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Citations for Beckman :
151 - 200 of 1517 citations for 4 5 5 5 Tetrafluoro 4 trifluoromethoxy pentan 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The lysates containing matured PsVs were loaded on an OptiPrep gradient that had been stabilized at least 1 h and centrifuged at 50k x g for 3.5-4 h at 4°C in a SW-55Ti rotor (Beckman). Fractions were collected in siliconized tubes and subjected to SDS-PAGE followed by Coomassie blue staining to assess the abundance of L1 and L2 proteins ...
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bioRxiv - Microbiology 2024Quote: ... The lysates containing matured PsVs were loaded on an OptiPrep gradient that had been stabilized at least 1 h and centrifuged at 50k ×g for 3.5-4 h at 4°C in a SW-55Ti rotor (Beckman). Fractions were collected in siliconized tubes and subjected to SDS-PAGE followed by Coomassie blue staining to assess the abundance of L1 and L2 proteins ...
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bioRxiv - Genetics 2024Quote: ... and spun at 70,000 rpm for 4 hours at 4°C using a TLA 100.3 Rotor in an Optima Ultracentrifuge (Beckman, 361889). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... and the remainder was then loaded onto a 20%-60% sucrose cushion and centrifuged at 25,000 rpm at 4°C for another 4 hours using the SW41 rotor (Beckman). The initial 5 μL sample and each sucrose gradient layer were then absorbed ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded on the top of the gradient and centrifuged at 260,000 × g and 4 °C for 2 h using SW 41 rotor (Beckman). Collection of polysomal fractions were done using Piston Gradient Fractionator (BioComp) ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) by being centrifuged at 73000 rpm for 45 minutes at 4°C (Beckman Coulter TL120.2 rotor). The mitoribosomal pellet was subsequently resuspended in ribosome resuspension buffer (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... and centrifuged at 24,000 rpm for 2 h at 4°C using a SW-Ti32 swinging bucket (Beckman Coulter). The upper layer was discarded ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 0.9% NaCl and spun at 110,000 × g for 2 hours at 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Plant Biology 2023Quote: ... Extract was then centrifuged at 40 000 rpm for 2 hours at 4°C using the 50Ti rotor (Beckman). A protocole previsouly used in the laboratory and described in Maclot et al ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 μg of total protein was loaded onto a 10% to 50% sucrose gradient and centrifuged at 36,000 rpm for 2 hours at 4°C using an SW41 rotor (Beckman). Polysome profiles were visualized ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... Fifteen A260 units of WCEs were layered on a pre-chilled 10-50% sucrose gradient and centrifuged at 40,000 rpm for 2 h at 4°C in a SW41Ti rotor (Beckman). Gradient fractions were continuously scanned at A260 using the BioComp Gradient Station and polysome to monosome (P/M ...
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bioRxiv - Cancer Biology 2023Quote: ... the obtained supernatant was filtered through a 0.22 μm filter before ultracentrifugation at 150,000 g for 2 h at 4 °C (Beckman Coulter). The nsEV-containing pellet was resuspended in phosphate buffer saline (PBS).
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... were layered on top of each sucrose gradient and centrifuged (41,000 rpm for 2 hours at 4°C) in an SW41 Ti rotor (Beckman). UV profiling (254 nm ...
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bioRxiv - Immunology 2020Quote: ... and anti-CD27-PE-Cy5 (clone 1A4CD27; 2.5 μL; cat. # 6607107) and anti-NKG2A-APC (clone Z199; 5 μL; cat. # A60797) from Beckman Coulter (Supplementary Fig ...
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bioRxiv - Biochemistry 2020Quote: ... The cultures were grown until OD600 of 0.8 at 37 °C and the cells were collected by centrifugation at 8,000 rpm for 5 minutes in JLA-16.25 rotor (Beckman Coulter), dissolved in 0.5 mL of HEPES:Polymix buffer ...
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bioRxiv - Microbiology 2021Quote: ... liquid-captured bioaerosols (5 – 10 mL) were centrifuged for 10 minutes at 3000 × g (Allegra X-15R, Beckman Coulter) and resuspended in 200 μL of Middlebrook 7H9 medium supplemented with 100 μM DMN-tre ...
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bioRxiv - Cell Biology 2021Quote: ... and the mixtures were ultracentrifuged at 80,000 rpm for 5 min at 30 °C using a TLA100 rotor and an Optima TLX ultracentrifuge (Beckman). The microtubule pellets were resuspended in PEM supplemented with 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 % solutions on top of each other in a 13.2 ml open-top polyallomer tube (331372, Beckman Coulter). The 1 ml concentrates were overlaid on top of the gradient ...
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bioRxiv - Microbiology 2021Quote: ... Linear sucrose gradients were prepared by layering 1.8 ml each of sucrose solutions (wt/wt; prepared in 5 mM EDTA, pH 7.5) into 14-by 95-mm ultracentrifuge tubes (Beckman) in the following order ...
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bioRxiv - Cancer Biology 2020Quote: ... equivalent amounts of ribosomes for each lysate was loaded onto each 10%-45% sucrose density gradient (BioComp Gradient Master machine) for ultracentrifugation at 33,000 rpm for 5 hours (Beckman Optima L80K Ultracentrifuge ...
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bioRxiv - Microbiology 2022Quote: ... Cells were grown for additional 1 hour at 30 °C and the biomass was harvested by centrifugation (10,000 rpm, for 5 minutes, JLA-10.500 rotor (Beckman Coulter)).
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bioRxiv - Genetics 2020Quote: ... 5 μL of each PCR reaction from the single worm lysate was pooled and purified by SPRI (Beckman Coutler) to remove unincorporated oligos ...
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bioRxiv - Immunology 2021Quote: ... Soluble CuMV-CP-containing protein solution was separated from the cellular proteins by ultracentrifugation in a sucrose gradient (20%-60% sucrose; ultracentrifugation at 25,000 rpm for 6 hours at 5°C [SW28 rotor, Beckman]). After dialysis CuMVTT-containing gradient fractions ...
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bioRxiv - Microbiology 2020Quote: ... The sample was vortexed for 10 s and incubated at room temperature for 5 minutes before centrifugation at 12,000 rpm (JA-15 1.5 rotor, Beckman Coulter) 4°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Resuspended pellets were then subjected to an iodixanol density gradient in 5-mL Ultra-Clear centrifuge tubes (Beckman Coulter). The tubes were first filled with a two-layer gradient ...
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (5 mg/ml) and floated for 2h at 55,000 rpm in a TLS-55 rotor (Beckman) at 25 °C through a sucrose gradient ...
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bioRxiv - Cell Biology 2022Quote: ... The stock solution of aggregate-depleted BSA (5% w/v) was prepared by overnight centrifugation at 100,000 x g (SW28 rotor Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... and 3.5 mL of 5% iodixanol in a 16.8 mL open top polyallomer tube (Beckman Coulter, Fullerton, California, USA). One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... respectively) were gently layered on top of each other in ultracentrifuge tubes (Ultra-Clear 5 mL Tube, Beckman Coulter) and a continuous gradient was generated using a gradient maker (Gradient Master ...
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bioRxiv - Microbiology 2024Quote: ... The phage was purified by isopycnic centrifugation in a CsCl discontinuous gradient for 5 h at 32,000 rpm in a SW41 rotor (Beckman) at 20°C ...
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bioRxiv - Neuroscience 2023Quote: ... after which the cells were harvested by centrifugation at 4,000 rpm for 30 min at 5°C using the JLA 8.1000 rotor (Beckman Coulter). The harvested pellets were stored at −20°C until use ...
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bioRxiv - Microbiology 2022Quote: ... the stained cells were detached by 5 mM of EDTA/PBS and analyzed with a CytoFLEX Flow Cytometer (Beckman). 10,000 events in a gated live cell population (based on SSC/FSC ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed by centrifugation at 1,300 g for 5 min (Beckman Centrifuge Avanti® J-26-XP). Afterwards ...
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bioRxiv - Cancer Biology 2023Quote: ... Percentage of cells with each DNA content (2N, 3N, 4N, 5-8N) were estimated using CytExpert software (Beckman Coulter). For DNA content analysis using sorted cells ...
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bioRxiv - Molecular Biology 2024Quote: ... the CP was centrifuged at 72,000 rpm (280,000 × g) for 1 h at 5 °C in a TLA-100.3 rotor (Beckman Coulter, USA) on a TL-100 ultracentrifuge (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... the samples were centrifuged at 72,000 rpm (280,000 × g) for 1 h at 5 °C in a TL-100.1 rotor (Beckman Coulter, USA) on a TL-100 ultracentrifuge to pellet the reassembled VLPs ...
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bioRxiv - Neuroscience 2024Quote: ... but 2.5 mL for 5%) and ultracentrifuged at 100,000 x g (SW 40 Ti or SW41 Ti rotor, Beckman Coulter) for 16 h using ultra-clear tubes (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were subjected to two rounds of centrifugation at 18000 rpm for 5 and 30 mins in a JA-25.50 rotor (Beckman). Protein levels were normalized for coimmunoprecipitation and immunoblot analysis using a Bradford Assay ...
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bioRxiv - Genomics 2024Quote: ... Cell concentration was measured daily (particle size 5-8 um) using a Coulter counter (Coulter Z2, Beckman Coulter GmbH), in all experimental tanks ...
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bioRxiv - Biochemistry 2020Quote: ... which were centrifuged at 50,000rpm for 4 hours at 4°C using a TLS55 rotor and an Optima MAX-XP Ultracentrifuge (Beckman Coulter). The sample was physically fractionated into 10 fractions of 85 uL each ...
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bioRxiv - Molecular Biology 2022Quote: ... 800 µl of EDTA-treated lysate was layered on top of the sucrose gradient and ultracentrifuged at 35000 rpm for 4 hours at 4 °C in a SW41Ti rotor (Beckman). After centrifugation ...