Labshake search
Citations for Beckman :
101 - 132 of 132 citations for Poloxamer 188 solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and fragments between 400-500 bp were selected for purification using a 25X AMPure beads solution (Beckman Coulter Inc, Indianapolis, IN). Fragments were then amplified with a NEBNext Ultra Q5 PCR protocol ...
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed with 25 mm supplemented HBSS before depleting the sample of unnucleated cells using IOTest 3 Lysing solution (Beckman Coulter) following provider instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Sample solution containing EVs was placed at the top of the tube (at RT) and ultracentrifugation (Beckman Optima L-90K Ultracentrifuge) was performed at 40,000 RPM for 16 hours at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... 750 µL of the 80% Histodenz solution were added and thoroughly mixed with 750µL of the protein-LUV sample in Thinwall centrifuge tubes (Beckman 50.2ti rotor). 1.5 mL of the 30% Histodenz solution was added as the intermediate layer ...
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bioRxiv - Cell Biology 2022Quote: ... which was then slowly poured on top of 10 ml cushion solution (2.05M sucrose with protease inhibitors, DTT and PMSF) in the ultracentrifugation tube (Beckman polyallomer #326823). After a 45-min spin at 24,600 rpm in pre-chilled SW32Ti rotor at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were brought to a final volume of 300 μl if necessary with lysis buffer and loaded on a non-linear sucrose gradient prepared by layering different percentage of sucrose solutions (5%, 34% and 55%) into an Open-Top Thinwall Ultra-Clear Tube (Beckman, 344059). A 5x gradient buffer (100 mM HEPES pH 7.6 ...
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bioRxiv - Microbiology 2024Quote: ... cultures were diluted 1:20 in filtered saline solution (NaCl 0.9%) and fluorescent intensity was measured by flow cytometry using a CytoFLEX-S cytometer (Beckman Coulter, USA). The measurement of each biological replicate is the result of the mean of the fluorescence intensity of 30 000 events per sample ...
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bioRxiv - Cancer Biology 2024Quote: ... Conventional flow cytometry analysis was performed on 200 µL of whole blood cells using a lyse no wash protocol (Versalyse lysing solution, Beckman Coulter) or on 2 ×106 PBMC labeled and analyzed using a Fortessa cytometer (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT and 10 mM Tris-HCl in DEPC-treated water was added to the bottom of the lysis solution in ultracentrifuge tubes (Beckman Coulter) to form a gradient ...
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bioRxiv - Plant Biology 2023Quote: ... Uptake was terminated by rapid filtering of the cells into the kill solution during 20 s centrifugation at set maximum speed (microfuge B, Beckman, USA). For dark-inactivation experiments ...
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bioRxiv - Cell Biology 2023Quote: ... protein-liposome or protein-LD mixtures were combined with equal volume of 60% Optiprep solution (D1556-250ML, sigma) and placed at the bottom of the centrifuge tube (Beckman, 344090), and 10% Opti-prep and 0% Optiprep solutions were carefully pipetted on the top ...
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bioRxiv - Evolutionary Biology 2024Quote: ... to 1.5 mM with a 10-fold dilution and applied double-sided size selection with 90 µL of Agencourt AMPure XP solution (Beckman Coulter) at both removing long fragments and recovering short fragments ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Genetics 2022Quote: ... The samples were size selected with 0.65x volume of Agencourt® AMPure® XP magnetic bead solution (Beckman Coulter, Brea, CA, USA), and suspended in 20µL elution buffer (Qiagen ...
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bioRxiv - Genetics 2022Quote: ... We cleaned the PCR product with 1.8x volume of Agencourt® AMPure® XP magnetic bead solution (Beckman Coulter, Brea, CA, USA), and suspended in 40µL elution buffer (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... The nuclei suspension was layered onto 10 mL of 29% OptiPrep solution (Buffer + 50% OptiPrep) in a 38.5 mL Ultra-Clear tube (Beckman-Coulter, CA, USA) before being centrifuged at 7,333g for 30 minutes at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Immunology 2023Quote: ... After adding 450 μL of RBC lysis solution (QuantoBio Technology, Beijing, China) and 15 min incubation the samples were analysed using a DxFLEX flow cytometer (Beckman Coulter, USA). All data were analysed using FlowJo X (FlowJo ...
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bioRxiv - Plant Biology 2023Quote: ... The combined cAW and vAW solutions were then centrifuged using 29 x 104 mm (50 mL) polypropylene centrifuge tubes (Beckman Coulter, # 357007) at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... saturated solution) to reach a final sucrose concentration of ∼40% and layered onto 62% sucrose in polycarbonate centrifuge tubes (Beckman Coulter, 34058). 35% (1.02M) ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting lysate was loaded on top of 10 ml 29% iso-osmolar OptiPrep solution in a conical 30 ml centrifuge tube (Beckman Coulter, SW28 rotor). Samples were centrifuged at 10,100 × g (7,500 r.p.m. ...
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bioRxiv - Microbiology 2024Quote: The virus solutions were centrifuged at 106800 × g for 3 hours at 4 °C using a SW32Ti rotor (Beckman Coulter, Fullerton, CA, USA). The virus pellets were suspended in phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2023Quote: ... 80 µl aliquots of the soil solution diluted in each medium were dispensed into the wells of 384-well plates using a Biomek liquid handler (Beckman Coulter, Indianapolis, IN, USA). Three plates per corrinoid condition were inoculated ...
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bioRxiv - Biochemistry 2023Quote: ... Colorimetric changes was measured 3 min after mixing the conditioned media with DTNB solution at 450 nm of absorbance (DTX880, Beckman Coulter, Indianapolis, IN, USA). The value from DTNB only was substracted as noise ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 ml of each solution were used to form the gradient in a 14 ml Ultra-Clear™ centrifuge tube (Beckman Coulter, Brea, CA, USA), with the highest sucrose concentration at the bottom of the tube ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were then added to the tubes and then solutions were allowed to stand for 20 min and the optical absorbance at 620nm was measured with DU800 spectrophotometer (Beckman Coulter Inc., Brea, CA, USA). The malachite green-ammonium molybdate solution was prepared as described by Onodera et ...