Labshake search
Citations for Beckman :
151 - 200 of 1496 citations for Mouse Anti Hepatitis C Virus NS4a b Antibody 1865 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Permeabilized cells were then incubated with 100 μl of 1:100 dilution of phycoerythrin-conjugated mouse anti-p24 mAb (KC57-RD1; Beckman Coulter, Inc.) for 30 min at 4 °C with intermittent mixing ...
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bioRxiv - Biochemistry 2019Quote: ... low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C) using commercially-available kits (Beckman-Coulter, CITY, STATE, USA). Serum insulin concentration was determined using an electrochemiluminescence-based assay (Immulite 2000 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated further overnight at 20°C and subsequently harvested by centrifugation (Beckman rotor 8.1000, 5000 rpm, 15 min, 4°C). The pellet was resuspended in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with the fluorescently labeled anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution).
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bioRxiv - Microbiology 2020Quote: ... 4°C in SW 60 Ti rotor (Beckman Coulter) to pellet cyanophage particles ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (SW32Ti rotor, Optima L-80XP ultracentrifuge, Beckman) with the maximum acceleration and minimum deceleration ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C in swinging-bucket rotor SW40 (Beckman Coulter). Fractions (1 ml ...
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bioRxiv - Systems Biology 2019Quote: ... at 4°C using Beckman SW55 rotor (Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C cells were resuspended in Isoflow (Beckman) for further reading the percentage of fluorescent labelled cells on the cytometer (FC500 ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C (SW41 Ti Swinging-Bucket rotor, Beckman & Coulter). Bands containing virus particles were extracted with a syringe ...
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bioRxiv - Molecular Biology 2022Quote: ... for 45 min at 10°C (45Ti/Beckman Coulter). The pellet was re-dissolved in lysis buffer ...
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bioRxiv - Microbiology 2023Quote: ... 30 °C (TLA-100.3 rotor; OptimaTM Max ultracentrifuge, Beckman) and resuspended in Milli-Q water ...
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bioRxiv - Microbiology 2021Quote: ... and stained for 30min at room temperature with 1:160 dilution of PE-conjugated mouse-anti-human Ab to HIV-1 p24 and core antigens (Beckman Coulter, Fullerton, CA). Fluorescent profiles were recorded using a Guava easyCyte and InCyte software.
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bioRxiv - Neuroscience 2021Quote: ... 30s at 60°C and 60s at 68°C) and DNA was cleaned and purified using magnetic SPRI beads (Beckman Coulter, Ca# B23317). Libraries were resuspended in 25μl Low EDTA TE buffer and concentration was measured using a Qubit dsDNA HS Assay Kit ...
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bioRxiv - Cell Biology 2023Quote: ... IgG2a Mouse-PE (A09142, Beckman Coulter), IgG1κ Mouse-AF647 (565571 BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... Transfectants were stained for viability using a Live dead blue dye followed by anti-HLA DR DP DQ antibodies (BioRad clone Bu26, Cat. #MCA2497F; Beckman clone I3 ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to an ultracentrifuge tube and overlayed with layers of Lysis Buffer A with 12% Ficoll and Buffer B with 8% Ficoll (AAB2209518, Fisher) and centrifuged at 28,500 rpm for 45 min (Beckman 42.1). Afterwards ...
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bioRxiv - Microbiology 2020Quote: We stained and sorted single memory B cells as previously described29 using a MoFlo Astrios EQ Cell Sorter (Beckman Coulter). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: ... 10,000 × g (30 minutes at 4°C) and 100,000 × g (120 minutes at 4°C) by using a Beckman ultracentrifuge (AvantiJ-30I; Beckman Coulter, Inc., Brea, CA, USA). The final sediment was resuspended in the ExoQuick-TC exosome precipitation solution (System Biosciences ...
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bioRxiv - Molecular Biology 2019Quote: ... 20°C in a GH-3.8 swinging bucket rotor (Beckman). Quiescent cells were collected with a Pasteur pipette and washed once with 30 ml of 50 mM Tris-HCl pH 7.5 at 650 g ...
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bioRxiv - Biophysics 2021Quote: ... centrifuged (Beckman Avanti, 1500 x g, 10 min, 4 °C) and pellets were flash-frozen and stored at −80 °C.
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bioRxiv - Cell Biology 2021Quote: ... for 5 minutes at 4°C with TLA100 rotor (Beckman). 100 µg cell lysate was brought to a final volume of 250 µl BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Immunology 2022Quote: ... for 90 minutes at 4°C (Optima XE-90, Beckman-Coulter with SW 32 Ti swinging bucket rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... and ultracentrifuged (60,000 rpm, 4 °C, Beckman, 70 Ti rotor) for 20 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 4°C for 1 hour (JA 25.5 rotor, Beckman Coulter). The supernatant was kept and clarified by a 0.45 µM syringe filter (Pall Corporation) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... using a DelsaNano C Particle Analyzer (Beckman Coulter, Krefeld, Germany). The CONTIN method was used to calculate the hydrodynamic diameter of the particles ...
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bioRxiv - Molecular Biology 2020Quote: ... at 50.000 rpm and 11 °C (Beckman SW65 Ti Rotor). The virion band was extracted using an 18‐ gauge needle syringe ...
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bioRxiv - Synthetic Biology 2020Quote: ... After clarification (20,000 rpm, Beckman JA 25.5, 30min 4°C), lysate was incubated with Glutathione S-sepharose 4B resin (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... Following centrifugation (Beckman SW40 rotor, 105,400xg, 18 h, 4°C), the heavier green band was collected and precipitated using 8% PEG6000 (Hampton Research ...
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bioRxiv - Immunology 2020Quote: ... for 90 minutes at 4°C (Optima XE-90, Beckman-Coulter with SW 32 Ti swinging bucket rotor ...
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bioRxiv - Cell Biology 2022Quote: ... at 4°C in a TLA-55 rotor (Beckman Coulter) at maximum speed (186000 g ...
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bioRxiv - Biochemistry 2023Quote: ... 4 °C (Fixed angle Ti90 Rotor, Optima XE90, Beckman Coulter). The supernatant was loaded onto a 70 ml Q-sepharose column (XK 26/20 ...
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bioRxiv - Neuroscience 2024Quote: ... Analysis was performed using Kaluza C Analysis software (Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... NaCl solution was added to 1 M and the solution was centrifuged for 10 min at 12,000 × g at 1°C and for 20 min at 38,000 x g at 1°C (Beckman Coulter Avanti J-25I, JA 25.50 rotor). CHAPS was added to 0.02% ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were resuspended in 200 μl and stained on ice 30 minutes with APC conjugated anti-CD56 antibody (Beckman Coulter) and subsequently washed ...
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bioRxiv - Cancer Biology 2019Quote: Cells were stained for 30 min (minutes) with the anti-human monoclonal antibody CD11b-PE (IM2581U, Beckman Coulter, Brea, CA, USA), CD14-FITC (555397 ...
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Microbiology 2020Quote: ... Duplicates of samples without EdU were analysed by FACS and stained in permeabilized samples with 0.05% Saponin using anti-Gag antibody conjugated to Rhodamine (Beckman Coulter, #6604667) diluted 1:500 in PBS-BSA 0.5% ...
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bioRxiv - Immunology 2022Quote: ... and the expression of the Gag- p24 viral protein was detected with a PE-anti-p24 antibody (clone KC57 RD1; Beckman Coulter). Cell viability was determined using an aqua viability dye for flow cytometry (LIVE/DEAD® Fixable Aqua Dead Cell Stain kit ...
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bioRxiv - Immunology 2022Quote: ... cells were seeded into pre-coated wells with anti-TCR pan-γδ clone IMMU510 antibody (5ug/ml) (Beckman Coulter, Krefeld, Germany) and IL-2 (100IU/ml) ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Immunology 2019Quote: ... Naïve B cells (CD19+ CD27− IgD+) and unswitched memory B cells (CD19+ CD27+ IgD+) were obtained by FACS sorting on a MoFlo Astrios (Beckman Coulter). Purified samples were pelleted and stored at −80°C.
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bioRxiv - Immunology 2020Quote: ... B- and NK cells within the lymphocyte population was performed by a whole blood staining lyse-no-wash protocol (Optilyse B, Beckman-Coulter) using six colour flow cytometry with fluorochrome-conjugated antibodies as listed in Supplementary Table 7 top ...
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bioRxiv - Cell Biology 2022Quote: ... and the pellet was resuspended with 5 ml buffer B and transferred into a 14 ml ultracentrifuge tube (331372, Beckman Coulter). 9 ml Buffer C (1.8 M sucrose ...
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bioRxiv - Biochemistry 2023Quote: ... and 400 μL was loaded onto a 5-30% (w/v) buffered sucrose gradient (in Buffer B) in a 14 mm × 89 mm thin-wall Ultra-Clear tube (Beckman # 344059) that was formed using a Biocomp Gradient Master ...
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bioRxiv - Plant Biology 2023Quote: ... Uptake was terminated by rapid filtering of the cells into the kill solution during 20 s centrifugation at set maximum speed (microfuge B, Beckman, USA). For dark-inactivation experiments ...
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bioRxiv - Biochemistry 2019Quote: ... Resuspended cells were lysed by French Press (8,000 psi, 4°C, 2 passages) and lysates were centrifuged (Beckman 60Ti rotor, 30 min, 50,000 rpm, 4°C). Pellets were resuspended in 100 ml of buffer B (PBS [140 mM NaCl ...
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bioRxiv - Genomics 2020Quote: ... by ultracentrifugation at 4°C overnight (33500 rpm, Beckman, 70Ti rotor). The ribosome pellet was dissolved in 300 μL resuspension buffer (20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biophysics 2019Quote: ... 4 °C for 10 min using TLA-100 rotor (Beckman-Coulter). Tubulin in the supernatant was polymerized by incubating in the dark at 37 °C for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... for 60 min at 4 °C on ultracentrifuge (Beckman L7-65) and rotor (Beckman SW41-Ti) ...