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Citations for Beckman :
101 - 150 of 1268 citations for Ethyl 5 4 methoxyphenyl 5 oxovalerate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Linear sucrose gradients were prepared by layering 1.8 ml each of sucrose solutions (wt/wt; prepared in 5 mM EDTA, pH 7.5) into 14-by 95-mm ultracentrifuge tubes (Beckman) in the following order ...
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bioRxiv - Cancer Biology 2020Quote: ... equivalent amounts of ribosomes for each lysate was loaded onto each 10%-45% sucrose density gradient (BioComp Gradient Master machine) for ultracentrifugation at 33,000 rpm for 5 hours (Beckman Optima L80K Ultracentrifuge ...
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bioRxiv - Microbiology 2022Quote: ... Cells were grown for additional 1 hour at 30 °C and the biomass was harvested by centrifugation (10,000 rpm, for 5 minutes, JLA-10.500 rotor (Beckman Coulter)).
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bioRxiv - Genetics 2020Quote: ... 5 μL of each PCR reaction from the single worm lysate was pooled and purified by SPRI (Beckman Coutler) to remove unincorporated oligos ...
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bioRxiv - Immunology 2021Quote: ... Soluble CuMV-CP-containing protein solution was separated from the cellular proteins by ultracentrifugation in a sucrose gradient (20%-60% sucrose; ultracentrifugation at 25,000 rpm for 6 hours at 5°C [SW28 rotor, Beckman]). After dialysis CuMVTT-containing gradient fractions ...
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bioRxiv - Microbiology 2020Quote: ... The sample was vortexed for 10 s and incubated at room temperature for 5 minutes before centrifugation at 12,000 rpm (JA-15 1.5 rotor, Beckman Coulter) 4°C for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Resuspended pellets were then subjected to an iodixanol density gradient in 5-mL Ultra-Clear centrifuge tubes (Beckman Coulter). The tubes were first filled with a two-layer gradient ...
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (5 mg/ml) and floated for 2h at 55,000 rpm in a TLS-55 rotor (Beckman) at 25 °C through a sucrose gradient ...
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bioRxiv - Cell Biology 2022Quote: ... The stock solution of aggregate-depleted BSA (5% w/v) was prepared by overnight centrifugation at 100,000 x g (SW28 rotor Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... and 3.5 mL of 5% iodixanol in a 16.8 mL open top polyallomer tube (Beckman Coulter, Fullerton, California, USA). One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... respectively) were gently layered on top of each other in ultracentrifuge tubes (Ultra-Clear 5 mL Tube, Beckman Coulter) and a continuous gradient was generated using a gradient maker (Gradient Master ...
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bioRxiv - Microbiology 2024Quote: ... The phage was purified by isopycnic centrifugation in a CsCl discontinuous gradient for 5 h at 32,000 rpm in a SW41 rotor (Beckman) at 20°C ...
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bioRxiv - Neuroscience 2023Quote: ... after which the cells were harvested by centrifugation at 4,000 rpm for 30 min at 5°C using the JLA 8.1000 rotor (Beckman Coulter). The harvested pellets were stored at −20°C until use ...
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bioRxiv - Microbiology 2022Quote: ... the stained cells were detached by 5 mM of EDTA/PBS and analyzed with a CytoFLEX Flow Cytometer (Beckman). 10,000 events in a gated live cell population (based on SSC/FSC ...
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bioRxiv - Plant Biology 2023Quote: ... Cell debris was removed by centrifugation at 1,300 g for 5 min (Beckman Centrifuge Avanti® J-26-XP). Afterwards ...
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bioRxiv - Cancer Biology 2023Quote: ... Percentage of cells with each DNA content (2N, 3N, 4N, 5-8N) were estimated using CytExpert software (Beckman Coulter). For DNA content analysis using sorted cells ...
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bioRxiv - Molecular Biology 2024Quote: ... the CP was centrifuged at 72,000 rpm (280,000 × g) for 1 h at 5 °C in a TLA-100.3 rotor (Beckman Coulter, USA) on a TL-100 ultracentrifuge (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... the samples were centrifuged at 72,000 rpm (280,000 × g) for 1 h at 5 °C in a TL-100.1 rotor (Beckman Coulter, USA) on a TL-100 ultracentrifuge to pellet the reassembled VLPs ...
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bioRxiv - Neuroscience 2024Quote: ... but 2.5 mL for 5%) and ultracentrifuged at 100,000 x g (SW 40 Ti or SW41 Ti rotor, Beckman Coulter) for 16 h using ultra-clear tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... 10% and 0% in buffered sucrose on top of one another in 5 ml Ultra-Clear centrifuge tubes (Beckman). Gradients were centrifuged for 16 h at 100,000 x g and 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were subjected to two rounds of centrifugation at 18000 rpm for 5 and 30 mins in a JA-25.50 rotor (Beckman). Protein levels were normalized for coimmunoprecipitation and immunoblot analysis using a Bradford Assay ...
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bioRxiv - Genomics 2024Quote: ... Cell concentration was measured daily (particle size 5-8 um) using a Coulter counter (Coulter Z2, Beckman Coulter GmbH), in all experimental tanks ...
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bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Four mL of each density (3.5 mL for the 5% density) was stacked into an open polyallomer tube (337986, Beckman Coulter) using a Tecan freedom EVO ...
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bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5 × 105 additional cells were immediately processed for far red signal analysis by flow cytometry (Gallios, Beckman Coulter, Brea, USA) (D0) ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Microbiology 2020Quote: ... for 5 minutes using a FIBERLite F10BCI-6×500y rotor (Piramoon Technologies) in an Avanti JXN-30 centrifuge (Beckman Coulter). The clarified cell lysate was further centrifuged in a JS-24.38 rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... the 96-well plate was centrifuged at 1,000 RPM for 5 min using an Allegra 25R model centrifuge (Beckman Coulter) with inserts for 96-well plates ...
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bioRxiv - Microbiology 2020Quote: ... At the end of each selection cells were pelleted from the media by centrifugation 200 x g for 5 min in an Allegra centrifuge (Beckman) and supernatant was transferred for storage at −80C ...
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bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
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bioRxiv - Immunology 2022Quote: ... 1.5 μl of PI was also added and samples were run on the Gallios™ Flow Cytometer (Beckman Coulter, Inc). Data was analysed offline using Kaluza® Flow Analysis software version 2.1a (Beckman Coulter Inc. ...
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bioRxiv - Immunology 2021Quote: ... subsequently overlaid onto 5 mL of 20% sucrose and ultracentrifuged at 24,000 rpm for 2.5 hrs (Beckman XPN80, SW32.Ti). The viral pellet was resuspended in PBS.
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bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrated chromatin samples were loaded on 11 mL gradients of 5-25% sucrose in TE buffer and centrifuged in a preparative ultracentrifuge with a SW41 Ti rotor (Beckman) for 11 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were centrifuged at 1500 g for 5 min in an F2402 rotor in a GS15R table-top centrifuge (Beckman) and resuspend with water to wash the cells ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Microbiology 2023Quote: Human bladder cell line PD07i was cultured at 37°C with 5% CO2 to confluency as previously described and counted using a Coulter Counter (Beckman) to a minimum of 1×105 cells/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were then loaded onto a 5-50% sucrose density gradient and centrifuged in an SW41 Ti rotor (Beckman) at 36,000 rpm for 2 h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Sample analysis was performed by mixing 5 µl of fluorescent vesicles with 20 µl of Flow-Count Fluorospheres (Beckman Coulter) in a final volume of 200 µl of PBS to determine absolute counts ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM Mg(OAc)2) and centrifuged for 20 min at 100,000 x g in a 70Ti rotor (Beckman Coulter). The resulting membrane pellet was resuspended in 1 ml rough microsome (RM ...
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bioRxiv - Biophysics 2023Quote: ... Each 300μl dialysate was mixed with 300μl of 80% (w/v) Nycodenz dissolved in reconstitution buffer and then divided equally into two 5 x 41 mm ultracentrifuge tubes (Beckman). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysed cells were centrifuged and the viscous supernatant was immediately loaded on a 5-24% (w/v) sucrose density gradient and centrifuged in SW 32 Ti rotor (Beckman) overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysates were then loaded onto a 5–50% sucrose density gradient and centrifuged in an SW41 Ti rotor (Beckman) at 36,000 rpm for 2 h at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples then underwent a second Zymo DNA Clean & Concentrator-5 purification and were eluted in 30µl of elution buffer prior to a 1.2X AMPure XP bead (Beckman Coulter; A63881) cleanup ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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bioRxiv - Biophysics 2021Quote: ... transferred to 15 mL conical tube and centrifuged at 233 x g for 5 min at 22 °C using a swinging bucket rotor (Beckman Coulter). Pelleted cells were resuspended and washed once with 1x PBS (-CaCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... OptiPrep top to bottom density gradients 5 % to 40 % were performed as described66 in 12mL open top polyallomer tubes (Beckman Coulter). 1mL fractions were collected from the top ...