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Citations for Beckman :
101 - 150 of 272 citations for Small ribosomal subunit protein uS12 RPS23 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... and small-sized EVs were isolated in two steps by ultracentrifugation using a 70Ti rotor (Beckman Coulter, USA). First ...
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bioRxiv - Microbiology 2020Quote: ... Small gDNA molecules were removed by mixing sonicated gDNA with 0.6X Agencourt Ampure XL magnetic beads (Beckman Coulter) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then analyzed by flow cytometry using a CytoFLEX S machine with a small particle detector (Beckman Coulter), and the concentration of the SybrGold positive population defined as virus particles (VP ...
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bioRxiv - Microbiology 2020Quote: ... FITC−Dylight 405+APC+ Env-specific B cells were sorted using MoFloAstrios EQ (Beckman Coulter). For bulk sorting ...
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bioRxiv - Cancer Biology 2023Quote: ... the stage of apoptosis was assessed using the Annexin V FITC kit (Beckman Coulter, IM2375) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in staining solution containing anti-p24 FITC (Beckman Coulter #6604665; clone KC57) and BD Perm/Wash Buffer for a final staining volume of 50μl ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was washed with PBS and ultracentrifuged at 100,000 × g 1h at 4°C to obtain small vesicles (TL-100 Ultracentrifuge, Beckman Coulter). The final pellet was resuspended in PBS for subsequent experiments ...
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bioRxiv - Microbiology 2022Quote: ... with the following modification for FFPE material during size exclusion: small fragments were retained and purified twice with 1.2× Ampure XP Beads (Beckman Coulter). Libraries were quantified with the QIAseq Library Quant Assay Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... 1:20 human FITC-BRIC6 (IBGRL Research) and 1:20 human APC-anti-CD49d (Beckman Coulter). For viability analyses ...
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bioRxiv - Immunology 2024Quote: ... and stained for Gag p24 protein with anti-HIV-1 core antigen monoclonal antibody (clone KC57, Beckman Coulter, cat. 6604665).
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bioRxiv - Cell Biology 2020Quote: ... thin-layer cell preparations for confocal microscopy of enriched small luteal cells and large luteal cells was performed using Cytofuge 2 (Beckman Counter). Cells were then fixed with 10% neutral buffered formalin at 4 °C for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... in order to concentrate the medium to use the ultracentrifuge tubes with a reduced volume capacity for the small EV isolation (8.9 ml polypropylene centrifuge tube, Beckman Coulter, 361623). The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor ...
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bioRxiv - Cancer Biology 2022Quote: ... vehicle control or compounds from the small molecule library (Supplementary Table 4) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were then incubated in an IncuCyte Zoom (Sartorius ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA library was purified and size selected using a double-sided bead purification protocol with AMPure XP beads that removes both unwanted small and large fragments (Beckman Coulter). The QuBit dsDNA HS Assay (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and the fluorescent anti-p24 mAb (PE or FITC-conjugated anti-p24, clone KC57; Beckman Coulter/Immunotech). The percentage of infected cells (p24+ cells ...
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Immunology 2023Quote: ... labelled BM-MDSCs were stained with the Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter, IL, Italy) following manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... the fluorescence of Bodipy-C11 was collected from the FITC channel on a Gallios cytometer (Beckman-Coulter).
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bioRxiv - Immunology 2022Quote: ... and the expression of the Gag- p24 viral protein was detected with a PE-anti-p24 antibody (clone KC57 RD1; Beckman Coulter). Cell viability was determined using an aqua viability dye for flow cytometry (LIVE/DEAD® Fixable Aqua Dead Cell Stain kit ...
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bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged in a single 2 h run with sedimentation at 50,000 g and 4°C using small 13 x 51 mm ultracentrifugation tubes in SW55 Ti rotor (Beckman Coulter, Krefeld, Germany). These centrifugation settings were optimized for polysome and monosome separations ...
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bioRxiv - Microbiology 2022Quote: ... Assay-ready plates were generated at the UW-Madison Small Molecule Screening Facility with a Labcyte Echo 650 (Beckman Coulter, Brea, CA) liquid handler by spotting each compound onto 96-well assay plates (Greiner Bio-One ...
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bioRxiv - Immunology 2022Quote: ... Staining and bacterial count (events/μl in the FITC +ve gate) was assessed by flow cytometry (CytoFLEX, Beckman-Coulter). The pH responsiveness of CypHer5E was tested by measuring the bacterial fluorescent staining in the APC channel before and after the addition of 1 μl of sodium acetate (3 M ...
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bioRxiv - Microbiology 2021Quote: ... Gating in SSC‐A and GFP FITC‐A channels in the flow cytometry analysis software (Beckman Coulter Cytexpert 2.3.0.84) allows finding of (fluorescent ...
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bioRxiv - Immunology 2021Quote: ... using a 12-parameter panel (CD57-FITC, CD4-PE, CD161-PC7, CD8-KrO, CD279-PC5.5, CD45RA-A700, CD3-APC-A750 [Beckman Coulter] and CD56-BV605 ...
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bioRxiv - Immunology 2022Quote: ... human Vγ9Vδ2 T lymphocytes were collected and stained with a FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and analyzed by flow cytometry ...
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bioRxiv - Cell Biology 2022Quote: ... with known Equivalent number of Reference Fluorophores (ERF) values for C30/FITC/APC (as determined on a Beckman Coulter CytoFLEX) were used in the standardization of the fluorescent detection channels Ch01,Ch02 & Ch05 ...
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bioRxiv - Microbiology 2021Quote: The following fluorochrome-conjugated Abs were used for flow cytometry analysis: HIV-p24 FITC (KC57) (Beckman Coulter, Fullerton, CA, USA), HIV-p24 PE (KC57 ...
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bioRxiv - Immunology 2020Quote: ... PE-CD11b and FITC-CD3 (all Ebioscience) for 20 minutes and washed before sorting using a Moflo Astrios (Beckman Coulter) or FACSAria IIu (BD BioSciences) ...
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were fixed and permeabilized (NordicMUBio, Cat#GAS-002) and then stained intracellularly for Gag (FITC conjugated; 6604665; Beckman Coulter) and ZAP (GenTex ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of productively infected cells (p24+ ...
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bioRxiv - Cancer Biology 2020Quote: ... the pellets were resuspended in 500 μl PBS and analyzed by flow cytometry using the FITC channel of a FC-500 flow cytometer (Beckman Coulter). All manipulations were performed in 1.7 ml Eppendorf tubes ...
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bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... followed by flow cytometry analysis of CAp24 antigen expression on fixed and permeabilized cells labelled with KC57-fluorescein isothiocyanate (FITC) (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Cancer Biology 2022Quote: ... The cell pellet was disaggregated and incubated with 2 μL of anti-pan cytokeratin-FITC (Beckman Coulter, Cat. No. IM2356U, Clone J1B3). A separate tube containing cells unstained for surface E-Cadherin was incubated after permeabilization with E-Cadherin antibody- (R&D Cat ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... we used an AV-FITC/PI apoptosis detection kit to detect programmed cell death using flow cytometry (Cytoflex, Beckman Coulter, CA, USA), as previously described (49 ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
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bioRxiv - Microbiology 2024Quote: ... Apoptotic cells were stained using an Annexin V-FITC Apoptosis Detection Kit (Beyotime, Shanghai, China) and analyzed using a CytoFLEX Flow Cytometer (Beckman, Brea, USA).
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE or FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) Productively-infected cells were identified based on p24 and CD4 detection as described above ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE or FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of infected cells (p24+ ...
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bioRxiv - Molecular Biology 2023Quote: Proteins were collected by centrifugation (Beckman-Coulter JA-14 rotor ...
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bioRxiv - Immunology 2020Quote: ... and with Cyto-stat tetrachrome (FITC anti-CD45, PECy5 anti-CD3, PE anti-CD4, and ECD anti-CD8, Beckman Coulter, catalog number 660713). Unstimulated cells and pokeweed mitogen-stimulated cells were additionally stained with AF750 anti-CD19 (Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... 2G12 was washed out with PBS twice followed by permeabilization with 0.2% Triton X-100 and staining for intracellular Gag with KC57-FITC (Beckman Coulter, Pasadena, CA; 6604665) diluted 1:100 in Dako antibody diluent ...
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bioRxiv - Cell Biology 2020Quote: ... Protein aggregates were pelleted by ultracentrifugation of the proteins individually at 80,000 rpm in TLA 100 (Beckman Coulter) for 15 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Amnion-derived MSCs from placental tissue were isolated and characterized with CD73-APC, CD90-FITC and CD105-PE-Cy7 (all from eBioscience, San Diego, CA) by flow cytometry (CytoFLEX XL, Beckman Coulter GmbH, Krefeld, Germany) [21] and HUVECs were isolated from the umbilical cord and characterized as published previously [25].
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb or using PE of FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). Cell-surface staining of infected primary MDMs cells was performed five days post-infection ...
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bioRxiv - Microbiology 2020Quote: ... The proteins were sedimented in the SW40Ti rotor (Beckman Coulter) at 100,000 × g for 17 h at 4 °C ...