Labshake search
Citations for Beckman :
201 - 250 of 959 citations for 7 16 DIHYDROBENZO A BENZO 5 6 QUINO 3 2 I ACRIDINE 9 18 DIONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... Ultra-centrifugation was performed (160,000 x g; 16 hours; 4°C; SW 32 Ti rotor; Optima XPN-80, Beckman Coulter) and 1mL fractions were collected and analyzed by BN-PAGE ...
-
bioRxiv - Cell Biology 2019Quote: ... the extracted proteins were dialyzed into NaLow and then were loaded onto a 5-20% sucrose gradient made in NaLow buffer and centrifuged at 35,000 rpm for 16 h in a SW41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 ml fractions ...
-
Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Ribosomes were pelleted by centrifugation at 63,000g for 16–19 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). For all ultracentrifugation steps indicated here ...
-
bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
-
bioRxiv - Biophysics 2023Quote: ... The isolated crude membrane extract was loaded on the continuous 20-70 % sucrose density gradient in 20 mM NaH2PO4/Na2HPO4 pH 7.4 and 100 mM NaCl prepared by the Gradient Station (Biocomp) and centrifuged for 16 h at 30.000 rpm (rotor SW 40 Ti, Beckman Coulter). The gradients were collected with the Gradient Station ...
-
bioRxiv - Microbiology 2024Quote: ... The resulting gradient was then subjected to centrifugation for 16 hours at 150,000 x g and 4 °C (SW55 Ti rotor, Beckman-Coulter). Sample banding was visually observed at the interface of the 30% and 20% fractions (Supporting Information ...
-
bioRxiv - Microbiology 2023Quote: ... sucrose gradients prepared with buffer C and centrifuged for 16 h at 71,935 g at 4 °C in a SW28 rotor (Beckman Coulter). Gradients were fractionated on a Biocomp Piston Gradient Fractionator (BioComp Instruments ...
-
bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged (45 min, 16 000 rpm, 4°C, JLA 16.250 rotor, Beckman-Coulter Centrifuge Avanti J25-01) and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
-
bioRxiv - Biochemistry 2024Quote: ... The protein samples were carefully applied to the gradient and centrifuged for 16 h at 130,000 g at 4 °C (Optima XPN-80 Ultracentrifugation from Beckman Coulter). 200 µl fractions were taken from the top of the gradient and the glutaraldehyde quenched with 20 mM Tris pH 7.5 ...
-
bioRxiv - Cancer Biology 2024Quote: ... Each ATAC library was amplified with Nextera primers for 16 PCR cycles and purified with Agencourt AMPure XP (Beckman Coulter) to remove excess primers ...
-
bioRxiv - Immunology 2020Quote: ... KIR2DL2/3/S2 (GL183, PE/Cy5.5, Beckman Coulter,), NKG2C (134591 ...
-
bioRxiv - Systems Biology 2023Quote: ... enumerated using a Multisizer 3 Coulter Counter (Beckman,), and then seeded at the desired density in 6-well tissue culture plates (TPP ...
-
bioRxiv - Genetics 2022Quote: ... 5 mL (Beckman Coulter, B23317) using a DynaMag™-2 Magnet magnetic stand (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2019Quote: ... Sucrose gradients (7-47%) were centrifuged at 39,000 rpm with a SW41-Ti Rotor (Beckman Coulter, Fullerton, CA) for 90 min at 4 °C ...
-
Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: Sedimentation velocity AUC experiments were performed in a ProteomeLab XL-I analytical ultracentrifuge (Beckman Coulter) following standard procedures122–124 ...
-
bioRxiv - Cell Biology 2023Quote: ... and the cells were counted using an i-cell XR cell viability analyzer (Beckman Coulter).
-
bioRxiv - Biochemistry 2024Quote: Sedimentation velocity experiments were carried out using an Optima XL-I analytical ultracentrifuge (Beckman-Coulter) equipped with UV-visible absorbance optics at 20 °C in an AnTi50 rotor and 12-mm double sector Epon-charcoal centerpieces ...
-
bioRxiv - Molecular Biology 2019Quote: ... The cleared supernatant was layered over 15-40% sucrose gradients in disassociation buffer and spun 28000 rpm for 16 hours at 4°C in a SW-32 rotor (Beckman-Coulter). Gradients were fractionated with an ISCO fractionation system and 30S + 50S peaks were combined ...
-
bioRxiv - Systems Biology 2020Quote: ... The resultant supernatant was collected and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55) in 10.4 ml 16×76 mm polycarbonate capped centrifuge tubes (Beckman Coulter 355603), the pellet was washed once in PBS−/− and centrifuged again at 100,000xg for 40 minutes at 4°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
-
bioRxiv - Biochemistry 2019Quote: ... Gradients were centrifuged at 49,123 x g for 16 hours at 4°C using an SW32Ti rotor (Beckman Coulter, ref. 369694). Gradients were fractionated with a Brandel gradient fractionation system using a 1 mm UV cell detector ...
-
bioRxiv - Plant Biology 2022Quote: ... extracts were loaded carefully into thick-walled polycarbonate tubes with three-piece caps (10.4 mL, Polycarbonate Bottle with Cap Assembly, 16 × 76 mm - 6Pk, 355603, Beckman Coulter, USA) pre-filled with 2.5 mL sucrose cushion (SC ...
-
Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in SynechocystisbioRxiv - Synthetic Biology 2023Quote: ... Ultracentrifugation was performed in a SW40 Ti swinging-bucket rotor at 130000 × g for 16 h at 20 °C (Optima L-80 XP, Beckman Coulter).
-
bioRxiv - Biochemistry 2023Quote: ... The sample was run on the gradient for 16 hrs at 4 °C using an Optima XE-90 ultracentrifuge (SW40Ti rotor, Beckman-Coulter) at 30,000 rpm ...
-
bioRxiv - Cancer Biology 2023Quote: ... Crosslinks were reversed with 25 µg Proteinase K for 16 hours at 65°C and DNA was purified using AmpureXP beads (Beckman Coulter). Enrichment was confirmed using qRT-PCR with known positive and negative binding sites for each protein target ...
-
bioRxiv - Biophysics 2023Quote: ... The bottom-loaded 45-10% gradient was then centrifuged at 120 000 x gav for 16 h in a SW40 Ti Swinging Bucket rotor (Beckman Coulter). After ultracentrifugation ...
-
bioRxiv - Molecular Biology 2024Quote: ... the fixed samples were subjected to sucrose cushion centrifugation (7,000x g for 20 min at 16 °C) using a swinging bucket rotor (Beckman, JS-7.5). Coverslips were recovered ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were then spun at 100,000 xg for 16 h at 4°C using a TLS-55 swinging-bucket rotor and benchtop ultracentrifuge (Optima Max-XP, Beckman Coulter). Fractions were collected and loaded in SDS-PAGE for WB.
-
bioRxiv - Synthetic Biology 2024Quote: ... 6 mM MgCl2) with 0.7 M sucrose in Ti-45 tubes (Beckman-Coulter). Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg ...
-
bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
-
bioRxiv - Cell Biology 2020Quote: ... Unbound protein was separated from liposomes by transferring 100μl of this mixture to 7×20mm PC ultracentrifuge tubes (Beckman) and carefully layering an equal volume of 0.75M sucrose buffer followed by 25μl of sucrose-free HK buffer ...
-
bioRxiv - Cell Biology 2019Quote: ... Coverslips were mounted on bottomless 35 mm dishes with a 7 mm microwell using silicone vacuum grease (Beckman Coulter). Conditioned media from the coverslip’s well ...
-
bioRxiv - Neuroscience 2023Quote: ... Fractions were washed by diluting with 7 mL of PBS and ultracentrifuged at 100,000 x g (Type 70.1 Ti rotor, Beckman Coulter) for 2 h ...
-
bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
-
bioRxiv - Biophysics 2021Quote: Sedimentation velocity experiments were conducted in a ProteomeLab XL-I analytical ultracentrifuge (Beckman Coulter, Indianapolis, IN) following standard protocols unless mentioned otherwise (37,38) ...
-
bioRxiv - Biophysics 2021Quote: SV experiments were carried out in a ProteomeLab XL-I analytical ultracentrifuge (Beckman Coulter, Indianapolis, IN) by following standard protocols (Schuck et al. ...
-
bioRxiv - Biophysics 2021Quote: Sedimentation velocity experiments were conducted in a ProteomeLab XL-I analytical ultracentrifuge (Beckman Coulter, Indianapolis, IN) following standard protocols unless mentioned otherwise ...
-
bioRxiv - Biophysics 2022Quote: Sedimentation velocity-AUC measurements were performed with ProteomeLab XL-I (Beckman Coulter Inc., Brea, CA, USA). The optical path and the volume of the cell were 12 mm and 400 μL ...
-
bioRxiv - Molecular Biology 2023Quote: ... Sedimentation velocity experiments were run using Beckman Optima XL-I analytical ultracentrifuge (Beckman Coulter, Fullerton, CA), An60-Ti rotor ...
-
bioRxiv - Cell Biology 2021Quote: ... Virus was concentrated from cell-free culture supernatant by high-speed ultracentrifugation through a 30% sucrose cushion at 80,000xg for 16 hrs in a SW32 rotor (k-factor 321) (Beckman Coulter, Brea, CA).
-
bioRxiv - Biophysics 2022Quote: ... A 5 to 30% linear sucrose gradient was formed above the homogenate and centrifuged at 40,100 rpm and 4° C for 16 hours in a SW55 rotor using an OptimaTM LE-80K Ultracentrifuge (Beckman Coulter, Pasadena, CA). Fourteen 360 μl fractions were collected from the top and analyzed by SDS–PAGE/Western blot with an equal loading volume ...
-
bioRxiv - Biochemistry 2020Quote: ... which was then centrifuged at 160,000×g at 4°C for 16 h in a Beckman MLS–50 rotor (Beckman–Coulter, CA, USA), and 24 fractions were collected ...
-
bioRxiv - Cancer Biology 2022Quote: ... was removed using right-side size selection with AMPure XP beads at a 1:1 and 7:1 bead:sample ratio (Beckman Coulter) described previously26 ...
-
bioRxiv - Genomics 2020Quote: ... PCR was performed (7 cycles) with standard Illumina paired-end primers and the amplicons were then purified with AMPure XP beads (Beckman). The length ...
-
bioRxiv - Immunology 2023Quote: Flow cytometry analysis was performed at the end of the 7 day T cell progenitor differentiation stage using a CytoFLEX LX cytometer (Beckman), and data were compensated using single-stained controls in CytExpert v2.3 ...
-
bioRxiv - Cell Biology 2020Quote: ... amplified by PCR for 6 cycles and purified with AMPure XP beads (Beckman Coulter). The final libraries were pooled and sequenced using the onboard cluster method ...
-
bioRxiv - Genomics 2020Quote: ... (6) the library fragments were purified with AMPure XP Beads (Beckman Coulter, Shanghai, China) to select the insert fragments of 150-200 bp in length ...
-
bioRxiv - Biophysics 2022Quote: ... The 6 kb long 64x repeat was then purified using SPRIselect beads (Beckman Coulter) at a 1 to 0.4 v/v ratio ...
-
bioRxiv - Genomics 2021Quote: ... products were assessed on 6% TBE-gel and purified using 1.0X AMPure (Beckman Coulter) and added to the second PCR reaction that appended dual sample indexes and flow cell adapters ...
-
bioRxiv - Genomics 2021Quote: ... products were assessed on 6% TBE-gel and purified using 0.6X AMPure (Beckman Coulter) and added to the second PCR reaction that appended PacBio sample indexes ...