Labshake search
Citations for Beckman :
151 - 200 of 200 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... Equivalent amounts of cell lysates prepared as described above were loaded onto the sucrose gradients and spun at 35,000 g for 3 hours at 4°C in a SW41 rotor (Beckman Coulter). After centrifugation the polysomes were fractionated and absorbance was monitored at 254 nm for RNA and 680 nm for GFP using the TriaxTM Flowcell (BioComp Instruments) ...
-
bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
-
bioRxiv - Genetics 2020Quote: ... PCR products were pooled by region of interest using 2-3 μL of each reaction and the resulting pools of products were purified using a 1X Ampure XP cleanup (Beckman Coulter). Purified libraries were pooled and submitted for paired-end 150 cycle sequencing on an Illumina MiSeq ...
-
bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
-
bioRxiv - Microbiology 2021Quote: ... triplicate spore preparations were made in 3 ml of 20% glycerol and suspended in Isoton II (Beckman Coulter, Brea, CA, USA). The particle volume was then measured for a minimum of 100 000 particles per sample using a Multisizer 4e Coulter counter (Beckman Coulter ...
-
bioRxiv - Cell Biology 2020Quote: ... with densities ranging from 1.10 to 1.15 were pooled and washed in PBS (Beckman Ti50, 3 h at 42 000 rpm).
-
bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were then enriched in DNA sizes between 200 and 600 bp (corresponding to 1 to 3 nucleosomes) using AMPure XP magnetic beads (Beckman-Coulter), following the manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2020Quote: ... 3 ml of 50% and 3 ml of 40% sucrose) in Ultra-Clear Beckman tubes and centrifuged at 26000 rpm (SW32Ti rotor, Beckman Coulter) for 1 h at 4°C ...
-
bioRxiv - Biochemistry 2020Quote: ... and pelleted for 3 min in a swing-out rotor at 1000 rpm in a tabletop centrifuge (Allegra X-30R, Beckman Coulter). The supernatant was aspirated and cells were suspended in the remaining liquid to 1 × 105 – 1 × 107 cells/100 μl ...
-
bioRxiv - Microbiology 2020Quote: ... The supernatant was ultracentrifuged at 120,000 g for 3 h at 4°C using an MLS-50 rotor (Beckman Coulter, USA) to collect 120k pellet vesicles ...
-
bioRxiv - Genetics 2020Quote: ... Timepoints were measured every 2-3 days by flow cytometry analysis of >7,000 cells (either a BD Accuri C6 or Beckman Coulter CytoFLEX). Data was analyzed using Cytoflow (https://github.com/bpteague/cytoflow ...
-
bioRxiv - Biophysics 2021Quote: The aggregation products formed in Eppendorf tubes under agitation conditions at pH 3 and pH 4 were centrifuged using an Optima MAX-XP ultracentrifuge (Beckman Coulter) in a TLA-55 rotor at 40,000 rpm at 20°C for 45 min ...
-
bioRxiv - Genomics 2020Quote: ... We carried out the first round of PCR for 3 cycles and purified the reactions with Ampure XP beads (Beckman-Coulter). We then carried out a second round of PCR with primers targeting the P5 and P7 sequences only ...
-
bioRxiv - Genomics 2021Quote: ... samples were purified with 45 µl of Agencourt AMPure XP magnetic beads with a sample to beads ratio of 3:2 (Beckman Coulter), normalized by Qubit quantification and pooled to generate a 4 nM library ...
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested after 3 days and counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA).
-
bioRxiv - Cancer Biology 2024Quote: ... The percentage of fluorescence-positive cells was determined initially (baseline reference, passage 0, 3 days after transduction) and all following passages using the CytoFLEX Flow Cytometer (Beckman Coulter). In each passage ...
-
bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
-
bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
-
bioRxiv - Molecular Biology 2024Quote: ... bottom layer was made by diluting OptiPrep™ solution with clarified HM or digesta sample (2.25 mL OptiPrep™ + 0.75 mL sample = 3 mL) into a 5 mL open-top thin wall ultra-clear tube (Beckman Coulter). A discontinuous gradient was made with 40% (w/v) ...
-
bioRxiv - Bioengineering 2022Quote: ... Supernatant (33 mL) was placed on a 3 mL 25% (w/v) sucrose pad in SW28 tube (Beckman, Brea, CA, USA) and centrifuged at 24,000 rpm ...
-
bioRxiv - Cancer Biology 2023Quote: Counting of CB CD34+ cells after 3 days of ex vivo co-culture was accurately obtained with flow-count fluorosphere beads (Beckman Coulter), in combination with the following antibodies ...
-
bioRxiv - Microbiology 2024Quote: ... and resolved by ultracentrifugation at 36,000 rpm for 3 h at 4°C with the fastest braking in Optima XPN-80 Ultracentrifuge equipped with SW-41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 mL/fraction ...
-
bioRxiv - Immunology 2024Quote: ... Fluorescence signals and TruCount beads were acquired on a 3-laser CytoFLEX using Cytexpert software 2.3 (Beckman Coulter, Brea, CA, USA). Instrument quality control and standardization were performed daily using CytoFLEX Daily QC Fluorospheres (Beckman Coulter #B53230).
-
bioRxiv - Pathology 2024Quote: ... with primers located in the U3 region of the 5’ and 3’ LTRs (Figure S1) and purified using “1X AmpureXP beads” (Beckman Coulter). ONT (Oxford nanopore technologies ...
-
bioRxiv - Cancer Biology 2024Quote: ... washed 3 times with chilled phosphate-buffered saline (PBS) and analyzed by flow cytometry (CytoFLex, Beckman Coulter Inc., Brea, CA, USA)[39 ...
-
bioRxiv - Molecular Biology 2020Quote: ... MgCl2 5mM and Tris-Cl 50mM pH 7.4) was added into 3-mL polycarbonate tube for an ultracentrifuge TL100.3 rotor (Beckman Coulter, Brea, CA, USA). The KCl-adjusted ribosome-containing solution was carefully added above the sucrose cushion and tubes were balanced by weight using buffer B (sucrose 250mM ...
-
bioRxiv - Molecular Biology 2021Quote: ... Produced amplicons of 3-144 µm eukaryotes and Imitervirales were purified with the magnetic beads (Agencourt AMPure XP beads, Beckman Coulter, Inc.). Finial DNA production was dissolved in 27.5 µL ultrapure water ...
-
bioRxiv - Plant Biology 2021Quote: ... sucrose was transferred to the bottom of the tube using a Pasteur pipette and centrifuged at 27,000 rpm for 3 hours at 4° C using an SW28 Ti rotor (Beckman Coulter, Brea, CA). Each pellet containing the virus particles was resuspended in 1 mL resuspension buffer overnight with gentle shaking at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... supplemented with 100 μg/ml cycloheximide) and centrifuged for 3 hours at 35,000 rpm in an SW41 rotor in a Beckman L7 ultracentrifuge (Beckman Coulter, Krefeld, Germany). The gradients were simultaneously fractionated on a Density Gradient Fractionation System (#621140007 ...
-
bioRxiv - Biochemistry 2022Quote: ... Average fluorescence intensity of DCF from 3×105 cells was recorded and data acquisition and analysis were carried out using Summit 5.2 software (Beckman-Coulter, Inc. USA). Morphology of algal cells was examined and photographed with a differential interference contrast microscopy (DIC ...
-
bioRxiv - Cell Biology 2022Quote: ... The gradient was formed by sequentially adding each 3 ml layer to a 14 × 89 mm ultra-clear centrifuge tube (Beckman Coulter, Inc.), starting with the 40% solution and ending with the 5% solution ...
-
bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 16000 ×g for 3 hours at 4 °C in an Avanti J-25 centrifuge (Beckman Coulter Life Sciences, USA) with a JA-25.50 fixed-angle aluminum rotor (Beckman Coulter Life Sciences ...
-
bioRxiv - Molecular Biology 2021Quote: ... sucrose gradients prepared with lysis buffer without Triton X100 and centrifuged at 32,000 rpm for 3 hrs at 4 °C in an SW41Ti rotor (Beckman Coulter, Cat:331362). The sucrose gradients were fractionated using BR-188 Density Gradient Fractionation System with 60% (m/v ...
-
bioRxiv - Zoology 2021Quote: ... Food concentration was maintained at 1500 μm3 mL−1 of phytoplankton cells at the outlet of the tank – ad libitum (Rico-Villa et al., 2009) – and controlled twice daily using an electronic particle counter (Multisizer 3, Beckman Coulter, USA) equipped with a 100 μm aperture tube ...
-
bioRxiv - Neuroscience 2022Quote: ... and a 200-μl aliquot of the final supernatant was added to 3 mL of scintillation fluid and the radioactivity was measured by a liquid scintillation counter (Beckman LS 6000SC). Blank values were obtained by performing the reaction in the absence of tissue homogenate and in the presence of boiled homogenate ...
-
bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
-
bioRxiv - Cell Biology 2024Quote: ... conditioned media were subjected to 10 min at 200g for 3 cycles (G146D centrifuge, Gelec®) and 20 min at 2000g (Allegra 64R Centrifuge, Beckman Coulter) to remove cellular debris and apoptotic bodies respectively ...
-
bioRxiv - Biochemistry 2024Quote: 3 L of over-expressed cells were harvested immediately by centrifugation (Beckman Coulter Avanti J-20 XPI centrifuge, Beckman JLA-8.100 rotor) at 6,000 x g for 10 min at 4 °C ...
-
bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
-
bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Ribosome Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Ribosome Buffer containing 100 μg/mL cycloheximide in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
-
bioRxiv - Biophysics 2022Quote: ... The fractions containing monomeric peptide were pooled and centrifuged at 386000 g for 3 h in an Optima TLX ultracentrifuge equipped with a TLA 120.1 rotor (Beckman Coulter, Atlanta, GA, USA). Orthophosphoric acid or sodium phosphate were added to a final concentration of 20 mM to adjust the pH to 2.8 or 7.4 ...
-
bioRxiv - Biochemistry 2021Quote: The absorbance spectra of recombinant DosS and DosT (3 µM) were determined at room temperature using quartz cuvettes with rubber stoppers in a DU800 spectrophotometer (Beckman Coulter, Fullerton, CA). The reduction of recombinant DosS was achieved via addition of sodium dithionite (DTH ...
-
bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
-
bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
-
bioRxiv - Microbiology 2024Quote: ... aliquots of the cultures were fixed in 70% ethanol to analyze cell mass using the Multisizer 3 Coulter counter (Beckman Coulter, Brea, CA). The remaining cultures were further incubated for 4 h with 0.3 mg/mL rifampicin and 0.01 mg/mL cephalexin to allow run-out replication of chromosomal DNA ...
-
bioRxiv - Microbiology 2024Quote: The virus solutions were centrifuged at 106800 × g for 3 hours at 4 °C using a SW32Ti rotor (Beckman Coulter, Fullerton, CA, USA). The virus pellets were suspended in phosphate-buffered saline (PBS) ...
-
bioRxiv - Biochemistry 2023Quote: ... Colorimetric changes was measured 3 min after mixing the conditioned media with DTNB solution at 450 nm of absorbance (DTX880, Beckman Coulter, Indianapolis, IN, USA). The value from DTNB only was substracted as noise ...
-
bioRxiv - Bioengineering 2024Quote: ... using C4F10-saturated PBS until the subnatant was clear after which MBs were counted using a Coulter Counter Multisizer 3 (50 µm aperture tube, Beckman Coulter, Mijdrecht, the Netherlands). Next ...