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Citations for Beckman :
101 - 150 of 539 citations for Probable U3 small nucleolar RNA associated protein 11 UTP11 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Purified cDNA was amplified for 11 cycles before being cleaned up using SPRIselect beads (Beckman Coulter). Samples were diluted 9:1 (elution buffer (Qiagen):cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were amplified for 11 PCR cycles and size selected using SPRI selected beads (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... FITC+ APC+ probe-specific B cells were sorted using MoFloAstrios EQ (Beckman Coulter). For bulk sorting ...
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bioRxiv - Microbiology 2020Quote: ... FITC+APC+ Env-specific B cells were sorted using MoFloAstrios EQ (Beckman Coulter). Rabbit PBMCs obtained 30 days after the last injection were prepared for sorting ...
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bioRxiv - Bioengineering 2023Quote: ... The GFP signal was detected by FITC channel using CytoFLEX Flow Cytometer (Beckman). 10,000 events were counted from each sample for FCM analysis ...
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bioRxiv - Genomics 2020Quote: ... Small molecules in the prepared libraries were removed by size selection using Agencourt AMPure XP (Beckman Coulter). The numbers of PCR cycles and conditions of size selection for individual libraries are included in Table 1 ...
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bioRxiv - Genomics 2020Quote: ... and cleaned with 0.7X AMPure beads to remove small fragments and excess reagents (Beckman Coulter, Brea, CA). The genomic DNA libraries were then captured following the SeqCap protocol which was modified to increase final capture reaction volume by 1.5X (Roche ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 20 min at 20 000 g to pellet mitochondria (8 °C, 11 300 RPM, Beckman JA-13.1), 40 min at 20 000 g (8 °C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and further centrifuged 20 min at 20 000 g (8 °C, 11 300 RPM, Beckman JA-13.1) to pellet mitochondria after Percoll removal by dilution with 40 mL mitochondrial wash buffer (250 mM Sorbitol ...
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bioRxiv - Pathology 2021Quote: ... 20 U/ml SUPERase·In) in thickwall 11 x 34 mm polycarbonate tubes (Beckman Coulter Life Sciences; 343778). Samples were weighed and balanced by adding PEB and centrifuged in TLA100.3 rotor at 100,000 rpm for 1.4 h in a pre-chilled ultracentrifuge (Beckman coulter Optima MAX-XP Ultracentrifuge) ...
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bioRxiv - Immunology 2024Quote: Antibodies and clones against the following proteins were used (clone, fluorophore, company): CD3 (UCTH1, PECy5, Beckman Coulter), CD7 (Horizon V450 or BUV395 ...
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bioRxiv - Microbiology 2021Quote: ... PE) and HIV-1 Gag core antigen (FH190-1-1, Beckman Coulter; PE, FITC). All samples were acquired on either an BD Fortessa X20 or LSR II using BD FACSDiva software and analyzed using FlowJo v10 (Tree Star) ...
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bioRxiv - Immunology 2023Quote: ... FITC-conjugated anti Vδ2 and PE-conjugated anti CD69 mAbs were purchased from Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... The cells were stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (Beckman Coulter 6604667 in BD Perm/Wash Buffer for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... while PE or FITC-conjugated Mouse anti-HIV-1 p24 (clone KC57; Beckman coulter) were used for intracellular staining.
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bioRxiv - Plant Biology 2020Quote: ... small fragments were removed with a size-selection step using 1x AMPure XP (Beckman Coulter, Brea, CA, USA) in NaCl/PEG buffer (10 mM Tris-HCl ...
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bioRxiv - Bioengineering 2021Quote: ... and small-sized EVs were isolated in two steps by ultracentrifugation using a 70Ti rotor (Beckman Coulter, USA). First ...
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bioRxiv - Microbiology 2020Quote: ... Small gDNA molecules were removed by mixing sonicated gDNA with 0.6X Agencourt Ampure XL magnetic beads (Beckman Coulter) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... We purified the folded chassis by glycerol gradient sedimentation by centrifugation[47] through a 10-45% glycerol gradient in TBE buffer supplemented with 11 mM MgCl2 for 130 min at 242,704g in a SW50.1 rotor (Beckman) at 4°C and collected in fractions ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then analyzed by flow cytometry using a CytoFLEX S machine with a small particle detector (Beckman Coulter), and the concentration of the SybrGold positive population defined as virus particles (VP ...
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bioRxiv - Microbiology 2020Quote: ... FITC−Dylight 405+APC+ Env-specific B cells were sorted using MoFloAstrios EQ (Beckman Coulter). For bulk sorting ...
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bioRxiv - Cancer Biology 2023Quote: ... the stage of apoptosis was assessed using the Annexin V FITC kit (Beckman Coulter, IM2375) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in staining solution containing anti-p24 FITC (Beckman Coulter #6604665; clone KC57) and BD Perm/Wash Buffer for a final staining volume of 50μl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Linear 10–30% glycerol gradients were prepared in 11□mm□×□34□mm polyallomer centrifuge tubes (Beckman Coulter, Brea, CA) with HEM buffer [25 mM HEPES (pH 7.5) ...
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bioRxiv - Cancer Biology 2021Quote: ... The pellet was washed with PBS and ultracentrifuged at 100,000 × g 1h at 4°C to obtain small vesicles (TL-100 Ultracentrifuge, Beckman Coulter). The final pellet was resuspended in PBS for subsequent experiments ...
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bioRxiv - Microbiology 2022Quote: ... with the following modification for FFPE material during size exclusion: small fragments were retained and purified twice with 1.2× Ampure XP Beads (Beckman Coulter). Libraries were quantified with the QIAseq Library Quant Assay Kit (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... 1:20 human FITC-BRIC6 (IBGRL Research) and 1:20 human APC-anti-CD49d (Beckman Coulter). For viability analyses ...
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bioRxiv - Cell Biology 2020Quote: ... and layered onto a continuous 10-50 w/w % sucrose gradient prepared in a 2.2 ml ultra-clear tube (11×34 mm, Beckman Coulter) using a two-step program in Gradient Master 108 machine ...
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bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
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bioRxiv - Microbiology 2020Quote: ... and the resulting cDNA was PCR amplified (11 cycles) and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). For Illumina NextSeq sequencing ...
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bioRxiv - Microbiology 2020Quote: ... pH 7.5) at 45,000 RPM for 45 minutes at 11 °C in a Beckman 70.1 Ti rotor (Beckman Coulter, Brea, California). The pellet was resuspended in 10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2023Quote: ... The top 11 mL and the bottom 2 mL of the gradient were transferred to separate 60 mL 45Ti ultracentrifuge tubes (Beckman), topped up with PBS and centrifuged at 100,000 x g for 70 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Sucrose step gradient (sucrose in resuspension buffer) were prepared in Ultra-Clear™ centrifuge tube 11×89 mm (Beckman Coulter) starting from the bottom (%w/w) ...
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bioRxiv - Immunology 2024Quote: ... and stained for Gag p24 protein with anti-HIV-1 core antigen monoclonal antibody (clone KC57, Beckman Coulter, cat. 6604665).
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bioRxiv - Cell Biology 2020Quote: ... thin-layer cell preparations for confocal microscopy of enriched small luteal cells and large luteal cells was performed using Cytofuge 2 (Beckman Counter). Cells were then fixed with 10% neutral buffered formalin at 4 °C for 30 min ...
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bioRxiv - Biophysics 2022Quote: ... in order to concentrate the medium to use the ultracentrifuge tubes with a reduced volume capacity for the small EV isolation (8.9 ml polypropylene centrifuge tube, Beckman Coulter, 361623). The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor ...
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bioRxiv - Cancer Biology 2022Quote: ... vehicle control or compounds from the small molecule library (Supplementary Table 4) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were then incubated in an IncuCyte Zoom (Sartorius ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA library was purified and size selected using a double-sided bead purification protocol with AMPure XP beads that removes both unwanted small and large fragments (Beckman Coulter). The QuBit dsDNA HS Assay (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... and the fluorescent anti-p24 mAb (PE or FITC-conjugated anti-p24, clone KC57; Beckman Coulter/Immunotech). The percentage of infected cells (p24+ cells ...
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Immunology 2023Quote: ... labelled BM-MDSCs were stained with the Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter, IL, Italy) following manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... the fluorescence of Bodipy-C11 was collected from the FITC channel on a Gallios cytometer (Beckman-Coulter).
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bioRxiv - Microbiology 2021Quote: ... starting with 50% and topping off with 15% for a total of 11 mL was added to thin wall polypropylene ultracentrifuge tubes (Beckman Coulter). The tubes were immediately frozen and stored at -80 °C ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Immunology 2022Quote: ... and the expression of the Gag- p24 viral protein was detected with a PE-anti-p24 antibody (clone KC57 RD1; Beckman Coulter). Cell viability was determined using an aqua viability dye for flow cytometry (LIVE/DEAD® Fixable Aqua Dead Cell Stain kit ...
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bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged in a single 2 h run with sedimentation at 50,000 g and 4°C using small 13 x 51 mm ultracentrifugation tubes in SW55 Ti rotor (Beckman Coulter, Krefeld, Germany). These centrifugation settings were optimized for polysome and monosome separations ...
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bioRxiv - Microbiology 2022Quote: ... Assay-ready plates were generated at the UW-Madison Small Molecule Screening Facility with a Labcyte Echo 650 (Beckman Coulter, Brea, CA) liquid handler by spotting each compound onto 96-well assay plates (Greiner Bio-One ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell suspensions were diluted 250 μL into 10 mL of ISOTON diluent and cell size was measured on a Coulter Z2 Cell Analyzer (Cat# 844 80 11, Beckman, Indianapolis, IN). Median cell volume was used for calculations ...