotor) at 6,000 x g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The centrifugation is performed on a SW60Ti rotor at 40 000 g for 22 minutes at 4°C (Beckman Optima L-90K Ultracentrifuge). The mitochondrial fraction (400µL ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was centrifuged again at 26000 × g at 4 °C for 15 min using an Optima L-90K (Beckman Coulter, Brea, CA) ultracentrifuge with a 70-Ti fixed angle rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... previously centrifuged for 16 h at 120,000 g to remove bovine exosomes using 40.1Ti rotor (L-80XP; Beckman Coulter®, Brea, CA, USA).) ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... Ten milliliters of allantoic or amniotic fluid was centrifuged at maximum speed (16,000 × g) for 30 min at 4 °C using a floor ultra centrifuge (Beckman Optima L-60 Ultracentrifuge). After removal of the supernatant ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Results in LIFE PERSUADED project were reported un-adjust (μg/L) and adjusted (μg/g crea) to creatinine concentrations measured using the Jaffe method (Beckman Coulter, Brea, CA) [95 ...
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bioRxiv - Plant Biology 2023Quote: ... 700 ng genomic DNA in 55 μL ddH2O was sheared to ~550 bp using Covaris E220 (Woburn, MA) and size-selected using AMPure XP beads (Beckman Coulter, Indianapolis, IN). The fragmented genomic DNA was hybridized with xGen Lockdown probes (Table S2 ...
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bioRxiv - Plant Biology 2023Quote: ... The disrupted cells were subjected to ultracentrifugation at 4°C and 235000 g for 1h (Beckman Optima™ L-80 XP, rotor Ti45). The resulting supernatant was loaded onto an Äkta purifier system (GE HealthCare ...
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bioRxiv - Bioengineering 2024Quote: PCR amplicons of the CAR and mCherry transgenes were generated as described previously.34 PCR products were pooled into 600 μL and were purified using a singular round of Solid Phase Reversible Immobilization (SPRI) cleanup using AMPure XP beads (Beckman Coulter, Brea, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... a 500 µL sample of suspension culture was measured using trypan blue staining with the Vi-CELL™ XR cell viability analyzer (Beckman Coulter, USA).
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bioRxiv - Cell Biology 2019Quote: Viability of unlabeled and ferumoxytol-labeled murine MSCs (n=4) and human MSCs (n=3) was assessed by Trypan blue dye exclusion using ViCell (Beckman Coulter).
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bioRxiv - Cancer Biology 2019Quote: Cells were stained for 30 min (minutes) with the anti-human monoclonal antibody CD11b-PE (IM2581U, Beckman Coulter, Brea, CA, USA), CD14-FITC (555397 ...
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bioRxiv - Cancer Biology 2024Quote: Analysis of GALNT3 or fibronectin expression on human or mouse pancreatic cancer cells was performed using a flow cytometer (Gallios, Beckman Coulter) with appropriate antibodies ...
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bioRxiv - Immunology 2024Quote: ... The sections were washed and incubated for two hours with anti-human mouse CD14 Texas Red (clone: RMO52) (Beckman Coulter, USA). Afterward ...
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bioRxiv - Microbiology 2019Quote: ... RNA was density separated by centrifugation at 124 000 g at 20 °C for 65 h using an Optima L-90 XP ultracentrifuge (Beckman Coulter, Brea, California, USA). 13 fractions were obtained from each sample ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then spun at 31000 x g (18468 rpm) for 5 min at 4°C in a fixed-angle rotor (50.2 Ti rotor in Beckman Coulter Optima L-100 XP) with slow brake ...
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bioRxiv - Neuroscience 2023Quote: ... A 500 µL aliquot of cell suspension was used to determine cell number and viability using an automated cell counter (ViCell, Beckman Coulter, Brea, CA, USA). Remaining cells were collected by gentle centrifugation (193 x g ...
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bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1).
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bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1) ...