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Citations for Beckman :
1701 - 1750 of 1769 citations for T cell leukemia lymphoma protein 1B TCL1B Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Cancer Biology 2022Quote: ... The rate of cell apoptosis was performed by Cyto-FLEX S Flow Cytometer and was analyzed using FlowJo 7.6 software (Beckman Coulter, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured for the indicated number of days before trypsinization and counting on a ViCell™-XR Cell Viability Analyzer (Beckman Coulter). Fold-change in cell number was calculated relative to a baseline cell count obtained by counting cells from replicate plates that were collected on the day of media exchange ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell pellets were rinsed twice with PBS and resuspended in PBS containing 0.1% BSA for fluorescence-activated cell sorting (FACS) (MoFlo Astrios EQs, Beckman Coulter, CA, USA). Before LC isolation ...
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bioRxiv - Molecular Biology 2022Quote: ... The percentage of live/dead cells was determined within 1 hour of staining by flow cytometry (CytoFLEX, Beckman Coulter, Indianapolis, IN, USA). 10,000 events were collected per sample ...
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bioRxiv - Biochemistry 2023Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Immunology 2023Quote: ... cells were activated with plate-bound anti-CD3 (500 ng/one well of 24-well culture plate; Beckman Coulter, REF# IM-1304) and soluble anti-CD28 (500 ng/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were harvested at 7 400 x g for 30 min in a JLA 9.1000 rotor (Beckman Coulter, Brea, California, USA), washed with 150 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Cells were collected at OD600 1.2 by centrifugation at 8000 rpm for 10 minutes (Beckman Coulter Avanti J-15R, rotor JA-10.100). Cell pellets were resuspended in 200 µl gradient buffer containing 20 mM Tris (pH 7.4 at 4°C) ...
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bioRxiv - Molecular Biology 2024Quote: ... The inhibitors were transferred to the designated wells in 384-well plates with 80% confluent cells using a ECHO525 Acoustic Liquid Handler (Beckman # 001-10080) to a final concentration of 1μM ...
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bioRxiv - Biochemistry 2024Quote: 3 L of over-expressed cells were harvested immediately by centrifugation (Beckman Coulter Avanti J-20 XPI centrifuge, Beckman JLA-8.100 rotor) at 6,000 x g for 10 min at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The cell lysate was centrifuged at about 16000 g for 10 min at 5 °C (Avanti J-265 XP, Beckman Coulter, USA). The starch pellet was recovered ...
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bioRxiv - Immunology 2024Quote: Stained cells were analyzed by a CytoFLEX Flow Cytometer with data being captured and processed using the CytExpert Software (Beckman Coulter, Inc). Subsequent analysis of the collected data was performed using FACS Express 6 software (De Novo Software).
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bioRxiv - Genomics 2024Quote: Cells from frozen stocks were diluted 1:30 into 300 µl of LB in a deep 96-well plate (Beckman Coulter, #267007), covered with a breathable film ...
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bioRxiv - Developmental Biology 2024Quote: ... 7 mL 1.4 g/mL CsCl and 22 mL cell lysate were layered from bottom to top in 38.5 mL tubes (Beckman Coulter, Brea, CA) and centrifuged at 110,880 x g for 1 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Cancer Biology 2020Quote: Cells were cultured in EV depleted medium (obtained by overnight ultracentrifugation at 100,000g, using a Beckman, XL-70 centrifuge with a 70Ti rotor) for 24h before supernatant collection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The percentage of cells within a defined range of fluorescence intensity was determined with Beckman Coulter CyAn ADP flow cytometer (Beckman Coulter, United States).
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bioRxiv - Genomics 2021Quote: ... The nuclear sorting was performed at the SciLifeLab Microbial Single Cell Genomics Facility with a MoFlo™ Astrios EQ sorter (Beckman Coulter, USA), as in Montoliu-Nerin et al. ...
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bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Biochemistry 2020Quote: ... was then added to the product which passed microfluidizer and unbroken cells and large debris were eliminated by centrifugation at 4000 × g (Beckman Coulter™, USA). The supernatant containing the IMVs was collected and washed several times with resuspension buffer by centrifugation at approximately 310,000 × g at 4°C for 30 minutes (SORVALL® Centrifuges ...
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bioRxiv - Cell Biology 2021Quote: ... Tert+ acinar cells were sorted into Tert+ and Tertcells among live cells based on the gate and collected by FACS (MoFlo®Astrios™, Beckman Coulter). C57BL/6J mice were used as negative controls for gating ...
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bioRxiv - Bioengineering 2020Quote: ... were generated using high-speed fluorescence-based cell sorting using various staining and gating strategies as described in the text (MoFlo Astrios, Beckman Coulter, Mississauga, ON).
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bioRxiv - Cell Biology 2021Quote: ... Supernatant was discarded and cells were resuspended in 100 μL FACs buffer and sorted with a CyAn™ ADP Analyser (Beckman Coulter, Inc.) at the UCL GOSICH Flow Cytometry Core Facility ...
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bioRxiv - Biophysics 2022Quote: ... ILC2s were sorted as PI-Lin-CD45+CD127+CRTH2+CD161+ cells via flow cytometry using MoFlo™ XDP (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
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bioRxiv - Immunology 2021Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were spun down at 4°C for 15 minutes at 4000 RPM (3040 RCF) in a J6 centrifuge (Beckman Coulter Life Sciences). Supernatant was discarded and pellets were stored in 15ml conical tubes in -80°C ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 Tl rotor (Beckman ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from fresh frozen tissue or cell lines using the Agencourt DNAdvance Beckman Coulter kit (#A48705, Beckman Coulter, Brea, CA, USA), fragmented and submitted to a series of enzymatic steps including End-repair and Adenylation ...
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bioRxiv - Neuroscience 2020Quote: ... 6ml of the cell suspension was carefully pipetted onto the top of the density gradient and centrifuged at 800g (Beckman, Cs-16R Centrifuge) for 30min at 22°C (with acceleration and deceleration rates set at 0) ...
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bioRxiv - Cell Biology 2021Quote: ... The fluorescence generated by the reduction of resazurin to resorufin by live cells was measured using a Beckman coulter DTX 880 multimode plate reader (Beckman Coulter, Brea, California) (570/600 nm).
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bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation at 6,000 ×g at 4 °C for 15 min with ALLEGRA X-30R (Beckman Coulter, Brea, CA, USA). The supernatant was centrifuged at 15,000 ×g at 4 °C for 15 min with Sorvall LYNX4000 (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: Cell growth was determined by measuring the absorbance of the culture at A600 (OD600) using a spectrophotometer (DU730, Beckman Coulter, Brea, CA, USA). The concentrations of the products (acetate and ethanol ...
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bioRxiv - Microbiology 2024Quote: ... Purified PCR products were measured and pooled for sequencing on an Illumina NextSeq 550 High Output Flow Cell using the single-end 75 base technique using AMPure XP SPRI beads (Beckman Coulter Life Sciences).
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bioRxiv - Evolutionary Biology 2023Quote: ... Five thousand cells from each of the 300 samples were analyzed in 96-well plates in a CytoFLEX Platform flow cytometer (Beckman Coulter, California, USA) at the Feldan Therapeutics facility (Quebec ...
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bioRxiv - Cell Biology 2023Quote: ... The stained cells were resuspended in PBS and analyzed using CytoFLEX S or Galios® flow cytometers (Beckman-Coulter Corp., Hialeah, FL, USA). Data analyses were performed using FlowJo version 10.
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 7h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 16h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000). The RING Zn-finger domains from both RING1B and PCGF4 subunits were cloned into a modified polycistronic expression vector (pET29a ...
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bioRxiv - Cancer Biology 2023Quote: ... Gating strategies were applied accordingly to remove any cell doublets and fluorescent signal was analysed using Kaluza software (Beckman Coulter Life Sciences, U.S.) (Supplementary Figure 1).
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bioRxiv - Microbiology 2022Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
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bioRxiv - Plant Biology 2023Quote: ... The disrupted cells were subjected to ultracentrifugation at 4°C and 235000 g for 1h (Beckman Optima™ L-80 XP, rotor Ti45). The resulting supernatant was loaded onto an Äkta purifier system (GE HealthCare ...
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bioRxiv - Cancer Biology 2024Quote: ... ATP production was detected with a CellCounting-Lite® 2.0 Luminescent Cell Viability Assay kit (Vazyme Biotech, Nanjing, China) by a plate reader (Beckman Coulter, Krefeld, Germany) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2024Quote: ... under a fluorescent dissecting scope and the remaining roots were subjected to 1.5 hours of protoplasting55. Each of the cell types with positive GFP/mCherry expression (Fig. S2) were collected by FACS (Beckman Coulter MoFlo XDP) in 15 mL tubes (Falcon ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of the cultures were fixed in 70% ethanol to analyze cell mass using the Multisizer 3 Coulter counter (Beckman Coulter, Brea, CA). The remaining cultures were further incubated for 4 h with 0.3 mg/mL rifampicin and 0.01 mg/mL cephalexin to allow run-out replication of chromosomal DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was discarded and the cell pellet resuspended in 500 µL 1x PBS + 1% BSA containing anti-BrdU (Beckman Dickson; 1:300) for 1 hr RT (cells were placed on a rotor and protected from light) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The red blood cell count of birds (number of red blood cells per ml of blood) was quantified immediately before the blood meal using flow cytometry (Beckman Coulter Counter, Series Z1). One hour after the last injection ...