Labshake search
Citations for Beckman :
1851 - 1900 of 1906 citations for 6 Bromo 3 N ethylamino 1 2 4 triazolo 4 3 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 0.1 ng cDNA was used per sample and the libraries were cleaned up with 1.8x AMPure® XP beads (Beckman Coulter). Quality and integrity of nucleic acids were assessed using the Agilent Technologies 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... either 150 μl or a maximum of 1 × 106 viable lymphocytes were analyzed using a CytoFLEX LX Flow Cytometer (Beckman Coulter), and data was analyzed with FlowJo software (FlowJo LCC ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were examined on either 3% agarose gel by electrophoresis at 130 V for 1 hour or using capillary electrophoresis on an automated CEQ2000 system (Beckman-Coulter) as described previously (Raman et al. ...
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bioRxiv - Biochemistry 2022Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and permeabilized in PBS/1% BSA/0.1% saponin before staining with a FITC-conjugated anti-Cap24 (KC57-FITC, Beckman Coulter, France) for 1h at room temperature ...
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bioRxiv - Synthetic Biology 2023Quote: ... Droplets were made by either manually pipetting 0.05-0.1 μL droplets or dispensing individual 2.5 nL - 20 nL droplets using an Echo 550 acoustic liquid handler (Beckman Coulter, USA) into 2 μL of the lipid-oil phase in 384-well microplate ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl or 10 μL of dilution were added to the 384-well plates using automated dispenser BioMek FX (Beckman Coulter). Extracellular bacteria were then eliminated by adding antibiotic to the media 1h after infection ...
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bioRxiv - Biochemistry 2023Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: GFP fluorescence level (FITC channel, signal amplification = 1) and cell size (FSC channel, signal amplification = 200) were measured with flow cytometer CytoFlex S (Beckman Counter) and CytExpert Software in 10000 cells (per each sample ...
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bioRxiv - Genomics 2023Quote: PCR reaction products were cleaned and size selected using a 0.7:1 ratio of AMPure XP SPRI beads (Beckman Coulter Inc.) to sample ...
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bioRxiv - Plant Biology 2024Quote: ... Then the reaction was started by adding 150 µL of a 1 M proline solution and the decrease in the OD600 monitored until at least 1 min of linear reaction was observed (DU-800 Spectrophotometer, Beckman Coulter). Reaction rates were calculated using the 600 nm absorbance coefficient of DCPIP (19.1 mM-1 cm-1 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 1% FBS in PBS antibody staining buffer and stained with the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 μl of the PCR reaction (roughly corresponding to 1 μg of amplicon) was then purified using a double-sided selection with AMpure XP beads (Beckman Coulter). First ...
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor, 288000 g). The excess monomeric protein and some small oligomers were then removed by multiple filtration steps using 100-kDa cut-off membranes ...
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987). Data were collected on Accuri C6 (BD Biosciences – U Mass ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting libraries were pooled and treated with AMPure XP magnetic beads at a ratio of 0.8:1 beads:sample volume to purify DNA (Beckman Coulter, Brea, CA, USA). Library yield and quality was determined by TapeStation electrophoresis with HSD1000 ScreenTapes (Agilent Technologies).
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bioRxiv - Immunology 2019Quote: ... SSM cultures were grown for ∼22 h at 36°C with shaking at 180 rpm until the OD600 reached 0.5 on a 1 cm path width spectrophotometer (Beckman Coulter DU 530). The cultures were then diluted to 1 × 109/ml with SSM.
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bioRxiv - Genomics 2019Quote: ... Cells were suspended with 300 μl 1% FBS /HBSS (with 10 mM sodium butyrate) and subjected to FACS (Beckman Coulter MoFlo XDP) sorting for tdTomato positive cells ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was then centrifuged at 4° C in the SW32Ti rotor with a speed of 30,000 rpm for 1 h to pellet the membrane fraction using an Optima L-90K Ultracentrifuge (Beckman Instruments, Brea, CA). The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... anti-CD105 and anti-HLA-ABC antibodies (suppl. Table 1) and analysed by flow cytometry (Cytoflex; Software Cytexpert 2.3, Beckman-Coulter, Krefeld, Germany). Upon passage 3 ...
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bioRxiv - Plant Biology 2020Quote: ... Soil pH and EC were 8.3 and 12 dS m−1 that were measured by a pH meter (Beckman 45 Modal, US) and EC meter (VWR Conductivity Meter DIG2052 ...
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bioRxiv - Microbiology 2019Quote: ... Permeabilized cells were then incubated with 100 μl of 1:100 dilution of phycoerythrin-conjugated mouse anti-p24 mAb (KC57-RD1; Beckman Coulter, Inc.) for 30 min at 4 °C with intermittent mixing ...
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bioRxiv - Microbiology 2020Quote: ... Membrane-containing fractions were collected in 1 mL after ultracentrifugation at 35,000 rpm on an SW-40 rotor (Beckman, Brea, CA USA) for 6 hours at 4°C and stored at -80°C prior to analysis.
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bioRxiv - Evolutionary Biology 2021Quote: ... with NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1) and AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). DNA content and quality of indexed libraries were examined with a Qubit 4.0 using the high-sensitivity kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... in order to achieve a starting density of 1.70 g mL-1 in a 3.3 mL polyallomer OptiSeal tubes (Beckman Coulter, Brea, CA, USA). After ultracentrifugation ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume:library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were permeabilized for intracellular staining via resuspension in 0.5% Triton X-100 in PBS for 10 min and were subsequently resuspended in a 1:500 dilution of anti-p24-FITC KC57 antibody (Beckman Coulter #6604665) in 1% BSA in PBS for 1 h at room temperature in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The percentage of live/dead cells was determined within 1 hour of staining by flow cytometry (CytoFLEX, Beckman Coulter, Indianapolis, IN, USA). 10,000 events were collected per sample ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were loaded onto a 1 M sucrose cushion and then pelleted by ultracentrifugation by using a TLA100.3 rotor (Beckman Coulter, cat#349490) for 1 hour at 100,000 rpm at 4°C ...
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bioRxiv - Physiology 2023Quote: ... via centrifugation in a SW41 rotor at 42,000 g at 4 °C for 1 hour in a Beckman Coulter Optima L-100 XP ultracentrifuge using 14 × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Mitochondria were harvested at the interphase between 19 and 40% Percoll ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells mixed with polybrene and lentivirus were then plated in 1 mL aliquots onto 12 well plates and centrifuged at 2000 rpm (931 x g) [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A] for 2 hours at 30°C ...
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bioRxiv - Microbiology 2024Quote: ... Liposomes and liposome-protein complexes were separated from unbound protein by ultracentrifugation of the reaction mixture in 5 mL Optiprep™ (Axis-Shield PoC AS) density gradients for 1 hour at 40,000 ×g (SW55Ti Beckman Coulter rotor). The liposome-protein mixture was adjusted to 34% Optiprep concentration in a volume of 300 μl ...
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bioRxiv - Pathology 2019Quote: ... PCR products were verified using gel electrophoresis (1% agarose gel) and cleaned with AMPure XP beads (Agencourt, Beckman Coulter Inc., Pasadena, CA, USA). Amplicons were then indexed using the Nextera XT Index Kit V2 set A and set D (Illumina ...
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bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
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bioRxiv - Biochemistry 2020Quote: ... The labeled and unlabeled polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, Beckman TLA-55 rotor). The pellets were rinsed once with 1 mL G-buffer and re-suspended into ice cold 0.5 mL G-buffer ...
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bioRxiv - Microbiology 2020Quote: ... samples absorbance at 485 nm was measured in a 1 cm quartz cuvette using a DUTM 730 spectrophotometer (Beckman Coulter, Brea, CA, USA). 155 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
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bioRxiv - Developmental Biology 2019Quote: ... washed, and fixed with 1% PFA (EM Sciences, Ft. Washington, PA) before analysis on a CyAn ADP Flow Cytometer (Beckman Coulter, Miami, FL). Data were collected on using a four-decade log amplifier and were stored in list mode for subsequent analysis using FlowJo Software.
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bioRxiv - Immunology 2019Quote: 100 ul of cell suspension for each of the dilutions including undiluted (1:0) was mixed with 400 ul of 1X PBS in the ViCell cuvette (Beckman Coulter, Part # 723908). Cell counting was performed based on manufacturer’s instructions (Beckman Coulter Vi-cell XR – 490992 ...
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bioRxiv - Biochemistry 2022Quote: ... The polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, TLA-55 rotor, Beckman Coulter, Indianapolis, IN). The pellets were rinsed once with a 1 mL G-buffer and re-suspended into ice-cold 0.5 mL G-buffer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequencing libraries for each replicate were generated via qPCR and sequencing libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume: library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent) ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 M sucrose in HK was added to the reaction to a final concentration of 1 M (“Input” sample) and transferred to 7 × 20 mm polycarbonate centrifuge tubes (Beckman Coulter cat #343775). HK buffer with 0.75 M sucrose was gently laid on top of the reaction mixture followed by a layer of HK ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
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bioRxiv - Molecular Biology 2021Quote: The VHH-coding regions within parent libraries and 1-round target-enriched sublibraries were PCR amplified and purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK). Then ...
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bioRxiv - Microbiology 2022Quote: ... Reaction products were verified on a 1% agarose gel and purified using the AMPure XP magnetic Bead protocol (Beckman Coulter Inc., Brea, CA, USA). PCR2 was performed in a 25 μl reaction ...
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bioRxiv - Bioengineering 2023Quote: ... and a humidity of 85 % in 48-well Flowerplates containing 800 µL defined CGXII medium with 0.5 % (w/v) glucose inoculated to an OD600 of 1 by using the BioLector microbioreactor for 24 h (Beckman Coulter Life Sciences, Aachen, Germany). Increasing concentrations of ANT (final concentration of 0 ...