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Citations for Beckman :
101 - 150 of 702 citations for Human Immunodeficiency Virus GP41 Protein HIV 1 Group O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... The virally-transduced human CD34+ were sorted for GFP reporter expresssion on a MoFlo high-speed cell sorter (Beckman-Coulter) located at the New York Blood Center ...
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bioRxiv - Immunology 2021Quote: ... as assessed by flow cytometry with Korman orange-conjugated mouse anti-human CD19 monoclonal antibody (mAb) (Beckman Coulter, CA, USA). The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza ...
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bioRxiv - Immunology 2023Quote: Human TCR Vβ repertoire analysis was performed using antibodies against the most frequent TCR Vβ variants (covering about 70% normal human TCR Vβ repertoire, as reported by Beckman Coulter “Beta Mark TCR Vbeta Repertoire Kit” (cat.nr. ...
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bioRxiv - Cell Biology 2019Quote: ... The protein solution was then centrifuged at 100,000 rpm in an Airfuge (Beckman Coulter) to remove aggregates ...
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bioRxiv - Cell Biology 2019Quote: Viability of unlabeled and ferumoxytol-labeled murine MSCs (n=4) and human MSCs (n=3) was assessed by Trypan blue dye exclusion using ViCell (Beckman Coulter).
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bioRxiv - Cancer Biology 2019Quote: Cells were stained for 30 min (minutes) with the anti-human monoclonal antibody CD11b-PE (IM2581U, Beckman Coulter, Brea, CA, USA), CD14-FITC (555397 ...
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bioRxiv - Cancer Biology 2024Quote: Analysis of GALNT3 or fibronectin expression on human or mouse pancreatic cancer cells was performed using a flow cytometer (Gallios, Beckman Coulter) with appropriate antibodies ...
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bioRxiv - Immunology 2024Quote: ... The sections were washed and incubated for two hours with anti-human mouse CD14 Texas Red (clone: RMO52) (Beckman Coulter, USA). Afterward ...
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bioRxiv - Cell Biology 2021Quote: ... Monoclonal antibodies recognizing the closed conformation of β1 integrin (unoccupied β1 integrin, termed ‘inactive’) were mouse anti-human β1 integrin 4B4 (Beckman Coulter, 6603113) and rat anti-human β1 integrin mAb13 (generated in house from a hybridoma) ...
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bioRxiv - Bioengineering 2021Quote: The following antibodies were used for flow cytometry staining in a 96-well format: PE-labelled anti-human CD4 (Beckman Coulter, #A07752), APC-labelled anti-human CD8 (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...
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bioRxiv - Neuroscience 2020Quote: ... aggregated proteins were removed by centrifugation at 30,000×g for 20 min (Beckman Coulter, USA). Protein was concentrated by centrifugal spin filters (Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... green fluorescent protein (GFP)-positive cells were FACS-sorted (MoFlo Astrios, Beckman Coulter, California, USA) as single cells into a 96-well plate and clonally propagated ...
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bioRxiv - Cell Biology 2020Quote: ... A 1:1 Ampure XP (Beckman Coulter) PCR purification step was performed followed by assessment of size distribution using an Agilent high-sensitivity DNA chip (Agilent technologies ...
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bioRxiv - Genomics 2020Quote: The level of MPL protein on the platelet membrane was measured by flow cytometry (Beckman Coulter FC500) using the monoclonal antibodies ...
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bioRxiv - Immunology 2020Quote: CCMV-SS and CCMV-Ctt830-SS VLPs were separated from cellular proteins by ultracentrifugation (SW28 rotor, Beckman, Palo Alto ...
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bioRxiv - Immunology 2024Quote: Antibodies and clones against the following proteins were used (clone, fluorophore, company): CD3 (UCTH1, PECy5, Beckman Coulter), CD7 (Horizon V450 or BUV395 ...
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bioRxiv - Microbiology 2023Quote: ... The surface expression level of S proteins (Figure 4B) was measured using CytoFLEX Flow Cytometer (Beckman Coulter) and the data were analyzed using FlowJo software v10.7.1 (BD Biosciences) ...
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bioRxiv - Molecular Biology 2023Quote: ... supernatants from the SF samples and cellfree human and equine neutrophil cell culture supernatants were transferred to SW40 tubes (Beckman Coulter Inc., Brea, United States). They were subjected to centrifugation at 10,000 ×g for 35 min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The dialysed proteins were clarified by centrifugation in an Optima MAX-XP tabletop ultracentrifuge (Beckman Coulter Life Sciences) (90,720 x g ...
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bioRxiv - Cancer Biology 2022Quote: ... proteins enriched by IgG-agarose beads were eluted and processed with Agencourt AMPure-XP magnetic beads (Beckman Coulter) as previously described [40] ...
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bioRxiv - Cancer Biology 2020Quote: ... CD14 BV605 (1:100) and CD11b PC5 (1:100, Beckman Coulter). Cells were analyzed on the FACSCelesta (BD).
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bioRxiv - Systems Biology 2022Quote: ... and purification with Ampure XP beads (1:1 ratio, Beckman Coulter) produced a purified cDNA library ...
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bioRxiv - Genomics 2020Quote: ... cleaned up with 1:1 AMPureXP beads (Beckman Coulter, Brea, CA). ...
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bioRxiv - Plant Biology 2020Quote: ... purified with the double AMPure XP cleanup (1:1) (Beckman Coulter), and checked with a High sensitivity DNA chip (Agilent technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... Unbound protein was separated from liposomes by transferring 100μl of this mixture to 7×20mm PC ultracentrifuge tubes (Beckman) and carefully layering an equal volume of 0.75M sucrose buffer followed by 25μl of sucrose-free HK buffer ...
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bioRxiv - Cancer Biology 2020Quote: Cells expressing different fluorescent proteins were harvested from culture and counted using a ViCELL-XR (Beckman Coulter, Indianapolis, IN). Cells were washed 3 times in PBS to remove remaining cytokine ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of each standard protein were loaded on top of the gradient and centrifuged at 29.000 rpm in a SW41 rotor (Beckman) for 29 h at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... The gradients were loaded with 50 μg of recombinant CLASP2 C-term or protein standards of known sedimentation coefficient and centrifuged for 29 h at 29.000 rpm in a SW41 rotor (Beckman). Fractions of 700 μl were collected starting from the bottom of the tube using a peristaltic pump ...
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bioRxiv - Systems Biology 2021Quote: ... Cells expressing the DsRed fluorescent protein were FAC sorted with a MoFlo cell sorter (Beckman Coulter GmbH, Krefeld, Germany) to obtain a highly enriched fraction for neuronal cells.
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bioRxiv - Microbiology 2023Quote: ... The denatured protein fraction was then removed via ultracentrifugation at 100,000 g for 20 min at 4°C (Beckman Coulter Optima XE-90 – IVD ultracentrifuge with a 42.2 Ti rotor) ...
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bioRxiv - Microbiology 2021Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 μL of sterile water ...
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bioRxiv - Cancer Biology 2022Quote: ... was removed using right-side size selection with AMPure XP beads at a 1:1 and 7:1 bead:sample ratio (Beckman Coulter) described previously26 ...
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bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using 1:1 (vol: vol) AMPure XL beads (Beckman Coulter). cDNA prepared as above was end-prepped using NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... After cleanup with AMPure XP Reagent (Beckman Coulter, 1:1 ratio beads:PCR), iPCR amplification was carried out with KAPA HiFi HotStart ReadyMix (Roche) ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... PCR amplicons for pools 1 and 2 were combined for each sample and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30μL of sterile water ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 24 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 18 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...
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bioRxiv - Biochemistry 2021Quote: ... 300 μL of metabolite pool was aliquoted per target protein replicate and sealed with aluminum foil seals (Beckman Coulter 538619) to create the metabolite chambers ...
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bioRxiv - Biochemistry 2020Quote: ... Protein-liposome complexes were separated from unbound protein by centrifugation (2 h at 45.000 rpm, 4 °C, MLS50 rotor -Beckman Coulter) in the density gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... the extract and the protein ladder were overlaid onto separate gradients and centrifuged with an SW41Ti rotor (Beckman Coulter, 20U113807) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... and the uptake of endocytosed fluorescent proteins was analyzed in CD42b-positive population using a CytoFlex S flow Cytometer (Beckman).
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bioRxiv - Genomics 2021Quote: ... then re-purified with SPRI beads with 1:1 ratio (Beckman Coulter, B23317), and eluted in 25 µl of water (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... 750 µL of the 80% Histodenz solution were added and thoroughly mixed with 750µL of the protein-LUV sample in Thinwall centrifuge tubes (Beckman 50.2ti rotor). 1.5 mL of the 30% Histodenz solution was added as the intermediate layer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were clarified by centrifugation using a TLA-55 rotor in an Optima MAX-XP table top ultracentrifuge (Beckman Coulter) (90,720 x g ...
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bioRxiv - Immunology 2022Quote: ... and the expression of the Gag- p24 viral protein was detected with a PE-anti-p24 antibody (clone KC57 RD1; Beckman Coulter). Cell viability was determined using an aqua viability dye for flow cytometry (LIVE/DEAD® Fixable Aqua Dead Cell Stain kit ...
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bioRxiv - Biochemistry 2023Quote: The tendency of the protein to form aggregates was determined by dynamic light scattering (DLS) in the Delsa Max Core (Beckman Coulter). Prior to measurement ...
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bioRxiv - Pathology 2023Quote: ... the proportion of HIV-1-infected cells was determined by intracellular staining of the viral protein Gag-p24 with a Mouse anti-p24-FITC antibody (clone KC57, Beckman Coulter).
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...