Labshake search
Citations for Beckman :
201 - 250 of 2117 citations for 2 5 Sulfanyl 4H 1 2 4 triazol 3 yl phenol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2022Quote: ... The membrane was solubilized for 3 hours at 4°C before separation by ultracentrifugation at 100,000 g (Ti45, Beckman) for 45 min ...
-
bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
-
bioRxiv - Microbiology 2024Quote: ... the supernatants were loaded onto 1 mL of 30% sucrose in PBS buffer and centrifuged at 25,000 rpm at 4°C for 3 hours using the 90AT rotor (Beckman). The pelleted viral particles were resuspended in 100 μL of PBS and fixed with formaldehyde (working concentration 0.1% ...
-
bioRxiv - Neuroscience 2024Quote: ... Tubes were centrifugated at 100,000 x g (32,500 RPM) for 3 hours at 4°C using a SW55Ti rotor (Beckman Coulter). Synaptosomes enriched in the interface between 1.0-1.25 M sucrose were collected and diluted with ice-cold 0.1 mM CaCl2 ...
-
bioRxiv - Cell Biology 2024Quote: ... The 100,000xg SEV-containing pellets were resuspended in 3 ml sterile cold PBS and repelleted at 100,000xg for 4 hrs in a TLA110 rotor (Beckman). SEVs were analyzed for size and number by nanoparticle tracking (ZetaView ...
-
bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
-
bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
-
bioRxiv - Microbiology 2023Quote: The amplicons obtained with each of the strategies (using pools 1 and 2) were pooled in each group and purified with one volume of AMPure XP beads (Beckman Coulter, Brea, CA, USA). The beads were washed with 80% ethanol ...
-
bioRxiv - Cancer Biology 2021Quote: ... decrosslinked material was purified with 2 x AMPure XP beads (Beckman Coulter, Cat #A63881) and eluted in 30 μL of 10 mM Tris-Cl ...
-
bioRxiv - Neuroscience 2020Quote: ... DRG were then centrifuged for 2 min at 1000 rpm (Beckman Allegra X15R, 233xg), supernatant aspirated ...
-
bioRxiv - Molecular Biology 2020Quote: ... and centrifuged for 2 hours at 55,000 rpm (TLS-55 rotor, Beckman Coulter, USA). 11x 200 μl fractions were harvested and subjected to FLAG-IP ...
-
bioRxiv - Cancer Biology 2021Quote: ... resuspended in 300μl PBS/2%FBS and FACS analysed on Gallios® (Beckman Coulter) or ARIAIII (BD) ...
-
bioRxiv - Biophysics 2023Quote: ... 2 mM DTT) with 0.7 M sucrose in Type 45 Ti tubes (Beckman-Coulter). Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg ...
-
bioRxiv - Cancer Biology 2023Quote: ... concentrated for 2 h by ultracentrifugation in swing-out rotor SW-32Ti (Beckman Coulter), 26,000g ...
-
bioRxiv - Physiology 2023Quote: ... Samples were ultracentrifuged at 100,000 rpm at 16°C for 2 hours (rotor: Beckman TLA-100 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Illumina RNASeq sequencing (HiSeq 2000, 2 × 75 pb) and trimming were performed by Beckman Coulter Genomics ...
-
bioRxiv - Molecular Biology 2023Quote: ... and ultra-centrifuged for 2 hours at 20,000 rpm in an SW28 rotor (Beckman). Viral pellets were resuspended in 250 µL PBS and flash-frozen ...
-
bioRxiv - Systems Biology 2023Quote: ... Adapter ligation was followed by 2 successive Agencourt AMPure XP bead purifications (Beckman Coulter) and library amplification by a Plasmodium-optimized KAPA PCR amplication protocol ...
-
bioRxiv - Microbiology 2024Quote: ... and 2 mM dithiothreitol (DTT)) and centrifuged in an SW-41 Ti rotor (Beckman) for 2 h at 40,000 rpm ...
-
bioRxiv - Molecular Biology 2024Quote: ... PCR-2 product was cleaned up with Ampure XP bead-based reagent (Beckman Coulter). Library concentration and purity was verified using the Qubit dsDNA HS kit (Invitrogen ...
-
bioRxiv - Genomics 2024Quote: ... DNA was cleaned up and concentrated with 2× AMPure XP beads (Beckman Coulter; A63882). DNA was eluted in 30µL of Buffer EB (Qiagen ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-CD90 (IM1839U / Beckman Coulter / F15-42-1-5), anti-CD146 (A07483 / Beckman Coulter / TEA1-34) ...
-
bioRxiv - Cell Biology 2022Quote: ... 4°C for 1 hour (JA 25.5 rotor, Beckman Coulter). The supernatant was kept and clarified by a 0.45 µM syringe filter (Pall Corporation) ...
-
bioRxiv - Cell Biology 2021Quote: ... The post-nuclear supernatants were ultracentrifuged using SW41Ti rotor at 100,000 g for 3 h at 4 °C on Optima L-90K (Beckman Coulter). The fraction between 0.4–0.5 M sucrose was collected ...
-
ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... UV-inactive SARS-CoV-2 was then partially-purified using a 20% sucrose cushion centrifuged at 175,000 x g for 3-4 hr using SW32Ti rotor (Beckman Coulter). The sample that has passed through the sucrose was collected and concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore) ...
-
bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 4°C for 3 h at 100,000 g by using Avanti J-30XP (Beckman, USA), and the fractions were collected using a Brandel Fractionation System (USA ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The gradients were ultra-centrifuged at 41,000 rpm for 3 hours at 4°C (Optima L-80 XP ultracentrifuge (Beckman-Coulter)) ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
-
bioRxiv - Molecular Biology 2023Quote: 8 mL of viral supernatant on a 3 mL of 20% sucrose cushion were ultracentrifugated for 3 h at 100,000 x g at 4°C using an Optima L80-XP instrument (Beckman Coulter). After ultracentrifugation ...
-
bioRxiv - Microbiology 2023Quote: ... Each tube was centrifuged for 3 hours at 150’000 x g (4°C) using an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW 32 Ti swinging bucket (369650 ...
-
bioRxiv - Cell Biology 2023Quote: ... The obtained supernatant was layered onto 30 w/v% sucrose solution in ultracentrifugation tubes and subjected to ultracentrifugation at 100,000 × g for 3 h at 4°C using SW32Ti rotor (Beckman courter). The collected sucrose solutions were mixed with PBS and washed by ultracentrifugation at 100,000 × g for 1 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... The gradients were ultra-centrifuged for 3 h at 175,000g at 4°C in a SW41Ti rotor (Beckman-Coulter, USA) and analyzed by measuring the absorbance continuously at 260 nm using a piston gradient fractionator (Biocomp Instruments) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and spun at 40,000 rpm for 3 hours at 4°C using a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter). Sucrose gradient fractionation was performed by using a Brandel Density Gradient Fractionation System ...
-
bioRxiv - Biochemistry 2023Quote: ... The sucrose density gradient was centrifuged at 50,000 rpm for 3 hours at 4°C in a TLS-55 swinging bucket rotor (Beckman Coulter) with minimum acceleration up to 5,000 RPM and deceleration with no break ...
-
bioRxiv - Bioengineering 2023Quote: ... For performing cryogenic transmission electron microscopic imaging EVs were centrifuged and washed in PBS at 100,000 x g for 3 hours at 4°C in a SW41 Ti rotor (Beckman Coulter).
-
bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
-
bioRxiv - Microbiology 2022Quote: ... 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter, USA) through a 20% (w/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... using 10-40 % sucrose gradients in buffer A after 3 hours of centrifugation at 35,000 rpm and 4 °C in SW41 rotor (Beckman Coulter). To isolate ribosomes for structural analysis ...
-
bioRxiv - Molecular Biology 2024Quote: ... The gradient was centrifuged at 54,000 rpm for 3 h at 4 °C using a SW55 Ti rotor (Beckman Coulter). After centrifugation ...
-
bioRxiv - Biochemistry 2024Quote: ... Equivalent amounts of cell lysates prepared as described above were loaded onto the sucrose gradients and spun at 35,000 g for 3 hours at 4°C in a SW41 rotor (Beckman Coulter). After centrifugation the polysomes were fractionated and absorbance was monitored at 254 nm for RNA and 680 nm for GFP using the TriaxTM Flowcell (BioComp Instruments) ...
-
bioRxiv - Neuroscience 2023Quote: ... DNA was purified by phenol/chloroform/isoamyl alcohol followed by SPRI select beads (Beckman Coulter) and DNA concentration was measured with Qubit fluorometer (Thermo Fisher).
-
bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
-
bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min. at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
-
bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min. at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 mM Mg(OAc)2 and 150 mM KCl) and centrifuged at 116,000 xg for 5 h at 4°C in a TL100.3 rotor (Beckman). The ribosome pellet was resuspended by orbital shaking (1 h at 4°C ...
-
bioRxiv - Genomics 2024Quote: ... by centrifuging the chip facing downwards at 3,220 g for 5 min at 4 °C and purified with 1.5 × Ampure XP beads (Beckman), followed by DNA QC and trace checking.
-
bioRxiv - Synthetic Biology 2021Quote: ... 72C for 2’) and a X0.65 SPRI bead DNA clean up (AMPure XP, Beckman Coulter) a second PCR using a p5 primer (F6 ...
-
bioRxiv - Systems Biology 2022Quote: ... Final libraries were size-selected by performing 2-step bead cleaning (SPRIselect, Beckman Coulter B23318) to remove larger DNA fragments and primer dimers and quality was assessed by Bioanalyzer (Agilent 2100 ...
-
bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter).
-
bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). FACS data were analyzed using FlowJo™ v10.8 (BD Biosciences).