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Citations for Beckman :
1551 - 1600 of 1657 citations for 3 5 Diacetoxy 2 acetoxymethyl 6 phenethyl tetrahydro pyran 4 yl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Total RNA was isolated from Vero E6 and Caco-2 cells 48 hours after infection using RNAdvance Viral Kit (Beckman) using a KingFisher and RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were suspended in FACS buffer (PBS, 20 mM EDTA pH 8.0, 2% FCS) and acquired using a CyAn ADP9 flow cytometer (Beckman Coulter), FACS LSRII Fortessa or FACSymphony (both BD Biosciences) ...
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bioRxiv - Microbiology 2021Quote: ... pspAX.2 and oligonucleotides were incubated for 48 h and GFP expression level was analyzed by CytoFLEX flow cytometer (Beckman).
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bioRxiv - Neuroscience 2022Quote: ... new medium was added for another round of production and supernatant was filtered (0.45 μm filter) and centrifuged for 2 hours at 19400 rpm (SW28 Beckman rotor). The pellets were suspended in phosphate buffer saline (PBS) ...
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bioRxiv - Microbiology 2022Quote: ... was added before ultracentrifugation at 20,000 × g for 2 hours at 10°C in an Optima L-90K Ultracentrifuge with the SW32Ti rotor (Beckman Coulter). Supernatants were carefully discarded by aspiration and the 6 phage pellets were resuspended with 0.1 ml of TM buffer (50 mM Tris-HCl ...
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bioRxiv - Synthetic Biology 2021Quote: Reactions were assembled in a final volume of 2 μL using a Labcyte Echo® 550 (Beckman Coulter, Brea, CA) via a two-step strategy ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting homogenate was mixed with 6 mL sucrose (2 mol/L) and 2.5 mL CaCl2 (0.1 mmol/L) in an ultra-clear centrifuge tube (#344059, Beckman Coulter, USA). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were ultracentrifugated at 100,000g for 2 hours using OPTIMA MAX XP ULTRACENTRIFUGE with MLA-130 fixed-angle rotor (Beckman-Coulter) and pellets were resuspended in 0.22 μm-filtered PBS ...
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bioRxiv - Microbiology 2021Quote: ... 2 ml of each sucrose concentration was layered from 70% to 25% in open-top ultracentrifuge tubes (Beckman Coulter, 344059) immediately before use ...
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bioRxiv - Biochemistry 2021Quote: ... The depleted fraction was collected by gentle centrifugation (100 rcf for 2 min) into a clean 96 well plate (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... The top 11 mL and the bottom 2 mL of the gradient were transferred to separate 60 mL 45Ti ultracentrifuge tubes (Beckman), topped up with PBS and centrifuged at 100,000 x g for 70 min at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
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bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
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bioRxiv - Biophysics 2023Quote: ... the supernatant of cell lysates was centrifuged to isolate the total membrane fraction at 24,000 rpm for 2 h in the 45Ti rotor using ultracentrifuge (Beckman-Coulter). Membrane resuspension in 50 mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and spun at 100,000 x g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Neuroscience 2023Quote: ... This supernatant was next subjected to ultracentrifugation at 120,000 x g for 2 h at 4LJ in a SW 41 Ti Rotor Swinging-Bucket rotor (Beckman Coulter), and the pellets collected as small factors like exosome ...
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bioRxiv - Physiology 2023Quote: ... Samples were ultracentrifuged at 100,000 rpm at 16°C for 2 hours (rotor: Beckman TLA-100; Centrifuge: Beckman Optima TLX). 100ul from the top of the tube was collected as VLDL fraction ...
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bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
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bioRxiv - Immunology 2023Quote: The SARS-CoV-2 lentiviral pseudotyped neutralization assays were performed on integrated automation platforms consisting of a Biomek liquid handler from Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
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bioRxiv - Bioengineering 2022Quote: ... the conditioned media was subjected to an additional centrifugation step of 118,000 × g for 2 h in a Type 70 Ti ultracentrifuge rotor (Beckman Coulter). The resulting EV pellet was resuspended in 1X PBS and placed into a Nanosep 300 kDa MWCO spin column (Pall ...
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bioRxiv - Cell Biology 2022Quote: ... the virus-cell mixture was centrifuged at 1,000 rcf for 2 hours at 32°C (Beckman Coulter, Allegra X-30R). Infected cells were then cultured as described above and subsequently FACS-sorted for the establishment of clonal cell lines.
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bioRxiv - Microbiology 2023Quote: ... resuspended in 200 µl 2% PBS-BSA and analyzed for mean fluorescence intensity (MFI) of ACE2 expression with CytoFLEX flow cytometer (Beckman). To determine the biological effects of the purinergic receptor P2X7 and NLRP3 inflammasome on viral entry ...
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bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Fraction LP2 was obtained after centrifugation of fraction LS1 for 2 h at 165,000 g in a SW40 Ti rotor (Beckman, US). Corresponding supernatant was collected as Fraction LS2 ...
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bioRxiv - Immunology 2024Quote: ... and EVs were isolated by ultracentrifugation (UC) at 100,000 x g for 2 h using a SW40 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing the membrane fraction was transferred to Beckman ultracentrifuge tubes and subjected to ultracentrifugation for 2 h at 41,000 r.p.m (Beckman Optima L-100 XP ultracentrifuge, Beckman 45 Ti rotor). Membrane pellets were resuspended to a total protein concentration of 3.5 mg/mL in ice cold 1 x PBS using the Bicinchoninic Acid (BCA ...
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bioRxiv - Biochemistry 2024Quote: ... were added to the desired concentration before clarification by ultracentrifugation at 247,000 x (g) for 10 minutes at 2°C in a TLA100 (Beckman Coulter). Glucose oxidase (GmbH ...
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bioRxiv - Bioengineering 2024Quote: ... The standard GE-caveospheres and the disrupted GE-caveospheres were centrifuged at 120,000g for 2 hours in an TLA-55 Fixed-Angle Rotor of Optima MAX-XP Ultracentrifuge (Beckman coulter) followed by imaging of fluorescence using a ChemiDoc MP Imaging System as above ...
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bioRxiv - Biochemistry 2022Quote: ... Around 100 µL of sample at 15 µM was applied to each gradient and centrifuged by ultracentrifugation at 45 000 rpm at 4°C in SW 60 Ti Swinging-bucket rotor (Beckman Coulter, Palo Alto, US-CA) for 16 hours ...
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bioRxiv - Microbiology 2020Quote: ... Prepared phage suspension was transferred to U-formed centrifuge tubes suitable for ultra-centrifugation (25 000 × g, 120 min, 4 °C) (Beckman Coulter, Optima™ MAX-XP). Pellets were resuspended in 200 µL of SM buffer and phage stock suspensions were stored at 4°C and −80°C.
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bioRxiv - Cell Biology 2021Quote: ... This lysate was sonicated for 2 min (15 s on and 20 s off for 2 min at 20% amplitude) and centrifuged at 100,000 x g for 40 min at 4°C (Avanti J-301 Beckman Coulter centrifuge with rotor JA-30.50). The supernatant was filtered and passed through preequilibrated 5 ml HisTrap FF columns (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation at 8000 g at 4°C for 20 min (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 16.250).
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Bioengineering 2023Quote: ... subtilis spores were collected from the culture by the centrifugation at 8000 rpm at 4 °C for 20 min (Avanti J-E, Beckman Coulter Life Science, IN, USA). Supernatant was removed and the spore pellets were resuspended in fresh 100 mM PBS pH 7.4 ...
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bioRxiv - Biochemistry 2023Quote: ... lysosomes and mitochondria were enriched by centrifugation of the pooled PNS at 56,500 ×g for 7 min at 4 °C (Beckman-Coulter, 70 Ti fixed-angle rotor). The supernatant was removed ...
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bioRxiv - Biophysics 2022Quote: ... Labeled G-actin was clarified to remove any remaining F-actin or precipitated FMAL by centrifugation (10 min, 90,000 rpm in a Beckman TLA-120.2 rotor at 4°C). The extent of labeling (typically around 70% ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biophysics 2021Quote: ... Different ratios of LUVs and cyt c (with the protein concentration kept at 5 μM) were incubated in the sample buffer at room temperature for 15 min in ultracentrifugal tubes (Beckman Coulter, Indianapolis, IN). The effective PG/cyt c molar ratio (R ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected and centrifuged for 5 min at 1200 rpm at 20°C (Allegra™ X-12R Centrifuge, Beckman Coulter, the Netherlands). Cells were then resuspended in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were pelleted and washed twice before resuspension in 500μL of cold PBS for analysis using flow cytometry (CytoFLEX 5, Beckman Coulter, Mississauga, ON, Canada) wherein mean fluorescence intensity of the gated cells (10,000 events ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We pooled 1.5 µl of each indexed library (including blanks and museum controls) and performed size selection with AMPure XP beads (Beckman Coulter, IN, USA) for fragments of approximately 100-500 bp in length ...
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bioRxiv - Cell Biology 2024Quote: Lung nonparenchymal cells isolated from WT mice with and without LPS treatment (n=5/group) were stained as previously described (18) and analyzed using a CytoFlex (Beckman Coulter, Brea, CA). Dead cells were excluded using Live/Dead Near IR (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... thin-layer cell preparations for confocal microscopy of enriched small luteal cells and large luteal cells was performed using Cytofuge 2 (Beckman Counter). Cells were then fixed with 10% neutral buffered formalin at 4 °C for 30 min ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We combined the 8 wells from column 2 of each plate and used the pooled culture to measure cell density on a Coulter Counter Z2 (Beckman Coulter). We spotted the entire volume of each of the other 88 wells on CSM-Arg (Sunrise Scientific ...
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bioRxiv - Genomics 2020Quote: The ∼400 bp amplicon product produced using both Pool-1 and Pool-2 from the same clinical sample was combined and purified using a 1X concentration of Agencourt AMPure XP beads (Beckman Coulter); the final product was eluted in 30 µl of elution buffer (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... The donor-derived LT-HSC cell populations were sorted according to the gating strategy in Extended data Figure 2 using MoFlo Astrios Cell Sorter (Beckman Coulter). LT-HSC cells were directly sorted into 50μl RNA extraction buffer (ARCTURUS PicoPure RNA Isolation Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Washed pellets were resuspended in 2 mL 1x PBS and fluorescence was calculated using a LSR II cell analyzer (Beckman Coulter). Two biological replicates of 50,000 cell counts were analyzed with FlowJo ...
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bioRxiv - Molecular Biology 2022Quote: Procyclic form cell lines for depletion of either TbPH1 or TbKifX2A were induced at a density of 2×106 cells/ml with 1 µg/ml doxycycline and growth monitored daily with a Z2 Coulter Counter Analyzer (Beckman Coulter). Cells were diluted back to the initial cell density every day ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...