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Citations for Beckman :
151 - 200 of 364 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The ribosomes were further purified by centrifugation at 59,727 ×g (15 hours, 4 °C, SW 32.1 Ti rotor, Optima™ L-100 XP, Beckman Coulter) in a linear (15 – 30 % ...
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bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22,000 rpm for 2 hours at 4°C with OptimaTM L-90K Ultracentrifuge (Beckman Coulter) and stored at -80°C (never refrozen).
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bioRxiv - Neuroscience 2023Quote: ... The lysate was separated into soluble and insoluble fractions by ultracentrifugation at 100,000 x g for 1 h using Optima L-XP (Beckman Coulter). The soluble fraction was filtered using a bottle-top 0.22-µm filter (Corning) ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter; OptimaTM L-90K, Beckman Coulter). After the ultracentrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... and then centrifuged for 150 min at 39,000 rpm and 4°C in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41Ti rotor ...
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bioRxiv - Cell Biology 2023Quote: ... The gradient was centrifuged at 4 °C and 100,000 ˗ g for 18 h (Optima L-80 XP Ultracentrifuge with Swing Rotor (Beckman Coulter)) ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were sedimented by velocity centrifugation at 36,000 x rpm for 2 h at 4°C using SW40Ti rotor in Optima L-80XP ultracentrifuge (Beckman Coulter). Absorbance at 254 nm was measured from lower to higher sucrose gradients using an ISCO gradient fractionation system and the optical density at 254 nm was continuously recorded with a Foxy JR Fractionator (Teledyne ISCO).
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bioRxiv - Cell Biology 2024Quote: ... The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose) and centrifuged at 190,000 g for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Extracts were incubated with pre-washed anti-Flag beads (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22.000 rpm for 2h at 4°C with OptimaTM L-90K ultracentrifuge (Beckman Coulter) and stored at −80°C (never refrozen).
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bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing the membrane fraction was transferred to Beckman ultracentrifuge tubes and subjected to ultracentrifugation for 2 h at 41,000 r.p.m (Beckman Optima L-100 XP ultracentrifuge, Beckman 45 Ti rotor). Membrane pellets were resuspended to a total protein concentration of 3.5 mg/mL in ice cold 1 x PBS using the Bicinchoninic Acid (BCA ...
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bioRxiv - Microbiology 2020Quote: ... 3′ ligated RNA was cleaned by incubating with 2.5X volume of RNAClean XP beads (Beckman Coulter) and 1.5X volume of isopropanol for 15 min ...
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bioRxiv - Neuroscience 2019Quote: ... and 3% Percoll (1 ml each) in Polycarbonate centrifuge tubes (13 x 51 mm; Beckman Coulter). The gradients were then centrifuged for 15 min at 18,700 rcf ...
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bioRxiv - Biophysics 2020Quote: ... After centrifugation at 30,000 rpm (4 ° C for 3 h) in the SW45Ti rotor (Beckman Instruments), eight 1.5-ml fractions were collected from the top of a gradient ...
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bioRxiv - Biochemistry 2020Quote: ... Gradients were resolved at 36,000 rpm for 3 hours at 4 °C in SW41 rotor (Beckman). Both separation and fractionation of gradients used a Biocomp Gradient Station (BioComp Instruments ...
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bioRxiv - Biochemistry 2020Quote: ... subjected to centrifugation (36,000 rpm for 3 hours at 4 °C, SW-41Ti Beckman Coulter rotor) and analysed using Biocomp Gradient Station (BioComp Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were counted using the electrical sensing zone method (Multisizer 3, Beckman Coulter, Indianapolis, IN)
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bioRxiv - Microbiology 2023Quote: ... 3′ ligated RNA was cleaned by incubating with 2.5X volume of RNAClean XP beads (Beckman Coulter) and 1.5X volume of isopropanol for 15 min before separation on a magnetic rack ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase 3 and 8 activity (EGFP+ cells) was analyzed using the CytoFLEX Flow Cytometer (Beckman Coulter) and Flowjo v10 BD (Becton Dickinson ...
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bioRxiv - Cell Biology 2023Quote: ... Cell density and sizing was measured with a Coulter counter (Beckman Coulter Multisizer 3, CA, USA). Raw cell diameter event counts were converted to relative frequency and visualized as ridgeline plots with the R tidyverse packages (https://tidyverse.tidyverse.org/ ...
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bioRxiv - Cell Biology 2019Quote: ... by ultracentrifugation at 150,000 × g for 12 h at 4°C using an Optima L-100XP centrifuge equipped with a SW55Ti rotor (Beckman Coulter). Eleven fractions were collected from the top of the sucrose gradient.
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bioRxiv - Molecular Biology 2021Quote: ... About 1,5 mg of protein was loaded on a 10–50% sucrose gradient and centrifuged for 2h45min at 39 000 rpm in an Optima L-100XP Ultracentrifuge (Beckman-Coulter) using a SW41 rotor (Beckman) ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Full hematology with blood cell count (CBC) was performed at each time of bleeding using an HMX A/L (Beckman Coulter). Immunological investigations were performed with fresh whole blood or isolated peripheral blood mononuclear cells (PBMCs).
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bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Each pool of samples was then purified using 79 μL beads buffer (20% PEG-8000 in 2.5 M NaCL, final concentrations) and 50 μL Ampure XP Beads (Beckman Coulter, A63881), and eluted in 7 μL NF H2O.
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bioRxiv - Cell Biology 2021Quote: ... Sample solution containing EVs was placed at the top of the tube (at RT) and ultracentrifugation (Beckman Optima L-90K Ultracentrifuge) was performed at 40,000 RPM for 16 hours at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The 1 ml concentrates were overlaid on top of the gradient, and centrifuged at 100 000 g (28 000 rpm, k-factor 266) in an Optima L-90K ultracentrifuge (Beckman Coulter) using an SW41 Ti rotor for 18 h at 4 °C with slow deacceleration ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 100,000 g at 4°C for 60 min in a swinging rotor (SW40Ti) (Beckman Coulter OptimaTM L–100K). Following centrifugation ...
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bioRxiv - Biochemistry 2019Quote: A 150 L aliquot of the fibrillation sample was centrifuged for 60min at 100,000 x g (Beckman Coulter Optima MAX-XP) to precipitate the fibrils ...
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bioRxiv - Immunology 2021Quote: ... Samples were spun at 76,650 x g for 2 hours at 4° C inside a SW 32i rotor using an Optima L-90K Ultracentrifuge (Beckman Coulter). Purified virus was quantified using a bicinchoninic acid assay (BCA ...
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bioRxiv - Genomics 2021Quote: ... The remaining 20 μL of each pool were pooled and clean-up was performed using an equal volume of AMPure XP beads (Beckman Coulter) and quantified using 1 μL of sample with the Qubit dsDNA HS Assay Kit on a Qubit 2.0 Fluorometer ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was then recovered and centrifuged at 30,000 gav for one hour in the Ti45 rotor and in a Beckman L-80 ultracentrifuge (Beckman-Coulter, Mexico). The resulting pellet was re-suspended in 5 mL of modified CIB medium and centrifuged at 16,000 gav for 10 min in 5415R refrigerated centrifuge (Eppendorf ...
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bioRxiv - Physiology 2022Quote: ... Labeled vesicles were then washed in PBS and ultracentrifuged at 100 000 x g for 70 min (SW 40 Ti Swinging-Bucket Rotor; Optima L-100 XP Ultracentrifuge; Beckman Coulter). Next ...
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bioRxiv - Physiology 2022Quote: ... 40 ml of filtrates were ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 x g for 70 min at 4°C using an Optima L-100 XP Ultracentrifuge equipped with 90 Ti Fixed-Angle Titanium Rotor (Beckman Coulter). The final EVs pellets were suspended in 200 µl of PBS (Lonza) ...
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bioRxiv - Microbiology 2022Quote: ... 50 μL of the amplified ATAC-seq library was subjected to double-sided size selection using magnetic beads (AMPure XP, Beckman Coulter). DNA bound to the beads was washed twice with 80% ethanol and then eluted in 20 μL H2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting complexes were either used for structural analysis or resolved on a 15 mL 10-40% sucrose density gradient centrifuged at 35000 rpm for 135 minutes using SW41 rotor on an Optima L-90K ultracentrifuge (Beckman Coulter), and the resolved particles were fractionated as previously described (59) ...
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bioRxiv - Physiology 2023Quote: ... was ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 × g for 70 min at 4°C (Optima L-100 XP Ultracentrifuge, 90 Ti Fixed-Angle Titanium Rotor; Beckman Coulter). The final EV pellet was suspended in 50 µl of PBS without Ca2+ and Mg2+ (Lonza ...
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Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in SynechocystisbioRxiv - Synthetic Biology 2023Quote: ... Ultracentrifugation was performed in a SW40 Ti swinging-bucket rotor at 130000 × g for 16 h at 20 °C (Optima L-80 XP, Beckman Coulter).
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bioRxiv - Bioengineering 2023Quote: ... Samples were ultracentrifuged at 100,000×g for 70 min at 4°C using the Beckman OptimaTM L-80 XP ultracentrifuge (Beckman Coulter, CA). The supernatant was discarded ...
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... The pre-concentrated culture was centrifuged (10000 g, 10 min) in 1-L buckets (Avanti J-265 XP, Beckman Coulter, USA). The biomass reached a concentration of approximately 40 % (dry weight - DW ...
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bioRxiv - Cell Biology 2019Quote: ... and centrifuged at 200,000 g for 3 h at 4°C in a TLS55 rotor (Beckman Coulter). The sucrose gradient was fractionated manually from the top ...
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bioRxiv - Biochemistry 2020Quote: ... One was through twice daily measurement of 2 or 3 levels of QC specimens obtained from Beckman Coulter Inc ...
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bioRxiv - Cell Biology 2021Quote: The average cell density in a population was measured by comparing Multisizer 3 Coulter Counter (Beckman Coulter)-based cell volume measurements and suspended microchannel resonator (SMR)-based buoyant mass measurements ...
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bioRxiv - Plant Biology 2022Quote: ... The number of intact cells were measured using a Coulter Counter (Multisizer 3, Beckman Counter, Brea, CA).
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bioRxiv - Cancer Biology 2022Quote: ... the genomic DNA was cleaned up with AMPure XP beads at 3:1 ratio (Beckman Coulter A63880) and eluted in the 10 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were analyzed using a 3-laser Beckman Coulter Gallios using Kaluza Software (Beckman Coulter, Brea, CA).
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bioRxiv - Neuroscience 2020Quote: ... Samples were analyzed with a 3-laser Beckman Coulter Gallios using Kaluza Software (Beckman Coulter, Brea, CA).
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bioRxiv - Genetics 2020Quote: ... (3) a 1x post ligation bead cleanup with AMPure XP beads (Beckman Coulter Life Sciences, IN, USA) was performed and (4 ...