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Citations for Beckman :
1601 - 1650 of 1703 citations for 2H Pyrazolo 4 3 c pyridine 3 chloro 4 5 6 7 tetrahydro since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Peptidoglycan was obtained by centrifuging for 13 min at 60,000 rpm at 20 °C (TLA100.3 Beckman rotor; OptimaTM Max ultracentrifuge Beckman, Beckman Coulter, California, USA). Pellets were washed 3-4 times by repeated cycles of centrifugation and resuspension in water ...
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bioRxiv - Genetics 2024Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean Ampure XP beads (Beckman Coulter, A63987).
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An anti-amyloidogenic treatment to specifically block the consolidation of traumatic events in mousebioRxiv - Animal Behavior and Cognition 2020Quote: ... the rest of the volume was centrifuged for 5 minutes at 4,000 rpm (Microfugue 18 centrifuge, Beckman Coulter) to obtain the cell debris fraction (P1 ...
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bioRxiv - Biochemistry 2020Quote: ... After 20 minutes the cells were collected by centrifugation (8,000 rpm, 5 minutes, JLA-16.25 Beckman Coulter rotor), dissolved in 0.5 mL of HEPES:Polymix buffer (5 mM Mg(OAc)2 ...
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bioRxiv - Genetics 2021Quote: ... Reactions were then pooled in a 5 ml tube and purified using a 0.7x SPRIselect (Beckman Coulter B23318) cleanup and eluted in 450μL of TE ...
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bioRxiv - Molecular Biology 2023Quote: ... Dialyzed nucleosomes were purified in a 5-30% w/v sucrose gradient with a SW41 Ti (Beckman Coulter) rotor in an Optima L-90K ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... 600 µL of the lysate were transferred to an ultracentrifuge tube (5 x 41 mm, Beckman-Coulter, #344090) and centrifuged in an MLS-50 rotor (Beckman-Coulter ...
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bioRxiv - Genetics 2024Quote: ... followed by inactivation at 85° for 5 min and cDNA purification with AMPure XP beads (Beckman Coulter, A63880) at a 1:1.1 sample-to-bead ratio ...
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bioRxiv - Biophysics 2021Quote: ... Cells were induced with IPTG and grown overnight at 37 °C and harvested by centrifugation in a Beckman Avanti J25 centrifuge with a JA-20 rotor at 5000 rpm (Beckman Coulter, Fullerton, CA). The cell pellet was resuspended in 10 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... 150 μl of the sample was ultracentrifuged at 22°C and 205000 × g for 19 min (TLA 100.3, Beckman Coulter Inc., Fullerton, California). The experiments were performed in HEPES buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The carboy was placed in an incubator (Sanyo MCO-17A1C) at 37 C and the optical density (OD) was monitored at 550 nm (Beckman DU-800 spectrophotometer) via 1 mL samples removed from the same port ...
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bioRxiv - Biophysics 2020Quote: ... Cells were grown in LB medium containing 50 μg/mL Kanamycin at 37°C to an OD600~1.0 (as measured in a Beckman Coulter DU 640 spectrophotometer) and then quickly transferred on ice to cool for ~20 mins ...
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bioRxiv - Cell Biology 2020Quote: ... followed by serial centrifugation at 100’000 g for 120 min at 10 °C (Beckman fixed-angle TL110 rotor; Beckman Optima Max-TL ultracentrifuge). The so obtained P100 pellets ...
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bioRxiv - Physiology 2023Quote: ... blood samples were centrifuged at 3000 RPM for 15 minutes at four °C in a refrigerated centrifuge (Beckman Coulter Allegra X-22 Series). Plasma samples were aliquoted and stored at -80°C ...
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bioRxiv - Microbiology 2024Quote: ... The lysate was incubated on ice for 10 minutes then spun down at 50,000 rpm at 10°C for 45 minutes (Beckman Coulter, 50.2 TI rotor). The supernatant was collected and treated with polyethyleneimine to a final concentration of 0.03% incubated on ice for 5 minutes and subsequently centrifuged at 10,000 rpm at 4°C for 10 minutes (Beckman coulter ...
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bioRxiv - Biophysics 2024Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour with a Beckman OptimaTM L-80XP Ultracentrifuge equipped with a Ti45 rotor (Beckman, Brea, CA, USA). The pellet containing membranes and cell debris was discarded ...
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bioRxiv - Immunology 2020Quote: ... and anti-CD27-PE-Cy5 (clone 1A4CD27; 2.5 μL; cat. # 6607107) and anti-NKG2A-APC (clone Z199; 5 μL; cat. # A60797) from Beckman Coulter (Supplementary Fig ...
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bioRxiv - Biochemistry 2020Quote: ... The cultures were grown until OD600 of 0.8 at 37 °C and the cells were collected by centrifugation at 8,000 rpm for 5 minutes in JLA-16.25 rotor (Beckman Coulter), dissolved in 0.5 mL of HEPES:Polymix buffer ...
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bioRxiv - Microbiology 2021Quote: ... liquid-captured bioaerosols (5 – 10 mL) were centrifuged for 10 minutes at 3000 × g (Allegra X-15R, Beckman Coulter) and resuspended in 200 μL of Middlebrook 7H9 medium supplemented with 100 μM DMN-tre ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 % solutions on top of each other in a 13.2 ml open-top polyallomer tube (331372, Beckman Coulter). The 1 ml concentrates were overlaid on top of the gradient ...
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bioRxiv - Microbiology 2021Quote: ... Linear sucrose gradients were prepared by layering 1.8 ml each of sucrose solutions (wt/wt; prepared in 5 mM EDTA, pH 7.5) into 14-by 95-mm ultracentrifuge tubes (Beckman) in the following order ...
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bioRxiv - Cancer Biology 2020Quote: ... equivalent amounts of ribosomes for each lysate was loaded onto each 10%-45% sucrose density gradient (BioComp Gradient Master machine) for ultracentrifugation at 33,000 rpm for 5 hours (Beckman Optima L80K Ultracentrifuge ...
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bioRxiv - Genetics 2020Quote: ... 5 μL of each PCR reaction from the single worm lysate was pooled and purified by SPRI (Beckman Coutler) to remove unincorporated oligos ...
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bioRxiv - Microbiology 2020Quote: ... The sample was vortexed for 10 s and incubated at room temperature for 5 minutes before centrifugation at 12,000 rpm (JA-15 1.5 rotor, Beckman Coulter) 4°C for 5 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed by centrifugation at 1,300 g for 5 min (Beckman Centrifuge Avanti® J-26-XP). Afterwards ...
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bioRxiv - Cell Biology 2022Quote: ... The stock solution of aggregate-depleted BSA (5% w/v) was prepared by overnight centrifugation at 100,000 x g (SW28 rotor Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... and 3.5 mL of 5% iodixanol in a 16.8 mL open top polyallomer tube (Beckman Coulter, Fullerton, California, USA). One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... respectively) were gently layered on top of each other in ultracentrifuge tubes (Ultra-Clear 5 mL Tube, Beckman Coulter) and a continuous gradient was generated using a gradient maker (Gradient Master ...
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bioRxiv - Microbiology 2024Quote: ... The phage was purified by isopycnic centrifugation in a CsCl discontinuous gradient for 5 h at 32,000 rpm in a SW41 rotor (Beckman) at 20°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Percentage of cells with each DNA content (2N, 3N, 4N, 5-8N) were estimated using CytExpert software (Beckman Coulter). For DNA content analysis using sorted cells ...
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bioRxiv - Cell Biology 2022Quote: ... Resuspended pellets were then subjected to an iodixanol density gradient in 5-mL Ultra-Clear centrifuge tubes (Beckman Coulter). The tubes were first filled with a two-layer gradient ...
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bioRxiv - Microbiology 2022Quote: ... the stained cells were detached by 5 mM of EDTA/PBS and analyzed with a CytoFLEX Flow Cytometer (Beckman). 10,000 events in a gated live cell population (based on SSC/FSC ...
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bioRxiv - Neuroscience 2024Quote: ... but 2.5 mL for 5%) and ultracentrifuged at 100,000 x g (SW 40 Ti or SW41 Ti rotor, Beckman Coulter) for 16 h using ultra-clear tubes (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were subjected to two rounds of centrifugation at 18000 rpm for 5 and 30 mins in a JA-25.50 rotor (Beckman). Protein levels were normalized for coimmunoprecipitation and immunoblot analysis using a Bradford Assay ...
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bioRxiv - Cell Biology 2024Quote: ... 10% and 0% in buffered sucrose on top of one another in 5 ml Ultra-Clear centrifuge tubes (Beckman). Gradients were centrifuged for 16 h at 100,000 x g and 4 °C ...
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bioRxiv - Genomics 2024Quote: ... Cell concentration was measured daily (particle size 5-8 um) using a Coulter counter (Coulter Z2, Beckman Coulter GmbH), in all experimental tanks ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and supernatants were ultracentrifuged at 37 000 rpm (~100 000 g) and 10°C for 1h (Beckman Optima LE-80 K; rotor 70.1.Ti). Pellets were resuspended in 100μL of buffer (10 mM Tris-HCL ...
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bioRxiv - Microbiology 2021Quote: ... The pellet was resuspended in pre-warmed ddH2O (about 60 °C) and centrifuged at least 10 times in an ultracentrifuge (Beckman Coulter, Pasadena, California, USA), until residual SDS was completely removed ...
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bioRxiv - Biophysics 2021Quote: ... Following incubation (37 °C, 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
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bioRxiv - Microbiology 2023Quote: ... and 1.45 g/mL layers and centrifuged for 1 h at 28,500 rpm at 15 °C using an Optima XE-90 Ultracentrifuge and SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter, Inc., Pasadena, CA, USA). After centrifugation ...
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bioRxiv - Genomics 2024Quote: ... cDNA release and collection is performed by incubating the chip in cDNA release mix overnight at 55*C and the release cDNA is purified with Ampure XP beads (Beckman Coulter; Cat. No. A63882) using the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2020Quote: ... Four mL of each density (3.5 mL for the 5% density) was stacked into an open polyallomer tube (337986, Beckman Coulter) using a Tecan freedom EVO ...
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bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5 × 105 additional cells were immediately processed for far red signal analysis by flow cytometry (Gallios, Beckman Coulter, Brea, USA) (D0) ...
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bioRxiv - Microbiology 2020Quote: ... the 96-well plate was centrifuged at 1,000 RPM for 5 min using an Allegra 25R model centrifuge (Beckman Coulter) with inserts for 96-well plates ...
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bioRxiv - Microbiology 2020Quote: ... At the end of each selection cells were pelleted from the media by centrifugation 200 x g for 5 min in an Allegra centrifuge (Beckman) and supernatant was transferred for storage at −80C ...
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bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
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bioRxiv - Immunology 2022Quote: ... 1.5 μl of PI was also added and samples were run on the Gallios™ Flow Cytometer (Beckman Coulter, Inc). Data was analysed offline using Kaluza® Flow Analysis software version 2.1a (Beckman Coulter Inc. ...
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bioRxiv - Immunology 2021Quote: ... subsequently overlaid onto 5 mL of 20% sucrose and ultracentrifuged at 24,000 rpm for 2.5 hrs (Beckman XPN80, SW32.Ti). The viral pellet was resuspended in PBS.