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Citations for Beckman :
1951 - 2000 of 2068 citations for 6 9 Methano 4 1 benzoxazepin 2 3H one octahydro 3 methyl 3 α 5a bta 6 bta 9 bta 9a bta 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... and purified according to the previously mentioned bead clean-up procedure (using 0.6 to 1-fold AMPure XP bead suspension, A63880, Beckman Coulter), except that after removal of ethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... Transposition was blocked by the addition of 1% SDS and DNA purified with Agencourt AMPure XP beads (Beckman Coulter, A63881) according to the manufacturer’s instructions using a 2:1 ratio of beads to sample ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2024Quote: ... Additional aliquots of seawater taken from the same CTD casts used for cell-specific rate incubations were 1) preserved in paraformaldehyde (0.5% final concentration) for later shore-based analyses on a CytoFlex S flow cytometer (Beckman Coulter) after staining with Hoechst 34580 (Selph 2021) ...
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Cell Biology 2023Quote: A sucrose gradient was prepared by mixing 3 ml of liver homogenate with 1 ml of HLM containing 60% sucrose in a 13.2 ml ultracentrifuge tube (Beckman Coulter), followed by 5 ml and then 4 ml of HLM buffer containing 5% and 0% sucrose ...
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bioRxiv - Microbiology 2023Quote: ... Not solubilised protein was removed by a second pelleting at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The solubilized and denatured protein was loaded on a 5 ml HisTrap HP column (Cytiva) ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was sonicated 20 times with 1 sec pulses and then centrifuged using a SW55 Ti rotor (Beckman Coulter) at 42,700 rpm for 15 min at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were purified using a ratio of 1:0.85 sample to bead ratio using Agencourt AMPure XP SPRI beads (Beckman Coulter). The final products were quantified on a D5000 Tapestation (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was separated into soluble and insoluble fractions by ultracentrifugation at 100,000 x g for 1 h using Optima L-XP (Beckman Coulter). The soluble fraction was filtered using a bottle-top 0.22-µm filter (Corning) ...
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bioRxiv - Microbiology 2023Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA, 1:100 dilution). Flow cytometry measurements were performed using a FACSCalibur (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 1 hr at RT before centrifugation at 100,000 x g (23°C) in a TLA100 rotor (Beckman-Coulter). The top 50% of the supernatant was removed ...
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bioRxiv - Cell Biology 2023Quote: ... tared and ultracentrifuged for 1 h at 110,000x g using a Beckman L7-65 ultracentrifuge with a SW41Ti rotor (#331362, Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Illumina 16S Metagenomic Sequencing Library Preparation Protocol with the following changes: 10 μL of Amplicon product was purified using a 1:0.8 ratio of XP beads (Beckman Coulter), with beads resuspended in a final volume of 10 μL of nuclease-free water ...
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bioRxiv - Biochemistry 2024Quote: ... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of productively infected cells (p24+ ...
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bioRxiv - Immunology 2024Quote: ... and stained for Gag p24 protein with anti-HIV-1 core antigen monoclonal antibody (clone KC57, Beckman Coulter, cat. 6604665).
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bioRxiv - Synthetic Biology 2024Quote: ... then approximately 1/10th of the volume of each pool was purified with a 0.9:1 ratio of AMPure XP beads:PCR mix (Beckman Coulter, A63881). The complete volume of the AMPure-cleaned 1st round PCR products for each library was used as the templates for a “2nd round PCR,” which added the remainder of the TruSeq HT i5 and i7 Illumina adaptor sequences ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We performed daily 1:210 dilutions of populations in YPD 30°C and SC 30°C and daily 1:28 dilutions of populations in SC 37°C using a BiomekFXp robot (Beckman Coulter). Before dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Subsequently libraries were subject to size selection to exclude DNA fragments larger than 1 kb using SPRIselect magnetic beads (Beckman Coulter). Samples were sequenced on a Illumina HiSeq 4000 platform to obtain 50 bp paired-end reads ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with fluorophore-conjugated antibodies for 30min (see Supplementary Table 1) and FACS-sorted using MoFlo Legacy or Astrios cell sorters (Beckman Coulter). Cells were sequentially gated based on size and granularity (forward scatter ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were analysed by electrophoresis in a 1% (w/v) agarose gel and purified using magnetic AMPure XP beads (Beckman Coulter), using a ratio of beads:PCR product of 2:1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 mM TCEP added before use) and centrifuged for 24 h at 28,000 rpm in a 70Ti rotor (Beckman Coulter, Inc.). The pellets were suspended in washing buffer (20 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... stained cells were resuspended in 1% paraformaldehyde (PFA) and acquired using Stained cells were acquired on Cytoflex (Beckman Coulter, Brea, CA) and analysed with FlowJo v10.0.8 (Tree Star ...
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bioRxiv - Microbiology 2019Quote: ... Then membranes were pelleted by ultracentrifugation at 49,000 x g for 1 hour using a TLA-100.3 rotor in an Optima TL Ultracentrifuge (Beckman Coulter, Indianapolis, IN), and the supernatant was collected for the soluble fraction ...
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bioRxiv - Cell Biology 2019Quote: The concentration of prey and THP-1 cells were measured prior to phagocytosis by flow cytometry (Accuri C6, BD or CytoFLEX, Beckman-Coulter), if needed ...
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bioRxiv - Biochemistry 2020Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 g for 1 hour (Beckman Coulter JA- 20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were diluted in 1 mL and loaded onto a cytoflex-LX flow cytometer (Beckman Coulter, Brea, CA, USA) with a 375 nm laser ...
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bioRxiv - Molecular Biology 2020Quote: ... sucrose cushion prepared in PSB with 100 nM CaCl2 and spun for 1 hour at 55,000 rpm (TLS-55 rotor, Beckman Coulter, USA) to remove aggregates ...
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bioRxiv - Microbiology 2019Quote: ... Viruses were then concentrated 50-fold by ultracentrifugation over a sucrose cushion at 23,000RPM for 1 hour at 4C using a SW28 rotor (Beckman Coulter; 342207). Concentrated virus was re-suspended in D10 media ...
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Cancer Biology 2020Quote: ... The emulsions were then broken using perfluoro-1-octanol and the aqueous fraction was diluted in water and collected for DNA purification with SPRI beads (Beckman Coulter). Sample indexes and Illumina adaptor sequences were then added via a 10 cycle PCR reaction and the amplified material was then SPRI purified a second time ...
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bioRxiv - Microbiology 2022Quote: ... and ½ of each plug was inserted into a well of a 1% LE agarose gel (Beckman Instruments, Inc., Palo Alto, CA) and electrophoresed on a CHEF Mapper® XA Pulsed-Field Gel Electrophoresis System (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... Thylakoid membranes were extracted via ultracentrifugation at 24,000 rpm for 1 h and 20 min (Beckman Coulter SW 32 Ti rotor). Thylakoids were collected from the step gradient interphases with a Pasteur pipet ...
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bioRxiv - Microbiology 2022Quote: ... The PCR templates were purified using Agencourt AMPure XP (PCR product: AMPure XP beads = 1:0.8; Beckman Coulter, Brea, California, USA) before the second PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... 2D10 cells were washed twice with 1 x phosphate buffered saline (PBS) and analyzed by flow cytometer (Epics Altra, BECKMAN COULTER) for the percentages of cells expressing GFP.
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bioRxiv - Molecular Biology 2022Quote: ... Samples were tagged using barcodes NB01 to NB05 of the Native Barcoding Expansion 1-12 PCR-free kit (EXP-NBD104, ONT) and purified using Agencourt AMPure XP beads (Beckman Coulter). The barcoded samples were quantified using a Qubit fluorometer and pooled equimolarly to reach 700 ng in 65 μL ...
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bioRxiv - Immunology 2022Quote: ... Samples are washed with 1X FACS buffer again to remove the PFA and finally re-suspended in 1 x FACS buffer for flow cytometry analysis using either Fortessa instrument (Becton Dickinson) or CytoFlex LX instrument (Beckman Coulter) fitted with an automated sampler accommodating 96-well plates ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (23 ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Cell Biology 2022Quote: Live Jurkat E6.1 cells were stained with Laurdan dye as detailed above and analyzed by flow cytometry using a Cytoflex S (Beckman Coulter). Cells were resuspended in HBSS without Ca2+ and Mg2+ (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin-V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (73 ...
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with permeabilization buffer and dissolved in FACS buffer (PBS, 1% BSA, 2mM EDTA) for analysis using a CytoFLEX S flow cytometer (Beckman Coulter).
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bioRxiv - Developmental Biology 2019Quote: ... with a final double (0.65 x −1 x bead volume) size selection step using a SPRIselect reagent kit (Beckman Coulter, B23318). The libraries were indexed (New England BioLabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Exosome pellets were resuspended in phosphate-buffered saline (PBS) and centrifuged at 27300 rpm for 1 hour (SW60Ti rotor; Beckman Coulter). The exosome pellets were resuspended in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... The complete NanoTrap was purified using rate-zonal centrifugation33 through a 1545% glycerol gradient in 1×TE + 10 mM MgCl2 in an SW 55 rotor (Beckman Coulter). Fractions were collected after a 1 hr centrifugation at 50 k rpm ...
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Neuroscience 2019Quote: ... Catalog L3224A) and lack of EtD-1 homodimer staining (L3224B) were selected and sorted by a MoFlo Astrios cell sorter (Beckman Coulter) into 500 µL filled into dissecting medium.
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were then filtered through 40 μm Falcon™ Cell Strainers (Thermo Fischer Scientific, cat no. 08-771-1) and analyzed by flow cytometry (MACSQuant VYB, Miltenyi or CytoFLEX, Beckman Coulter). Matlab-based software for processing flow cytometry data was developed in-house by Yaron Antebi.
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bioRxiv - Cancer Biology 2021Quote: ... the 197 library compounds dissolved in DMSO or MilliQ (Supplementary Table 1) were added in duplicate using the Echo 550 dispenser (Beckman Coulter). Final drug concentrations ranged standard between 0.1 nM and 10 μM ...